přenosy jader j. fulka, jr. cbttn a vÚŽv praha fertilization – transformation of meiotic cells...

Přenosy jader

J. Fulka, Jr.

CBTTN a VÚŽV

Praha

Fertilization – transformation of meiotic cells into mitotic cells

Gametes – terminally differentiated and highly specialized

Genomes - (oocyte, sperm) transcriptionally inactive after fertilization

• One cell stage embryo is totipotent

• Fertilization represents RADICAL REPROGRAMMING OF BOTH GENOMES

A) Sheep

B) Mouse

C) Human

D) Rabbit

E) Bovine

F) Pig

G) Monkey

Mouse embryo

Human embryo

PB

MPNFPN

FPN

MPN

5-MeC

Human embryo

PB

PN

PN MPN

FPN

49% of human 1-cell stage embryos showed abnormal demethylation pattern

2PB

FP

MP

MP

Wilmut, I et al. (1997)

Viable offspring derived from fetal and adult mammalian cells.

Nature 385, 810-813

A

B

AB

AB

AB

AB

Enukleace

Přenos jádra

What do we need??

Oct4 Cdx2

DAPI merge

Nanog

Oct4 DAPI

DifferentiationβIII tubulin

TROMA 1

DAPI

MF20

+ EB formation

Problém: 300 oocytů na jednu linii/ 20 – 30 dárkyň

Pacient ESc linie

Producing primate embryonic stem cells bysomatic cell nuclear transfer

Nature - doi:10.1038/nature06357

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