photosynthesis watch?v=tshmwiz9fnw

PHOTOSYNTHESIS

http://www.youtube.com/watch?v=tSHmwIZ9FNw

Objectives Explain plant physiology concepts and

energy conversion in plants. a) Explain the basic process of photosynthesis

and its importance to life on Earth. b) Explain requirements necessary for

photosynthesis to occur, and identify the products and byproducts of photosynthesis.

c)Explain cellular respiration and its importance to plant life.

d)Explain factors that affect cellular respiration, and identify the products and byproducts of cellular respiration.

• Almost all plants are photosynthetic autotrophs, as are some bacteria and protists

– Autotrophs generate their own organic matter through photosynthesis

– Sunlight energy is transformed to energy stored in the form of chemical bonds

(a) Mosses, ferns, andflowering plants

(b) Kelp

(c) Euglena (d) Cyanobacteria

THE BASICS OF PHOTOSYNTHESIS

Light Energy Harvested by Plants & Other Photosynthetic Autotrophs

6 CO2 + 6 H2O + light energy → C6H12O6 + 6 O2

WHYWHY ARE ARE PLA PLANTS NTS GREGREEN?EN?

Plant Cells have Green Chloroplasts

The thylakoid membrane of the chloroplast is impregnated with photosynthetic pigments (i.e., chlorophylls, carotenoids).

Chloroplasts absorb light energy and convert it to chemical energy

LightReflected

light

Absorbedlight

Transmittedlight

Chloroplast

THE COLOR OF LIGHT SEEN IS THE COLOR NOT ABSORBED

Photosynthesis is the process by which autotrophic organisms use light energy to make sugar and oxygen gas from carbon dioxide and water

AN OVERVIEW OF PHOTOSYNTHESIS

Carbondioxide

Water Glucose Oxygengas

PHOTOSYNTHESIS

• The Calvin cycle makes sugar from carbon dioxide

– ATP generated by the light reactions provides the energy for sugar synthesis

– The NADPH produced by the light reactions provides the electrons for the reduction of carbon dioxide to glucose

LightChloroplast

Lightreactions

Calvincycle

NADP

ADP+ P

The light reactions convert solar energy to chemical energy Produce ATP & NADPH

AN OVERVIEW OF PHOTOSYNTHESIS

Steps of Photosynthesis Light hits reaction centers of chlorophyll, found

in chloroplasts

• Chlorophyll vibrates and causes water to break apart.

• Oxygen is released into air• Hydrogen remains in chloroplast

attached to NADPH• “THE LIGHT REACTION”

Steps of Photosynthesis The DARK Reactions= Calvin Cycle

• CO2 from atmosphere is joined to H from water molecules (NADPH) to form glucose

• Glucose can be converted into other molecules with yummy flavors!

Photosynthesis occurs in chloroplasts

In most plants, photosynthesis occurs primarily in the leaves, in the chloroplasts

A chloroplast contains: stroma, a fluid grana, stacks of thylakoids

The thylakoids contain chlorophyll Chlorophyll is the green pigment that captures light for

photosynthesis

The location and structure of chloroplasts

LEAF CROSS SECTION MESOPHYLL CELL

LEAF

Chloroplast

Mesophyll

CHLOROPLAST Intermembrane space

Outermembrane

Innermembrane

ThylakoidcompartmentThylakoidStroma

Granum

StromaGrana

Chloroplast Pigments Chloroplasts contain several pigments

– Chlorophyll a – Chlorophyll b – Carotenoids– Xanthophyll

Figure 7.7

Chlorophyll a & b•Chl a has a methyl group •Chl b has a carbonyl group

Porphyrin ring delocalized e-

Phytol tail

Different pigments absorb light differently

Cyclic Photophosphorylation

Process for ATP generation associated with some Photosynthetic Bacteria

Ph

oto

n

Ph

oto

n

Water-splittingphotosystem

NADPH-producingphotosystem

ATPmill

Two types of photosystems cooperate in the light reactions

Primaryelectron acceptor

Primaryelectron acceptor

Electron transport chain

Electron transport

Photons

PHOTOSYSTEM I

PHOTOSYSTEM II

Energy forsynthesis of

by chemiosmosis

Noncyclic Photophosphorylation

Photosystem II regains electrons by splitting water, leaving O2 gas as a by-product

Plants produce OPlants produce O22 gas by splitting H gas by splitting H22OO

The O2 liberated by photosynthesis is made from the oxygen in water (H+ and e-)

In the light reactions, electron transport In the light reactions, electron transport chains generate ATP, NADPH, & Ochains generate ATP, NADPH, & O22

Two connected photosystems collect photons of light and transfer the energy to chlorophyll electrons

The excited electrons are passed from the primary electron acceptor to electron transport chains Their energy ends up in ATP and NADPH

Chemiosmosis powers ATP synthesis in the light reactions

The electron transport chains are arranged with the photosystems in the thylakoid membranes and pump H+ through that membrane The flow of H+ back through the membrane is

harnessed by ATP synthase to make ATP In the stroma, the H+ ions combine with NADP+ to form

NADPH

2 H + 1/2

Water-splittingphotosystem

Reaction-center

chlorophyll

Light

Primaryelectronacceptor

Energyto make

Electron transport chain

Primaryelectronacceptor

Primaryelectronacceptor

NADPH-producingphotosystem

Light

NADP

1

23

How the Light Reactions Generate ATP and NADPH

Video to help http://www.youtube.com/watch?

v=hj_WKgnL6MI

Summary—Light Dependent Reactions

a. Overall inputlight energy, H2O.

b. Overall output ATP, NADPH, O2.

Animation is of the Calvin Cycle Note what happens to the carbon dioxide and what the end product is.

Second animation of the Calvin Cycle is very clear and even does the molecular bookkeeping for you.

Light Independent Reactions aka Calvin Cycle

Carbon from CO2 is converted to glucose

(ATP and NADPH drive the reduction

of CO2 to C6H12O6.)

Light Independent Reactions aka Calvin CycleCO2 is added to the 5-C sugar RuBP by the

enzyme rubisco.This unstable 6-C compound splits to two

molecules of PGA or 3-phosphoglyceric acid.

PGA is converted to Glyceraldehyde 3-phosphate (G3P), two of which bond to form glucose.

G3P is the 3-C sugar formed by three turns of the cycle.

Video to help http://www.youtube.com/watch?

v=o1I33Dgcc_M

Summary—Light Independent Reactions a. Overall input CO2, ATP, NADPH.

b. Overall output glucose.

Why high heat? When it is hot, plants close their stomata to

conserve water They continue to do photosynthesis use up

CO2 and produce O2 creates high O2 concentrations inside the plant photorespiration occurs

Review: Photosynthesis uses light energy to make food molecules

A summary of the chemical processes of photosynthesis

Light

Chloroplast

Photosystem IIElectron transport

chains Photosystem I

CALVIN CYCLE Stroma

Electrons

LIGHT REACTIONS CALVIN CYCLE

Cellular respiration

Cellulose

Starch

Other organic compounds