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PRACTICAL PHARMACOLOGY-1 (Po701) Third Year
FACULTY OF PHARMACY
Department of Pharmacology & Toxicology
CAIRO UNIVERSITY
شعارالكلية مصر في الصيدلي التعليم منارة
اإلقليمي ومحيطها
Determination of the site of action of
some skeletal muscle stimulants on
Isolated Frog Rectus Abdominis
Why Ach contracts the skeletal muscle?
Why carbachol contracts the skeletal muscle?
Why K + contracts the skeletal muscle?
Aim:Determining the following properties of an unknown drug which causes skeletal muscle contraction:
1. Its susceptibility to destruction by cholinesterase enzyme (CE).
2. Its site of action (acts on Nm receptors or directly on the muscle).
Qualitative experiment
Unknown drug Susceptibility to CE enzyme
Action on Nm receptors
Cholinester (e.g. Ach) + +Carbamate ester(e.g. carbachol) _ +Direct skeletal muscle stimulant (e.g. K+) _ _
Using anti-cholinesterase drug:
Incubate the muscle with PHYSOSTIGMINE (anti-cholinesterase) then add the drug:
if it is hydrolysed by CE if it is not hydrolysed by CE
PHYSOSTIGMINE will prevent its destruction
↑ its amount at receptor site potentiation of muscle response
PHYSOSTIGMINE will not change muscle response
Block Nm receptors by TUBOCURARINE (neuromuscular blocker), then add the drug:
if it acts on Nm receptors
muscle response
will ↓
if it doesn't act on Nm receptors
muscle response will not change
In this experiment:
The muscle’s response to the drug before & after adding the experimental tools (PHYSOSTIGMINR, & TUBOCURARINE) is observed.
Q. Can we add the SM dose as a reference dose only once at the beginning of the experiment?
Ans. No, because the sensitivity of the muscle may change during the experiment leading to changes in muscle response.
0.2 U
0.2 U
0.2 U
0.2 U
0.4 U
0.8 U
0.2 U
0.2 AntiChEs (Physostigmine)
5 min.
OFF + NO WASH after
0.2 NMB (Tubocurarine)
5 min.
OFF + NO WASH after
1. Susceptibility to destruction by cholinesterase: Not susceptible
2. Site of action: Direct
Unknown may be K+
0.2 U
0.4 U
0.8 U
0.2 U
0.2 U
0.2 U
0.2 U
0.2 AntiChEs (Physostigmine)
5 min.
0.2 NMB (Tubocurarine)
5 min.
OFF + NO WASH after
OFF + NO WASH after
1. Susceptibility to destruction by cholinesterase: Not susceptible
2. Site of action: Stimulates muscular Nm receptors
Unknown may be Carbachol
0.2 U
0.4 U
0.8 U
0.2 U
0.2 U
0.2 U
0.2 U
0.2 AntiChEs (Physostigmine)
5 min.
0.2 NMB (Tubocurarine)
5 min.
OFF + NO WASH after
OFF + NO WASH after
1. Susceptibility to destruction by cholinesterase: Susceptible
2. Site of action: Stimulates muscular Nm receptors
Unknown may be ACh
Isolated Frog Rectus Abdominis
Experiment:Determination of the site of action of some skeletal muscle stimulants
STEPS
STEPS 8Effect of anticholinesterase “physostigmine”:1. Choose a submax. dose2. Repeat addition of submax. dose after the D/R3. Incubate rectus with “physostigmine” 4. Repeat addition of submax. on incubated rectus
Physostigmine
Tubocurarine
Physostigmine
STEPS 9Effect of neuromuscular blocker “tubocurarine”
Repeat the previous steps but with “tubocurarine”
ΔΜϟΎΜϟ :Δϴγ έΪ ϟΔϗήϔϟ
ϡΎόϟϲ όϣΎΠϟ: 2014/ 2015 :ϰ γ έΪ ϟϞμ ϔϟβ ϣΎΨϟ
(PO 701) -ΔϳϭΩϷϢϠϋέήϘϣ1
Ϣδ ϗϷϡϮϤδ ϟϭΔϳϭΩ
ΩΎόϴϤϟ Ώϼτ ϟϡΎϗέ ϢϟϥΎϛ ϡϮϴϟ
8:30-8:40 odd
Group B
ϡϮϤ
δϟϭΔ
ϳϭΩϷ
ϞϣΎόϣ
(αΩΎδ
ϟέϭΪϟ)
ΖΒδ
ϟ
20/12/2014
8:50-9:00 even
11:30-11:40 odd
Group A
11:50-12:00 even
ϰ ϠϋΏϼτ ϟ ϊ ϳίϮΗϲ ϠϤόϟϱ ήψϧϥΎΤΘϣ
έήϘϤϟϖ δ Ϩϣ
Ω.ΐ ϴτ ΨϟϦϤϳ /
Pharmacology – I (PO 701)
The prelabs will be held in the pharmacology labs (sixth floor) before the ICDL labs
Time 9:45-10:15 (odd)
10:30-11
(even)
12:15 -12:45 (odd)
1-1:30 (even) Day Lab
Satu
rday
29/11-
6/1
2/2
014
ICDL Ground floor
˻ ˹ ˺ ˻ ˺ ̀ ˺ -˻ ˹ ˺ ˻ ˻ ˾ ˾
˻ ˹ ˺ ˻ ˺ ̀ ˺ -˻ ˹ ˺ ˻ ˻ ˾ ˾
˻ ˹ ˺ ˺ ˺ ˹ ́ -˻ ˹ ˺ ˺ ˺ ˽ ˾ -˻ ˹ ˺ ˺ ˺ ˽ ́ -2011248-˻ ˹ ˹ ́ ˺ ˻ ̂ -˻ ˹ ˺ ˺ ˹ ˹ ́ -
2012001-2012081
˻ ˹ ˺ ˺ ˺ ˹ ́-˻ ˹ ˺ ˺ ˺ ˽ ˾-˻ ˹ ˺ ˺ ˺ ˽ ́-2011248-˻ ˹ ˹ ́ ˺ ˻ ̂-˻ ˹ ˺ ˺ ˹ ˹ ́-
2012001-2012081
New ICDL lab Beside Clinical Program
Unit
2012256-2012341
2012256-2012341
2012082-2012170 2012082-2012170
FACULTY OF PHARMACY DEPARTMENT OF PHARMACOLOGY &
TOXICOLOGY
CAIRO UNIVERSITY Academic Year: 2014/2015
Semester: Five Clinical Pharmacy Program
Student’s Distribution in ICDL Labs
Pharmacology – I (PO 701)
Student Numbers
2012171-2012255
(odd)
2012171-2012255
(even) 2012256-2012341
(odd)
2012256-2012341 (even) Day Hour
Satu
rday
13/1
2/2
014
1st Hour (9 am-10 am)
Pharmacology
Labs ( 6th floor)
ICDL (Ground floor)
Pharmacology Labs ( 6th floor)
New ICDL lab Beside Clinical
Program Unit
2nd Hour (10 am -11 am)
ICDL (Ground floor)
Pharmacology Labs ( 6th
floor)
New ICDL lab Beside Clinical
Program Unit
Pharmacology Labs ( 6th floor)
FACULTY OF PHARMACY DEPARTMENT OF PHARMACOLOGY &
TOXICOLOGY
CAIRO UNIVERSITY Academic Year: 2014/2015
Semester: Five Clinical Pharmacy Program
Student’s Distribution in Labs 13/ 12/ 2014
COURSE COORDINATOR
PROF. DR. AIMAN S. EL-KHATIB
Pharmacology – I (PO 701)
Student Numbers 2011108-2011145-2011148-2011248-2008129-2011008-2012001-2012081
(odd)
2011108-2011145-2011148-2011248-2008129-2011008-2012001-2012081
(even)
2012082-2012170
(odd)
2012082-2012170 (even)
Day Hour
Sat
urd
ay
13/1
2/2
014
1st Hour
(11:30am-12:30 pm)
Pharmacology Labs ( 6th floor)
ICDL (Ground floor) Pharmacology
Labs ( 6th floor)
New ICDL lab Beside Clinical
Program Unit
2nd Hour (12:30 pm -1:30 pm)
ICDL (Ground floor)
Pharmacology Labs ( 6th floor)
New ICDL lab Beside Clinical
Program Unit
Pharmacology Labs ( 6th floor)
FACULTY OF PHARMACY DEPARTMENT OF PHARMACOLOGY &
TOXICOLOGY
CAIRO UNIVERSITY Academic Year: 2014/2015
Semester: Five Clinical Pharmacy Program
COURSE COORDINATOR
PROF. DR. AIMAN S. EL-KHATIB
Student’s Distribution in Labs 13/ 12/ 2014
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