an experimental vaccine against aeromonas hydrophila can
TRANSCRIPT
![Page 1: An Experimental Vaccine Against Aeromonas hydrophila can](https://reader031.vdocuments.site/reader031/viewer/2022020620/61e5e4c1435c295730336063/html5/thumbnails/1.jpg)
1
An Experimental Vaccine Against Aeromonas hydrophila can Induce Protection
in Rainbow trout Scott E. LaPatra Clear Springs Foods, Inc., Buhl,
Idaho, U.S.A.
Acknowledgements
! Karen Plant ! Bill Shewmaker ! Robin Burkhart ! Merle Higgins ! Andy Morton
![Page 2: An Experimental Vaccine Against Aeromonas hydrophila can](https://reader031.vdocuments.site/reader031/viewer/2022020620/61e5e4c1435c295730336063/html5/thumbnails/2.jpg)
2
![Page 3: An Experimental Vaccine Against Aeromonas hydrophila can](https://reader031.vdocuments.site/reader031/viewer/2022020620/61e5e4c1435c295730336063/html5/thumbnails/3.jpg)
3
![Page 4: An Experimental Vaccine Against Aeromonas hydrophila can](https://reader031.vdocuments.site/reader031/viewer/2022020620/61e5e4c1435c295730336063/html5/thumbnails/4.jpg)
4
Aeromonas hydrophila Vaccine Development
! Formalin killed, toxoid and live
attenuated
! Inject (adjuvant) vs bath
! Immunogenicity and delivery
potential of a lysate
! Development of a model system
![Page 5: An Experimental Vaccine Against Aeromonas hydrophila can](https://reader031.vdocuments.site/reader031/viewer/2022020620/61e5e4c1435c295730336063/html5/thumbnails/5.jpg)
5
Challenge Model Development Intraperitoneal Injection (IP)
Challenge Model Development Dorsal Sinus Injection (DS)
![Page 6: An Experimental Vaccine Against Aeromonas hydrophila can](https://reader031.vdocuments.site/reader031/viewer/2022020620/61e5e4c1435c295730336063/html5/thumbnails/6.jpg)
6
Challenge Model Development Lethal Dose 50% Determination
Susceptibility of Survivors Primary Challenge
! Triplicate 25-fish groups (mw, 7 g)
! 100 ul, 10(-1) thru 10(-4), 1.0 OD
! 16% cumulative mortality at
highest challenge dose
! No mortality at other doses
![Page 7: An Experimental Vaccine Against Aeromonas hydrophila can](https://reader031.vdocuments.site/reader031/viewer/2022020620/61e5e4c1435c295730336063/html5/thumbnails/7.jpg)
7
Susceptibility of Survivors Re-challenge, 10 g fish
Passive Immunization IP Delivery, 2 g fish
![Page 8: An Experimental Vaccine Against Aeromonas hydrophila can](https://reader031.vdocuments.site/reader031/viewer/2022020620/61e5e4c1435c295730336063/html5/thumbnails/8.jpg)
8
Vaccine Trial 4 wpv / 420 dd / 8 g
Aeromonas hydrophila Vaccine Development
! DS vs IP challenge procedure
! Effect of washing cells
! LD(50) determination
! Susceptibility of survivors
! Passive immunization
! Preliminary vaccine evaluation