An Introduction To Lipidomic Workflows

John Bowden (University of Florida)Gavin E. Reid (University of Melbourne)Valentina Pirro (Eli Lilly and Company)

Course Outline

Sample Preparation Strategies

Mass Spec Analysis Workflow

Data Handling

Q&A

External Resources

Structural Identification

Introductory Level

Goal is to provide information andresources on current workflows, from theexperimental design stage to datadissemination, so that attendees can learnhow to adequately design, perform, andanalyze data from lipidomics experiments.

Basic knowledge of analytical chemistryand mass spectrometry is required

Overview of Lipid Biology

Imaging and datamatrix composition

Y

X

m/z

m/z 281

m/z 834m/z 788

LC Drift Time Ion Mobility MS*

*doi: 10.1021/acs.analchem.8b01527

*doi:10.1073/pnas.94.6.2339; doi:10.1039/C6AN00015K

*doi:10.1073/pnas.94.6.2339; doi:10.1039/C6AN00015K