rongfeng cui, jiakun han, suzhen zhao, kunmei su, feng wu, xiaoqiu du, qijiang xu, kang chong,

Functional conservation and diversification of class E floral homeotic genes in rice (Oryza sativa)

Rongfeng Cui, Jiakun Han, Suzhen Zhao, Kunmei Su, Feng Wu, Xiaoqiu Du, Qijiang Xu, Kang Chong,Gu¨nter Theißen,* and Zheng Meng*

報告者 :劉育羣 N99H0011專討老師 :鄭伯智 林宏榮 醫師

日期 :05/08/2011

Oryza sativa 水稻 Sorghum bicolor 蜀黍

Hordeum vulgare 大麥 Triticum aestivum 小麥 Zea maysZea mays 玉玉米米

糧食作物中包括許多禾本科植物糧食作物中包括許多禾本科植物

• 中文名稱：水稻• 英文名稱： Rice• 學 名： Oryza sativa L.• 科名：禾本科 (Gramineae)• 別 名：稻仔• 原產地：中國長江流域。 1973 年在浙江省餘姚縣首次發

現約 7000 年前長江下游地區河姆渡文化遺址中有大量的稻穀遺存，證明當時已有農業生產，已開始種植水稻。

• 分布：台灣全島中低海拔水源充足，灌溉方便之平原。• 用途： 稻米以碾成白米供食用為主，是人類主食，次可

釀酒，並可製造米粉、米麩、蘿蔔糕等。• 特 性：水稻是台灣最重要的農作物，也是亞洲人民食用

的主食。水稻屬於禾本科單子葉的草本植物，喜歡溫暖多雨的氣候，台灣水稻一年可以栽種兩次，栽植水稻要先育秧苗，然後再移植到水田裏，約３至４個月可以收穫。我們吃的白米就是稻子的胚乳。

Oryza sativa 水稻

小穗由一朵小花組成，外由外穎及內穎包被，單生於穗柄上。

小穗→

OutlineOutline

•Introduction•Experimental Procedures•Results•Conclusions

Introduction

• The MADS-box family transcription factors play key roles in controlling development and signal transduction in eukaryotes.

• Phylogenetic analyses split the MADS-box gene family into two classes, termed type I (SRF-like) and type II (MEF2-like) genes.

MADS-box Gene FamilyMADS-box Gene Family

Messenguy and Dubois (2003)

Alvarez-Buylla et al. (2000)

de Folter S and Angenent GC (2006)

Soltis DE et al., (2007)

New Floral ABCE and Quartet Model

Se – sepal ( 花萼 )

Pe – petal ( 花瓣 )

St – stamen ( 雄蕊 )

Ca – carpel ( 心皮 )

Evolutionary implications of class Evolutionary implications of class E E genesgenes in rice in rice

The class E gene function specifying the identity of lodicules, stamens and carpels may require the direct interaction of OsMADS1 and OsMADS7/8 during spikeletmeristem development.

lemma (外穎 ) ， stamen (雄蕊 ) ， carpel (心皮 ) ， palea (內穎 ) ， lodicule (漿片 ) ， sterile lemma (護穎 )， pedicel(花梗 )

• Rice transformation• Scanning electron microscopy• In situ hybridization• RT-PCR and real-time PCR expression analysis• Yeast two-hybrid (Y2H) analyses• Coimmunoprecipitation (Co-IP) analyses of the ric

e SEP-like proteins in Arabidopsis mesophyll protoplasts

Experimental Procedures

Results

Molecular characterization of RNAi transgenic plants for rice SEP-ike genes

Phenotypes of wild-type and OsM7/8-RNAi spikelets

WT WTOsM7/8 -RNAi

OsM7/8-RNAi

OsM7/8 -RNAi

Scanning electron microscopy of epidermal cells expression patterns detailed structure

OsM7/8-RNAi

WT

OsM7/8 -RNAi

藉由這樣的實驗作者是要看小穗在 OsM7/8-RNAi 和 WT 當中，表皮細胞細部構造的

表現情形

Phenotypes of the OsM1/5/7/8-RNAi inflorescences and spikelets

WTOsM1/5/7/8-RNAi OsM1/5/7/8-RNAi

6 9

OsM1/5/7/8-RNAi

Under the Scanning electron microscopy the different developmental stages, formation

of inflorescence meristem changes

藉由這樣的實驗作者是要看小穗在 OsM1/5/7/8-RNAi 和在花序中產生變化所表現的

情形

Characteristics of the florets in transgenic lines

lodicule (漿片 )

stamen (雄蕊 ) carpel (心皮 )palea (內穎 )

lemma (外穎 )

此表格作者所要呈現的是轉殖株在花部位的特性

Expression patterns of OsMADS7 and OsMADS8 during inflorescence and spikelet development as revealed by in situ

hybridization

OsMADS7

OsMADS8 OsMADS7 OsMADS8

pbm代表主要分支

sbm代表次要分支smc代表分生組織細胞 ;tsn代表末端分生組織 ;lsm代

表橫向分生組織

OsMADS7

OsMADS8

OsMADS7 OsMADS8

Meristem at different times for each organ, in part on the show are different

藉由這樣的實驗作者是要看 OsMADS7.OsMADS8 在花序與小穗中分生組織表現的情形

fm代表分生組織

Yeast two-hybrid assays. Interaction patterns of the rice SEP-like proteins including OsMADS1, OsMADS5, OsMADS7 and

OsMADS8 are shown

藉由這樣的實驗作者證實了 OsMADS1.OsMADS7.OsMADS8 蛋白彼此之間的結合

Interaction analyses of four rice class E proteins in vivo

藉由這樣的實驗作者證實了水稻中 E class 蛋白彼此之間的結合能力

conclusion

• Silencing of both OsMADS7 and OsMADS8 causes severe morphological alterations of floral organs.

• Simultaneous silencing of OsMADS1, OsMADS5, OsMADS7 and OsMADS8 transforms all floral organs into leaf-like structures.

• OsMADS1, OsMADS7 and OsMADS8 can cross-interact to each other while OsMADS5 can not.

Thanks for your listening