validate spectrophotometric measurements with certified uv/vis reference materials

Reliable measurements – assured!

Validate spectrophotometric measurements and document their traceability with certified UV/Vis reference materials

• ISO 9001/17025 requires measurement devices to be checked on a regular basis

• GLP (good laboratory practice) governs the responsibility to provide high-quality, accurate data, including instructions for testing devices and reference materials

• Ph. Eur. “European Pharmacopoeia” and USP 857 requires any UV/Vis spectrophotometers in use to be qualified on a regular basis

• USP „U.S. Pharmacopeial Convention“ governs the verification of UV/Vis spectrophotometers

Using certified reference materials to check the measurement device guarantees:

High-quality measurement results

Traceability to international standards

UV/Vis electromagnetic spectrum:

The Hellma Analytics calibration laboratory is accredited for manufacturing and certifying UV/Vis reference materials from 190 to 890 nm

x-ray ultraviolet radiation visible light infrared

Electromagnetic radiation spectrum:

The following UV/Vis spectrophotometer parameters should be checked on a regular basis:

Photometric accuracy (absorbance)

Wavelength accuracy

Stray light level

Spectral resolution

Checking the photometric accuracy (absorbance)

… or with a Neutral Density Glass Filter

Wavelength in nm

I0 I

Light source Detector

using a Potassium Dichromate Liquid Filter

Checking the wavelength accuracy

… or with a Holmium Glass Filter

I0 I

Light source Detector

using a Holmium Liquid Filter

Wavelengths in nm

Checking wavelength and photometric accuracy using a Didymium Glass Filter

Wavelength in nm

Photometric accuracy :

Wavelength accuracy:

Wavelength in nm

I0 I

Light source Detector

Checking the stray light:

with Potassium Chloride (UV1), Sodium Iodide (UV10), Sodium Nitrite (UV11)

Checking spectral resolution using toluene in n-hexane

Wavelength in nm

I0 I

Light source Detector

Traceable

Reliable measurements through the use of certified reference materials.

SRMs

All reference materials certified by Hellma Analytics are traceable to NIST standards

Hellma calibrates its spectrophotometers with standard NIST reference materials on a daily basis

The Hellma Analytics calibration laboratory is:

...certified in accordance with ISO 17025

…Germany’s only accredited DAkkS calibration laboratory for UV/Vis reference materials

DAkkS - Calibration Certificate from Hellma Analytics

Reliability for Certified Reference Materials

In the DAkkS calibration certificate the measured values for each filter are documented.

DAkkS - Calibration Certificate from Hellma Analytics

Reliability for Certified Reference Materials

Glass filter sets for verification of the wavelength accuracy and/or photometric accuracy Quick and easy solutions for calibrating spectrophotometers

Type: 666-S000 Type: 666-S001 Type: 666-S002

Liquid filter sets Quick and easy solutions for calibrating spectrophotometers

Type: 667-UV100

Set for checking stray light in accordance with Ph. Eur.

Type: 667-UV200

Set for checking resolution in accordance with Ph. Eur.

Typ: 667-UV003

Complete set for performing checks in compliance with Ph. Eur.

Reference plate with holmium oxide strips for checking the wavelength accuracy and photometric accuracy of microplate readers The dimensions correspond to a standard microtest plate. Type: 666-R113

Reliable Measurements – Assured!

For further information, please visit: www.hellma-analytics.com/standards or contact us: sales.analytics@hellma.com