respiratory diagnostics
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Respiratory Diagnostics
Brian J. Payne, DVM
Carthage Veterinary Service, Ltd.
The Presentation
• The actual presentation given at AASV is based on actual case studies.
• This PowerPoint presentation (based on PRRS diagnostics specifically) describes many of the laboratory tests that were utilized for these actual cases.
One more thing…
It has been an enormous benefit for me in a clinical setting to
understand each diagnostic test that I request so that I can better
interpret the results.
I hope this helps!
PRRS…Diagnostics
• Serology– PRRSv– PRRS Viral Antigens– PRRS Antibodies
• Tissue– PRRSv
• Other– Semen PRRSv
PRRS…Serology
• ELISA
• IFA
• PCR
• RFLP
• Genetic Sequencing
• SVN
PRRS…Indirect ELISA
• Enzyme-linked immunosorbent assay
• Extremely high sensitivity ~100%
• 99.5% specificity
• Quick to run
• Indirect method to determine antigen-specific PRRSv antibodies
Indirect ELISA…Process
• Polymer substrate
• Enzyme-labeled anti-porcine conjugate
•Serum sample with PRRSv/Viral antibodies
• Antigen
Indirect ELISA…Results
• Bound enzyme allows visual detection
• In-house tests usually (+) or (-)
• In-lab tests utilize optical densities to give continuous range of numbers University of AZ, 1993
S/P Ratio Pos/Neg Results010.8938 POSITIVE 1-0.002 Neg 02.0789 POSITIVE 12.815 POSITIVE 13.456 POSITIVE 10.089 Neg 0
0.5541 POSITIVE 11.272 POSITIVE 1
0.5865 POSITIVE 10.3701 Suspect 00.4712 POSITIVE 11.3387 POSITIVE 1
ResultsPRRS ELISA
Indirect ELISA...Results
• IDEXX HerdChek• Based on S/P ratio
– sample/positive
• Optical densities (continual range)
• < 0.3 = Negative• 0.3-0.4 = Suspect• > 0.3999 = Positive
Indirect ELISA…Antibody Detection
• PRRSv Ab detectable 9-13 days PI
• Peak at 30-50 days
• Rapid decline but still may be detectable in > 10 months (personal experience is > 2 years)
Indirect ELISA…Limitations
• Allows evidence of exposure to PRRSv
• However, no information on:– Length of exposure– Severity of infection– Which strain is present– Differentiation of vaccine and wild-type virus
• Variability of immune responses within a given population
PRRS…IFA• Indirect fluorescent antibody
• Specificity 99.5%
• Sensitivity (based on laboratory variation)– Culture media– Protocols– Incubation time– Cell types– Technician skill and subjectivity of results– Lab PRRSv genetic sim. to wild-type PRRSv
IFA…The Process
• Polymer substrate• PRRSv (lab) infected cells
• Serum with PRRSv Antibodies (IgG)
• Fluorescein-labeled anti-porcine antibody
IFA…The Results
• Magnitude of Ab titer can be determined unlike ELISA
Magar, R., 1993
IFA…The Results
• < 1:20 or 16 = negative• 1:20 or 16 or greater =
positive• Titer endpoints are
subjectively determined, therefore variation
Titer Pos/Neg Results011:80 Pos 0
<1:20 Neg 0null Neg 01:20 POSITIVE 11:40 POSITIVE 11:20 POSITIVE 11:20 POSITIVE 1
ResultsPRRS IFA
IFA…Antibody Detection
• IgG against PRRSv detectable 7-11 days PI
• Peak at 30-50 days
• Rapid decline but still may be detectable in 4-6 months
PRRS…PCR
• Polymerase Chain Reaction
• In vitro amplification of DNA– RNA of virus extracted from sample– Reverse transcriptase (RNA to DNA)– PCR amplifies DNA
• Target most conserved genes– ORF 6 and ORF 7
PCR…Process
RNA
Reverse Transcriptase
DNA
Heat Denaturing
Primersand
Cooling
Annealing
Taq +Nucleotides
CT G
A
Complementary Strands
30 Cycles
PCR…The ProcessAfter 30 cycles, 1000’s of replicates
Gel Electrophoresis(Colorimetric, Radioactive, Fluorometric)(-) (+)
PCR…Adv / DisAdv
• Rapid turnaround (RT-PCR) - 1-3 days
• High sensitivity and specificity
• Detects Ag…no need to wait for immune system response
• Detection determined by correlation b/w primers and PRRSv
• Not indicative of replicating virus
PRRS…RFLP
• Restriction Fragment Length Polymorphism
• Utilizes RT-PCR of ORF 5 gene– Envelop proteins– Prone to mutation
• 3 Restriction Enzymes Used– Their cutting patterns are assigned numbers– I.e. 1-4-2, 2-1-2, 2-6-2, 1-5-2
RFLP…Interpretation
• May differentiate between vaccine virus (2-5-2) and wild-type virus
• No indication of virulence
• No indication on cross-protection
• Change in RFLP can be from 1 base pair substitution…careful interpretation
• Little indication of homology (4%)
RFLP…Example
“Open reading frame 5 of PRRSV was sequenced.
This PRRSV has a predicted RFLP cut pattern of
2-5-2 and differs from each of the 3 modified-live
PRRS vaccine viruses (Ingelvac/RespPRRS,
Ingelvac ATP, PrimePac) by more than .5%.”
University of MN
RFLP…Example
“RFLP cut patterns are usually an inaccurate
measure of PRRSV relatedness.”
University of MN
PRRS Genetic Sequencing
• Provides exact nucleotide sequence of ORF
• Utilize ORF 5 b/c most variable
• Therefore, more informative than RFLP
• Dendograms (phylogenetic tree) can be made and homologies can be seen
ORF 5 Genetic SequencingATGTTGGAGAAATGCTTGACCGCGGGCTGTTGCTCGCGATTGCTTTCTTTGTGG
TGTATCGTGCCGTTCTGTTTTGCTGTGCTCGCCAACGCCAGCAACAACAGCAGC
TCCCATCTACAGCTGATTTACAACTTGACGCTATGTGAGCTGAATGGCACAGATT
GGCTAGCTAACAAATTTGATTGGGCAGTGGAGAGTTTTGTCATCTTTCCCGTTTT
GACTCACATTGTCTCCTATGGTGCCCTCACTACCAGCCATTTCCTTGACACAGTC
GCTTTAGTCACTGTGTCTACCGCCGGGTTTGTTCACGGGCGGTATGTCCTAAGT
AGCATCTACGCGGTCTGTGCCCTGGCTGCGTTGACTTGCTTCGTCATTAGGTTT
GCAAAGAATTGCATGTCCTGGCGCTACGCGTGTACCAGATATACCAACTTTCTT
CTGGACACTAAGGGCATACTCTATCGTTGGCGGTCGCCTGTCATCATAGAGAA
AAGGGGCAAAGTTGAGGTCGAAGGTCATCTGATCGACCTCAAAAGAGTTGTGC
TTGATGGTTCCGTGGCAACCCCTATAACCAGAGTTTCAGCGGAACAATGGGGTC
GTCCTTAG >550 Nucleotides
Percent Identity
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
1 99.7 99.5 88.7 99.3 82.8 94.2 98.8 99.2 99.2 89.2 98.8 88.7 89.4 98.8 98.8 99.0 92.9 87.7 85.7 94.2 86.7 85.7 88.7 89.4 88.4 88.7 89.1 89.1 88.9 94.7 99.2 99.2 99.5 89.4 89.6 89.6 89.4 91.4 91.4 89.7 91.0 91.4 91.4 95.9 89.2 84.9 86.9 85.6 89.1 99.7 86.9 1 D04-33870 Black Jack/Holtkai
2 0.3 99.8 89.1 99.3 83.3 94.5 99.0 99.2 99.5 89.6 99.2 89.1 89.7 99.2 99.2 99.3 93.4 88.1 86.1 94.5 87.1 86.1 89.1 89.7 88.7 89.1 89.4 89.4 89.2 95.0 99.5 99.5 99.8 89.7 89.9 89.9 89.7 91.7 91.7 90.0 91.4 91.7 91.7 96.2 89.2 84.9 87.1 85.7 89.4 100.0 87.1 2 D04-29274 Black Jack Pork
3 0.5 0.2 89.1 99.3 83.3 94.7 99.0 99.2 99.5 89.6 99.2 89.1 89.7 99.2 99.2 99.3 93.5 88.1 86.1 94.7 87.1 86.1 89.1 89.7 88.7 89.1 89.4 89.4 89.2 95.2 99.5 99.5 99.8 89.7 89.9 89.9 89.7 91.7 91.7 90.0 91.4 91.7 91.7 96.4 89.2 84.9 87.1 85.7 89.4 99.8 87.1 3 D04-49976 W eidner, Al
4 11.2 10.9 10.8 89.6 84.6 85.1 89.2 88.9 89.2 98.3 89.1 98.0 98.5 89.1 89.6 89.7 84.9 97.8 89.4 86.1 95.2 89.4 98.7 87.6 87.7 98.5 99.0 99.0 98.8 86.6 88.7 89.6 89.2 87.4 87.9 87.6 87.2 89.1 89.1 87.7 88.7 89.1 89.1 86.9 88.2 88.1 87.1 85.6 99.5 89.1 87.1 4 D06-21801(4-6) H&P
5 0.7 0.7 0.7 10.4 83.1 94.2 99.5 99.3 99.5 90.0 98.8 89.6 90.2 98.8 99.5 99.0 93.7 88.6 86.1 94.9 87.6 86.1 89.6 90.0 89.1 89.6 89.9 89.9 89.7 95.4 99.2 99.2 99.5 89.7 89.9 89.9 90.0 92.0 92.0 90.4 91.7 92.0 92.0 96.5 89.6 85.6 87.2 85.9 89.9 99.3 87.2 5 D06-16814(21-25) County Line Pork
6 17.5 17.0 17.0 15.9 17.0 81.9 82.8 82.9 82.9 84.2 83.6 84.1 84.1 83.3 83.1 83.7 82.4 84.4 83.4 82.6 82.8 83.4 85.1 83.3 83.1 84.1 84.6 84.6 84.4 82.4 83.3 83.7 83.4 82.8 83.1 82.9 83.1 83.7 83.7 83.3 83.9 83.7 83.7 83.3 82.8 82.6 87.9 86.2 84.6 83.3 87.9 6 D06-13563-22 Sand Ridge
7 5.6 5.2 5.0 15.0 5.4 17.8 93.9 93.5 94.9 85.7 94.2 85.6 85.9 93.9 94.0 94.4 97.8 84.4 82.6 98.2 82.9 82.6 85.2 87.4 86.4 84.9 85.4 85.4 85.2 98.7 94.7 94.2 94.5 86.7 86.9 86.9 87.2 88.2 88.2 87.6 88.1 88.2 88.2 97.8 87.1 82.4 84.9 83.4 85.4 94.5 84.9 7 D06-6000-1 Huftalin/Dreska
8 1.0 0.8 0.8 10.7 0.3 17.3 5.6 98.8 99.2 89.7 98.5 89.2 89.9 98.5 99.2 98.7 93.4 88.2 85.6 94.5 87.2 85.6 89.2 89.7 88.7 89.2 89.6 89.6 89.4 95.0 98.8 98.8 99.2 89.4 89.6 89.6 89.7 91.7 91.7 90.0 91.4 91.7 91.7 96.2 89.2 85.7 87.1 85.7 89.6 99.0 87.1 8 D06-3518(1-5) County Line Pork
9 0.5 0.5 0.5 10.7 0.3 16.8 5.6 0.7 99.0 89.4 98.7 88.9 89.6 98.7 98.8 98.8 92.7 87.9 85.9 93.9 86.9 85.9 88.9 89.4 88.6 88.9 89.2 89.2 89.1 94.4 99.0 99.0 99.3 89.2 89.4 89.4 89.4 91.4 91.4 89.7 91.0 91.4 91.4 95.7 88.7 84.2 87.1 85.7 89.2 99.2 87.1 9 D05-65768(1-5) County Line Pork
10 0.8 0.5 0.5 10.7 0.5 17.3 4.8 0.7 0.7 90.0 99.0 89.2 90.2 99.0 99.3 99.5 93.4 88.2 86.1 94.5 87.2 86.1 89.2 90.2 88.9 89.2 89.6 89.6 89.4 95.0 99.3 99.3 99.7 89.6 89.7 89.7 90.2 91.9 91.9 90.5 91.5 91.9 91.9 96.2 89.4 85.2 87.2 85.9 89.6 99.5 87.2 10 D05-56088(16-20) County Line Pork
11 11.1 10.7 10.7 1.7 10.3 16.4 14.3 10.5 10.5 10.1 89.6 98.3 99.8 89.6 90.0 89.6 85.2 97.8 89.7 86.6 96.2 89.7 99.0 87.7 87.4 99.0 99.3 99.3 99.2 86.9 89.2 90.0 89.7 87.1 87.4 87.2 87.6 88.9 88.9 87.9 88.6 88.9 88.9 87.2 87.4 87.7 87.7 86.2 98.5 89.6 87.7 11 D04-14781(1-5) Black Jack Pork
12 1.2 0.8 0.8 10.7 1.2 16.4 5.6 1.3 1.0 1.0 10.5 89.1 89.7 99.3 98.8 98.8 93.2 88.1 85.7 93.9 87.1 85.7 89.1 89.9 88.9 89.1 89.4 89.4 89.2 94.4 99.0 99.3 99.3 89.9 90.0 90.0 89.9 91.9 91.9 90.2 91.5 91.9 91.9 95.5 89.4 85.2 87.1 85.7 89.4 99.2 87.1 12 D04-21622 Future Pork
13 11.4 11.1 11.0 2.0 10.6 16.4 14.8 10.9 10.9 10.9 1.7 10.9 98.5 89.1 89.6 89.7 85.4 97.3 88.9 86.4 95.4 88.9 98.7 87.2 87.4 98.7 99.0 99.0 98.8 86.9 88.7 89.6 89.2 87.1 87.2 87.2 87.1 88.7 88.7 87.4 88.4 88.7 88.7 87.2 87.1 87.4 86.4 84.9 98.2 89.1 86.4 13 D04-17554(1-2) K&K BlackJack Pork
14 10.9 10.5 10.5 1.5 10.1 16.6 14.1 10.3 10.3 9.9 0.2 10.3 1.5 89.7 90.2 89.7 85.4 98.0 89.6 86.7 96.4 89.6 99.2 87.9 87.6 99.2 99.5 99.5 99.3 87.1 89.4 90.2 89.9 87.4 87.6 87.6 87.7 89.1 89.1 88.1 88.7 89.1 89.1 87.4 87.6 87.6 87.6 86.1 98.7 89.7 87.6 14 D04-16905(1-3) Walbexer Black Jack
15 1.2 0.8 0.8 10.7 1.2 16.8 5.9 1.3 1.0 1.0 10.5 0.7 10.9 10.3 98.8 98.8 92.7 88.1 85.6 93.9 87.1 85.6 89.1 89.4 88.4 89.1 89.4 89.4 89.2 94.4 99.0 99.0 99.3 89.6 89.7 89.7 89.4 91.5 91.5 89.7 91.2 91.5 91.5 95.5 89.1 85.2 86.9 85.6 89.4 99.2 86.9 15 D04-16408-1 Illini Swine
16 1.2 0.8 0.8 10.4 0.5 17.0 5.6 0.7 0.8 0.7 10.3 1.2 10.6 10.1 1.2 98.8 93.4 88.6 86.1 94.7 87.6 86.1 89.6 90.0 89.1 89.6 89.9 89.9 89.7 95.2 99.3 99.0 99.3 89.7 89.9 89.9 90.0 92.0 92.0 90.4 91.7 92.0 92.0 96.4 89.6 85.6 87.1 85.7 89.9 99.2 87.1 16 D04-33869 County Line Pork
17 1.0 0.7 0.7 10.5 1.0 16.6 5.4 1.2 0.8 0.5 10.3 1.2 10.7 10.1 1.2 1.2 93.2 88.2 86.7 94.0 87.2 86.7 89.7 90.2 89.2 89.7 90.0 90.0 89.9 94.5 99.2 99.2 99.5 89.9 90.0 90.0 90.2 91.9 91.9 90.5 91.5 91.9 91.9 95.7 89.4 85.4 87.1 85.7 90.0 99.3 87.1 17 D05-8868(4-6) County Line
18 6.9 6.5 6.3 15.2 6.3 17.4 2.2 6.5 6.7 6.5 15.0 6.9 15.0 14.8 7.2 6.5 6.7 84.2 82.1 96.7 82.8 82.1 85.1 86.7 86.2 84.7 85.2 85.2 85.1 97.2 93.2 93.0 93.4 86.4 86.6 86.6 86.6 87.9 87.9 86.9 87.7 87.9 87.9 96.5 86.2 81.8 84.2 82.8 85.2 93.4 84.2 18 D05-46346(7-9) S&S Farms
19 11.0 10.6 10.6 1.0 10.2 14.8 14.5 10.4 10.4 10.4 0.8 10.4 1.4 0.7 10.4 10.2 10.4 14.7 88.9 85.4 95.4 88.9 98.0 86.6 87.2 98.0 98.3 98.3 98.2 85.9 88.1 88.6 88.2 86.6 86.7 86.7 86.4 88.4 88.4 86.7 88.1 88.4 88.4 86.2 86.9 87.4 87.1 85.6 98.0 88.1 87.1 19 D03-18981(1,2,4,5) Black Jack Pork
20 14.1 13.7 13.7 11.5 13.7 17.2 17.8 13.9 13.5 13.7 10.9 13.9 12.0 11.2 13.9 13.7 13.1 18.2 10.3 83.3 88.7 100.0 89.4 86.6 85.9 89.4 89.7 89.7 89.9 83.4 86.2 85.9 86.2 86.9 87.2 87.1 86.4 87.4 87.4 86.6 87.2 87.4 87.4 83.7 85.7 87.2 84.2 82.8 89.6 86.1 84.2 20 D03-18980(5,8) Black Jack Pork
21 5.4 5.0 4.8 13.9 4.8 17.1 1.9 5.0 5.2 5.0 13.7 5.7 13.9 13.5 5.7 5.0 5.6 3.2 13.5 17.4 84.1 83.3 86.2 87.7 86.9 85.9 86.4 86.4 86.2 99.3 94.7 94.2 94.5 87.4 87.6 87.6 87.6 88.9 88.9 87.9 88.7 88.9 88.9 98.5 87.6 82.9 84.6 83.1 86.4 94.5 84.6 21 D03-19304(20-23) S&S Farms
22 9.9 9.6 9.5 1.6 9.1 14.8 13.7 9.4 9.4 9.4 0.7 9.4 1.4 0.5 9.4 9.1 9.4 13.9 0.7 9.0 12.6 88.7 96.0 85.7 86.6 95.9 96.4 96.4 96.2 84.4 86.7 87.6 87.2 85.6 85.7 85.7 85.6 87.6 87.6 85.9 87.6 87.6 87.6 84.7 86.2 85.4 85.1 83.3 95.4 87.1 85.1 22 D03-16034(1-7-9) Black Jack Pork
23 14.1 13.7 13.7 11.5 13.7 17.2 17.8 13.9 13.5 13.7 10.9 13.9 12.0 11.2 13.9 13.7 13.1 18.2 10.3 0.0 17.4 9.0 89.4 86.6 85.9 89.4 89.7 89.7 89.9 83.4 86.2 85.9 86.2 86.9 87.2 87.1 86.4 87.4 87.4 86.6 87.2 87.4 87.4 83.7 85.7 87.2 84.2 82.8 89.6 86.1 84.2 23 D03-16035(5-8) Black Jack Pork
24 11.5 11.1 11.1 1.3 10.7 15.5 15.0 10.9 10.9 10.9 1.0 10.9 1.3 0.8 10.9 10.7 10.7 15.2 0.7 11.6 13.9 0.9 11.6 87.7 87.7 99.3 99.7 99.7 99.5 86.7 88.7 89.6 89.2 87.6 87.7 87.7 87.6 89.2 89.2 87.9 88.9 89.2 89.2 87.1 87.7 87.7 87.4 85.9 98.8 89.1 87.4 24 D03-14810-23 Black Jack Pork
25 10.9 10.5 10.5 12.8 10.1 17.7 13.2 10.3 10.5 9.9 12.6 10.3 13.4 12.4 10.7 10.1 10.1 14.0 12.5 14.6 12.9 11.5 14.6 12.8 95.7 87.6 87.9 87.9 87.7 87.9 89.9 89.9 89.9 95.5 95.7 95.7 99.3 97.8 97.8 99.5 97.7 97.8 97.8 88.2 96.0 87.2 85.4 84.1 87.9 89.7 85.4 25 D03-13137(11-15) Illini Farm 1904
26 11.3 10.9 10.9 11.7 10.5 16.5 14.0 10.7 10.7 10.7 11.9 10.7 12.3 11.7 11.1 10.5 10.5 14.2 11.2 14.0 13.3 10.0 14.0 11.7 3.6 87.6 88.1 88.1 87.9 87.1 88.9 88.9 88.9 94.2 94.4 94.4 95.7 96.7 96.7 95.9 96.8 96.7 96.7 87.6 94.5 86.2 84.7 83.1 88.1 88.7 84.7 26 D03-10907-3 LincolnLand Farms
27 11.4 11.1 11.0 1.3 10.6 16.4 15.2 10.9 10.9 10.9 1.0 10.9 1.3 0.8 10.9 10.6 10.7 15.4 0.7 11.5 14.1 0.9 11.5 0.7 13.0 11.9 99.7 99.7 99.5 86.4 88.7 89.6 89.2 87.4 87.2 87.4 87.4 89.1 89.1 87.7 88.7 89.1 89.1 86.7 87.4 87.9 86.9 85.7 98.8 89.1 86.9 27 D03-10033 Black Jack Pork
28 11.1 10.7 10.7 1.0 10.3 15.9 14.8 10.5 10.5 10.5 0.7 10.5 1.0 0.5 10.5 10.3 10.3 15.0 0.3 11.2 13.7 0.5 11.2 0.3 12.6 11.5 0.3 100.0 99.8 86.9 89.1 89.9 89.6 87.7 87.9 87.9 87.7 89.4 89.4 88.1 89.1 89.4 89.4 87.2 87.9 88.1 87.2 85.7 99.2 89.4 87.2 28 D03-10529(1-4) Black Jack Pork
29 11.1 10.7 10.7 1.0 10.3 15.9 14.8 10.5 10.5 10.5 0.7 10.5 1.0 0.5 10.5 10.3 10.3 15.0 0.3 11.2 13.7 0.5 11.2 0.3 12.6 11.5 0.3 0.0 99.8 86.9 89.1 89.9 89.6 87.7 87.9 87.9 87.7 89.4 89.4 88.1 89.1 89.4 89.4 87.2 87.9 88.1 87.2 85.7 99.2 89.4 87.2 29 D03-10935(1-4) Black Jack Pork
30 11.3 10.9 10.9 1.2 10.5 16.2 15.0 10.7 10.7 10.7 0.8 10.7 1.2 0.7 10.7 10.5 10.5 15.2 0.5 10.9 13.9 0.7 10.9 0.5 12.8 11.7 0.5 0.2 0.2 86.7 88.9 89.7 89.4 87.9 88.1 88.1 87.6 89.2 89.2 87.9 88.9 89.2 89.2 87.1 87.7 87.9 87.1 85.6 99.0 89.2 87.1 30 D03-10530-1 Black Jack Pork
31 4.8 4.5 4.3 13.5 4.3 17.4 1.3 4.5 4.7 4.5 13.3 5.2 13.5 13.1 5.2 4.5 5.0 2.7 13.1 16.9 0.7 12.2 16.9 13.5 12.8 13.1 13.7 13.3 13.3 13.5 95.2 94.7 95.0 87.6 87.7 87.7 87.7 89.1 89.1 88.1 88.9 89.1 89.1 99.0 87.7 83.4 85.1 83.6 86.9 95.0 85.1 31 D03-6293-4 S&S Farms (102850)
32 0.8 0.5 0.5 11.1 0.8 16.8 5.2 1.0 0.7 0.7 10.9 1.0 11.3 10.7 1.0 0.7 0.8 6.5 10.6 13.5 5.0 9.8 13.5 11.3 10.3 10.7 11.3 10.9 10.9 11.1 4.5 99.3 99.7 89.9 90.0 90.0 89.9 91.9 91.9 90.2 91.5 91.9 91.9 96.2 89.6 85.2 87.2 85.9 89.1 99.5 87.2 32 D02-48860(4-8) Crescent Pork
33 0.8 0.5 0.5 10.3 0.8 16.4 5.6 1.0 0.7 0.7 10.1 0.7 10.5 9.9 1.0 1.0 0.8 6.9 10.0 13.9 5.4 9.0 13.9 10.5 10.3 10.7 10.5 10.1 10.1 10.3 4.8 0.7 99.7 89.9 90.0 90.0 89.9 91.9 91.9 90.2 91.5 91.9 91.9 95.9 89.4 84.7 87.2 85.9 89.9 99.5 87.2 33 D02-45742-1 Black Jack Pork
34 0.5 0.2 0.2 10.7 0.5 16.8 5.2 0.7 0.3 0.3 10.5 0.7 10.9 10.3 0.7 0.7 0.5 6.5 10.4 13.5 5.0 9.4 13.5 10.9 10.3 10.7 10.9 10.5 10.5 10.7 4.5 0.3 0.3 89.9 90.0 90.0 89.9 91.9 91.9 90.2 91.5 91.9 91.9 96.2 89.4 85.1 87.2 85.9 89.6 99.8 87.2 34 D02-45742-2 Black Jack Pork
35 10.9 10.5 10.5 12.7 10.5 17.7 13.8 10.8 10.6 10.7 13.0 10.3 13.4 12.8 10.7 10.5 10.5 14.5 12.3 13.6 13.2 11.7 13.6 12.8 4.5 4.8 13.0 12.5 12.5 12.3 13.0 10.3 10.3 10.3 99.5 99.7 95.5 96.8 96.8 95.7 96.7 96.8 96.8 87.9 94.5 86.2 84.4 82.9 87.7 89.7 84.4 35 D04-49972-1 Illini Swine Inc
36 10.9 10.5 10.5 12.3 10.5 17.6 13.8 10.7 10.5 10.7 12.9 10.3 13.3 12.7 10.7 10.5 10.5 14.4 12.3 13.5 13.1 11.6 13.5 12.7 4.5 4.8 12.9 12.5 12.5 12.3 12.9 10.3 10.3 10.3 0.3 99.7 95.7 97.0 97.0 95.9 96.8 97.0 97.0 88.1 94.7 86.4 84.9 83.3 87.9 89.9 84.9 36 D04-49972-2 Illini Swine Inc
37 10.9 10.5 10.5 12.7 10.5 17.6 13.8 10.7 10.5 10.7 12.9 10.3 13.3 12.7 10.7 10.5 10.5 14.5 12.3 13.5 13.2 11.6 13.5 12.7 4.5 4.8 12.9 12.5 12.5 12.3 13.0 10.3 10.3 10.3 0.2 0.3 95.7 97.0 97.0 95.9 96.8 97.0 97.0 88.1 94.7 86.4 84.6 82.9 87.9 89.9 84.6 37 D04-49972-3 Illini Swine Inc
38 10.9 10.5 10.5 12.9 10.1 17.9 13.2 10.4 10.6 9.9 12.8 10.3 13.6 12.5 10.7 10.1 10.1 14.3 12.7 14.8 13.0 11.6 14.8 13.0 0.7 3.6 13.2 12.8 12.8 13.0 12.8 10.3 10.3 10.3 4.5 4.5 4.5 97.8 97.8 99.5 97.7 97.8 97.8 88.1 95.2 86.9 85.2 84.1 87.7 89.7 85.2 38 D03-6295-3 Illini-North
39 9.1 8.8 8.8 11.1 8.4 16.8 12.6 8.6 8.8 8.6 11.3 8.6 11.7 11.1 9.0 8.4 8.8 13.2 10.8 13.1 11.9 9.8 13.1 11.1 2.2 2.7 11.3 10.9 10.9 11.1 11.7 8.6 8.6 8.6 2.9 2.9 2.9 2.2 100.0 98.0 99.8 100.0 100.0 89.7 96.2 88.1 86.2 84.7 89.4 91.7 86.2 39 D03-5293(2-4) I llini Swine-Hich Tec
40 9.1 8.8 8.8 11.1 8.4 16.8 12.6 8.6 8.8 8.6 11.3 8.6 11.7 11.1 9.0 8.4 8.8 13.2 10.8 13.1 11.9 9.8 13.1 11.1 2.2 2.7 11.3 10.9 10.9 11.1 11.7 8.6 8.6 8.6 2.9 2.9 2.9 2.2 0.0 98.0 99.8 100.0 100.0 89.7 96.2 88.1 86.2 84.7 89.4 91.7 86.2 40 D03-5293(5-7) I llini Swine-High Tec
41 10.3 10.0 9.9 12.3 9.5 17.5 12.6 9.8 10.0 9.4 12.2 9.8 13.0 11.9 10.2 9.5 9.6 13.6 12.1 14.4 12.4 11.0 14.4 12.4 0.3 3.2 12.5 12.2 12.2 12.4 12.2 9.8 9.8 9.8 4.1 4.1 4.1 0.3 1.9 1.9 97.8 98.0 98.0 88.4 95.7 87.4 85.6 84.2 88.1 90.0 85.6 41 D03-5296(3-4) I llini Swine-North
42 9.3 9.0 8.9 11.3 8.6 16.6 12.8 8.8 9.0 8.8 11.5 8.8 11.9 11.3 9.2 8.6 9.0 13.4 11.1 13.3 12.1 9.8 13.3 11.3 2.4 2.5 11.5 11.1 11.1 11.3 12.0 8.8 8.8 8.8 3.1 3.1 3.1 2.4 0.2 0.2 2.0 99.8 99.8 89.6 96.0 87.7 85.9 84.4 89.1 91.4 85.9 42 D02-61015-3 Illini Farms South
43 9.1 8.8 8.8 11.1 8.4 16.8 12.6 8.6 8.8 8.6 11.3 8.6 11.7 11.1 9.0 8.4 8.8 13.2 10.8 13.1 11.9 9.8 13.1 11.1 2.2 2.7 11.3 10.9 10.9 11.1 11.7 8.6 8.6 8.6 2.9 2.9 2.9 2.2 0.0 0.0 1.9 0.2 100.0 89.7 96.2 88.1 86.2 84.7 89.4 91.7 86.2 43 D02-61016-1 Illini Farms South
44 9.1 8.8 8.8 11.1 8.4 16.8 12.6 8.6 8.8 8.6 11.3 8.6 11.7 11.1 9.0 8.4 8.8 13.2 10.8 13.1 11.9 9.8 13.1 11.1 2.2 2.7 11.3 10.9 10.9 11.1 11.7 8.6 8.6 8.6 2.9 2.9 2.9 2.2 0.0 0.0 1.9 0.2 0.0 89.7 96.2 88.1 86.2 84.7 89.4 91.7 86.2 44 D02-48857(5-8) I llini South
45 4.1 3.8 3.6 13.5 3.6 16.9 2.2 3.8 4.0 3.8 13.3 4.5 13.5 13.1 4.5 3.8 4.3 3.4 13.0 16.9 1.5 12.2 16.9 13.5 12.5 12.7 13.7 13.3 13.3 13.5 1.0 3.8 4.1 3.8 12.8 12.7 12.8 12.6 11.1 11.1 12.0 11.3 11.1 11.1 87.9 83.6 85.6 84.1 87.2 96.2 85.6 45 D02-48858(7-9) S&S Farms
46 10.9 10.9 10.9 12.2 10.5 18.0 13.4 10.8 11.0 10.7 12.8 10.7 13.4 12.6 11.1 10.5 10.9 14.3 12.1 15.3 13.0 10.9 15.3 12.6 3.8 4.5 12.8 12.4 12.4 12.6 12.8 10.7 10.7 10.7 5.2 5.2 5.2 4.5 3.6 3.6 4.0 3.8 3.6 3.6 12.8 87.9 85.6 83.9 87.9 89.2 85.6 46 D02-47395(1-2) Nipco Farms
47 14.5 14.5 14.5 12.3 14.1 18.5 17.5 13.7 14.6 14.3 13.2 14.3 13.6 13.4 14.3 14.1 14.3 18.0 12.1 13.2 16.9 12.5 13.2 13.2 13.5 14.1 12.9 12.8 12.8 13.0 16.5 14.3 14.7 14.3 14.4 14.4 14.4 13.7 12.7 12.7 13.1 12.9 12.7 12.7 16.9 12.3 84.2 83.1 88.1 84.9 84.2 47 D02-47394(5-8) Prime Pork
48 13.5 13.3 13.3 13.3 13.1 12.8 15.6 13.1 12.9 13.1 12.5 13.1 13.7 12.7 13.1 13.3 12.9 16.5 12.0 16.4 16.2 12.4 16.4 12.9 14.1 14.3 13.3 12.9 12.9 13.1 15.6 13.1 13.1 13.1 15.2 15.2 15.2 14.3 13.7 13.7 13.7 13.9 13.7 13.7 15.6 14.2 16.5 98.7 87.2 87.1 100.0 48 D06-28296-5 Boars
49 13.9 13.7 13.7 13.7 13.5 13.2 16.0 13.5 13.3 13.5 12.8 13.5 14.1 13.1 13.5 13.7 13.3 16.9 12.4 16.6 16.7 12.8 16.6 13.3 14.5 14.7 13.3 13.3 13.3 13.5 16.0 13.5 13.5 13.5 15.7 15.6 15.6 14.5 14.1 14.1 14.1 14.3 14.1 14.1 16.0 14.6 16.7 0.2 85.7 85.7 98.7 49 D06-26717-1 Boars
50 11.0 10.7 10.6 0.5 10.2 15.7 14.7 10.5 10.5 10.5 1.5 10.5 1.9 1.3 10.5 10.2 10.3 15.0 0.8 11.3 13.7 1.4 11.3 1.2 12.5 11.5 1.2 0.8 0.8 1.0 13.3 10.9 10.1 10.5 12.5 12.5 12.5 12.7 10.9 10.9 12.1 11.1 10.9 10.9 13.3 12.4 12.5 13.1 13.5 89.4 87.2 50 IngelvacATP
51 0.3 0.0 0.2 10.9 0.7 17.0 5.2 0.8 0.5 0.5 10.7 0.8 11.1 10.5 0.8 0.8 0.7 6.5 10.6 13.7 5.0 9.6 13.7 11.1 10.5 10.9 11.1 10.7 10.7 10.9 4.5 0.5 0.5 0.2 10.5 10.5 10.5 10.5 8.8 8.8 10.0 9.0 8.8 8.8 3.8 10.9 14.5 13.3 13.7 10.7 87.1 51 RespPRRS
52 13.5 13.3 13.3 13.3 13.1 12.8 15.6 13.1 12.9 13.1 12.5 13.1 13.7 12.7 13.1 13.3 12.9 16.5 12.0 16.4 16.2 12.4 16.4 12.9 14.1 14.3 13.3 12.9 12.9 13.1 15.6 13.1 13.1 13.1 15.2 15.2 15.2 14.3 13.7 13.7 13.7 13.9 13.7 13.7 15.6 14.2 16.5 0.0 0.2 13.1 13.3 52 D06-30617-1 FuturePork
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Percent Identity
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
1 99.7 99.5 88.7 99.3 82.8 94.2 98.8 99.2 99.2 89.2 98.8 88.7 89.4 98.8 98.8 99.0 92.9 87.7 85.7 94.2 86.7 85.7 88.7 89.4 88.4 88.7 89.1 89.1 88.9 94.7 99.2 99.2 99.5 89.4 89.6 89.6 89.4 91.4 91.4 89.7 91.0 91.4 91.4 95.9 89.2 84.9 86.9 85.6 89.1 99.7 86.9 1 D04-33870 Black Jack/Holtkai
2 0.3 99.8 89.1 99.3 83.3 94.5 99.0 99.2 99.5 89.6 99.2 89.1 89.7 99.2 99.2 99.3 93.4 88.1 86.1 94.5 87.1 86.1 89.1 89.7 88.7 89.1 89.4 89.4 89.2 95.0 99.5 99.5 99.8 89.7 89.9 89.9 89.7 91.7 91.7 90.0 91.4 91.7 91.7 96.2 89.2 84.9 87.1 85.7 89.4 100.0 87.1 2 D04-29274 Black Jack Pork
3 0.5 0.2 89.1 99.3 83.3 94.7 99.0 99.2 99.5 89.6 99.2 89.1 89.7 99.2 99.2 99.3 93.5 88.1 86.1 94.7 87.1 86.1 89.1 89.7 88.7 89.1 89.4 89.4 89.2 95.2 99.5 99.5 99.8 89.7 89.9 89.9 89.7 91.7 91.7 90.0 91.4 91.7 91.7 96.4 89.2 84.9 87.1 85.7 89.4 99.8 87.1 3 D04-49976 W eidner, Al
4 11.2 10.9 10.8 89.6 84.6 85.1 89.2 88.9 89.2 98.3 89.1 98.0 98.5 89.1 89.6 89.7 84.9 97.8 89.4 86.1 95.2 89.4 98.7 87.6 87.7 98.5 99.0 99.0 98.8 86.6 88.7 89.6 89.2 87.4 87.9 87.6 87.2 89.1 89.1 87.7 88.7 89.1 89.1 86.9 88.2 88.1 87.1 85.6 99.5 89.1 87.1 4 D06-21801(4-6) H&P
5 0.7 0.7 0.7 10.4 83.1 94.2 99.5 99.3 99.5 90.0 98.8 89.6 90.2 98.8 99.5 99.0 93.7 88.6 86.1 94.9 87.6 86.1 89.6 90.0 89.1 89.6 89.9 89.9 89.7 95.4 99.2 99.2 99.5 89.7 89.9 89.9 90.0 92.0 92.0 90.4 91.7 92.0 92.0 96.5 89.6 85.6 87.2 85.9 89.9 99.3 87.2 5 D06-16814(21-25) County Line Pork
6 17.5 17.0 17.0 15.9 17.0 81.9 82.8 82.9 82.9 84.2 83.6 84.1 84.1 83.3 83.1 83.7 82.4 84.4 83.4 82.6 82.8 83.4 85.1 83.3 83.1 84.1 84.6 84.6 84.4 82.4 83.3 83.7 83.4 82.8 83.1 82.9 83.1 83.7 83.7 83.3 83.9 83.7 83.7 83.3 82.8 82.6 87.9 86.2 84.6 83.3 87.9 6 D06-13563-22 Sand Ridge
7 5.6 5.2 5.0 15.0 5.4 17.8 93.9 93.5 94.9 85.7 94.2 85.6 85.9 93.9 94.0 94.4 97.8 84.4 82.6 98.2 82.9 82.6 85.2 87.4 86.4 84.9 85.4 85.4 85.2 98.7 94.7 94.2 94.5 86.7 86.9 86.9 87.2 88.2 88.2 87.6 88.1 88.2 88.2 97.8 87.1 82.4 84.9 83.4 85.4 94.5 84.9 7 D06-6000-1 Huftalin/Dreska
8 1.0 0.8 0.8 10.7 0.3 17.3 5.6 98.8 99.2 89.7 98.5 89.2 89.9 98.5 99.2 98.7 93.4 88.2 85.6 94.5 87.2 85.6 89.2 89.7 88.7 89.2 89.6 89.6 89.4 95.0 98.8 98.8 99.2 89.4 89.6 89.6 89.7 91.7 91.7 90.0 91.4 91.7 91.7 96.2 89.2 85.7 87.1 85.7 89.6 99.0 87.1 8 D06-3518(1-5) County Line Pork
9 0.5 0.5 0.5 10.7 0.3 16.8 5.6 0.7 99.0 89.4 98.7 88.9 89.6 98.7 98.8 98.8 92.7 87.9 85.9 93.9 86.9 85.9 88.9 89.4 88.6 88.9 89.2 89.2 89.1 94.4 99.0 99.0 99.3 89.2 89.4 89.4 89.4 91.4 91.4 89.7 91.0 91.4 91.4 95.7 88.7 84.2 87.1 85.7 89.2 99.2 87.1 9 D05-65768(1-5) County Line Pork
10 0.8 0.5 0.5 10.7 0.5 17.3 4.8 0.7 0.7 90.0 99.0 89.2 90.2 99.0 99.3 99.5 93.4 88.2 86.1 94.5 87.2 86.1 89.2 90.2 88.9 89.2 89.6 89.6 89.4 95.0 99.3 99.3 99.7 89.6 89.7 89.7 90.2 91.9 91.9 90.5 91.5 91.9 91.9 96.2 89.4 85.2 87.2 85.9 89.6 99.5 87.2 10 D05-56088(16-20) County Line Pork
11 11.1 10.7 10.7 1.7 10.3 16.4 14.3 10.5 10.5 10.1 89.6 98.3 99.8 89.6 90.0 89.6 85.2 97.8 89.7 86.6 96.2 89.7 99.0 87.7 87.4 99.0 99.3 99.3 99.2 86.9 89.2 90.0 89.7 87.1 87.4 87.2 87.6 88.9 88.9 87.9 88.6 88.9 88.9 87.2 87.4 87.7 87.7 86.2 98.5 89.6 87.7 11 D04-14781(1-5) Black Jack Pork
12 1.2 0.8 0.8 10.7 1.2 16.4 5.6 1.3 1.0 1.0 10.5 89.1 89.7 99.3 98.8 98.8 93.2 88.1 85.7 93.9 87.1 85.7 89.1 89.9 88.9 89.1 89.4 89.4 89.2 94.4 99.0 99.3 99.3 89.9 90.0 90.0 89.9 91.9 91.9 90.2 91.5 91.9 91.9 95.5 89.4 85.2 87.1 85.7 89.4 99.2 87.1 12 D04-21622 Future Pork
13 11.4 11.1 11.0 2.0 10.6 16.4 14.8 10.9 10.9 10.9 1.7 10.9 98.5 89.1 89.6 89.7 85.4 97.3 88.9 86.4 95.4 88.9 98.7 87.2 87.4 98.7 99.0 99.0 98.8 86.9 88.7 89.6 89.2 87.1 87.2 87.2 87.1 88.7 88.7 87.4 88.4 88.7 88.7 87.2 87.1 87.4 86.4 84.9 98.2 89.1 86.4 13 D04-17554(1-2) K&K BlackJack Pork
14 10.9 10.5 10.5 1.5 10.1 16.6 14.1 10.3 10.3 9.9 0.2 10.3 1.5 89.7 90.2 89.7 85.4 98.0 89.6 86.7 96.4 89.6 99.2 87.9 87.6 99.2 99.5 99.5 99.3 87.1 89.4 90.2 89.9 87.4 87.6 87.6 87.7 89.1 89.1 88.1 88.7 89.1 89.1 87.4 87.6 87.6 87.6 86.1 98.7 89.7 87.6 14 D04-16905(1-3) Walbexer Black Jack
15 1.2 0.8 0.8 10.7 1.2 16.8 5.9 1.3 1.0 1.0 10.5 0.7 10.9 10.3 98.8 98.8 92.7 88.1 85.6 93.9 87.1 85.6 89.1 89.4 88.4 89.1 89.4 89.4 89.2 94.4 99.0 99.0 99.3 89.6 89.7 89.7 89.4 91.5 91.5 89.7 91.2 91.5 91.5 95.5 89.1 85.2 86.9 85.6 89.4 99.2 86.9 15 D04-16408-1 Illini Swine
16 1.2 0.8 0.8 10.4 0.5 17.0 5.6 0.7 0.8 0.7 10.3 1.2 10.6 10.1 1.2 98.8 93.4 88.6 86.1 94.7 87.6 86.1 89.6 90.0 89.1 89.6 89.9 89.9 89.7 95.2 99.3 99.0 99.3 89.7 89.9 89.9 90.0 92.0 92.0 90.4 91.7 92.0 92.0 96.4 89.6 85.6 87.1 85.7 89.9 99.2 87.1 16 D04-33869 County Line Pork
17 1.0 0.7 0.7 10.5 1.0 16.6 5.4 1.2 0.8 0.5 10.3 1.2 10.7 10.1 1.2 1.2 93.2 88.2 86.7 94.0 87.2 86.7 89.7 90.2 89.2 89.7 90.0 90.0 89.9 94.5 99.2 99.2 99.5 89.9 90.0 90.0 90.2 91.9 91.9 90.5 91.5 91.9 91.9 95.7 89.4 85.4 87.1 85.7 90.0 99.3 87.1 17 D05-8868(4-6) County Line
18 6.9 6.5 6.3 15.2 6.3 17.4 2.2 6.5 6.7 6.5 15.0 6.9 15.0 14.8 7.2 6.5 6.7 84.2 82.1 96.7 82.8 82.1 85.1 86.7 86.2 84.7 85.2 85.2 85.1 97.2 93.2 93.0 93.4 86.4 86.6 86.6 86.6 87.9 87.9 86.9 87.7 87.9 87.9 96.5 86.2 81.8 84.2 82.8 85.2 93.4 84.2 18 D05-46346(7-9) S&S Farms
19 11.0 10.6 10.6 1.0 10.2 14.8 14.5 10.4 10.4 10.4 0.8 10.4 1.4 0.7 10.4 10.2 10.4 14.7 88.9 85.4 95.4 88.9 98.0 86.6 87.2 98.0 98.3 98.3 98.2 85.9 88.1 88.6 88.2 86.6 86.7 86.7 86.4 88.4 88.4 86.7 88.1 88.4 88.4 86.2 86.9 87.4 87.1 85.6 98.0 88.1 87.1 19 D03-18981(1,2,4,5) Black Jack Pork
20 14.1 13.7 13.7 11.5 13.7 17.2 17.8 13.9 13.5 13.7 10.9 13.9 12.0 11.2 13.9 13.7 13.1 18.2 10.3 83.3 88.7 100.0 89.4 86.6 85.9 89.4 89.7 89.7 89.9 83.4 86.2 85.9 86.2 86.9 87.2 87.1 86.4 87.4 87.4 86.6 87.2 87.4 87.4 83.7 85.7 87.2 84.2 82.8 89.6 86.1 84.2 20 D03-18980(5,8) Black Jack Pork
21 5.4 5.0 4.8 13.9 4.8 17.1 1.9 5.0 5.2 5.0 13.7 5.7 13.9 13.5 5.7 5.0 5.6 3.2 13.5 17.4 84.1 83.3 86.2 87.7 86.9 85.9 86.4 86.4 86.2 99.3 94.7 94.2 94.5 87.4 87.6 87.6 87.6 88.9 88.9 87.9 88.7 88.9 88.9 98.5 87.6 82.9 84.6 83.1 86.4 94.5 84.6 21 D03-19304(20-23) S&S Farms
22 9.9 9.6 9.5 1.6 9.1 14.8 13.7 9.4 9.4 9.4 0.7 9.4 1.4 0.5 9.4 9.1 9.4 13.9 0.7 9.0 12.6 88.7 96.0 85.7 86.6 95.9 96.4 96.4 96.2 84.4 86.7 87.6 87.2 85.6 85.7 85.7 85.6 87.6 87.6 85.9 87.6 87.6 87.6 84.7 86.2 85.4 85.1 83.3 95.4 87.1 85.1 22 D03-16034(1-7-9) Black Jack Pork
23 14.1 13.7 13.7 11.5 13.7 17.2 17.8 13.9 13.5 13.7 10.9 13.9 12.0 11.2 13.9 13.7 13.1 18.2 10.3 0.0 17.4 9.0 89.4 86.6 85.9 89.4 89.7 89.7 89.9 83.4 86.2 85.9 86.2 86.9 87.2 87.1 86.4 87.4 87.4 86.6 87.2 87.4 87.4 83.7 85.7 87.2 84.2 82.8 89.6 86.1 84.2 23 D03-16035(5-8) Black Jack Pork
24 11.5 11.1 11.1 1.3 10.7 15.5 15.0 10.9 10.9 10.9 1.0 10.9 1.3 0.8 10.9 10.7 10.7 15.2 0.7 11.6 13.9 0.9 11.6 87.7 87.7 99.3 99.7 99.7 99.5 86.7 88.7 89.6 89.2 87.6 87.7 87.7 87.6 89.2 89.2 87.9 88.9 89.2 89.2 87.1 87.7 87.7 87.4 85.9 98.8 89.1 87.4 24 D03-14810-23 Black Jack Pork
25 10.9 10.5 10.5 12.8 10.1 17.7 13.2 10.3 10.5 9.9 12.6 10.3 13.4 12.4 10.7 10.1 10.1 14.0 12.5 14.6 12.9 11.5 14.6 12.8 95.7 87.6 87.9 87.9 87.7 87.9 89.9 89.9 89.9 95.5 95.7 95.7 99.3 97.8 97.8 99.5 97.7 97.8 97.8 88.2 96.0 87.2 85.4 84.1 87.9 89.7 85.4 25 D03-13137(11-15) Illini Farm 1904
26 11.3 10.9 10.9 11.7 10.5 16.5 14.0 10.7 10.7 10.7 11.9 10.7 12.3 11.7 11.1 10.5 10.5 14.2 11.2 14.0 13.3 10.0 14.0 11.7 3.6 87.6 88.1 88.1 87.9 87.1 88.9 88.9 88.9 94.2 94.4 94.4 95.7 96.7 96.7 95.9 96.8 96.7 96.7 87.6 94.5 86.2 84.7 83.1 88.1 88.7 84.7 26 D03-10907-3 LincolnLand Farms
27 11.4 11.1 11.0 1.3 10.6 16.4 15.2 10.9 10.9 10.9 1.0 10.9 1.3 0.8 10.9 10.6 10.7 15.4 0.7 11.5 14.1 0.9 11.5 0.7 13.0 11.9 99.7 99.7 99.5 86.4 88.7 89.6 89.2 87.4 87.2 87.4 87.4 89.1 89.1 87.7 88.7 89.1 89.1 86.7 87.4 87.9 86.9 85.7 98.8 89.1 86.9 27 D03-10033 Black Jack Pork
28 11.1 10.7 10.7 1.0 10.3 15.9 14.8 10.5 10.5 10.5 0.7 10.5 1.0 0.5 10.5 10.3 10.3 15.0 0.3 11.2 13.7 0.5 11.2 0.3 12.6 11.5 0.3 100.0 99.8 86.9 89.1 89.9 89.6 87.7 87.9 87.9 87.7 89.4 89.4 88.1 89.1 89.4 89.4 87.2 87.9 88.1 87.2 85.7 99.2 89.4 87.2 28 D03-10529(1-4) Black Jack Pork
29 11.1 10.7 10.7 1.0 10.3 15.9 14.8 10.5 10.5 10.5 0.7 10.5 1.0 0.5 10.5 10.3 10.3 15.0 0.3 11.2 13.7 0.5 11.2 0.3 12.6 11.5 0.3 0.0 99.8 86.9 89.1 89.9 89.6 87.7 87.9 87.9 87.7 89.4 89.4 88.1 89.1 89.4 89.4 87.2 87.9 88.1 87.2 85.7 99.2 89.4 87.2 29 D03-10935(1-4) Black Jack Pork
30 11.3 10.9 10.9 1.2 10.5 16.2 15.0 10.7 10.7 10.7 0.8 10.7 1.2 0.7 10.7 10.5 10.5 15.2 0.5 10.9 13.9 0.7 10.9 0.5 12.8 11.7 0.5 0.2 0.2 86.7 88.9 89.7 89.4 87.9 88.1 88.1 87.6 89.2 89.2 87.9 88.9 89.2 89.2 87.1 87.7 87.9 87.1 85.6 99.0 89.2 87.1 30 D03-10530-1 Black Jack Pork
31 4.8 4.5 4.3 13.5 4.3 17.4 1.3 4.5 4.7 4.5 13.3 5.2 13.5 13.1 5.2 4.5 5.0 2.7 13.1 16.9 0.7 12.2 16.9 13.5 12.8 13.1 13.7 13.3 13.3 13.5 95.2 94.7 95.0 87.6 87.7 87.7 87.7 89.1 89.1 88.1 88.9 89.1 89.1 99.0 87.7 83.4 85.1 83.6 86.9 95.0 85.1 31 D03-6293-4 S&S Farms (102850)
32 0.8 0.5 0.5 11.1 0.8 16.8 5.2 1.0 0.7 0.7 10.9 1.0 11.3 10.7 1.0 0.7 0.8 6.5 10.6 13.5 5.0 9.8 13.5 11.3 10.3 10.7 11.3 10.9 10.9 11.1 4.5 99.3 99.7 89.9 90.0 90.0 89.9 91.9 91.9 90.2 91.5 91.9 91.9 96.2 89.6 85.2 87.2 85.9 89.1 99.5 87.2 32 D02-48860(4-8) Crescent Pork
33 0.8 0.5 0.5 10.3 0.8 16.4 5.6 1.0 0.7 0.7 10.1 0.7 10.5 9.9 1.0 1.0 0.8 6.9 10.0 13.9 5.4 9.0 13.9 10.5 10.3 10.7 10.5 10.1 10.1 10.3 4.8 0.7 99.7 89.9 90.0 90.0 89.9 91.9 91.9 90.2 91.5 91.9 91.9 95.9 89.4 84.7 87.2 85.9 89.9 99.5 87.2 33 D02-45742-1 Black Jack Pork
34 0.5 0.2 0.2 10.7 0.5 16.8 5.2 0.7 0.3 0.3 10.5 0.7 10.9 10.3 0.7 0.7 0.5 6.5 10.4 13.5 5.0 9.4 13.5 10.9 10.3 10.7 10.9 10.5 10.5 10.7 4.5 0.3 0.3 89.9 90.0 90.0 89.9 91.9 91.9 90.2 91.5 91.9 91.9 96.2 89.4 85.1 87.2 85.9 89.6 99.8 87.2 34 D02-45742-2 Black Jack Pork
35 10.9 10.5 10.5 12.7 10.5 17.7 13.8 10.8 10.6 10.7 13.0 10.3 13.4 12.8 10.7 10.5 10.5 14.5 12.3 13.6 13.2 11.7 13.6 12.8 4.5 4.8 13.0 12.5 12.5 12.3 13.0 10.3 10.3 10.3 99.5 99.7 95.5 96.8 96.8 95.7 96.7 96.8 96.8 87.9 94.5 86.2 84.4 82.9 87.7 89.7 84.4 35 D04-49972-1 Illini Swine Inc
36 10.9 10.5 10.5 12.3 10.5 17.6 13.8 10.7 10.5 10.7 12.9 10.3 13.3 12.7 10.7 10.5 10.5 14.4 12.3 13.5 13.1 11.6 13.5 12.7 4.5 4.8 12.9 12.5 12.5 12.3 12.9 10.3 10.3 10.3 0.3 99.7 95.7 97.0 97.0 95.9 96.8 97.0 97.0 88.1 94.7 86.4 84.9 83.3 87.9 89.9 84.9 36 D04-49972-2 Illini Swine Inc
37 10.9 10.5 10.5 12.7 10.5 17.6 13.8 10.7 10.5 10.7 12.9 10.3 13.3 12.7 10.7 10.5 10.5 14.5 12.3 13.5 13.2 11.6 13.5 12.7 4.5 4.8 12.9 12.5 12.5 12.3 13.0 10.3 10.3 10.3 0.2 0.3 95.7 97.0 97.0 95.9 96.8 97.0 97.0 88.1 94.7 86.4 84.6 82.9 87.9 89.9 84.6 37 D04-49972-3 Illini Swine Inc
38 10.9 10.5 10.5 12.9 10.1 17.9 13.2 10.4 10.6 9.9 12.8 10.3 13.6 12.5 10.7 10.1 10.1 14.3 12.7 14.8 13.0 11.6 14.8 13.0 0.7 3.6 13.2 12.8 12.8 13.0 12.8 10.3 10.3 10.3 4.5 4.5 4.5 97.8 97.8 99.5 97.7 97.8 97.8 88.1 95.2 86.9 85.2 84.1 87.7 89.7 85.2 38 D03-6295-3 Illini-North
39 9.1 8.8 8.8 11.1 8.4 16.8 12.6 8.6 8.8 8.6 11.3 8.6 11.7 11.1 9.0 8.4 8.8 13.2 10.8 13.1 11.9 9.8 13.1 11.1 2.2 2.7 11.3 10.9 10.9 11.1 11.7 8.6 8.6 8.6 2.9 2.9 2.9 2.2 100.0 98.0 99.8 100.0 100.0 89.7 96.2 88.1 86.2 84.7 89.4 91.7 86.2 39 D03-5293(2-4) I llini Swine-Hich Tec
40 9.1 8.8 8.8 11.1 8.4 16.8 12.6 8.6 8.8 8.6 11.3 8.6 11.7 11.1 9.0 8.4 8.8 13.2 10.8 13.1 11.9 9.8 13.1 11.1 2.2 2.7 11.3 10.9 10.9 11.1 11.7 8.6 8.6 8.6 2.9 2.9 2.9 2.2 0.0 98.0 99.8 100.0 100.0 89.7 96.2 88.1 86.2 84.7 89.4 91.7 86.2 40 D03-5293(5-7) I llini Swine-High Tec
41 10.3 10.0 9.9 12.3 9.5 17.5 12.6 9.8 10.0 9.4 12.2 9.8 13.0 11.9 10.2 9.5 9.6 13.6 12.1 14.4 12.4 11.0 14.4 12.4 0.3 3.2 12.5 12.2 12.2 12.4 12.2 9.8 9.8 9.8 4.1 4.1 4.1 0.3 1.9 1.9 97.8 98.0 98.0 88.4 95.7 87.4 85.6 84.2 88.1 90.0 85.6 41 D03-5296(3-4) I llini Swine-North
42 9.3 9.0 8.9 11.3 8.6 16.6 12.8 8.8 9.0 8.8 11.5 8.8 11.9 11.3 9.2 8.6 9.0 13.4 11.1 13.3 12.1 9.8 13.3 11.3 2.4 2.5 11.5 11.1 11.1 11.3 12.0 8.8 8.8 8.8 3.1 3.1 3.1 2.4 0.2 0.2 2.0 99.8 99.8 89.6 96.0 87.7 85.9 84.4 89.1 91.4 85.9 42 D02-61015-3 Illini Farms South
43 9.1 8.8 8.8 11.1 8.4 16.8 12.6 8.6 8.8 8.6 11.3 8.6 11.7 11.1 9.0 8.4 8.8 13.2 10.8 13.1 11.9 9.8 13.1 11.1 2.2 2.7 11.3 10.9 10.9 11.1 11.7 8.6 8.6 8.6 2.9 2.9 2.9 2.2 0.0 0.0 1.9 0.2 100.0 89.7 96.2 88.1 86.2 84.7 89.4 91.7 86.2 43 D02-61016-1 Illini Farms South
44 9.1 8.8 8.8 11.1 8.4 16.8 12.6 8.6 8.8 8.6 11.3 8.6 11.7 11.1 9.0 8.4 8.8 13.2 10.8 13.1 11.9 9.8 13.1 11.1 2.2 2.7 11.3 10.9 10.9 11.1 11.7 8.6 8.6 8.6 2.9 2.9 2.9 2.2 0.0 0.0 1.9 0.2 0.0 89.7 96.2 88.1 86.2 84.7 89.4 91.7 86.2 44 D02-48857(5-8) I llini South
45 4.1 3.8 3.6 13.5 3.6 16.9 2.2 3.8 4.0 3.8 13.3 4.5 13.5 13.1 4.5 3.8 4.3 3.4 13.0 16.9 1.5 12.2 16.9 13.5 12.5 12.7 13.7 13.3 13.3 13.5 1.0 3.8 4.1 3.8 12.8 12.7 12.8 12.6 11.1 11.1 12.0 11.3 11.1 11.1 87.9 83.6 85.6 84.1 87.2 96.2 85.6 45 D02-48858(7-9) S&S Farms
46 10.9 10.9 10.9 12.2 10.5 18.0 13.4 10.8 11.0 10.7 12.8 10.7 13.4 12.6 11.1 10.5 10.9 14.3 12.1 15.3 13.0 10.9 15.3 12.6 3.8 4.5 12.8 12.4 12.4 12.6 12.8 10.7 10.7 10.7 5.2 5.2 5.2 4.5 3.6 3.6 4.0 3.8 3.6 3.6 12.8 87.9 85.6 83.9 87.9 89.2 85.6 46 D02-47395(1-2) Nipco Farms
47 14.5 14.5 14.5 12.3 14.1 18.5 17.5 13.7 14.6 14.3 13.2 14.3 13.6 13.4 14.3 14.1 14.3 18.0 12.1 13.2 16.9 12.5 13.2 13.2 13.5 14.1 12.9 12.8 12.8 13.0 16.5 14.3 14.7 14.3 14.4 14.4 14.4 13.7 12.7 12.7 13.1 12.9 12.7 12.7 16.9 12.3 84.2 83.1 88.1 84.9 84.2 47 D02-47394(5-8) Prime Pork
48 13.5 13.3 13.3 13.3 13.1 12.8 15.6 13.1 12.9 13.1 12.5 13.1 13.7 12.7 13.1 13.3 12.9 16.5 12.0 16.4 16.2 12.4 16.4 12.9 14.1 14.3 13.3 12.9 12.9 13.1 15.6 13.1 13.1 13.1 15.2 15.2 15.2 14.3 13.7 13.7 13.7 13.9 13.7 13.7 15.6 14.2 16.5 98.7 87.2 87.1 100.0 48 D06-28296-5 Boars
49 13.9 13.7 13.7 13.7 13.5 13.2 16.0 13.5 13.3 13.5 12.8 13.5 14.1 13.1 13.5 13.7 13.3 16.9 12.4 16.6 16.7 12.8 16.6 13.3 14.5 14.7 13.3 13.3 13.3 13.5 16.0 13.5 13.5 13.5 15.7 15.6 15.6 14.5 14.1 14.1 14.1 14.3 14.1 14.1 16.0 14.6 16.7 0.2 85.7 85.7 98.7 49 D06-26717-1 Boars
50 11.0 10.7 10.6 0.5 10.2 15.7 14.7 10.5 10.5 10.5 1.5 10.5 1.9 1.3 10.5 10.2 10.3 15.0 0.8 11.3 13.7 1.4 11.3 1.2 12.5 11.5 1.2 0.8 0.8 1.0 13.3 10.9 10.1 10.5 12.5 12.5 12.5 12.7 10.9 10.9 12.1 11.1 10.9 10.9 13.3 12.4 12.5 13.1 13.5 89.4 87.2 50 IngelvacATP
51 0.3 0.0 0.2 10.9 0.7 17.0 5.2 0.8 0.5 0.5 10.7 0.8 11.1 10.5 0.8 0.8 0.7 6.5 10.6 13.7 5.0 9.6 13.7 11.1 10.5 10.9 11.1 10.7 10.7 10.9 4.5 0.5 0.5 0.2 10.5 10.5 10.5 10.5 8.8 8.8 10.0 9.0 8.8 8.8 3.8 10.9 14.5 13.3 13.7 10.7 87.1 51 RespPRRS
52 13.5 13.3 13.3 13.3 13.1 12.8 15.6 13.1 12.9 13.1 12.5 13.1 13.7 12.7 13.1 13.3 12.9 16.5 12.0 16.4 16.2 12.4 16.4 12.9 14.1 14.3 13.3 12.9 12.9 13.1 15.6 13.1 13.1 13.1 15.2 15.2 15.2 14.3 13.7 13.7 13.7 13.9 13.7 13.7 15.6 14.2 16.5 0.0 0.2 13.1 13.3 52 D06-30617-1 FuturePork
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Percent Identity
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
1 99.7 99.5 88.7 99.3 82.8 94.2 98.8 99.2 99.2 89.2 98.8 88.7 89.4 98.8 98.8 99.0 92.9 87.7 85.7 94.2 86.7 85.7 88.7 89.4 88.4 88.7 89.1 89.1 88.9 94.7 99.2 99.2 99.5 89.4 89.6 89.6 89.4 91.4 91.4 89.7 91.0 91.4 91.4 95.9 89.2 84.9 86.9 85.6 89.1 99.7 86.9 1 D04-33870 Black Jack/Holtkai
2 0.3 99.8 89.1 99.3 83.3 94.5 99.0 99.2 99.5 89.6 99.2 89.1 89.7 99.2 99.2 99.3 93.4 88.1 86.1 94.5 87.1 86.1 89.1 89.7 88.7 89.1 89.4 89.4 89.2 95.0 99.5 99.5 99.8 89.7 89.9 89.9 89.7 91.7 91.7 90.0 91.4 91.7 91.7 96.2 89.2 84.9 87.1 85.7 89.4 100.0 87.1 2 D04-29274 Black Jack Pork
3 0.5 0.2 89.1 99.3 83.3 94.7 99.0 99.2 99.5 89.6 99.2 89.1 89.7 99.2 99.2 99.3 93.5 88.1 86.1 94.7 87.1 86.1 89.1 89.7 88.7 89.1 89.4 89.4 89.2 95.2 99.5 99.5 99.8 89.7 89.9 89.9 89.7 91.7 91.7 90.0 91.4 91.7 91.7 96.4 89.2 84.9 87.1 85.7 89.4 99.8 87.1 3 D04-49976 Weidner, Al
4 11.2 10.9 10.8 89.6 84.6 85.1 89.2 88.9 89.2 98.3 89.1 98.0 98.5 89.1 89.6 89.7 84.9 97.8 89.4 86.1 95.2 89.4 98.7 87.6 87.7 98.5 99.0 99.0 98.8 86.6 88.7 89.6 89.2 87.4 87.9 87.6 87.2 89.1 89.1 87.7 88.7 89.1 89.1 86.9 88.2 88.1 87.1 85.6 99.5 89.1 87.1 4 D06-21801(4-6) H&P
5 0.7 0.7 0.7 10.4 83.1 94.2 99.5 99.3 99.5 90.0 98.8 89.6 90.2 98.8 99.5 99.0 93.7 88.6 86.1 94.9 87.6 86.1 89.6 90.0 89.1 89.6 89.9 89.9 89.7 95.4 99.2 99.2 99.5 89.7 89.9 89.9 90.0 92.0 92.0 90.4 91.7 92.0 92.0 96.5 89.6 85.6 87.2 85.9 89.9 99.3 87.2 5 D06-16814(21-25) County Line Pork
6 17.5 17.0 17.0 15.9 17.0 81.9 82.8 82.9 82.9 84.2 83.6 84.1 84.1 83.3 83.1 83.7 82.4 84.4 83.4 82.6 82.8 83.4 85.1 83.3 83.1 84.1 84.6 84.6 84.4 82.4 83.3 83.7 83.4 82.8 83.1 82.9 83.1 83.7 83.7 83.3 83.9 83.7 83.7 83.3 82.8 82.6 87.9 86.2 84.6 83.3 87.9 6 D06-13563-22 Sand Ridge
7 5.6 5.2 5.0 15.0 5.4 17.8 93.9 93.5 94.9 85.7 94.2 85.6 85.9 93.9 94.0 94.4 97.8 84.4 82.6 98.2 82.9 82.6 85.2 87.4 86.4 84.9 85.4 85.4 85.2 98.7 94.7 94.2 94.5 86.7 86.9 86.9 87.2 88.2 88.2 87.6 88.1 88.2 88.2 97.8 87.1 82.4 84.9 83.4 85.4 94.5 84.9 7 D06-6000-1 Huftalin/Dreska
8 1.0 0.8 0.8 10.7 0.3 17.3 5.6 98.8 99.2 89.7 98.5 89.2 89.9 98.5 99.2 98.7 93.4 88.2 85.6 94.5 87.2 85.6 89.2 89.7 88.7 89.2 89.6 89.6 89.4 95.0 98.8 98.8 99.2 89.4 89.6 89.6 89.7 91.7 91.7 90.0 91.4 91.7 91.7 96.2 89.2 85.7 87.1 85.7 89.6 99.0 87.1 8 D06-3518(1-5) County Line Pork
9 0.5 0.5 0.5 10.7 0.3 16.8 5.6 0.7 99.0 89.4 98.7 88.9 89.6 98.7 98.8 98.8 92.7 87.9 85.9 93.9 86.9 85.9 88.9 89.4 88.6 88.9 89.2 89.2 89.1 94.4 99.0 99.0 99.3 89.2 89.4 89.4 89.4 91.4 91.4 89.7 91.0 91.4 91.4 95.7 88.7 84.2 87.1 85.7 89.2 99.2 87.1 9 D05-65768(1-5) County Line Pork
10 0.8 0.5 0.5 10.7 0.5 17.3 4.8 0.7 0.7 90.0 99.0 89.2 90.2 99.0 99.3 99.5 93.4 88.2 86.1 94.5 87.2 86.1 89.2 90.2 88.9 89.2 89.6 89.6 89.4 95.0 99.3 99.3 99.7 89.6 89.7 89.7 90.2 91.9 91.9 90.5 91.5 91.9 91.9 96.2 89.4 85.2 87.2 85.9 89.6 99.5 87.2 10 D05-56088(16-20) County Line Pork
11 11.1 10.7 10.7 1.7 10.3 16.4 14.3 10.5 10.5 10.1 89.6 98.3 99.8 89.6 90.0 89.6 85.2 97.8 89.7 86.6 96.2 89.7 99.0 87.7 87.4 99.0 99.3 99.3 99.2 86.9 89.2 90.0 89.7 87.1 87.4 87.2 87.6 88.9 88.9 87.9 88.6 88.9 88.9 87.2 87.4 87.7 87.7 86.2 98.5 89.6 87.7 11 D04-14781(1-5) Black Jack Pork
12 1.2 0.8 0.8 10.7 1.2 16.4 5.6 1.3 1.0 1.0 10.5 89.1 89.7 99.3 98.8 98.8 93.2 88.1 85.7 93.9 87.1 85.7 89.1 89.9 88.9 89.1 89.4 89.4 89.2 94.4 99.0 99.3 99.3 89.9 90.0 90.0 89.9 91.9 91.9 90.2 91.5 91.9 91.9 95.5 89.4 85.2 87.1 85.7 89.4 99.2 87.1 12 D04-21622 Future Pork
13 11.4 11.1 11.0 2.0 10.6 16.4 14.8 10.9 10.9 10.9 1.7 10.9 98.5 89.1 89.6 89.7 85.4 97.3 88.9 86.4 95.4 88.9 98.7 87.2 87.4 98.7 99.0 99.0 98.8 86.9 88.7 89.6 89.2 87.1 87.2 87.2 87.1 88.7 88.7 87.4 88.4 88.7 88.7 87.2 87.1 87.4 86.4 84.9 98.2 89.1 86.4 13 D04-17554(1-2) K&K BlackJack Pork
14 10.9 10.5 10.5 1.5 10.1 16.6 14.1 10.3 10.3 9.9 0.2 10.3 1.5 89.7 90.2 89.7 85.4 98.0 89.6 86.7 96.4 89.6 99.2 87.9 87.6 99.2 99.5 99.5 99.3 87.1 89.4 90.2 89.9 87.4 87.6 87.6 87.7 89.1 89.1 88.1 88.7 89.1 89.1 87.4 87.6 87.6 87.6 86.1 98.7 89.7 87.6 14 D04-16905(1-3) Walbexer Black Jack
15 1.2 0.8 0.8 10.7 1.2 16.8 5.9 1.3 1.0 1.0 10.5 0.7 10.9 10.3 98.8 98.8 92.7 88.1 85.6 93.9 87.1 85.6 89.1 89.4 88.4 89.1 89.4 89.4 89.2 94.4 99.0 99.0 99.3 89.6 89.7 89.7 89.4 91.5 91.5 89.7 91.2 91.5 91.5 95.5 89.1 85.2 86.9 85.6 89.4 99.2 86.9 15 D04-16408-1 Illini Swine
16 1.2 0.8 0.8 10.4 0.5 17.0 5.6 0.7 0.8 0.7 10.3 1.2 10.6 10.1 1.2 98.8 93.4 88.6 86.1 94.7 87.6 86.1 89.6 90.0 89.1 89.6 89.9 89.9 89.7 95.2 99.3 99.0 99.3 89.7 89.9 89.9 90.0 92.0 92.0 90.4 91.7 92.0 92.0 96.4 89.6 85.6 87.1 85.7 89.9 99.2 87.1 16 D04-33869 County Line Pork
17 1.0 0.7 0.7 10.5 1.0 16.6 5.4 1.2 0.8 0.5 10.3 1.2 10.7 10.1 1.2 1.2 93.2 88.2 86.7 94.0 87.2 86.7 89.7 90.2 89.2 89.7 90.0 90.0 89.9 94.5 99.2 99.2 99.5 89.9 90.0 90.0 90.2 91.9 91.9 90.5 91.5 91.9 91.9 95.7 89.4 85.4 87.1 85.7 90.0 99.3 87.1 17 D05-8868(4-6) County Line
18 6.9 6.5 6.3 15.2 6.3 17.4 2.2 6.5 6.7 6.5 15.0 6.9 15.0 14.8 7.2 6.5 6.7 84.2 82.1 96.7 82.8 82.1 85.1 86.7 86.2 84.7 85.2 85.2 85.1 97.2 93.2 93.0 93.4 86.4 86.6 86.6 86.6 87.9 87.9 86.9 87.7 87.9 87.9 96.5 86.2 81.8 84.2 82.8 85.2 93.4 84.2 18 D05-46346(7-9) S&S Farms
19 11.0 10.6 10.6 1.0 10.2 14.8 14.5 10.4 10.4 10.4 0.8 10.4 1.4 0.7 10.4 10.2 10.4 14.7 88.9 85.4 95.4 88.9 98.0 86.6 87.2 98.0 98.3 98.3 98.2 85.9 88.1 88.6 88.2 86.6 86.7 86.7 86.4 88.4 88.4 86.7 88.1 88.4 88.4 86.2 86.9 87.4 87.1 85.6 98.0 88.1 87.1 19 D03-18981(1,2,4,5) Black Jack Pork
20 14.1 13.7 13.7 11.5 13.7 17.2 17.8 13.9 13.5 13.7 10.9 13.9 12.0 11.2 13.9 13.7 13.1 18.2 10.3 83.3 88.7 100.0 89.4 86.6 85.9 89.4 89.7 89.7 89.9 83.4 86.2 85.9 86.2 86.9 87.2 87.1 86.4 87.4 87.4 86.6 87.2 87.4 87.4 83.7 85.7 87.2 84.2 82.8 89.6 86.1 84.2 20 D03-18980(5,8) Black Jack Pork
21 5.4 5.0 4.8 13.9 4.8 17.1 1.9 5.0 5.2 5.0 13.7 5.7 13.9 13.5 5.7 5.0 5.6 3.2 13.5 17.4 84.1 83.3 86.2 87.7 86.9 85.9 86.4 86.4 86.2 99.3 94.7 94.2 94.5 87.4 87.6 87.6 87.6 88.9 88.9 87.9 88.7 88.9 88.9 98.5 87.6 82.9 84.6 83.1 86.4 94.5 84.6 21 D03-19304(20-23) S&S Farms
22 9.9 9.6 9.5 1.6 9.1 14.8 13.7 9.4 9.4 9.4 0.7 9.4 1.4 0.5 9.4 9.1 9.4 13.9 0.7 9.0 12.6 88.7 96.0 85.7 86.6 95.9 96.4 96.4 96.2 84.4 86.7 87.6 87.2 85.6 85.7 85.7 85.6 87.6 87.6 85.9 87.6 87.6 87.6 84.7 86.2 85.4 85.1 83.3 95.4 87.1 85.1 22 D03-16034(1-7-9) Black Jack Pork
23 14.1 13.7 13.7 11.5 13.7 17.2 17.8 13.9 13.5 13.7 10.9 13.9 12.0 11.2 13.9 13.7 13.1 18.2 10.3 0.0 17.4 9.0 89.4 86.6 85.9 89.4 89.7 89.7 89.9 83.4 86.2 85.9 86.2 86.9 87.2 87.1 86.4 87.4 87.4 86.6 87.2 87.4 87.4 83.7 85.7 87.2 84.2 82.8 89.6 86.1 84.2 23 D03-16035(5-8) Black Jack Pork
24 11.5 11.1 11.1 1.3 10.7 15.5 15.0 10.9 10.9 10.9 1.0 10.9 1.3 0.8 10.9 10.7 10.7 15.2 0.7 11.6 13.9 0.9 11.6 87.7 87.7 99.3 99.7 99.7 99.5 86.7 88.7 89.6 89.2 87.6 87.7 87.7 87.6 89.2 89.2 87.9 88.9 89.2 89.2 87.1 87.7 87.7 87.4 85.9 98.8 89.1 87.4 24 D03-14810-23 Black Jack Pork
25 10.9 10.5 10.5 12.8 10.1 17.7 13.2 10.3 10.5 9.9 12.6 10.3 13.4 12.4 10.7 10.1 10.1 14.0 12.5 14.6 12.9 11.5 14.6 12.8 95.7 87.6 87.9 87.9 87.7 87.9 89.9 89.9 89.9 95.5 95.7 95.7 99.3 97.8 97.8 99.5 97.7 97.8 97.8 88.2 96.0 87.2 85.4 84.1 87.9 89.7 85.4 25 D03-13137(11-15) Illini Farm 1904
26 11.3 10.9 10.9 11.7 10.5 16.5 14.0 10.7 10.7 10.7 11.9 10.7 12.3 11.7 11.1 10.5 10.5 14.2 11.2 14.0 13.3 10.0 14.0 11.7 3.6 87.6 88.1 88.1 87.9 87.1 88.9 88.9 88.9 94.2 94.4 94.4 95.7 96.7 96.7 95.9 96.8 96.7 96.7 87.6 94.5 86.2 84.7 83.1 88.1 88.7 84.7 26 D03-10907-3 LincolnLand Farms
27 11.4 11.1 11.0 1.3 10.6 16.4 15.2 10.9 10.9 10.9 1.0 10.9 1.3 0.8 10.9 10.6 10.7 15.4 0.7 11.5 14.1 0.9 11.5 0.7 13.0 11.9 99.7 99.7 99.5 86.4 88.7 89.6 89.2 87.4 87.2 87.4 87.4 89.1 89.1 87.7 88.7 89.1 89.1 86.7 87.4 87.9 86.9 85.7 98.8 89.1 86.9 27 D03-10033 Black Jack Pork
28 11.1 10.7 10.7 1.0 10.3 15.9 14.8 10.5 10.5 10.5 0.7 10.5 1.0 0.5 10.5 10.3 10.3 15.0 0.3 11.2 13.7 0.5 11.2 0.3 12.6 11.5 0.3 100.0 99.8 86.9 89.1 89.9 89.6 87.7 87.9 87.9 87.7 89.4 89.4 88.1 89.1 89.4 89.4 87.2 87.9 88.1 87.2 85.7 99.2 89.4 87.2 28 D03-10529(1-4) Black Jack Pork
29 11.1 10.7 10.7 1.0 10.3 15.9 14.8 10.5 10.5 10.5 0.7 10.5 1.0 0.5 10.5 10.3 10.3 15.0 0.3 11.2 13.7 0.5 11.2 0.3 12.6 11.5 0.3 0.0 99.8 86.9 89.1 89.9 89.6 87.7 87.9 87.9 87.7 89.4 89.4 88.1 89.1 89.4 89.4 87.2 87.9 88.1 87.2 85.7 99.2 89.4 87.2 29 D03-10935(1-4) Black Jack Pork
30 11.3 10.9 10.9 1.2 10.5 16.2 15.0 10.7 10.7 10.7 0.8 10.7 1.2 0.7 10.7 10.5 10.5 15.2 0.5 10.9 13.9 0.7 10.9 0.5 12.8 11.7 0.5 0.2 0.2 86.7 88.9 89.7 89.4 87.9 88.1 88.1 87.6 89.2 89.2 87.9 88.9 89.2 89.2 87.1 87.7 87.9 87.1 85.6 99.0 89.2 87.1 30 D03-10530-1 Black Jack Pork
31 4.8 4.5 4.3 13.5 4.3 17.4 1.3 4.5 4.7 4.5 13.3 5.2 13.5 13.1 5.2 4.5 5.0 2.7 13.1 16.9 0.7 12.2 16.9 13.5 12.8 13.1 13.7 13.3 13.3 13.5 95.2 94.7 95.0 87.6 87.7 87.7 87.7 89.1 89.1 88.1 88.9 89.1 89.1 99.0 87.7 83.4 85.1 83.6 86.9 95.0 85.1 31 D03-6293-4 S&S Farms (102850)
32 0.8 0.5 0.5 11.1 0.8 16.8 5.2 1.0 0.7 0.7 10.9 1.0 11.3 10.7 1.0 0.7 0.8 6.5 10.6 13.5 5.0 9.8 13.5 11.3 10.3 10.7 11.3 10.9 10.9 11.1 4.5 99.3 99.7 89.9 90.0 90.0 89.9 91.9 91.9 90.2 91.5 91.9 91.9 96.2 89.6 85.2 87.2 85.9 89.1 99.5 87.2 32 D02-48860(4-8) Crescent Pork
33 0.8 0.5 0.5 10.3 0.8 16.4 5.6 1.0 0.7 0.7 10.1 0.7 10.5 9.9 1.0 1.0 0.8 6.9 10.0 13.9 5.4 9.0 13.9 10.5 10.3 10.7 10.5 10.1 10.1 10.3 4.8 0.7 99.7 89.9 90.0 90.0 89.9 91.9 91.9 90.2 91.5 91.9 91.9 95.9 89.4 84.7 87.2 85.9 89.9 99.5 87.2 33 D02-45742-1 Black Jack Pork
34 0.5 0.2 0.2 10.7 0.5 16.8 5.2 0.7 0.3 0.3 10.5 0.7 10.9 10.3 0.7 0.7 0.5 6.5 10.4 13.5 5.0 9.4 13.5 10.9 10.3 10.7 10.9 10.5 10.5 10.7 4.5 0.3 0.3 89.9 90.0 90.0 89.9 91.9 91.9 90.2 91.5 91.9 91.9 96.2 89.4 85.1 87.2 85.9 89.6 99.8 87.2 34 D02-45742-2 Black Jack Pork
35 10.9 10.5 10.5 12.7 10.5 17.7 13.8 10.8 10.6 10.7 13.0 10.3 13.4 12.8 10.7 10.5 10.5 14.5 12.3 13.6 13.2 11.7 13.6 12.8 4.5 4.8 13.0 12.5 12.5 12.3 13.0 10.3 10.3 10.3 99.5 99.7 95.5 96.8 96.8 95.7 96.7 96.8 96.8 87.9 94.5 86.2 84.4 82.9 87.7 89.7 84.4 35 D04-49972-1 Illini Swine Inc
36 10.9 10.5 10.5 12.3 10.5 17.6 13.8 10.7 10.5 10.7 12.9 10.3 13.3 12.7 10.7 10.5 10.5 14.4 12.3 13.5 13.1 11.6 13.5 12.7 4.5 4.8 12.9 12.5 12.5 12.3 12.9 10.3 10.3 10.3 0.3 99.7 95.7 97.0 97.0 95.9 96.8 97.0 97.0 88.1 94.7 86.4 84.9 83.3 87.9 89.9 84.9 36 D04-49972-2 Illini Swine Inc
37 10.9 10.5 10.5 12.7 10.5 17.6 13.8 10.7 10.5 10.7 12.9 10.3 13.3 12.7 10.7 10.5 10.5 14.5 12.3 13.5 13.2 11.6 13.5 12.7 4.5 4.8 12.9 12.5 12.5 12.3 13.0 10.3 10.3 10.3 0.2 0.3 95.7 97.0 97.0 95.9 96.8 97.0 97.0 88.1 94.7 86.4 84.6 82.9 87.9 89.9 84.6 37 D04-49972-3 Illini Swine Inc
38 10.9 10.5 10.5 12.9 10.1 17.9 13.2 10.4 10.6 9.9 12.8 10.3 13.6 12.5 10.7 10.1 10.1 14.3 12.7 14.8 13.0 11.6 14.8 13.0 0.7 3.6 13.2 12.8 12.8 13.0 12.8 10.3 10.3 10.3 4.5 4.5 4.5 97.8 97.8 99.5 97.7 97.8 97.8 88.1 95.2 86.9 85.2 84.1 87.7 89.7 85.2 38 D03-6295-3 Illini-North
39 9.1 8.8 8.8 11.1 8.4 16.8 12.6 8.6 8.8 8.6 11.3 8.6 11.7 11.1 9.0 8.4 8.8 13.2 10.8 13.1 11.9 9.8 13.1 11.1 2.2 2.7 11.3 10.9 10.9 11.1 11.7 8.6 8.6 8.6 2.9 2.9 2.9 2.2 100.0 98.0 99.8 100.0 100.0 89.7 96.2 88.1 86.2 84.7 89.4 91.7 86.2 39 D03-5293(2-4) I llini Swine-Hich Tec
40 9.1 8.8 8.8 11.1 8.4 16.8 12.6 8.6 8.8 8.6 11.3 8.6 11.7 11.1 9.0 8.4 8.8 13.2 10.8 13.1 11.9 9.8 13.1 11.1 2.2 2.7 11.3 10.9 10.9 11.1 11.7 8.6 8.6 8.6 2.9 2.9 2.9 2.2 0.0 98.0 99.8 100.0 100.0 89.7 96.2 88.1 86.2 84.7 89.4 91.7 86.2 40 D03-5293(5-7) I llini Swine-High Tec
41 10.3 10.0 9.9 12.3 9.5 17.5 12.6 9.8 10.0 9.4 12.2 9.8 13.0 11.9 10.2 9.5 9.6 13.6 12.1 14.4 12.4 11.0 14.4 12.4 0.3 3.2 12.5 12.2 12.2 12.4 12.2 9.8 9.8 9.8 4.1 4.1 4.1 0.3 1.9 1.9 97.8 98.0 98.0 88.4 95.7 87.4 85.6 84.2 88.1 90.0 85.6 41 D03-5296(3-4) I llini Swine-North
42 9.3 9.0 8.9 11.3 8.6 16.6 12.8 8.8 9.0 8.8 11.5 8.8 11.9 11.3 9.2 8.6 9.0 13.4 11.1 13.3 12.1 9.8 13.3 11.3 2.4 2.5 11.5 11.1 11.1 11.3 12.0 8.8 8.8 8.8 3.1 3.1 3.1 2.4 0.2 0.2 2.0 99.8 99.8 89.6 96.0 87.7 85.9 84.4 89.1 91.4 85.9 42 D02-61015-3 Illini Farms South
43 9.1 8.8 8.8 11.1 8.4 16.8 12.6 8.6 8.8 8.6 11.3 8.6 11.7 11.1 9.0 8.4 8.8 13.2 10.8 13.1 11.9 9.8 13.1 11.1 2.2 2.7 11.3 10.9 10.9 11.1 11.7 8.6 8.6 8.6 2.9 2.9 2.9 2.2 0.0 0.0 1.9 0.2 100.0 89.7 96.2 88.1 86.2 84.7 89.4 91.7 86.2 43 D02-61016-1 Illini Farms South
44 9.1 8.8 8.8 11.1 8.4 16.8 12.6 8.6 8.8 8.6 11.3 8.6 11.7 11.1 9.0 8.4 8.8 13.2 10.8 13.1 11.9 9.8 13.1 11.1 2.2 2.7 11.3 10.9 10.9 11.1 11.7 8.6 8.6 8.6 2.9 2.9 2.9 2.2 0.0 0.0 1.9 0.2 0.0 89.7 96.2 88.1 86.2 84.7 89.4 91.7 86.2 44 D02-48857(5-8) I llini South
45 4.1 3.8 3.6 13.5 3.6 16.9 2.2 3.8 4.0 3.8 13.3 4.5 13.5 13.1 4.5 3.8 4.3 3.4 13.0 16.9 1.5 12.2 16.9 13.5 12.5 12.7 13.7 13.3 13.3 13.5 1.0 3.8 4.1 3.8 12.8 12.7 12.8 12.6 11.1 11.1 12.0 11.3 11.1 11.1 87.9 83.6 85.6 84.1 87.2 96.2 85.6 45 D02-48858(7-9) S&S Farms
46 10.9 10.9 10.9 12.2 10.5 18.0 13.4 10.8 11.0 10.7 12.8 10.7 13.4 12.6 11.1 10.5 10.9 14.3 12.1 15.3 13.0 10.9 15.3 12.6 3.8 4.5 12.8 12.4 12.4 12.6 12.8 10.7 10.7 10.7 5.2 5.2 5.2 4.5 3.6 3.6 4.0 3.8 3.6 3.6 12.8 87.9 85.6 83.9 87.9 89.2 85.6 46 D02-47395(1-2) Nipco Farms
47 14.5 14.5 14.5 12.3 14.1 18.5 17.5 13.7 14.6 14.3 13.2 14.3 13.6 13.4 14.3 14.1 14.3 18.0 12.1 13.2 16.9 12.5 13.2 13.2 13.5 14.1 12.9 12.8 12.8 13.0 16.5 14.3 14.7 14.3 14.4 14.4 14.4 13.7 12.7 12.7 13.1 12.9 12.7 12.7 16.9 12.3 84.2 83.1 88.1 84.9 84.2 47 D02-47394(5-8) Prime Pork
48 13.5 13.3 13.3 13.3 13.1 12.8 15.6 13.1 12.9 13.1 12.5 13.1 13.7 12.7 13.1 13.3 12.9 16.5 12.0 16.4 16.2 12.4 16.4 12.9 14.1 14.3 13.3 12.9 12.9 13.1 15.6 13.1 13.1 13.1 15.2 15.2 15.2 14.3 13.7 13.7 13.7 13.9 13.7 13.7 15.6 14.2 16.5 98.7 87.2 87.1 100.0 48 D06-28296-5 Boars
49 13.9 13.7 13.7 13.7 13.5 13.2 16.0 13.5 13.3 13.5 12.8 13.5 14.1 13.1 13.5 13.7 13.3 16.9 12.4 16.6 16.7 12.8 16.6 13.3 14.5 14.7 13.3 13.3 13.3 13.5 16.0 13.5 13.5 13.5 15.7 15.6 15.6 14.5 14.1 14.1 14.1 14.3 14.1 14.1 16.0 14.6 16.7 0.2 85.7 85.7 98.7 49 D06-26717-1 Boars
50 11.0 10.7 10.6 0.5 10.2 15.7 14.7 10.5 10.5 10.5 1.5 10.5 1.9 1.3 10.5 10.2 10.3 15.0 0.8 11.3 13.7 1.4 11.3 1.2 12.5 11.5 1.2 0.8 0.8 1.0 13.3 10.9 10.1 10.5 12.5 12.5 12.5 12.7 10.9 10.9 12.1 11.1 10.9 10.9 13.3 12.4 12.5 13.1 13.5 89.4 87.2 50 IngelvacATP
51 0.3 0.0 0.2 10.9 0.7 17.0 5.2 0.8 0.5 0.5 10.7 0.8 11.1 10.5 0.8 0.8 0.7 6.5 10.6 13.7 5.0 9.6 13.7 11.1 10.5 10.9 11.1 10.7 10.7 10.9 4.5 0.5 0.5 0.2 10.5 10.5 10.5 10.5 8.8 8.8 10.0 9.0 8.8 8.8 3.8 10.9 14.5 13.3 13.7 10.7 87.1 51 RespPRRS
52 13.5 13.3 13.3 13.3 13.1 12.8 15.6 13.1 12.9 13.1 12.5 13.1 13.7 12.7 13.1 13.3 12.9 16.5 12.0 16.4 16.2 12.4 16.4 12.9 14.1 14.3 13.3 12.9 12.9 13.1 15.6 13.1 13.1 13.1 15.2 15.2 15.2 14.3 13.7 13.7 13.7 13.9 13.7 13.7 15.6 14.2 16.5 0.0 0.2 13.1 13.3 52 D06-30617-1 FuturePork
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5.14.06
6.2.06
Sequencing and Dendograms
• = Phylogenetic analysis
• Nucleotide sequencing
Genetic Sequencing
• Homologies do not indicate cross-protection
• Homologies do not predict pathogenicity
• Homologies only indicate relatedness
PRRS…SVN (SN)
• Serum Virus Neutralization
• PRRS SVN not used widely
• Possible direct correlation with protective antibodies in vivo
• Alpha and beta procedures
• Beta utilized for PRRS
SVN…Procedure
• Beta Procedure– Known levels of standard virus incubated with
serial dilutions of test serum– Serum is then added to sensitive cell lines– Titer of serum = Highest dilution of serum that
neutralizes known dose of virus– End points are shown by cytopathology
SVN Results
• Less sensitive than IFA or ELISA– Neutralizing Ab produced slow PI ( >21 days)– Levels of nAb are pig-dependent
• Detectable 9-28 days PI
• Max titers at 2-3 months PI
• Persistent titers > 1 year PI
SVN…Interpretation
• Assumed that shedding of virus is nill if nAb is present
• However, PRRS produces carrier-state animals– REMEMBER:
•ARTERIVIRUS
PRRSv Detection in Tissues
• Histopathology
• PCR
• VI
• IHC
• DFA
PRRS…Histopathology
• Interstitial pneumonia– Thickened alveolar walls– Pneumocyte hypertrophy/hyperplasia– Alveolar spaces filled with necrotic debris and
WBC’s
PRRS…Histopathology
Normal Interstitial pneumonia
T. Opriessnig, 2004
PCR…Process
RNA
Reverse Transcriptase
DNA
Heat Denaturing
Primersand
Cooling
Annealing
Taq +Nucleotides
CT G
A
Complementary Strands
30 Cycles
PRRS…VI
• Virus Isolation
• Lung, Tonsil, Lymph nodes
• Autolysis greatly affects sensitivity
• Much easier to find in serum
PRRS…IHC
• Immunohistochemistry
• Antibody coupled to horseradish peroxidase
• H2O2/Benzidine derivative added
• Colored insoluble precipitate formed
Halbur, AASV
PRRS…DFA
• Direct Fluorescent Antibody
• PRRSv infected tissue
• Fluorescein conjugated to antiviral antibody
DFA…Results
• Quick, Cheap
• Specific
• Less sensitive…autolysis
Semen PRRSv PCR
• Specific
• Less Sensitive – Intermittent Shedding– Detectable Levels > Infectious Levels
Semen PRRSv PCR
Other Respiratory Diagnostics
• I request many of the same diagnostic tests for different respiratory diseases.
• If you feel like you are lost when you are out in practice, call the lab you are working with and the diagnosticians are more than happy to help you out and explain more about what they do.
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