Analysis of lytic proteins pinholin and Rz/Rz1

Sue Parks and Lawangin Khan

2

S R Rz

Rz1

Lytic Cassette

3

Comparative analysis of Trans-Membrane domain Motifs in pinholin proteins

Two Domains in the Pinholin Protein

Pang et al.(2010) Mutational Analysis of S21 pinholin. Molecular Biology, 76(1): 66-77.

Figure 1D: Phage S21 reading frame

Traditional Holin Model

TM1

TM2

TM3

TMD2TMD1

Text

Motifs TMD1 TMD2MXXM

Comparative analysis of lysis protein, Rz1 cleavage

sitesLawangin Khan

Localization? Why Cleave?

Cleave site

Cleavage site conserved? Determining cleavage site

• Determined by LipoP, (www.cbs.dtu.dk/services/LipoP/)

Comparative Analysis

Relative Consensus and Position

• Krupovic’s lipobox consensus: L(A/S)(G/A)C