nanotechnology and health. the use of nanotechnology ... · of nanotechnology, photoprocess and...
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Nanotechnology and Health. The use
of Nanotechnology, Photoprocess
and Tissue Engineering to treat skin
cancer and other diseases
Prof. Dr. Antonio Claudio Tedesco, Center of Nanotechnology and Tissue Engineers
Photobiology and Photomedicine research Group
FFCLRP- São Paulo UniversityPreto, SP
www.nanotecnologiafotomedicina.org
www.centronanotecengtecidual.org
Nanotechnology
and Heath
Nanomedicine
The reality
• Dentistry
Skin Cancer
Tissue regeneration
• Cancer
• Tissue Engineer
2003
2007
2000 RP-SP
2006
Brasília -2006
UNIFESP -2007
INPA -2009
Nanotecnology
Photoprocess in Cancer
treatment (PDT)
Visibel Ligth and PDT
Tissue Regeneration and PDT
Silva et al Laser in Surgery and Medicine, 34 (2005), 451.
Bautista-Sanchez et al Journal of Photochemistry and Photobiology B: Biology
81(2006), 154
Figure
8.8 N
NH N
HN
OpMe
OR
N
NN
N
N
NN N
MeO
O
Zn
N
NH N
HN
R1
R2R3
R4
N N
N
N
N
N
N
N
Zn
N
OHOH
O
O N
N
N
OH
O
HO
OHO
O
OH
HO
O
O
HO
O
Synthetic Photoactive
compounds
Liposomes
Nanoparticules
Nanoemulsion
Dendrimers
Nanospheres
Magnetic
Nanocomposite
Proteins Nanoparticle
Drug Delivery
Control release
www.metodoeventosrio.com/riopharma/apresentacoes/Branco/Sexta/.../RioPharma%202
007.ppt
FS FS
Liposome as a Drug Delivery System
LDL e LDL-lipidic -polimeric Drug Delivery
Lisossomo
Secundário Amino
ácidos
Colesterol
Ácido
Graxo
Partícula
de LDL
com Chol
Membrana
Plásmatica
Receptores de
LDL
Polimeric Nanoparticles and
Carbon Nanotubes
pt-pt.facebook.com
Neurodegenerative diseases
Parkinson
Alzheimer
Epilepsy
•neurological degenerative diseases,
•neuro-orthopedical problems
Published online 25 May 2011 | Nature |
doi:10.1038/news.2011.319
Engineered antibodies
cross blood–brain barrier
Glioma e Glioblastoma the
signalization and treatment
15
Photo sensitizers molecules associated
with magnetic nanoparticles as synergic
DDS
Magnetic Nanocarriers
Selol
- Lower toxicity
- Higher Se doses
- Lipophilicity cell
uptake
Tissue Engineering
Dermal Equivalent Preparation
Schematic illustration of the DE formation steps. a) fibroblast
monolayer culture in DMEM; b) collagen type I addition, c)
fibroblast ramification; d) fibroblast+colagen on 3D network; e)
skin layers formation and f) Human Dermal Equivalent.
Schematic representation of AlClPC-nanoemulsion preparation.
Nanomaterial Preparation
50 nm
Morphological Analysis of Nanomaterial: TEM (Transmission Electron Microscopy)
Morphological analysis by TEM images for ClAlPc/NE loaded at 0.025 mg/mL.
High-resolution at 70 Kv and increased of 36.000x (a) and 71.000x (b).
A) B)
Steam Cells and skin 3D model
Human Skin Dermal Equivalent
(A) Human gingival fibroblasts: in vitro monolayer culture and
(B) Skin humam Dermal Equivalent (DE)
A) B)
Collagen matrix micrographs of DE and DE-BMMSC culture. Fibroblast monolayer
culture (A); 3D-network collagen in 3 days (B); 3D network collagen in 7 days and
inset DE disc (C); dense 3D-network collagen covered with BMMSC (D); GFP-
BMMSC fluorescence microscopy with excitation at 395 nm and emission at 509 nm
(E) and GFP-BMMSC and 3D collagen overlapping.
Wound Healing Process
“Rebuilding” Blood Vessel
Blood Vessel Reconstructions
Future in the application of
Nanotechnology and Health
Design and synthesis of new drugs,
potentially active , in wound healing and
skin diseases,
Move to the New Frontiers'... of Science as
Nanotechnology , DDS, Tissue Eng.
Photoprocess
Use of steam cells in conjunction of 3D skin
model to reconstruction of blood vessel
and other tissue.
Clinical trial in other non-neoplastic
application.
In vitro, in vivo studies
Nanostructure production
50 -100 cm2 3D skin
production
Photo process spectroscopy
analysis
500 m2 from bench to scaling
up
•Photobiology and Photomedicine Research
•FFCL Ribeirão Preto-USP
•Grupo de Pesquisa em Fotobilogia e Fotomedicina .
•FFCLRibeirão Preto- USP
•[email protected] or atedesco @usp.br
•PhD and Pos-Doc
•Fernando Lucas Primo
•Fernanda Zampieri Leandro
•Adrielle Castilho Fernandes
•Marigilson Siqueira Moura
•Maristela F Sanches Peres
•Doutorandos
•Natalia F Monteiro
•Gisele C. Bolfarini
•Daniela Maranhos
•Leonardo Barcelos Paula
•Aldo Aparicio Acosta
•Mestrandos
•Caroline Vacari
•Hugo Menezes
•Andre Miotello
•Priscila C C de Jesus
•Camila Vizentini Silva
•IC
•Yves Yoshinori Saga
. Camila F Amantino
•Sabrina Ikeda Neves
•João Marcos Gonçalves
•Júlia Martins
•Daniela Regina Jardim
•Ana carolina S Marofioti
•FFCLRP-USP
•Marilda Assis das Dores
FMRP-USP
Enilza M Espreafico
•UNIVAP
•Milton Beltrame Junior
•Andreza Ribeiro Simioni
•Cristina Pacheco
•HC-USP – IFUSP SC
•Dra Cacilda C. Souza
•Vanderlei S. Bagnato
•UnGo
•Emilia C.D. Lima
•UNB
•Paulo C. Morais
•Ricardo Bentes Azevedo
•Maria Sueli Felipe
•Zulmira G. M. Lacava
•Anamelia L Bocca
•Dra Simone Karst
•UFOP UFMG
• .Dr a Vanessa C F Mosqueira
•.Dr a Monica C de Oliveira
•Inst of Phys, Polish Acad of Sciences &
University, Varsóvia, Polônia
•P. Suchocki
•Ewa. Mosiniewicz-Szablewska
•Université Pierre et Marie Curie & Paris
• .Dr Bernard Coulomb
•Institut Curie (Orsay) França
• Dr. Philippe Maillard
•Uruguai Dep. Neonatología Fac. de
Medicina, Montevideo
•Profa. Dra. Maria F. Blasina
•Prof. Dr. Federico Dajas
•UNIFESP
•Fernando A de
Almeida
•.Mauro Y Enokihara
•.Jame Tomimori
INCT
Nanotecnologia