nanotechnology and health. the use of nanotechnology ... · of nanotechnology, photoprocess and...

Nanotechnology and Health. The use

of Nanotechnology, Photoprocess

and Tissue Engineering to treat skin

cancer and other diseases

Prof. Dr. Antonio Claudio Tedesco, Center of Nanotechnology and Tissue Engineers

Photobiology and Photomedicine research Group

FFCLRP- São Paulo UniversityPreto, SP

atedesco@usp.br

www.nanotecnologiafotomedicina.org

www.centronanotecengtecidual.org

Nanotechnology

and Heath

Nanomedicine

The reality

• Dentistry

Skin Cancer

Tissue regeneration

• Cancer

• Tissue Engineer

2003

2007

2000 RP-SP

2006

Brasília -2006

UNIFESP -2007

INPA -2009

Nanotecnology

Photoprocess in Cancer

treatment (PDT)

Visibel Ligth and PDT

Tissue Regeneration and PDT

Silva et al Laser in Surgery and Medicine, 34 (2005), 451.

Bautista-Sanchez et al Journal of Photochemistry and Photobiology B: Biology

81(2006), 154

Figure

8.8 N

NH N

HN

OpMe

OR

N

NN

N

N

NN N

MeO

O

Zn

N

NH N

HN

R1

R2R3

R4

N N

N

N

N

N

N

N

Zn

N

OHOH

O

O N

N

N

OH

O

HO

OHO

O

OH

HO

O

O

HO

O

Synthetic Photoactive

compounds

Liposomes

Nanoparticules

Nanoemulsion

Dendrimers

Nanospheres

Magnetic

Nanocomposite

Proteins Nanoparticle

Drug Delivery

Control release

www.metodoeventosrio.com/riopharma/apresentacoes/Branco/Sexta/.../RioPharma%202

007.ppt

FS FS

Liposome as a Drug Delivery System

LDL e LDL-lipidic -polimeric Drug Delivery

Lisossomo

Secundário Amino

ácidos

Colesterol

Ácido

Graxo

Partícula

de LDL

com Chol

Membrana

Plásmatica

Receptores de

LDL

Polimeric Nanoparticles and

Carbon Nanotubes

pt-pt.facebook.com

Neurodegenerative diseases

Parkinson

Alzheimer

Epilepsy

•neurological degenerative diseases,

•neuro-orthopedical problems

Published online 25 May 2011 | Nature |

doi:10.1038/news.2011.319

Engineered antibodies

cross blood–brain barrier

Glioma e Glioblastoma the

signalization and treatment

15

Photo sensitizers molecules associated

with magnetic nanoparticles as synergic

DDS

Magnetic Nanocarriers

Selol

- Lower toxicity

- Higher Se doses

- Lipophilicity cell

uptake

Tissue Engineering

Dermal Equivalent Preparation

Schematic illustration of the DE formation steps. a) fibroblast

monolayer culture in DMEM; b) collagen type I addition, c)

fibroblast ramification; d) fibroblast+colagen on 3D network; e)

skin layers formation and f) Human Dermal Equivalent.

Schematic representation of AlClPC-nanoemulsion preparation.

Nanomaterial Preparation

50 nm

Morphological Analysis of Nanomaterial: TEM (Transmission Electron Microscopy)

Morphological analysis by TEM images for ClAlPc/NE loaded at 0.025 mg/mL.

High-resolution at 70 Kv and increased of 36.000x (a) and 71.000x (b).

A) B)

Steam Cells and skin 3D model

Human Skin Dermal Equivalent

(A) Human gingival fibroblasts: in vitro monolayer culture and

(B) Skin humam Dermal Equivalent (DE)

A) B)

Collagen matrix micrographs of DE and DE-BMMSC culture. Fibroblast monolayer

culture (A); 3D-network collagen in 3 days (B); 3D network collagen in 7 days and

inset DE disc (C); dense 3D-network collagen covered with BMMSC (D); GFP-

BMMSC fluorescence microscopy with excitation at 395 nm and emission at 509 nm

(E) and GFP-BMMSC and 3D collagen overlapping.

Wound Healing Process

“Rebuilding” Blood Vessel

Blood Vessel Reconstructions

Future in the application of

Nanotechnology and Health

Design and synthesis of new drugs,

potentially active , in wound healing and

skin diseases,

Move to the New Frontiers'... of Science as

Nanotechnology , DDS, Tissue Eng.

Photoprocess

Use of steam cells in conjunction of 3D skin

model to reconstruction of blood vessel

and other tissue.

Clinical trial in other non-neoplastic

application.

In vitro, in vivo studies

Nanostructure production

50 -100 cm2 3D skin

production

Photo process spectroscopy

analysis

500 m2 from bench to scaling

up

•Photobiology and Photomedicine Research

•FFCL Ribeirão Preto-USP

•Grupo de Pesquisa em Fotobilogia e Fotomedicina .

•FFCLRibeirão Preto- USP

•tedesco@ffclrp.usp.br or atedesco @usp.br

•PhD and Pos-Doc

•Fernando Lucas Primo

•Fernanda Zampieri Leandro

•Adrielle Castilho Fernandes

•Marigilson Siqueira Moura

•Maristela F Sanches Peres

•Doutorandos

•Natalia F Monteiro

•Gisele C. Bolfarini

•Daniela Maranhos

•Leonardo Barcelos Paula

•Aldo Aparicio Acosta

•Mestrandos

•Caroline Vacari

•Hugo Menezes

•Andre Miotello

•Priscila C C de Jesus

•Camila Vizentini Silva

•IC

•Yves Yoshinori Saga

. Camila F Amantino

•Sabrina Ikeda Neves

•João Marcos Gonçalves

•Júlia Martins

•Daniela Regina Jardim

•Ana carolina S Marofioti

•FFCLRP-USP

•Marilda Assis das Dores

FMRP-USP

Enilza M Espreafico

•UNIVAP

•Milton Beltrame Junior

•Andreza Ribeiro Simioni

•Cristina Pacheco

•HC-USP – IFUSP SC

•Dra Cacilda C. Souza

•Vanderlei S. Bagnato

•UnGo

•Emilia C.D. Lima

•UNB

•Paulo C. Morais

•Ricardo Bentes Azevedo

•Maria Sueli Felipe

•Zulmira G. M. Lacava

•Anamelia L Bocca

•Dra Simone Karst

•UFOP UFMG

• .Dr a Vanessa C F Mosqueira

•.Dr a Monica C de Oliveira

•Inst of Phys, Polish Acad of Sciences &

University, Varsóvia, Polônia

•P. Suchocki

•Ewa. Mosiniewicz-Szablewska

•Université Pierre et Marie Curie & Paris

• .Dr Bernard Coulomb

•Institut Curie (Orsay) França

• Dr. Philippe Maillard

•Uruguai Dep. Neonatología Fac. de

Medicina, Montevideo

•Profa. Dra. Maria F. Blasina

•Prof. Dr. Federico Dajas

•UNIFESP

•Fernando A de

Almeida

•.Mauro Y Enokihara

•.Jame Tomimori

INCT

Nanotecnologia