systematic identification of 3′-utr regulatory elements in activity-dependent mrna stability in...

Systematic identification of 3′-UTR regulatory elements in activity-dependent mRNA stability in hippocampal neurons

by Jonathan E. Cohen, Philip R. Lee, and R. Douglas Fields

Philosophical Transactions BVolume 369(1652):20130509

September 26, 2014

©2014 by The Royal Society

Activity-dependent, post-transcriptional gene regulation experiment.

Jonathan E. Cohen et al. Phil. Trans. R. Soc. B 2014;369:20130509

©2014 by The Royal Society

(Opposite.) Rapid, post-transcriptional regulation in hippocampal neurons mirrors activity-regulated genes in DRG axons.

Jonathan E. Cohen et al. Phil. Trans. R. Soc. B 2014;369:20130509

©2014 by The Royal Society

Discovery of enriched motifs for destabilized and stabilized genes. 3′-UTR analysis by MEME on downregulated (destabilized) and upregulated (stabilized) transcripts by MEME identifies miRNA-

binding sites.

Jonathan E. Cohen et al. Phil. Trans. R. Soc. B 2014;369:20130509

©2014 by The Royal Society

Predicted miRNAs are developmentally regulated in the hippocampus and may control nervous system development.

Jonathan E. Cohen et al. Phil. Trans. R. Soc. B 2014;369:20130509

©2014 by The Royal Society

miR-485 blocks NGF-induced neurite outgrowth in PC12 cells. miR-485 overexpression inhibits NGF-induced neurite outgrowth in PC12 cells.

Jonathan E. Cohen et al. Phil. Trans. R. Soc. B 2014;369:20130509

©2014 by The Royal Society

miR-485 decreases tau expression in hippocampal neurons.

Jonathan E. Cohen et al. Phil. Trans. R. Soc. B 2014;369:20130509

©2014 by The Royal Society

miR-485 overexpression controls tau expression and axonal outgrowth in hippocampal neurons. miR-485-5p overexpression reduced the extent of axonal outgrowth in hippocampal neurons.

Jonathan E. Cohen et al. Phil. Trans. R. Soc. B 2014;369:20130509

©2014 by The Royal Society