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Post on 08-Dec-2015
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Material And Methods
Patient identification and recruitmentPatient
identification and recruitment
Sample of Patient receiving statin
Age ≥ 18 years
Increase CPK > 4*ULN
Extraction of DNA
Blood sample 5ml
Saliva sample 3ml
Designing and synthesis of oligonucleotide primers
Primer 3 software
IDT , BLAST(USA)
PCR and Nucleotide
sequencing
Qiagen PCR
Biosystem 3130 XL genetic analyzer
Chromaslite program
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