andrew gibbs bruker biospin ltd · • continue to invest heavily in innovation with ~10% annual...

Andrew Gibbs

Bruker BioSpin Ltd

Bruker News

Innovation with Integrity

Magnetic Resonance –

At the Heart of our Company

• Committed to serve the MR community

• Continue to invest heavily in innovation with ~10% annual R&D investment

• Provide scientists with

• Cutting-edge innovations: e.g. GHz NMR technology, DNP-NMR

• Highest performance NMR, EPR, DNP, preclinical MRI instruments

• Drug discovery & development p plus QC tools for Biopharma

• High-value analytical solutions for Applied, Industrial & Clinical Markets: e.g. NMR FoodScreener, Avance IVDr, Minispec TD-NMR

• First-class global support and service, & after-market services: LabScape™



• Foundation of a new prize by Bruker. The prize will be presented annually at the European Magnetic Resonance (EUROMAR) conference.

• The prize is awarded for outstanding magnetic resonance applications that have an impact beyond basic research.

• The 2016 Ernst Prize committee is comprised of Profs. Lucia Banci (chair), Marina Bennati, Lucio Frydman, Robert Griffin, Wiktor Kozminski, Vladimir Sklenar, and Thomas Vosegaard.

• 2016 prize-winner: Dr. Alex Pines, Berkeley, CA

• Next EUROMAR: Warsaw 2017

The Richard R. Ernst Prize for Magnetic Resonance

The Evolution of Magnetic Resonance

Productivity and Throughput

Bigger and Better New Applications

Innovation in

Magnetic

Resonance



• Active shielding reduces space requirements by > one order of magnitude

• Aeon 1 GHz magnets leverage advanced BEST superconductors

• Active refrigeration eliminates LN2, reduces LHe boil-off essentially to zero

World's first shielded Aeon 1 GHz System installed

Aeon 1 GHz at Research Center for Bio-Macromolecules at University of Bayreuth

Ascend Aeon™ 950 World’s First Single-Story 950 MHz Magnet

Ascend™ active-shielding technology • Minimum stray fields • External Disturbance Suppression (EDS) with 99%

screening efficiency • Single-story 950 MHz 54mm magnet • Requires min. 360cm/12’ ceiling height • UltraStabilized™ 2 Kelvin operation

Aeon™ active-refrigeration technology • He re-liquefaction with proprietary 2-stage Pulse

Tube Cooler (PTC) integration for no-compromise, high-resolution NMR

• LN2 free: no nitrogen refills ever • Essentially zero LHe consumption • Aeon Care maintenance plan


• Integrates Aeon refrigeration technology, obviates need for any cryogen refills

• Installed at University of Leeds, UK

Novel 15N-direct observe CryoProbe, further optimized for 15N, now offered up to 1GHz

• Can make direct 15N detection and 15-N TROSY advantageous in large globular proteins and in IDPs

• Favorable 15N relaxation properties at higher fields and larger molecular weights

• Active site information for paramagnetic metallo-proteins, where proton or even carbon magnetization is broadened beyond detection limits

New TXO or TCI CryoProbes with cold 15N preamplifiers now enable triple resonance experiments with direct 15N detection


3D (H)CbCaCON

New Applications:

Triple-Resonance 111 kHz MAS 1 GHz Probe Fully protonated 15N,13C Proteorhodopsin


Courtesy: Guido Pintacuda,

Daniela Lalli, ENS Lyon

● Twelve 263 GHz/ 400 MHz ● Ten 395 GHz/ 600 MHz ● Five 527 GHz/ 800 MHz

527 GHz/ 800 MHz

Solid-State DNP Spectrometers - a world-wide success

Now 27 installed: (Oct. 2016)

Solid-State DNP Spectrometer Gyrotron and Klystron Options

Work in Progress: 263 GHz Klystron

• Offer a lower-price more compact microwave source at 263 GHz

• Design compatible with possible upgrade later to gyrotron for full power performance

• Power output ~ 5 W • Available 2017

DNP with Gyrotron

DNP with Klystron Microwaves On

Microwaves Off

• Available for different rotor sizes: 3.2 mm (28 μL, 15 kHz @ 100 K) 1.9 mm (10 μL, 25 kHz @ 100 K) 1.3 mm ( 2 μL, 40 kHz @ 100 K)

• H/X/Y and H/X WB probes

• Low sample temperature and R.F. circuit ~100 K

• Cold insert/eject capability

• Individual heaters for VT, bearing

and drive gases for optimal temp. control

• Long term operation (days, weeks)

Low Temperature (LT) MAS DNP Probe

Broadband Diffusion probe - DiffBB

Preclinical Imaging Innovations New 3T Preclinical MRI System with Superior Cryogen-free Magnet and PET/MRI Option

• Novel, superior liquid cryogen-free 3 Tesla

magnet

• Magnet stays at field up to 4 hours during

power or cold water failure

• No liquid Helium or Nitrogen filling required

• Upgradable to PET/MRI


Phenome Centers: Collaborations drive

clinical metabolomics and IVD research by NMR

• Large-scale human metabonomics studies to build meaningful statistics and biomarker ID

• Bruker is NMR partner in growing global network of Phenome Centers and supplies NMR SOPs

• Phenome Centers are national/regional facilities offering access to world-class phenotyping resources

• Global network of 12 centers so far with Bruker as NMR partner

• Clinical Phenome Centers (CPC) at hospitals aim to enable better diagnoses, treatment and monitoring based on individual metabolic characteristics


NMR at MRC-NIHR UK National Phenome Center, opened in 2013

NMR at St.Mary‘s Hospital, London: Clinical Phenome Center opened in 2012

Latest Phenome Centre in Birmingham, UK

Honey Profiling Module

of the NMR FoodScreener™

• Quickly gaining acceptance by major global food analysis labs and honey-packers

• Eurofins Scientific Group and the Bruker-led Honey Consortium with companies QSI and ALNuMed harmonize NMR honey profiling models

• Famille Michaud Apiculteurs first honey-packer to adopt this novel NMR solution


Updated Wine Profiling Module of the

NMR FoodScreener™

• Increases number of regions covered, now includes France, Italy and Spain

• NMR FoodScreener™ laboratory at Bruker for food authenticity and quality determination granted ISO/IEC 17025 accreditation in 2015

• Authentication, fraud detection and labeling compliance

– Producers guaranteeing quality of their product

– Merchants anxious to protect investment and reputation

– Brand protection


• Small molecule APIs, complex mixtures and biologics

• ‘Complete Reduction to Amplitude Frequency Table’ (CRAFT) for targeted fingerprinting

• Tools for spectral DB creation

AssureNMR™ New Capabilities:

CRAFT, Biologics, HMDB,...


• Incorporates public data bases

– Human Metabolome Database (www.hmdb.ca)

– DrugBank (www.drugbank.ca)

• Quantitative 2D HSQC for biologics and complex mixtures.

SampleCase Heated

TopSpin 3.5pl6 New for all AVIII and AVIIIHD

GLP & 21CFR11

Features for regulated environments

TopSolids 2.0

Experiment Selector

Intuitive method selection

Organized by application WaveMaker

NMRSim New User Interface

IconNMR

CMC-assist, AssureSST,

WebInterface Automation

Non-Uniform Sampling

Free TopSpin™ Processing

www.bruker.com/FreeTopSpin


http://www.bruker.com/FreeTopSpin

Users Meeting 2016 21

A new module...

8.11.2016

Workflow: The set goal

• Insert sample

• Provide very little information about the sample

• Select experiments

• Wait… (yes it still takes time to measure)

• Click process

• Done.

Users Meeting 2016 22 8.11.2016

Sample Details

8.11.2016 Users Meeting 2016 23

Experiment setup

8.11.2016 Users Meeting 2016 24

3D NUS HNCACB in full automation

8.11.2016 Users Meeting 2016 25

Current state

• First version is part of TopSpin 3.5pl6

• Has about 80 experiments included (Proteins as a start)

• Possibility to add user experiments

• Fully automated processing up to 3 dimensions including pseudo3D and NUS.

If you have any ideas, problems, suggestions, feature requests…

Mail us: [email protected]

Users Meeting 2016 26 8.11.2016

Pharma (and industry) – time is money!

Drug Discovery

• New TS Tools support Fragment Based Screening analyses

• Complete Workflow: samplePro Tube, sampleJet, QCI/TCI, CMC_q & TS FBS tools!

Process Understanding and Reaction Optimisation

• Growing InsightMR Product Family with InsightXpress & InsightEPR

QC – Potency by qNMR

• A single experiment determines the amount of active compound in a drug!

• This fast, selective and reliable method frees the expert from routine tests.

QC – Quantification of components in solids mixtures

• Fast, “push-button”, “bench top”: Minispec mq20 + Dynamics Center

• Quantifies components based on their T1 Saturation recovery curve

Magnetic Resonance & Preclinical Imaging

Service and Life Cycle Support - LabScape

• New, flexible approach to instrument life-cycle management, maintenance & service

• Includes range of maintenance, repair and upgrade agreements for NMR, EPR, MRI and other preclinical imaging product lines

• Customers can choose comprehensive or à la carte services, as needed

• Customers can rely on LabScape for reliable, optimal instrument performance and ready access to up-to-date expertise and techniques from Bruker’s team of trained engineers and MR applications scientists


www.TheResonance.com: A new look to a very successful site!

www.TheResonance.com


http://www.theresonance.com/

Welcome to our Guest Speakers!


Dr Subrahdip

Paul

Nottingham

Prof Guy Lloyd-Jones FRS

Edinburgh

Dr R Flemming

Hansen, UCL

Dr Agnieszka

Szczygiel

AkzoNobel

andrew gibbs bruker biospin ltd · • continue to invest heavily in innovation with ~10% annual...

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