turning kinesin on the unconventional way
Post on 03-Jan-2016
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Turning Kinesin On the Unconventional Way
Jing Xu
Gross Lab, UC Irvine
• Coffee Maker
• Energy source
• Operation1. Turn machine on.
2. Use feature.
3. Turn machine off.
Macroscopic Machine
• Molecular Motor: Kinesin-1
• Energy source
• Operation?1. Turn machine on?
Vale, Cell (2003)
G ~ -0.5 eV ~ -20 kBT
+
Nano-Machine
Cargo
Hackney et. al, JBC (1992), Cai et. al, JCB (2007), Dietrich et. al, PNAS (2008), …
Autoinhibition“Off”
“Head”
“Tail”
Microtubule
Conventional Wisdom: Head-Tail Interaction
Active“On”
Cargo
Welte et. al, Cell (1998), Shubeita et. al, Cell (2008), Encalada et. al, Cell (2011)
Microtubule
Biology: There Exists Higher Order Regulation
Cargo
Active“On”
Inactive“Off”
Cargo
Xu et. al, Under Review
Microtubule
Tail-Independent Inactivation, Reversible
Active“On”
Cargo
Inactive“Off”
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