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A DSIR approved R&D Facility
MOLECULAR BIOLOGY
�gccbiotech.co.in�
GCC�Biotech�(India)�Pvt.�Ltd.
tech.support@gccbiotech.co.ininfo@gccbiotech.co.inoligo@gccbiotech.co.in
�91-33-24951044�/�24950004
Joychandipur,Bakhrahat,�PIN.743377,South�24�Parganas,�W.B.,�INDIA
Super DH 5α Competent Cell
Super XL-1 Blue Competent Cells
Efficomp supercompetent E.coli cells
BL21(DE3) Competent E. coli
BL21(DE3)pLysS Competent Cells
X-Gal solution, 20mg/ml, Ready to use
100mM IPTG solution, Ready to use
X-Gal
IPTG, Dioxane-Free
MOLECULAR Biology
DNA template
(cDNA/gDNA)
Primers/oligos
G9 Taq DNA polymerase
Hi- G9 Taq DNA polymerase
2x G9 Taq PCR Master Mix
2x Hi G9 Taq PCR Master Mix
PrimeTaq DNA Polymerase
Hi-PrimeTaq DNA Polymerase
G9 PCR CORE KIT (G9 Taq)
2x PrimeTaq PCR Master Mix
2x Hi-PrimeTaq PCR Master Mix
Hot Start G9 Taq DNA Polymerase
2X Hot Start G9 Taq DNA Polymerase
Hot Start PrimeTaq DNA Polymerase
2X Hot Start PrimeTaq DNA Polymerase
GPfu DNA Polymerase
2x GPfu Master Mix
Pro Pfu DNA Polymerase (Pfu Turbo)
2x Pro Pfu (Pfu Turbo)Master Mix
DeltaQ Polymerase
dNTP's (100mM dNTP set)
10mM dNTP mix
PCR grade extra pure DMSO
Real time PCR
analysis
2x qPCR mastermix, SYBR, Rox
2X H-eff qPCR master mix, Rox
WRTaqman 2X mastermix
FasTaqman mastermix for Blood
Taqman probes
PCR amplificationAnalysis on
agarose gel
DNA purification
DNA modification
and LigationDNA loading dye
10X TBE Buffer -HI-grade
10X TAE Buffer -HI-grade
High Gel Agarose, Molecular biology grade
Ethidium Bromide solution (10mg/ml)
Ethidium Bromide-Blue solution (10mg/ml)
Super Stain Nucleic Acid Gel Stain -
Ultra (100000x)
DNA Ladder
Bacterial
transformation
T4 Polynucleotide Kinase, Hi-Q
Klenow Fragment
Shrimp Alkaline Phosphatase (SAP)
Alkaline Phosphatase Calf Intestinal (Hi-Q)
T4 DNA Ligase
Hi efficiency TA-Cloning kit
Screening of
clones
Isolation and
purification of DNA
Biochemical
characterisationSite-directed
mutagenesis
Sequence
identification
Protein purificationBacterial transformation/
protein overexpressionGLyseB (Bacterial lysis buffer)
2X Lysis/ equlibration buffer
His Protein Purification-Wash Buffer
Lysozyme (10 mg/ml) in 10 mM
Tris-HCl, pH 8.0
Hi- bind Ni-NTA Agarose Bead
Hi- bind Ni-IDA Agarose Bead
Site Directed Mutagenesis Kit S (6Kb)
Site Directed Mutagenesis Kit L (10Kb)
Pro Pfu DNA Polymerase (Pfu Turbo)
DpnI
Efficomp supercompetent E.coli cells
Gsure® plasmid Mini kit
GSure® Splash Plasmid mini kit
GSure® Rapid plasmid Mini kit
1X Colony PCR Master Mix
1X Colony PCR Mix (T7)
1X Colony PCR Mix (M13)
GSure®Splash Plasmid mini kit
Gsure® PCR Purification Kit
GSure® Gel extraction kit
GSure® Ultra kit is highly efficient to deliver genomic DNA and
total RNA from same sample. The Kit works robustly on wide
range of samples like Plant tissue, animal tissue, cultured cells,
blood and bacteria. This kit provides simple, rapid and
convenient technique to isolate DNA and RNA of high yield from
very small sample volume. The kit is equally efficient on fresh as
well as stored sample in RNA Later.
Different kit is designed for efficient lysis, removal of
contaminants and purification of superior grade nucleic acids
from bacteria, plant, blood, cultured cells and tissue samples.
Buffers of different isolation kits are uniquely formulated for
rapid and efficient lysis of that particular cell type.
GSure® Ultra Nucleic Acid isolation kit delivers total nucleic
acid population of a particular sample, thus makes the
transcriptome analysis more perfect in reference to the genome
content.
GSure® ULTRA kit combines the advantages of a silica-based
microspin column for efficient binding and purity of the
extracted nucleic acid. The kit is a dual column based isolation kit
for selective binding of DNA and RNA with the respective
column.
Purified DNA and RNA are compatible for all sorts of down-
stream application. Purified genomic DNA is suitable for PCR
amplification, Southern blotting, restriction digestions, next
generation sequencing and purified RNA is compatible for RT-
PCR amplification, Northern blotting, RNase Protection assay,
microarray etc.
Description
GSure® Ultra Kit
MOLeCULAR
BIOLOGY1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
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GCC Biotech (India) Pvt. Ltd. | tech.support@gccbiotech.co.in | 91-33-24951044/24950004 | gccbiotech.co.in
GSure® Ultra Nucleic Acid Isolation Kit (Bacteria)
Description
GSure® Ultra Nucleic acid isolation kit (Bacteria) is highly
efficient and specific for isolation of total RNA and DNA from
same bacterial sample. Purified DNA and RNA are free from
contamination and are suitable for down-stream application.
This kit provides a simple and convenient technique to isolate
DNA and RNA of high yield with very small sample volume.
GSure® kit combines the advantages of a silica-based system
with a microspin format, thus eliminates the requirement of
expensive resins and hazardous organic compounds.
Buffers provided with the GSure® Ultra Nucleic acid isolation
kit for bacteria assure complete lysis of bacterial cell wall with
digestion of cellular proteins. Buffer composition is optimized in
such a way that DNA binds selectively to GMini DNA Binding
Spin column and RNA binds selectively to the GMini CHROME
column. The lysis buffer contains Proteinase K thus its external
addition is not required. All the buffers are room temperature
comfortable.
Advantages
ü Highly Purified DNA and RNA from same Bacterial culture
just in 45 min. For purification of genomic DNA and total
RNA from 500 µl bacterial cells.
ü An easy to use silica membrane, spin-mini-column based
DNA-RNA isolation kit for rapid, robust and reproducible
isolation of genomic DNA and total RNA from same
samples.
ü Required sample volume is less and has a easy workflow.
ü Delivers high quality integrated RNA in every isolation.
ü Doesn't retain impurities from sample in the eluted DNA
and RNA.
ü Wide range of starting cell volume.
Ordering Information
Featuresæ Easy – spin column format.
æ Convenient-Same optimized protocol for different sources
of sample.
æ High yield- Recovers 2 µg total gDNA and 5 µg total RNA
from 500 µl bacterial cells.
æ Reproducible-Delivers almost equal amount of yield on
every isolation.
æ Easy to use- No requirement of addition of Proteinase K
externally.
æ Cost effective – More preps for the money.
æ Eco-Friendly- Minimum number of steps, thus minimum
number of plasticware required.
Fig. 1:
DNA and RNA were isolated from different bacterial species. 500 ml of 0.6
O.D cells were harvested and total nucleic acid was isolated. 1/10th
volume of isolated DNA and RNA samples were run on 1% agarose TAE
gel.
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DNA RNA
Cat. # Product Pack Size
GRD1001BGSure® Ultra Nucleic Acid
Isolation kit (Bacteria)20 prep
GRD1001GSure® Ultra Nucleic Acid
Isolation kit (Bacteria)50 prep
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
GCC Biotech (India) Pvt. Ltd. | tech.support@gccbiotech.co.in | 91-33-24951044/24950004 | gccbiotech.co.in 56
GSure® Ultra Nucleic Acid Isolation Kit (Blood)
Description
GSure® Ultra Nucleic acid isolation kit (Blood) is highly efficient
to lyse WBCs and extract extremely purified genomic DNA and
total RNA from Blood sample compatible for all sorts of
downstream applications like PCR amplification, southern
blotting, restriction digestions, next generation sequencing for
isolated DNA and RT-PCR amplification, northern blotting, one
step qRT PCR analysis etc for RNA. Only 200 µl of blood from a
helthy individual is sufficient to isolate 2 µg total gDNA and 0.7
µg total RNA. The kit is so robust that it can isolate DNA and RNA
even from 48 hours properly stored blood. Minimum sample
volume required for isolation is 200 µl and isolation steps are
simple and easy.
The kit comes with RBC Lysis Buffer (RBCL Buffer) to lyse RBC
selectively. The kit can also isolate DNA and RNA from small
volume of total blood samples as well as from buffy coat too.
Advantages
ü Highly Purified DNA and RNA from Blood in 45 min.
ü For purification of genomic DNA and total RNA from
200µl to 1ml Fresh Blood.
ü An easy to use silica membrane, spin-mini-column based
DNA-RNA isolation kit for rapid, robust and reproducible
isolation of genomic DNA and total RNA from same
samples.
ü Required sample volume is less and easy workflow.
ü Equally efficient on fresh as well as stored Blood (up to 48
hours at 4˚C).
ü Delivers high quality integrated RNA in every isolation.
ü Doesn't retain impurities from sample in the eluted DNA
and RNA ?
ü Wide range of starting volume.
Ordering Information
Features
æ Easy – spin column format
æ Convenient- Same optimized protocol for
different sources of sample
æ High yield- Recovers 2 µg total gDNA and 0.7 µg total
RNA from 200 µl Fresh Blood sample
æ Reproducible- Delivers almost equal amount of yieldon
every isolation
æ Easy to use- No requirement of addition of Proteinase K
externally
æ Cost effective – More preps for the money
æ EcoFriendly- Minimum number of steps, thus minimum
number of plasticware required
Fig. 1.
Total nucleic acid was isolated from 4 different individual (S1-S4).
DNA and RNA were extracted from 1ml of bood. 1/10th volume of
isolated DNA and RNA were run in 1% agarose gel
DNA RNA
S1 S2 S3 S4 S1 S2 S3 S4
Cat. # Product Pack Size
GRD1003BGSure® Ultra Nucleic Acid
Isolation Kit (Blood)20 prep
GRD1003 GSure® Ultra Nucleic Acid
Isolation Kit (Blood)
50 prep
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GCC Biotech (India) Pvt. Ltd. | tech.support@gccbiotech.co.in | 91-33-24951044/24950004 | gccbiotech.co.in
GSure® Ultra Nucleic Acid Isolation Kit (Tissue)
Description
Proper lysis of tissue is the step to isolate good quantity and
quality gDNA and RNA. GSure® Ultra Nucleic acid isolation kit
(Tissue) contains 3 lysis buffers which lyse the tissue samples as
fast and efficient as possible. For complete isolation of DNA and
RNA from tissue, only 15 min incubation of the sample in lysis
buffer is enough. The kit is highly efficient to deliver total RNA
from animal tissue viz. organs, muscle, skin or even from tail.
Optimized protocol guaranties extremely purified DNA and
RNA from same sample. GSure® Ultra Nucleic acid isolation kit
(Tissue) is so robust that it can isolate DNA and RNA from any
type of tissue samples ensuring same yield every time. Proper
sample preparation is vital to obtain high yield of DNA and RNA.
Advantages
ü Highly Purified DNA and RNA from Tissue Just in 45 min.
ü For purification of genomic DNA and total RNA from
animal tissue viz. organs,
ü muscle, skin.
ü An easy to use silica membrane, spin-mini-column based
DNA-RNA isolation kit
ü for rapid, robust and reproducible isolation of genomic
DNA and total RNA from same samples.
ü Required sample volume is less and easy workflow.
ü Equally efficient on fresh as well as stored tissue sample in
NA Later
ü Delivers high quality integrated RNA in every isolation.
ü Doesn't retain impurities from sample in the eluted DNA
and RNA.
Ordering Information
Features
æ Easy – spin column format.
æ Convenient- Same optimized protocol for different
sources of sample.
æ High yield- Recovers 2 µg total gDNA and 4µg total RNA
from 10 mg mice brain.
æ Reproducible-Delivers almost equal amount of yield on
every isolation.
æ Easy to use- No requirement of addition of Proteinase K
externally.
æ Cost effective – More preps for the money.
æ EcoFriendly- Minimum number of steps, thus minimum
number of plasticware required.
Fig. 1.
Isolation of DNA and RNA from different tissue of mice using GSure®
Ulra Nucleic acid Isolation Kit for Tissue: DNA and RNA were isolated
from 10 mg of corresponding tissue samples of Mice. 1/10th volume of
isolated nucleic acid (DNA and RNA) were analyzed in 1% agarose gel.
DNA RNA
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Cat. # Product Pack Size
GRD1005B GSure® Ultra Nucleic Acid
Isolation Kit (Tissue)20 prep
GRD1005 GSure® Ultra Nucleic Acid
Isolation Kit (Tissue)
50 prep
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
GCC Biotech (India) Pvt. Ltd. | tech.support@gccbiotech.co.in | 91-33-24951044/24950004 | gccbiotech.co.in 58
GSure® Ultra Nucleic Acid Isolation Kit (Cultured cells)
Description
Gsure® Ultra Nucleic acid isolation kit (Cultured Cell) is
designed and optimized to isolate gDNA and total RNA from
cultured cells. The kit works equally efficient on adherent as well
as suspension cells. Isolated RNA is remarkably enriched with
small RNA. This kit can isolate nucleic acids from a wide range of
sample volume (10,000 cells to 6 million cells). Lysis buffers is
composed of Proteinase K, so external addition of the enzyme is
not required. Isolated DNA and RNA are compatible for all sorts
of downstream applications like restriction digestion, PCR,
Southern blotting or next generation sequencing with the
isolated DNA. Northern blotting experiments, RT-PCR, RNase
Protection Assay or the micro array could be done with the
isolated RNA.
Advantages
ü Highly Purified DNA and RNA from cultured Cell Just in
45 min.
ü For purification of genomic DNA and total RNA from 6
million cultured cells.
ü An easy to use silica membrane, spin-mini-column based
DNA-RNA isolation kit for rapid, robust and reproducible
isolation of genomic DNA and total RNA fro same
samples.
ü Required sample volume is less and easy workflow.
ü Equally efficient on different cell lines.
ü Delivers high quality integrated RNA in every isolation.
ü Doesn't retain impurities from sample in the eluted DNA
and RNA.
Ordering Information
Features
æ Fast – less than one hour required
æ Easy – spin column format
æ Convenient- Same optimized protocol for different
sources of sample
æ High yield- Recovers 10µg total gDNA and 45µg total
RNA from 6 million Cultured Cell
æ Reproducible- Delivers almost equal amount of yieldon
every isolation
æ Easy to use- No requirement of addition of Proteinase K
externally
æ Cost effective – More preps for the money
æ EcoFriendly- Minimum number of steps, thus minimum
number of plasticware required
Fig. 1:
DNA and RNA were isolated from different cell lines. 10% volume of
isolated nucleic asids were run on 1% agarose gel.
DNA RNA
Cat. # Product Pack Size
GRD1002B GSure® Ultra Nucleic Acid
Isolation Kit (Cell Culture)20 prep
GRD1002 GSure® Ultra Nucleic Acid
Isolation Kit (Cell Culture)
50 prep
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Description
Plant cells contain cell wall which is very hard to break and
isolation of gDNA and RNA from plant tissues is always a great
challenge. Usually plant tissues are crushed with liquid nitrogen
followed by isolation procedure. GSure® Ultra Nucleic acid
isolation kit (Plant) comes with a pre patented unique and
efficient buffer composition which does not require the use of
liquid nitrogen prior isolation, thus making the kit more useful.
GSure® Ultra Nucleic acid isolation kit (Plant) is highly efficient
to deliver total RNA from wide range of samples like Plant Root,
leaf, Seed, Flower and Fruit. Purified DNA and RNA are free from
any sort of secondary metabolites and are compatible for all
kind of downstream applications. Sample requirement of the kit
is only 25�mg for leaf samples and the yield of DNA is not less
than 2.5 mg for DNA and 10mg for RNA.
Advantages
ü Highly Purified DNA and RNA from any sample Just in 45
min.
ü For purification of genomic DNA and total RNA from Plant
tissue, animal tissue,cultured cells, blood and bacteria.
ü An easy to use silica membrane, spin-mini-column based
DNA-RNA isolation kit
ü for rapid, robust and reproducible isolation of genomic
DNA and total RNA from same samples.
ü Required sample volume is less and easy workflow.
ü Equally efficient on fresh as well as stored sample in RNA
Later.
ü Delivers high quality integrated RNA in every isolation.
ü Doesn't retain impurities from sample in the eluted DNA
and RNA.
Fig. 1.
Isolation of DNA and RNA were isolated from 5 different plant leafs
(P1-P5) (25mg each) electrophoresed on 1% Agarose gel.
Features
æ Fast – less than one hour required.
æ Easy – spin column format.
æ Convenient- Same optimized protocol for different
sources of sample.
æ High yield- Recovers 2 µg total gDNA and 2 µg total RNA
from 25 mg Plant Leaf Sample.
æ Reproducible-Delivers almost equal amount of yield on
every isolation.
æ Easy to use- No requirement of addition of Proteinase K
externally.
æ Cost effective – More preps for the money.
æ EcoFriendly- Minimum number of steps, thus minimum
number of plasticware required.
Fig. 2.
DNA and RNA were isolated from 100mg of stored sapota seed.
1/10th volume of the eluted DNA and RNA were fractionated on 1%
agarose gel.
Ordering Information
GSure® Ultra Nucleic Acid Isolation Kit (Plant)
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DNA RNA
Cat. # Product Pack Size
GRD1004BGSure® Ultra Nucleic Acid
Isolation Kit (Plant)20 prep
GRD1004 GSure® Ultra Nucleic Acid
Isolation Kit (Plant)
50 prep
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
GCC Biotech (India) Pvt. Ltd. | tech.support@gccbiotech.co.in | 91-33-24951044/24950004 | gccbiotech.co.in 60
GSure® Dogma kit is highly efficient to deliver high amount of
genomic DNA, RNA and protein from same sample. This kit
provides a simple and convenient technique to isolate all three
biomolecules from wide range of samples like Plant tissue,
animal tissue, cultured cells, blood and bacteria. Time required
for isolation of all three bio-molecules is just one hour. The kit
can isolate all three biomolecules from same sample, thus it
allows the researchers for simultaneous analysis of genomics,
transcriptomics and proteomics of a particular sample. Isolation
of DNA, RNA and Protein from same source minimizes the
experimental error and makes the data more scientific. On one
hand, the kit is cost-effective as it offers more preps for money
and on the other hand, it is eco-friendly since it minimizes the
use of plastic wares. GSure® Dogma Kit comes with such buffer
composition that isolation protocol for different sources are
same. Sample Volume required for isolation is also minimal and
isolation steps are simple and easy.
Gsure® Dogma kit combines the advantages of a silica-based
system with a microspin format, thus eliminates the requirement
of expensive resins and hazardous organic compounds. GSure
Dogma kit comes with Gmini DNA Spin column which is
specially designed to bind genomic DNA. This kit also contains
GMini CHROME column which is specialized for binding total
RNA selectively. The DNA spin columns as well as the Chrome
columns retain minimum amount alcohol from wash buffer;
hence delivers purified form of genomic DNA and total RNA
every time after elution.
Buffers provided with the GSure Dogma Kit assure complete
lysis of cells without digestion of cellular proteins. Buffer
composition is optimized in such a way that DNA binds
selectively to GMini Spin column and RNA binds selectively to
the GMini CHROME column whereas the total protein comes in
the flow-through. Dogma kit also comes with a unique Protein
Precipitation Cocktail for rapid precipitation of total protein
from the flow-through. Protein pellet wash buffer ensures
complete removal of contaminants and delivery of purified
protein pellet every time. Protein pellet resuspension solution
ensures complete dissolution of pellet.
Purified genomic DNA is compatible for all sorts of downstream
applications like PCR amplification, southern blotting,
restriction digestions, next generation sequencing etc. Purified
RNA is compatible for RT-PCR amplification, northern blotting,
RNase Protection Assay, Microarray etc. Purified protein is
compatible for total protein profiling, western blotting etc. ern
blotting, RNase Protection assay and microarray etc.
Description
GSure® Dogma Kit
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
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Advantages
ü Complete Isolation from Same Sample just in 1 Hr.
ü Fast and efficient purification of DNA, RNA and Protein
from 500 µl bacterial cells.
ü An easy to use silica membrane, spin-mini-column based
isolation kit for rapid, robust and reproducible isolation of
all three bio-molecules.
ü Required sample volume is less and easy workflow.
ü Delivers high quality integrated DNA, RNA and Protein in
every isolation.
ü Doesn't retain impurities from sample in the eluted DNA
and RNA.
ü Qualitatively and quantitatively better than any Phase
Separation Method
Fig.1.
Over expression followed by isolation of DNA, RNA and Protein from
same sample at different time points.
Fig.2.
RT PCR was performed RNA on total was RNA from H2BpET28b
transformed BL21DE3, RNA was harvested before and after isolation.
Features
æ Efficient- DNA, RNA and Protein from same sample.
æ Fast– One hour required to isolate 3 bio- molecules.
æ Convenient- Same optimized protocol for different
sources of sample
æ High yield- Recovers 5 µg DNA, 25 µg RNA and 50 µg
Protein from 500 µl bacterial cells.
æ User Friendly– Less sample volume and easy
æ workflow.
æ Reproducible- Delivers almost equal amount of
æ yield on every isolation.
æ Easy– spin column format.
æ Cost effective- More preps for the money.
æ Eco-Friendly- Minimum number of steps, thus
æ minimum number of plastic ware required.
Fig. 3.
Bar Plot showing expression profile of H2B protein at different time
after induction with IPTG. Expression profiling was performed at RNA
level by RT PCR and at protein level by target band quantification.
Ordering Information
GSure® Dogma Kit for Bacteria
Cat # Product Pack Size
GD1001AGSure® Dogma Kit for
Bacteria20 prep
GD1001GSure® Dogma Kit for
Bacteria50 prep
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
GCC Biotech (India) Pvt. Ltd. | tech.support@gccbiotech.co.in | 91-33-24951044/24950004 | gccbiotech.co.in 62
Advantagesü Complete Isolation from Plant Sample just in 1 Hr.
ü Fast and efficient purification of DNA, RNA and Protein
from Plant parts viz.
ü Root, leaf, Seed, Flower and Fruit.
ü An easy to use silica membrane, spin-mini-column based
isolation kit for rapid.
ü robust and reproducible isolation of all three bio-
molecules.
ü Required sample volume is less and easy workflow.
ü Delivers high quality integrated DNA, RNA and Protein in
every isolation.
ü Doesn't retain impurities from sample in the eluted DNA
and RNA.
ü Qualitatively and quantitatively better than any Phase
Separation Method.
Fig.1.
DNA, RNA and protein were isolated from 5 differentaged Colocasia
leafs (25mg). 10% of the isolated biomolecules were electrophoresed on
gel.
Featuresæ Efficient- DNA, RNA and Protein from same sample.
æ Fast– One hour required to isolate 3 bio-molecules.
æ Convenient- Same optimized protocol for different
sources of sample
æ High yield- Recovers 3µg DNA, 3µg RNA and 50µg
Protein from 25mg plant leaf.
æ User Friendly– Less sample volume and easyworkflow.
æ Reproducible- Delivers almost equal amount of yield on
every isolation.
æ Easy – spin column format.
æ Cost effective – More preps for the money.
æ Eco-Friendly- Minimum number of steps, thus minimum
number of plasticware required.
Ordering Information
GSure® Dogma Kit for Plant
Cat. # Product Pack Size
GD1002A GSure® Dogma Kit for Plant 20 prep
GD1002 GSure® Dogma Kit for Plant 50 prep
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
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Ultrapure Oligonucleotides
GCC provides a full spectrum of highquality oligonucleotides modifications,with various synthesis scales and applications to ensure consistentlyfast turnaround time.
Ultrapure Oligonucleotides
GCC provides a full spectrum of highquality oligonucleotides modifications,with various synthesis scales and applications to ensure consistentlyfast turnaround time.
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GCC Biotech (India) Pvt. Ltd. | tech.support@gccbiotech.co.in | 91-33-24951044/24950004 | gccbiotech.co.in
Advantageü Complete Isolation from Cultured Cell just in 1 Hr.
ü Fast and efficient purification of DNA, RNA and Protein
from 1 million cultured cells.
ü An easy to use silica membrane, spin-mini-column based
isolation kit for rapid,robust and reproducible isolation of
all three bio-molecules.
ü Required sample volume is less and easy workflow.
ü Delivers high quality integrated DNA, RNA and Protein in
every isolation.
ü Doesn't retain impurities from sample in the eluted DNA
and RNA.
ü Qualitatively and quantitatively better than any Phase
Separation Method.
Fig. 1.
DNA, RNA and Protein were isolated from different cultured cell line.
DNA and RNA were run on 1% agarose TAE gel. l/10th volume of isolated
biomolecules were fractionated on gel.
Featuresæ Efficient- DNA, RNA and Protein from same sample.
æ Fast– One hour required to isolate 3 bio-molecules.
æ Convenient- Same optimized protocol fordifferent sources
of sample
æ High yield- Recovers 5 µg DNA, 25 µg RNA and 50 µg
Protein from 1 million cultured cells.
æ User Friendly– Less sample volume and easy workflow.
æ Reproducible- Delivers almost equal amount of
æ yield on every isolation.
æ Easy– spin column format.
æ Cost effective– More preps for the money.
æ Eco-Friendly- Minimum number of steps, thus minimum
number of plasticware required.
Ordering Information
GSure® Dogma Kit for Cultured Cell
Cat. # Product Pack Size
GD1003AGSure® Dogma Kit for Cultured Cell
20 prep
GD1003 Gsure® Dogma Kit for Cultured Cell 50 prep
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
Tailored to Meet Your Needs....
GCC’s popular protein biology products
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for protein purification
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GCC Biotech (India) Pvt. Ltd. | tech.support@gccbiotech.co.in | 91-33-24951044/24950004 | gccbiotech.co.in 64
GSure® Dogma Kit for Tissue
Advantages
ü Complete Isolation from Tissue Sample just in 1 Hr.
ü Fast and efficient purification of DNA, RNA and Protein
from Plant parts viz. Root, Leaf, Seed, Flower and Fruit.
ü An easy to use silica membrane, spin-mini-column based
isolation kit for rapid, robust and reproducible isolation of
all three bio-molecules.
ü Required sample volume is less and easy workflow.
ü Delivers high quality integrated DNA, RNA and Protein in
every isolation.
ü Doesn't retain impurities from sample in the eluted DNA
and RNA.
ü Qualitatively and quantitatively better than any Phase
Separation Method.
Fig. 1:
Isolation of DNA, RNA and Protein from different tissue of mice using
GSure® Dogma Kit for Tissue: DNA, RNA and Protein were isolated from
lOmg of corresponding tissue samples of Mice. Mice was sacrifised and
the organs were collected in RNA Later. Biomolecules were extracted
after 1 week of tissue collection. l/10th volume of isolated nucleic acid
(DNA and RNA) and protein were fractionated on 1% agarose TAE and
12% SDS-Polyacrylamide gel respectively.
Features
æ Efficient- DNA, RNA and Protein from same sample.
æ Fast– One hour required to isolate 3 bio-molecules.
æ Convenient- Same optimized protocol for different
sources of sample
æ High yield- Recovers 3 µg DNA, 3 µg RNA and 50 µg
Protein from 10 mg mice brain.
æ User Friendly– Less sample volume and easy workflow.
æ Reproducible- Delivers almost equal amount of yield on
every isolation.
æ Easy– spin column format.
æ Cost effective– More preps for the money.
æ Eco-Friendly- Minimum number of steps, thus minimum
number of plastic ware required.
Ordering Information
Cat. # Product Pack Size
GD1004A GSure® Dogma Kit for Tissue 20 prep
GD1004 GSure® Dogma Kit for Tissue 50 prep
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GCC Biotech (India) Pvt. Ltd. | tech.support@gccbiotech.co.in | 91-33-24951044/24950004 | gccbiotech.co.in
Advantages
ü Complete Isolation from 200ul Blood Sample just in 1 Hr.
ü Fast and efficient purification of DNA, RNA and Protein
from 200 µl to 1ml Fresh Blood.
ü An easy to use silica membrane, spin-mini-column based
isolation kit for rapid, robust and reproducible isolation of
all three bio-molecules.
ü Required sample volume is less and easy workflow.
ü Delivers high quality integrated DNA, RNA and Protein in
every isolation.
ü Doesn't retain impurities from sample in the eluted DNA
and RNA.
ü Qualitatively and quantitatively better than any Phase
Separation Method.
Fig. 1:
DNA RNA and Protein were isolated from 1 ml of blood of 4 different
individuals.10% of the isolated biomolecules were electrophoresed on
gel. DNA and RNA were run on 1% Agarose TAE gel.
Fig. 2:
Total RNA was isolated from 1ml blood from individuals. RNA was
eluted in 50ml water and 1/10th fraction is loaded on 1% agarose gel.
Features
æ Efficient- DNA, RNA and Protein from same sample.
æ Fast– One hour required to isolate 3 bio-molecules
æ Convenient- Same optimized protocol for different
sources of sample.
æ High yield- Recovers 5µg DNA, 2µg RNA and 50µg
Protein from 200µl Fresh Blood.
æ User Friendly– Less sample volume and easy workflow
æ Reproducible- Delivers almost equal amount of yield on
every isolation
æ Easy – spin column format.
æ Cost effective– More preps for the money
æ Eco-Friendly- Minimum number of steps, thus minimum
number of plasticware required.
Fig. 3:
DNA was Isolated from 100pJ of whole blood, serum and WBC. 1/10th
volume was electrophored on 1% agarose gel..
Ordering Information
GSure® Dogma Kit for Blood
Cat # Product Pack Size
GD1005A GSure®Dogma Kit for Blood 20 prep
GD1005 GSure®Dogma Kit for Blood 50 prep
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GCC Biotech (India) Pvt. Ltd. | tech.support@gccbiotech.co.in | 91-33-24951044/24950004 | gccbiotech.co.in 66
Description
GCC Biotech offers One Step RT-PCR Kit for sensitive and end
point detection of RNA templates. One Step RT-PCR Kit
includes dNTPS, one step 10 X reaction buffer, enzyme mix
with reverse transcriptase and proof reading hot start DNA
polymerase and ribonuclease inhibitor.
Storage
-20°C to -10°C in a non-frost-free freezer. Guaranteed stable
for 6 months when stored properly. Repeatative freeze-thaw
cycles should be minimized.
Fig. 1:
PCR amplification using 1 Step RT-PCR Kit from in vitro transcript RNA.
Lane M: molecular size marker -1kb DNA Ladder. Lanes A1 & A2 (1Kb),
B1 &B2 (1.5Kb), C1 & C2 (2Kb): PCR amplification reactions, using 10 x
One step RT Buffer with 0.2 mM dNTPs and 1U enzyme mix in 20 ml
reaction volume. 5 ml loaded for analysis in 1% agarose gel.
Features
æ cDNA synthesis and PCR amplification steps are
performed in a single reaction using gene specific primers
æ It is fast and easy one tube set up
æ Results in high-yield amplification with minimal
optimization
æ It can be used for any type of RNA template.
æ It is recommended in detection or quantification of
several mRNA from, a single step.
Kit Components
One Step RT-PCR Kit
G9 Taq DNA polymerase
Ÿ An ideal tool for standard PCR of templates of 6kb and shorter
Ÿ High throughput PCR from complex genomic, viral, and plasmid
Ÿ templates compatible for TA cloning and RT-PCR
Kit Component Taq (2.5 unit//ul); 10XTaq Buffer; 25 mM, MgCI2;
Control DNA template; Control Primer
Kit Size : 500U; 2X 500U; 10X500U
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
Component G7113 G7113A
Enzyme Mix 1
Enzyme Mix 2
10X One-Step Reaction buffer
100mM MgCl 2
dNTP mix, 10 mM
Nuclease-free H O 2
Control RNA Template ( 300 ng,3Rxn)
Control Forward primer (10 mM)
Control Reverse primer (10 mM)
50 µl
50 µl
100 µl
25 µl
50 µl
1.5 ml
3 µl
3 µl
3 µl
100 µl
100 µl
200 µl
50 µl
100 µl
1.5 ml
3 µl
3 µl
3 µl
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Description
GCC Biotech offers Dual Step RT-PCR Kit for fast cDNA synthesis
enabling two-step RT-PCR for gene expression analysis. In the
first step, M-MulV Reverse Transcriptase (RT) is used with
random primer, oligodT primer or gene specific primer annealed
to an RNA sample. In the second step, PCR amplification is
performed in a separate tube using gene specific primers. A
ready to useTaq 2X PCR Master Mix is provided for its
convenient and consistent amplification performance.
Storage
-20°C to -10°C in a non-frost-free freezer. Guaranteed stable
for 6 months when stored properly. Repetitive freeze-thaw
cycles should be minimized.
Fig.1.
PCR amplification using Dual Step RT-PCR Kit from Diffrent RNA Sources
Lane M: molecular size marker - Perfect 1kb DNA Ladder. Lanes 1
L(3kb),RNA from plant RNA and L2 RNA from animal cell line. cDNA
prepared using M-MulV Revers Transcriptase, 10x RT buffer dNTPs and
1U enzyme mix in 20 ml reaction volume.
Features
æ High cDNA yields even from low-abundance transcripts
æ Multiple transcripts can be detected from asingle first
strand cDNA synthesis
æ Semi quantitative analysis of the mRNA level can be
achieved by agarose gel electrophoresis.
Kit Components
Dual Step RT-PCR Kit
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LGquant�Pico �Green�dsDNA�Quantitation�Reagent
£ An ultra-sensitive fluorescent nucleic acid stain
£ 10,000 times more sensitive than UV absorbance based measurements
£ Many applications in molecular biological procedures like cDNA synthesis for library production, DNA fragment purification for subcloning, as well as diagnostic applications
Component G7114 G7114A
M-MLV Reverse Transcriptase
(M-MLVRT) (200u/µl)
10X M-MLV RT Buffer with MgCl2
10 mM dNTP mix
2X Hi G9 Taq PCR Master Mix
Sterile, DEPC water
0.1 ml
0.2 ml
0.1 ml
1.25 ml X 2
1.5 ml
0.2 ml
0.4 ml
0.2 ml
1.25 ml X 4
1.5 ml
GCC Biotech (India) Pvt. Ltd. | tech.support@gccbiotech.co.in | 91-33-24951044/24950004 | gccbiotech.co.in 68
Description
G9 Taq DNA Polymerase is a thermostable recombinant DNA
polymerase, expressed and purified from E.coli. G9 polymerase
catalyzes the polymerization of nucleotides into duplex DNA in
5'- 3' direction in the presence of magnesium ions , has no
detectable 3' 5' exonuclease (proofreading) activity and →
possesses low 5' 3' exonuclease activity. G9 Taq DNA →
polymerase supplied with a special and improved buffer system
that increases thermostability (enzyme half-life) and
processivity of Taq Polymerase by stabilizing the enzyme during
PCR. G9 Taq DNA Polymerase buffer is a combination of
different additives, which protects enzymes under stress
conditions such as desiccation, heat, and changes in pH and salt
concentration. G9 Taq polymerase shows a robust amplification
of templates even with higher complexity.
Applications
l High throughput PCR from complex genomic, viral,
and plasmid templates
l TA Cloning
l RT-PCR.
Unit Definition
One unit of G9 Taq DNA Polymerase is the amount of enzyme
required to incorporate 10 nmoles of deoxyribonucleotide
into DNA in 30 min at 74°C material in 30 min at 74°C.
Storage
-20°C to -10°C in a non-frost-free freezer. Guaranteed stable
for 2 years when stored properly. Avoid repeated
freeze/thawing of reagents.
Kit Components
Features
æ High cDNA yields even from low-abundance transcripts
æ Multiple transcripts can be detected from a single first
strand cDNA synthesis
æ Semi quantitative analysis of the mRNA level can be
achieved by agarose gel electrophoresis
æ PCR for higher fragment upto 6 kb.
Fig. 1: PCR amplification using G9 Taq DNA Polymerase and
Other Brand’s Taq polymerase.
Lane M: molecular size marker - 1 kb DNA Ladder. A comparative 4
and 6 kb PCR amplification reactions, using respective 10 X reaction
Buffer with 0.2 mM dNTPs and 1.25 U respective DNA polymerase in
50 μl reaction volume. 5 μl loaded for analysis in 1% agarose gel.
G9 Taq DNA Polymerase
Component G7115B G7115 G7115A
G9 Taq DNA Polymerase (2.5 unit//l)
10X Taq Reaction Buffer
25 mM MgCl2
Control DNA template
Control Primer
500 Unit
(2 X 1.25 ml)
(2 X 1.25 ml)
10�ml
10�ml
2X500 Unit
(2 X 1.25 ml)
(2 X 1.25 ml)
10�ml
10�ml
10X500 Unit
(20 X 1.25 ml)
(20 X 1.25 ml)
10�ml
10�ml
M G9
Taq
Oth
er B
ran
d
G9
Taq
6 kb
4 kb
Oth
er B
ran
d
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Description
Hi-G9 Taq DNA Polymerase is a thermostable recombinant
DNA polymerase, expressed and purified from E.coli. Like G9
polymerase this polymerase catalyzes the polymerization of
nucleotides into duplex DNA in 5'- 3' direction in the presence of
magnesium ions , has no detectable 3' 5' exonuclease →
(proofreading) activity and possesses low 5' 3' exonuclease →
activity. Hi-G9 Taq DNA polymerase is G9 Taq DNA polymerase
supplied with a special and improved buffer system that
increases thermostability (enzyme half-life) and processivity of
Taq Polymerase by stabilizing the enzyme during PCR. Hi-G9
Taq DNA Polymerase buffer is a combination of different
additives, which protects enzymes under stress conditions such
as desiccation, heat, and changes in pH and salt concentration.
Hi-G9 Taq polymerase shows a robust amplification of
templates even with higher complexity.
Applications
l High throughput PCR from complex genomic, viral,
and plasmid templates
l TA cloning
l PCR for high GC content amplicons.
l RT-PCR
Unit Definition
One unit of Hi-G9 Taq DNA Polymerase is the amount of
enzyme required to incorporate 10 nmoles of
deoxyribonucleotide into DNA in 30 min at 74°C.
Storage
-20°C to -10°C in a non-frost-free freezer. Guaranteed stable
for 2 years when stored properly. Avoid repeated
freeze/thawing of reagents.
Kit Components
Features
æ Improved sensitivity, specificity High yields
æ Unique buffer formulation facilitates high throughput PCR
æ PCR for higher fragment upto 8 kb
æ Generates PCR products with 3'-dA overhangs.
Fig. 1:
PCR amplification using Hi-G9 Taq DNA 1st Lane: molecular size
marker - 1 kb DNA Ladder. 2nd and 3rd lane : 8 kb PCR amplification
reactions, using 10 X Hi-G9 taq reaction Buffer with 0.2 mM dNTPs
and 1.25 U Hi-G9 taq DNA polymerase in 50 μl reaction volume. 2 ml
loaded for analysis in 1% agarose gel.
Fig. 2.
Amplification of 200bp fragments from GC rich template (50%, 60%,
70% and 80% respectively) with Hi-G9 taq polymerase in presence of
GCfiX buffer.
Hi-G9 Taq DNA Polymerase
8kb
1 kb
la
dd
er
Percent of GC content50% 60% 70% 80%1
00 b
p
lad
der
Component GG03 GG01 GG02
Hi-G9 Taq DNA Polymerase (2.5 unit/λ)
10X Hi-G9 Taq Reaction Buffer
25 mM MgCl2
Control DNA template
Control Primer mix
500 Unit
(2 X 1.25 ml)
(2 X 1.25 ml)
10 µl
10 µl
(2X 500 Unit)
(4 X 1.25 ml)
(4 X 1.25 ml)
10 µl
10 µl
(10X 500 Unit)
(20 X 1.25 ml)
(20 X 1.25 ml)
10 µl
10 µl
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GCC Biotech (India) Pvt. Ltd. | tech.support@gccbiotech.co.in | 91-33-24951044/24950004 | gccbiotech.co.in 70
Description
Prime Taq DNA Polymerase is an optimized combination of G9
Taq DNA polymerase and high fidelity DNA polymerases from
Pyrococcus species for use in routine and difficult PCR
experiments. The 3´ 5´ exonuclease activity of the high fidelity →
DNA Polymerase increases the fidelity and robustness in
amplification by Taq DNA Polymerase, even from very low copy
number of template. The 10 X Reaction Buffer has also been
formulated for robust yield of desirable PCR products with
requirement of minimal optimization. Prime Taq DNA
polymerase is capable of amplifying amplicons up to 10kb.
Prime Taq DNA polymerase has improved efficiency of
polymerization irrespective of templates having high GC
content and contamination of exogenous PCR inhibitor. Prime
Taq DNA Polymerase is supplied with 10X Prime Taq Standard
Reaction Buffer to provide robust amplification.
Applications
l High throughput Long range PCR from complex
genomic, viral, and plasmid templates (up to 10 kb)
l Colony PCR
l GC-rich PCR
l AT-rich PCR
l Routine use PCR
l RT-PCR
Unit Definition
One unit of Prime Taq DNA Polymerase is the amount of
enzyme required to incorporate 10 nmoles of
deoxyribonucleotide into DNA in 30 min at 74°C.
Storage
-20°C to -10°C in a non-frost-free freezer. Guaranteed stable
for 2 years when stored properly. Avoid repeated freeze
/thawing of reagents.
Kit Components
Features
æ A perfect blend of two different DNA polymerases for the
robust yield in PCR reaction of higher sized fragments.
æ Unique buffer formulation to facilitate improved
sensitivity, specificity, and yields for long range
æ Robust yield of long amplicons (up to 10 kb) as compared
to any Taq DNA polymerase.
Fig.1.
Amplification of differnt fegions from lamda DNA with Prime Taq DNA
polymerase, 10µl of PCR product were loded on 1% agarose gel.
Prime Taq DNA Polymerase
Component G4798 G4798A G4799
Prime Taq DNA Polymerase (2.5 unit/ml)
10X Prime Taq Reaction Buffer
25 mM MgCI2
Control DNA template
Control Primer mix
500 Unit
(2 X 1.25 ml)
(2 X 1.25 ml)
10 µl
10 µl
(2X500 Unit )
(2 X 1.25 ml)
(2 X 1.25 ml)
10 µl
10 µl
(10X500 Unit )
(20 X 1.25 ml)
(20 X 1.25 ml)
10 µl
10 µl
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Description
Hi-Prime Taq DNA Polymerase is an optimized blend of G9 Taq
DNA polymerase and high fidelity DNA polymerases from
Pyrococcus species in presence of specially formulated buffer
which supports PCR amplification from critical amplicons.
Presence of high fidelity DNA polymerases from Pyrococcus
species in optimized ratio and the enhancer buffer system make
Hi-Prime Taq DNA polymerase an excellent choice for PCR
amplification from natural isolates. The 3´ 5´ exonuclease →
activity of the high fidelity DNA Polymerase increases the fidelity
and robustness in amplification by Taq DNA Polymerase, even
from very low copy number of template.
Hi-Prime Taq DNA polymerase comes with a specially
formulated buffer, which allows amplification up to 12kb or
more.
Applications
l PCR from natural isolates.
l PCR for diagnostic samples.
l PCR for forensic samples.
l PCR for high GC content amplicons.
l PCR for soil DNA
Unit Definition
One unit of Hi Prime Taq DNA Polymerase is the amount of
enzyme required to incorporate 10 nmoles of
deoxyribonucleotide into DNA in 30 min at 74°C.
Storage
-20°C to -10°C in a non-frost-free freezer. Guaranteed stable
for 2 years when stored properly. Avoid repeated freeze
/thawing of reagents.
Kit Components
Features
æ A perfect blend of two different DNA polymerases
æ for the robust yield in PCR reaction of higher sized
fragments
æ Unique buffer formulation to facilitate improved
æ sensitivity, specificity, and yields for long range
æ PCR ( ≥ 12 Kb)
Fig.1.
PCR amplification using Hi-Prime Taq DNA 1st Lane: molecular size
marker - 1 kb DNA Ladder. 2nd and 3rd lane: 12 kb PCR amplification
reactions, using 10X Prime Taq reaction Buffer with 0.2 mM dNTPs
and 1.25 U respective DNA polymerase in 50 μl reaction volume. 5 ml
loaded for analysis.in 1% agarose gel.
Hi-Prime Taq DNA Polymerase
Component G7116 G7116A G7116B
Hi-Prime Taq DNA Polymerase (2.5 unit/λ)
10X Hi-Prime Taq Reaction Buffer
25 mM MgCl2
Control DNA template
Control Primer mix
500 Unit
(2 X 1.25 ml)
(2 X 1.25 ml)
10 µl
10 µl
2X 500 Unit
(4 X 1.25 ml)
(4 X 1.25 ml)
10 µl
10 µl
10X 500 Unit
(20 X 1.25 ml)
(20 X 1.25 ml)
10 µl
10 µl
12 kb
1 k
b
lad
der
Heat Inactivation- No
3' to 5' Exonuclease activity- Yes
5' to 3' Exonuclease activity- Yes
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
GCC Biotech (India) Pvt. Ltd. | tech.support@gccbiotech.co.in | 91-33-24951044/24950004 | gccbiotech.co.in 72
Description
G9 Taq DNA Polymerase is a thermostable DNA polymerase
having polymerase activity in 5'- 3' direction and and a 5´ flap
endonuclease activity.This recombinant protein was expressed
and purified from E.coli. The G9 2X Master Mix is an an
optimized ready-to-use solution containing Taq DNA
Polymerase, dNTPs, MgCl2 , KCI and stabilizers, requiring only
the addition of primers and DNA template for desired
amplification.
Applications
l PCR from wide range of natural isolates.
l Long PCR
l TA cloning
l Primer Extension
Unit Definition
One unit of PrimeTaq DNA Polymerase is the amount of
enzyme mix required to incorporate 10 nmoles of
deoxyribonucleotide into DNA in 30 min at 74°C.
Storage
-20°C to -10°C in a non-frost-free freezer. Guaranteed stable
for 2 years when stored properly.Avoid repeated
freeze/thawing of reagents
Kit Components
Features
æ Designed for easy and regular use
æ Half-life is more than 40 min at 95°C
æ Generates PCR products with 3'-dA overhangs
æ It can amplify up to 6 kb from lambda DNA.
Fig.1.
PCR amplification with 2X G9 Taq PCR Master Mix from lambda
genomic DNA 1st Lane: molecular size marker - 1 kb DNA Ladder. 2nd
and 3rd lane : 6 kb PCR amplification reactions, using 2X G9 Taq PCR
Master Mix in 50 ml final reaction volume. 5 ml loaded for analysis in
1% agarose gel.
2X G9 Taq PCR Master Mix
1 k
b la
dder
6 kb
With 2X Hi
G9 Taq PCR
Master Mix
Note:
ü 2X G9 Taq PCR Master Mix are recommended to use at a 1X concentration, adding DNA template and
primers in a total reaction volume of 25 or 50 μl.
ü 2X G9 Taq PCR Master Mix is stable for 25-30 freeze-thaw cycles when stored at -20 ° C.
ü In some complicated PCR, further MgCl need to be incorporated in final reaction mixture.2
Component G7117 G7117A G7117B
2x G9 Taq PCR Master Mix
Control DNA
TemplateControl Primer mix
100 reactions
10 µl
10 µl
250 reactions
10 µl
10 µl
1000 reactions
10 µl
10 µl
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Description
Hi-G9 Taq PCR Master Mix is a thermostable DNA polymerase
having polymerase activity in 5'- 3' direction and and a 5´ flap
endonuclease activity. This recombinant protein was expressed
and purified from E.coli. 2x Hi G9 Taq PCR Master Mix is a robust
Taq master mix supplied with a buffer which is an optimized,
ready-to-use DNA Polymerases ideally suited to PCR
applications from complex genomic template bacterial colonies
and cDNA products. This convenient quick-load master mix
formulation contains dNTPs and different additive buffer
components to enhance processivity of G9 Taq Polymerase.
Applications
l Colony PCR
l Long PCR
l TA cloning
l Primer Extension
Unit Definition
One unit of Hi-G9 Taq DNA Polymerase is the amount of
enzyme required to incorporate 10 nmoles of deoxyribo
nucleotide into DNA in 30 min at 74°C in its buffer optimized
condition.
Storage
-20°C to -10°C in a non-frost-free freezer. Guaranteed stable
for 2 years when stored properly. 2X HiG9 Taq PCR Master
Mix is stable for 25-30 freeze-thaw cycles when stored at -
20°C. Avoid repeated freeze/thawing of reagents
Kit Components
Features
æ Easy to use for long range PCR
æ For sensitive high throughput PCR
æ Tolerates a wide range of genomic DNA templates
æ Generates PCR products with 3'-dA overhangs
æ It can amplify up to 8 kb from lambda DNA.
Fig. 1:
PCR amplification with 2X Hi G9 Taq PCR Master Mix from lambda
genomic DNA .1st Lane: molecular size marker - 1 kb DNA Ladder.
2nd and 3rd lane : 8 kb PCR amplification reactions, using 2X Hi G9
Taq PCR Master Mix in 50 ml final reaction volume.5 ml loaded for
analysis in 1% agarose gel.
2X Hi-G9 Taq PCR Master Mix
Note:
ü 2X Hi-G9 Taq PCR Master Mix is recommended to use at a 1X concentration, adding DNA template and
primers in a total reaction volume of 25 or 50 μl.
ü 2X Hi-G9 Taq PCR Master Mix is stable for 25-30 freeze-thaw cycles when stored at -20 ° C.
ü In some complicated PCR, further MgCl2 need to be incorporated in final reaction mixture.
1 kb
lad
der
8 kb
With 2X Hi
G9 Taq PCR
Master Mix
Component G4804 G4804A G4804B
2X Hi G9 Taq PCR Master Mix
Control DNA template
Control Primer mix
100 rxn
10 µl
10 µl
250 rxn
10 µl
10 µl
1000 rxn
10 µl
10 µl
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GCC Biotech (India) Pvt. Ltd. | tech.support@gccbiotech.co.in | 91-33-24951044/24950004 | gccbiotech.co.in 74
Note:
ü 2X Prime Taq PCR Master Mix is recommended to use at a 1X concentration, adding DNA template and
primers in a total reaction volume of 25 or 50 μl.
ü 2X Prime Taq PCR Master Mix is stable for 25-30 freeze-thaw cycles when stored at -20 ° C.
ü In some complicated PCR, further MgCl2 need to be incorporated in final reaction mixture.
Description
2X Prime Taq Master Mix with Standard Buffer is an optimized
blend of Taq and Hi fidelity DNA Polymerases ideally suited to
routine PCR applications from a variety of templates including
pure DNA solutions, bacterial colonies, and cDNA products. 2X
Prime Taq Master Mix is the best choice for PCR amplification
using natural isolates as template DNA. This Hi fidelity DNA
Polymerase increases the processivity and robust amplification
of Taq DNA Polymerase. The convenient master mix formulation
contains dNTPs, MgCl . KCl buffer components and stabilizers, 2
requiring only the addition of primers and DNA template for
robust amplification.
Applications
l PCR from wide range of natural isolates.
l Long PCR
l TA cloning
l Primer Extension
Unit Definition
One unit of PrimeTaq DNA Polymerase is the amount of
enzyme mix required to incorporate 10 nmoles of
deoxyribonucleotide into DNA in 30 min at 74°C.
Storage
-20°C to -10°C in a non-frost-free freezer. Guaranteed stable
for 2 years when stored properly. 2X HiG9 Taq PCR Master
Mix is stable for 25-30 freeze-thaw cycles when stored at -
20°C.Avoid repeated freeze/thawing of reagents
Kit Components
Features
æ Easy to use for long range PCR (upto 10 kb)
æ For sensitive high throughput PCR
æ Tolerates a wide range of genomic DNA templates
Fig.1.
PCR amplification with 2X Prime Taq PCR Master Mix from lambda
genomic DNA . 1st Lane: molecular size marker - 1 kb DNA Ladder.
2nd and 3rd lane : 10 kb PCR amplification reactions, using 2X Prime
Taq PCR Master Mix in 50 ml final reaction volume. 5 ml loaded for
analysis in 1% agarose gel.
2X Prime Taq PCR Master Mix
1 kb
lad
der
10 kb
With 2X
Prime Taq PCR
Master Mix
Component G7118 G7118A G7118B
2X Prime Taq PCR Master Mix
Control DNA Template
Control Primer mix
100 rxn
10 µl
10 µl
250 rxn
10 µl
10 µl
1000 rxn
10 µl
10 µl
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Description
2X Hi-Prime Taq Master Mix with PCR enhancer cocktail is an
optimized combination of Taq and Hi fidelity DNA Polymerases
present in a buffer that is compatible to amplify from different
template source, irrespective of their GC content. This 2X Master
Mix particularly resistant to some PCR inhibitors up to a certain
percentage. The mix is suitable for direct PCR from unprocessed
samples including bacterial culture, bacterial colonies. 2X Hi
Prime Taq Master Mix can perform consistently well on a broad
range of templates (including both GC and AT rich). The
convenient master mix formulation contains dNTPs, MgCl2,
buffer components and stabilizers, requiring only the addition
of primers and DNA template for robust amplification.
Applications
l Genotyping.
l Multiplex PCR
l Library construction
l PCR from bacterial culture and urine
Unit Definition
One unit of Hi-PrimeTaq DNA Polymerase is the amount of
enzyme mix required to incorporate 10 nmoles of
deoxyribonucleotide into DNA in 30 min at 74°C
Storage
-20°C to -10°C in a non-frost-free freezer. Guaranteed
stable for 2 years when stored properly. 2X Hi-Prime
Taq PCR Master Mix is stable for 25-30 freeze-thaw
cycles when stored at -20°C. Avoid repeated
freeze/thawing of reagents.
Kit Components
Features
æ Easy to use for long range PCR (upto 12 kb)
æ For sensitive high throughput PCR
æ Tolerates a wide range of genomic DNA templates
Fig.1.
PCR amplification with 2X Hi Prime Taq PCR Master Mix from lambda
genomic DNA 1st Lane: molecular size marker - 1 kb DNA Ladder.
2nd and 3rd lane: 12 kb PCR amplification reactions, using 2X Hi-
Prime Taq PCR Master Mix in 50 ul final reaction volume. 5 ul loaded
for analysis in 1% agarose gel.
2X Hi-Prime Taq PCR Master Mix
Note:
ü 2X Hi-PrimeTaq PCR Master Mix is recommended to use at a 1X concentration, adding DNA template and
primers in a total reaction volume of 25 or 50 μl.
ü 2X Hi-Prime Taq PCR Master Mix is stable for 25-30 freeze-thaw cycles when stored at -20 ° C.
Component G7119 G7119A G7119B
2X Hi PrimeTaq PCR Master Mix
Control DNA template
Control Primer mix
100 rxn
10 µl
10 µl
250 rxn
10 µl
10 µl
1000 rxn
10 µl
10 µl
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Description
Gpfu Polymerase is a thermostable DNA polymerase isolated
from hyperthermophilic Pyrococcus furiosus. The protein is
isolated from a recombinant E.coli strain containing the gene
encoding Pfu DNA polymerase. It catalyzes the DNA-dependent
polymerization of nucleotides into duplex DNA in the 5' 3' →
direction and exhibits 3' 5' exonuclease (proof reading) →
activity. Pfu DNA polymerase is the ideal choice for a variety of
techniques requiring high-fidelity DNA synthesis by PCR
reaction. It can apply to cloning, gene expression, site-directed
mutagenesis and etc.
Applications
l High Fidelity PCR can amplify up to 4 kb
l Blunt-end PCR Cloning or mutagenesis requested
high fidelity
l Site directed mutagenesis
Unit Definition
One unit is defined as the amount of enzyme required to
catalyze the incorporation of 10nmoles of dNTP into acid
insoluble material in 30 min at 74°C.
Storage
-20 ° C to -10 ° C in a non-frost-free freezer. Guaranteed
stable for 2 years when stored properly. Avoid repeated
freeze / thawing of reagents.
Kit Components
Features
æ Easy to use for long range PCRFor sensitive high
throughput PCR
æ Tolerates a wide range of genomic DNA templates
æ Generates PCR products with 3'-dA overhangs.
Fig.1.
PCR amplification using GPfu DNA Polymerase. Lane M: molecular
size marker - 1 kb DNA Ladder. Lanes 2 to 6 kb: PCR amplification
reactions, using 10 X GPfu Buffer with 0.2 mM dNTPs and 1 U GPfu
DNA Polymerase in 50 μl reaction volume. 5μl loaded for analysis in
1% agarose gel.
Gpfu Polymerase
M 2 4 kb
Component G7122 G7122A
Gpfu Polymerase (1 Unit/λ)
10X GPfu Reaction Buffer
45 mM MgCl2
Control DNA template
Control Primer mix
100 Unit
(2 X 0.625 ml)
(2 X 0.625 ml)
10 µl
10 µl
2X100 Unit
(4 X 0.625 ml)
(4X 0.625 ml)
10 µl
10 µl
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Description
DeltaQ polymerase is a truncated version of Taq DNA
Polymerase, lacking the first 278 amino acids. To enhance its
DNA binding affinity a dsDNA binding domain protein has been
fused with this truncated polymerase. Normally DeltaQ
polymerase is able to amplify upto 2 kb from lambda DNA. Its
enhanced binding property endow It to amplify from very low
amount template (~ fmole level). DeltaQ polymerase also
contains mutations in its polymerase domain that make it
resistant to inhibitors present in body fluid. This resistant nature
to pcr inhibitors, makes DeltaQ polymerase a suitable choice for
PCR from whole blood , serum, urine. DeltaQ polymerase
tolerates up to 15% whole blood and 50-60% blood serum in a
50 ml reaction which make it unique in its character from rest of
the available polymerases. Apart from this special characteristics
DeltaQ polymerase can amplify from high GC rich template.
Applications
l PCR for diagonistic samples
l PCR for forensic sample
l PCR for GC rich amplicon
l RT-PCR
l qPCR
Unit Definition
One unit of Delta Q Taq DNA Polymerase is the amount of
enzyme required to incorporate 10 nmoles of
deoxyribonucleotide into DNA in 30 min at 74°C.
Storage
-20 ° C to -10 ° C in a non-frost-free freezer. Guaranteed
stable for 2 years when stored properly. Avoid repeated
freeze / thawing of reagents.
Kit Components
Features
æ Easy to use for PCR upto 2 kb
æ Designed to be compatible with existing assay systems
æ Amplify from very low amount of template
æ Resistant to PCR Inhibitors, can amplify in
æ presence of blood, serum and urine
æ Generates PCR products with 3'-dA overhangs
Characteristics of Delta Q: A. DeltaQ can amplify upto 2 kb on lambda DNA template.
B. DeltaQ can amplify on minimum amount of template (from femtogram amount). 1 kb
PCR on lambda DNA from indicated amount of template given template.
C. Extension speed of taq polymerase. DeltaQ can amplify 2 kb size fragment at a minimum
of 30 sec/ kb speed during extension.
D. Amplification on GC rich template: DeltaQ can amplify on strong template like high GC
content in presence of special buffer (up to 70%).
E. PCR amplification in presence of whole blood:
DeltaQ can tolerate up to 15% whole blood in PCR mix (7.5 µlit in 50 µlit reaction),
allowing a wide range of blood usage during direct PCR from blood (as template).
F. A comparative of amplification efficiency of DeltaQ, G9 taq and Vent polymerase: 1 kb
lambda based PCR was done in presence of 10%, 12% whole blood with the three
polymerases in their respective buffers.
Delta Q Polymerase
100 60 20 2
1 k
b la
dder
5% 8% 10% 12% 15%
1 k
b l
ad
de
r
1 kb
HighDeltataQ G9 taq polymerase
WHOLEBLOOD 10% 10% 10% 12%12%12%
Vent
GC Rich Template
70%65%
200bp
1 k
b lad
der
1 k
b lad
der
5 s
ec/k
b
10 s
ec/k
b
15 s
ec/k
b
30 s
ec/k
b
2 kb
A
C D
E
F
WHOLEBLOOD
Component G7122 G7122A
DeltaQ Polymerase (1unit/λ)
10X DeltaQ Reaction Buffer
30 mM MgCl2
Control DNA template
Control Primer mix
250 Unit
1 X 1.25 ml
1 X 1.25 ml
10 µl
10 µl
4 X 250 Unit
4 X 1.25 ml
4 X 1.25 ml
10 µl
10 µl
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Description
T4 DNA Ligase catalyzes the joining by the formation of
phosphodiester bonds between 3'-OH termini and 5'-P termini
of sticky or blunt ended DNA strands. The molecular weight of
T4 Ligase is ~62,000 Da. For ligation, the optimal reaction pH is 2+7.6, and T4 ligase requires Mg and ATP as cofactors.
Applications
l Cloning of restriction enzyme generated DNA
fragments.
l Cloning of PCR products.
l Joining of double-stranded oligonucleotide linkers or
adaptors to DNA.
Unit Definition
One unit is defined as the amount of enzyme required to give
50% ligation of HindIII fragments of λ DNA (5´ DNA termini
concentration of 0.12 µM, 300- µg/ml) in a total reaction
volume of 20 ml in 30 minutes at 16°C in 1X T4 DNA Ligase
Reaction Buffer.
Storage
-20 ° C to -10 ° C in a non-frost-free freezer. Guaranteed
stable for 2 years when stored properly. Avoid repeated
freeze / thawing of reagents.
Kit Components
Featurel Nick repair in duplex DNA.
l Self-circularization of linear DNA.
l Fully Ligated with in 30 minit at Room Temperature
Source
æ E.coli cells with a cloned gene 30 from bacteriophage T4.
Inhibition and Inactivation
æ T4 DNA Ligase is strongly inhibited by NaCl or KCl at
concentrations higher than 200 mM.
æ Inactivated by heating at 65°C for 10 min or at 70°C for 5
min.
T4 DNA Ligase
Component GTDL01 GTDL02 GTDL03
T4 DNA Ligase(5U/ul)
10X T4 DNA Ligase
Buffer
100 Unit
150�ml
250 Unit
375�ml
1000 Unit
1500�ml
l D
NA
Dig
est
ed
Usi
ng
Hin
dII
I
Lig
ate
d w
ith
T4
DN
A L
igase
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Description
Codon optimized Taq DNA Polymerase gene of Thermus
aquaticus was cloned and purified from E. coli host. The enzyme
consists of a single polypeptide with a molecular weight of
approximately 94 kDa. Ready Load PCR Master Mix is a ready-
to-load master mix. It is a convenient way of amplifying DNA
fragments without the need to thaw individual components,
reducing the risk of contamination and pipetting errors.
Presence of a tracking dye helps to load and track migration of
DNA on agarose gel. The G9 Taq DNA Polymerase, dNTPs,
reaction buffer and magnesium chloride are all present in the
mix. Tracking dye is present in such an amount that its migration
should be visible but should not mask DNA to observe. Presence
of precipitant and tracking dye eliminates any chances of
pipetting error prior gel load thus making this 2X G9 mix as an
appropriate choice for semiquantitative PCR analysis.
Applications
l Ready Load PCR Master Mix is a ready-to-load master
mix
l Tracking dye is present in such an amount that its
migration should be visible but should not mask DNA
to observe
Unit Definition
One unit incorporates 10nmol of deoxy-ribonucleotide into
acid-insoluble product in 30 minutes at 74°C. Unit assay
conditions: 25 mM TAPS (pH 9.3), 50 mM KCl, 2 mM MgCl2, 1
mM DTT, 0.2 mM dATP, dCTP, dGTP, dTTP utilizing
M13mp18DNA as template.
Storage
-20°C to -10°C in a non-frost-free freezer. Guaranteed stable
for 2 years when stored properly.Avoid repeated
freeze/thawing of reagents
Kit Components
Features
æ Reducing the risk of contamination and pipetting
errors
æ Designed for easy and regular use
æ Half-life is more than 40 min at 95°C
æ Generates PCR products with 3'-dA overhangs
æ It can amplify up to 6 kb from lambda DNA.
Fig.1.
PCR amplification with 2X G9 Taq Readyload PCR Master Mix from
lambda DNA 1st 2nd and 3rd lane : 6 kb PCR amplification reactions,
using 2X G9 Taq Readyload PCR Master Mix in 50 ml final reaction
volume. 5 ml loaded for analysis in 1% agarose gel. 4th Lane: molecular
size marker - 1 kb DNA Ladder.
2X G9 Taq Readyload PCR Master Mix
Component G7124 G7124A
2X PCR Master Mix
Control DNA template
Control primer Mix
(1000 ml X 2)
10�ml
10�ml
(1000 ml X 20)
10�ml
10�ml
1 kb
lad
der
6 kb Amplification of
l DNA using Ready to
load Master Mix
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PolymeraseStaini
ng Reagents
5′–>3′
Exonuclease
3′–>5′
Exonucleaselimit
Thermal
StabilityRequire
Extension
Rate
Extension
from NickApplications
G9 Taq
polymerase &
Master Mix
+ - + 1 kb/ min + Routine PCR up to 6 kb
Hi-G9 Taq
polymerase &
Master Mix
+ - + 1 kb/ min +
Routine PCR upto 8 kb, with
high productivity, template
with High GC content.
Prime Taq
polymerase &
Master Mix
+ - ++ 1 kb/ min + Long PCR up to 10 kb.
Hi prime Taq
polymerase &
Master Mix
+-
++ 1 kb/ min +
Long PCR more than 12 kb
(with enhanced
productivity)
Gpfu DNA
polymerase- + + 0.5 kb/ min -
High-fidelity PCR, (such as
gene cloning, gene
expression or mutation
analysis), upto 4 Kb
DeltaQ
polymerase- - - 2 kb/ min +
Low template requirement,
Direct PCR from body fluid,
RT PCR
upto 2 Kb.
Molecular Biology
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Plant Leaf / Blood / Tissue
Prescribed
Sample
amount
Incubate in fastract Buffer>O95 C for 15 min
Use as Template for PCR reaction
Collect
Fig. 1.: Streamline Protocol for FasTract Direct PCR Kit
DNA isolation is a time consuming, laborious procedure
specially when working with a huge number of samples.
Moreover sometimes purified DNA contains carry-over
inhibitors from crude extraction methods. FasTract Direct PCR
Kit provides ease for direct PCR from a wide variety of samples
without isolation of total genomic DNA. FasTract Direct PCR Kits
are designed to perform PCR directly from crude samples like
plant leaves, animal tissues, blood, saliva, cultured cells and even
samples like scalp and body hair without prior DNA isolation
and purification. Chaotropic salts and detergents present in
FasTract Lysis Buffer ensure better and efficient lysis even with
tough samples like animal tissues and hair. FasTract PCR Mix
contains a genetically modified Hi Fidelity DNA polymerase
which can amplify even in presence of a large number of PCR
inhibitors. The kit ensures high yield in PCR amplification and is
time saving as DNA isolation from samples can be avoided prior
to PCR. The kit is recommended for end-point PCR. Samples are
needed to collect as the prescribed amount, incubated at 950C
in presence of FasTract Lysis buffer for 15 min only. This lysate is
to be used for PCR reaction as template. The lysate is only
compatible with the FasTract PCR master mix.
When working with plant samples, like plant leaves storage of
samples often becomes a matter of concern. FasTract Lysis
Buffer that comes along with FasTract Plant Direct PCR Kit is an
ideal storage buffer for leaf samples that will be further used for
direct amplification. Leaf samples can be stored in FasTract lysis 0buffer at room temperature for 5 days, at 4 C upto 4 weeks and
0indefinite time at -20 C .
Body fluids like saliva, clotted blood, buccal and pap swabs and
even tough samples like skin and hair are suitable samples for
FasTract Direct PCR Kit. Thus the kit finds huge application in
diagnosis of clinical samples and even for forensic detection.
Introduction
Fastract Direct PCR Kit
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Introduction
Plant DNA isolation is a time consuming, laborious procedure
specially when working with a huge number of samples. FasTract
Plant Direct PCR Kit is designed to perform PCR directly from
plant leaves without prior DNA isolation and purification. Fresh
plants, plant material stored at 4°C or frozen are all suitable
templates for this kit. Plant leaves could also be collected
directly in the FasTract plant Lysis Buffer and stored at room 0temperature for at least 48hr, at 4oC for 5 days and at -20 C for
at least one months. Caotrophs and detergents present in
FasTract Lysis Buffer ensure better and efficient lysis even with
tough samples like palm and coconut. FasTract plant PCR Mix
contains a genetically modified Hi Fidelity DNA polymerase
which can amplify even in presence of PCR inhibitors in plant
lysate. The kit ensures high yield in PCR amplification and is time
saving as DNA isolation from samples can be avoided prior to
PCR. The control primers provided can amplify a highly
conserved region from plant DNA. Purified plant gDNA is also
provided as a positive control for the PCR reactions. The kit is
recommended for end-point PCR.
Guidelines of Sample Preparation
l Single hole paper puncher must be used to obtain
small and uniform leaf discs (2mm diameter)
l It is very important to clean the cutting edge every
time before sampling to prevent cross-contamination
between samples
l 2 % sodium hypochlorite solution or 70% Ethanol
should be used for cleaning.
l Punch out a disc from the plant leaf using the
sampling tool and place disc directly into the PCR
tubes.
l Add 50ml Fas ract Lysis Buffer into each tubes,
l vortex well and incubate them at 950C for 15mins.
l Use 2. 5ml of this lysate as template for the PCR
reaction
l The FasTract Lysis buffer can be used for collection
and storage of leaf disc samples.
Guidelines for Control reaction
We recommend setting up a control PCR reactions with both the
purified DNA (provided) as well as the FasTract leaf disc lysate
used in the actual experiment to ensure that the PCR conditions
are optimal. If the positive control with purified DNA fails, the
PCR conditions should be optimized before continuing further.
It is recommended to add a no-template control to all PCR
assays.
Fig.1.
Different plant leaves after incubation with FasTract lysis
buffer.
Fig.2.
Endpoint PCR of 35 cycles was done using plant specific RBCL primer pair
Oon various plant leaves Leaf discs were incubated at 95 C for 15 mins 50
ml of Fastract modified buffer. 2.5 ml of this is used as template.
Gra
m
Orc
hid
Maiz
e
Pa
lm
Man
go
Ch
illi
Co
locasi
a
Co
co
nu
t
Pa
paya
Un
kn
ow
+ve c
on
100
bp
L
Fastract Direct PCR Kit for Plant
Key Components Amount
Fastract Lysis Buffer
Fastract 2X PCR Mix (Fastract DNA Polymerase, Reaction buffer, dNTP(10mM))
Control Primer Mix (10mM each)
50 ml
22 ml
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Guidelines For PCR setup
l Take a single leaf disc in a PCR tube and add 50µl of
FasTract Lysis Buffer in it.
l For samples collected directly in FasTract Lysis buffer,
next step is not required for soft plant leaves. S t i l l ,
heating the samples will ensure better lysis.
l Vortex the sample vigorously and incubate the FasTract oLysis Buffer added sample at 95 C for 15 min in a PCR
machine lead heating condition.
l Add 22ml 1X Fastract PCR mix in a Flat Capped PCR
tube.
l Add 0.25 ml of each Primers (from 10mM stock) to the
master mix.
l Use 2. 5ml of FasTract lysate as template for the PCR
reaction.
l Set up PCR as below.
Troubleshooting
l For tough and dry leaf samples increase the oincubation time at 95 C
l If PCR fails due to high amount of PCR inhibitors in
the leaf disc lysate, then the amount of lysis buffer can
be increased to 75-100ml
l If PCR fails even after increasing the volume of lysis
buffer, incubate half of the leaf disc in lysis buffer
instead of the entire disc.
Storage
FasTract Direct PCR Kit® is shipped on gel ice. Upon arrival,
store the components at +4 ° C. Do not freeze.
Ordering Information
Important Notes
æ Carefully mix and spin down all tubes before opening to
ensure homogeneity and improve recovery. The PCR setup
can be performed at room temperature.
æ Always add the plant sample last to the reaction.
æ It is recommended to eject the leaf disc into the empty tube
and add the lysis buffer to ensure that the entire disc is
dipped into buffer. Make sure that you see the sample disc in
the solution.
æ We recommend using fresh plant material for best results,
oeven though plant material stored at +4 ° C or -20 C can also
be used.
æ For extension, use 1min for 1Kb amplicon size.
æ The FasTract Lysate must be added such that it is 10% of the
total reaction volume i.e if the total reaction volume is 25ml
then 2.5ml of leaf disc lysate must be added to the reaction.
Cat. # Product Pack Size
G45311AFasTract Direct PCR
Kit® for Plant100 rxn
G45311BFasTract Direct PCR
Kit® for Plant500 rxn
95 ° C 95 ° C72 ° C 72 ° C
40x
10 s
t min 5 min
hold
5 min 10 sTm
t min= extension time: 1 min /kb
PCR Cycle
4 ° C
Fastract Direct PCR Kit for Plant
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Fastract Direct PCR Kit for Blood
IntroductionFasTract Blood Direct PCR Kit is designed to perform PCR
directly from blood, and saliva without prior DNA purification.
Freshly collected blood, blood stored with anticoagulants,
clotted blood and even blood spots on paper discs, all are
suitable samples for this FasTract Blood Direct PCR kit. The kit
employs a specially engineered high fidelity DNA Polymerase
enzyme that exhibits high resistance to many PCR inhibitors
found in blood and other bio-fluids. The kit ensures high yield
and is time saving as DNA isolation from samples can be avoided
prior to PCR. The kit is recommended for end-point PCR.
Fig.1.
CR reaction on blood, collected from different animals, using
species specific GAPDH primers. 20ml of blood samples were
lysed using 50ml of FasTract Blood Lysis Buffer and PCR reaction
was set up using FasTract 1X mastermix for blood
Guidelines of Sample Preparationl Blood stored with anticoagulants, clotted blood and
even blood spots on paper discs, all are suitable samples
for this Fastract Blood Direct PCR kit.
l When working with clotted blood or blood, 10-20 ml
blood is recommended
l In case of spotted blood on paper discs use a single hole
paper puncher to punch out a spotted blood disc. This
will be the sample.
l Collect the blood samples in a PCR tube , add 100 ml
Fastract Blood Lysis Buffer into each tubes, vortex well 0and incubate them at 95 C for 15 mins.
l After incubation centrifuge the PCR tubes at 15000 rpm
for 2 mins. Collect the clear supernatant in a separate
microfuge tube
l Use 1.5 ml of this clear lysate as template for the PCR
reaction.
Guidelines of PCR Setupl Add 47.5 ml 1X Fastract Blood Direct PCR mix in a Flat
Capped PCR tube.
l Add 0.5 ml of each Primers (from 10mM stock) to the master
mix.
l Use1. 5 ml of Fastract Blood lysate as template for the PCR
reaction.
l While setting up the PCR cycle keep the initial denaturation 0time to be 5 mins at 950C followed by denaturation at 95 C
for 10 -15s and annealing at the desired Tm. For extension,
use 1min for 1Kb amplicon size.
Troubleshootingl In case of no PCR reaction dilute the lysate In case of non-
specific amplification, increase the annealing temperature,
reduce the total number of cycles or design a new set of
primers.
Shipping and storagel Fastract Tissue Direct PCR Kit is shipped on gel ice. Upon
arrival, store the components at +4 ° C. Do not freeze.
Important Notesl Carefully mix and spin down all tubes before opening to
ensure homogeneity and improve recovery. The PCR setup
can be performed at room temperature.
l Always add the Blood lysate last to the reaction.
l When working with clotted blood vortex vigorously after
addition of lysis buffer
l Always ensure that the volume of lysate that is being added
to the PCR reaction is 3% of the total reaction volume. Do
not add more lysate to the reaction mix.
Key Components
FasTract Blood Buffer
FasTract 2X PCR Mix (Fastract DNA Polymerase, Reaction buffer, dNTP(10mM))
Control Primer Mix (10mM each)
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DescriptionFastract Direct PCR Kit™ for Blood is a rapid PCR mastermix that
eliminates the requirement of genomic DNA isolation prior PCR
reaction. This improved master mix is a ready load 2X Master
Mix, allowing researcher to load the PCR product directly in the
gel. Addition of loading dye is not required. FasTract direct PCR
kit comes with three different components.
1. RBC Lysis Buffer
2. Pellet dissolving buffer
3. 2X mastermix
This is recommended to lyse the RBC and harvest the WBC
(protocol provided with the kit) which to be resuspended and
lysed in Pellet dissolving buffer. This lysate would be used as
template for the PCR reaction. Fastract amplification after
removal of RBC is always recommended because it helps to have
efficient amplification for long amplicons and also for multiplex
amplification (Figure 1 and Figure 2). Direct lysis of whole blood
followed by amplification with Fastract PCR master mix shows
efficient PCR reaction for short amplicons (less than 500bp) but
the same for long amplicons (more than 1 kb) are compromised
(Figure 3 and 4 respectively). Amplification efficiency from blood
with nucleated RBC's are also checked and efficient
amplification were observed (Figure 5). The pellet dissolving
buffer has been formulated in such a way, lysate would be
compatible to amplify with any market leading Taq DNA
Polymerase (Figure 6).
Fig.1.
Amplification from direct blood sample Blood samples were
processed to remove RBCs and lysate were prepared from WBCs
only. 40 cycle endpoint PCR directly from lysate to amplify a
small amplicon from b-actin gene (<1kb) and a large amplicon
from G6PD gene (>1kb) with Fastract Blood Direct PCR Kit.
Fig.2.
Multiplex PCR using FasTract Direct PCR Kit 40 cycle multiplex
PCR directly from blood lysate to amplify GAPDH and H2B gene
specific region with Fastract Blood Direct PCR Kit.
Fig.3.
Equal amplification efficiency with or without vigorous mixing
Different volume of xeroPolymerase were reconstituted with or
without vortexing after addition of water, primers and
templates. Equal amplification found in both the cases.
Fastract Direct PCR Kit for Blood
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Fastract Direct PCR Kit for Blood
Fig. 4.
Amplification efficiency for short sized amplicon 40 cycle
endpoint PCR with Fastract Blood Direct PCR kit. PCR is done
directly from different volumes of human blood lysed in 30 ml
FasTract Blood Pellet Dissolving Buffer. Different volume of
blood lysate is used as template to amplify housekeeping gene
b-actin from human genome. WBC lysate is used as appositive
control for the reaction.
Fig.5.
FasTract Direct PCR amplification on blood with nucleated RBC
40 cycle endpoint PCR with Fastract Blood Direct PCR Kit from
species with nucleated RBC's like Fish and Chicken. 3ml of blood
is lysed in different volumes of Fastract Blood Lysis Buffer and
different volume of lysate is used as template for PCR to amplify
a housekeeping gene from the individual species. Genomic DNA
is used as a positive control for PCR amplification.
Fig.6.
Compatibility of Fastract Lysis Buffer with other Taq DNA
Polymerase 40 cycle endpoint PCR with human blood lysate to
amplify housekeeping gene b-actin from human genome. Blood
lysate is prepared with Fastract Blood Lysis buffer and the lysate
is used as a template for PCR with G9.
Ordering Information
Cat. # Product Pack Size
G45312A FasTract Direct PCR Kit® for Blood 100 rxn
G45312B FasTract Direct PCR Kit® for Blood 500 rxn
G45312 FasTract Direct PCR Kit® for Blood 1000 rxn
G45312QFasTract Blood lysis Buffer for
Blood (Without Mastermix)1000 rxn
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DescriptionFasTract Cultured cell Direct PCR Kit is designed to perform PCR
directly from cultured cell without prior DNA purification. Fresh
or frozen cells all are suitable templates for this kit. The kit
employs a specially engineered high fidelity DNA Polymerase
enzyme that exhibits high resistance to many PCR inhibitors
found in cell or tissue culture media. The kit ensures high yield
and is time saving as DNA isolation from samples can be avoided
prior to PCR.The kit is recommended for end-point PCR.
Fig.1.
PCR on cell lines using specific GAPDH Primers for CHO and b-
actin for the human cell lines. 0.2 million cells were lysed using
50ml of Fastract lysis buffer and PCR reaction was set up using
Fastract 1X Master Mix for Cultured cells
Guidelines of Sample Preparationl When working with cultured cell, use maximum 1 million or
as least as 10000 cells.
l The cells must be pellet down and must be washed with 1X
PBS 2-3 times.
l The cells must be suspended in 1X PBS solution
l Collect 25 ml of this cell suspension in a PCR tube, add 100ml
FasTract Cultured cell Lysis Buffer into each tubes, vortex owell and incubate them at 95 C for 15 mins.
l After incubation centrifuge the PCR tubes at 15000rpm for 2
mins. Collect the clear supernatant in a separate micro-fuge
tube
l Use 1.5 ml of this clear lysate as template for the PCR
reaction.
Troubleshootingl In case of no PCR reaction dilute the cell lysate 1:10 or
1:50 with FasTract cultured cell lysisbuffer and use 1.5 ml
of this dilution as template.
l In case of non-specific amplification, increase the
annealing temperature, reduce the total number of cycles
or design a new set of primers.
l
Shipping and storagel Fastract Tissue Direct PCR Kit is shipped on gel ice. Upon
arrival, store the components at +4°C. Do not freeze.
l
Important Notesü Carefully mix and spin down all tubes before opening to
ensure homogeneity and improve recovery. The PCR setup
can be performed at room temperature.
ü Always add the Blood lysate last to the reaction.
ü Always ensure that the volume of lysate that is being added
to the PCR reaction is 3% of the total reaction volume. Do
not add more lysate to the reaction mix
Important Notes
Key Components
FasTract Cell Lysis Buffer
FasTract Tissue 1X PCR Mix (FasTract DNA Polymerase, Reaction buffer, dNTP (10mM))
Control Primer Mix (10mM each)
Cat. # Product Pack Size
G45313AFasTract Direct PCR Kit® for
Cultured Cell100 rxn
G45313FasTract Direct PCR Kit® for
Cultured Cell500 rxn
Fastract Direct PCR Kit for Cultured Cell
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
GCC Biotech (India) Pvt. Ltd. | tech.support@gccbiotech.co.in | 91-33-24951044/24950004 | gccbiotech.co.in 88
DescriptionFastract Tissue Direct PCR Kit is designed to perform PCR directly
from non-fixed animal derived tissue samples tissue samples
without prior DNA purification. Fresh tissues or tissue samples 0frozen at -20 C are all suitable templates for this kit. The kit
employs a specially engineered high fidelity DNA Polymerase
enzyme that exhibits high resistance to many PCR inhibitors
found in animal tissues. The kit ensures high yield and is time
saving as DNA isolation from samples can be avoided prior to
PCR. The kit includes a pair of universal control primers that is
compatible with a number of vertebrate species .The kit is
recommended for end-point PCR.
Fig.1.
PCR on tissue samples collected from different animals using
species specific GAPDH Primers.10mg tissue samples were lysed
using 50 ml of Fastract lysis uffer and PCR reaction was set up
using Fastract 1X mastermix for Tissue
Fig.2.
Fastract Lysis and PCR on different tissue of mice.10mg of tissue
samples were lysed in Fastract Lysis Buffer and PCR reaction
were done using FasTract 1X Master Mix.
Guidelines of Sample Preparationl When working with tissue samples, use of 2mg of tissue is
recommended for direct PCR
l Use a scalpel to cut out the tissue.
l The scalpel must be thoroughly cleaned with 2% Sodium
hypochlor ide or 70% Ethanol to prevent cross
contamination.
l Collect the tissue samples in a PCR tube, add 100 ml Fastract
Tissue Lysis Buffer into each tubes, vortex well and incubate
them at 950C for 15 mins.
l After incubation centrifuge the PCR tubes at 15000 rpm for 2
mins. Collect the clear supernatant in a separate microfuge
tube.
l Use 1.5 ml of this clear lysate as template for the PCR
reaction.
Guidelines for PCR Setupl Add 47.5ml 1X Fastract Tissueu cell Direct PCR mix in a Flat
Capped PCR tube
l Add 0.5ml of each Primers (from 10mM stock) to the master
mix
l Use1.5ml of Fastract cultured cell lysate as template for the
PCR reaction
l While setting up the PCR cycle keep the initial denaturation
time to be 5 mins at 950C followed by denaturation at 950C
for 10 -15s and annealing at the desired Tm. For extension,
use 1min for 1Kb amplicon size.
Troubleshootingl In case of no PCR reaction dilute the tissue lysate 1:10 or
1:50 with Fastract Tissue lysis buffer and use 1.5ml of this
dilution as template
l In case of non-specific amplification, increase the
annealing temperature, reduce the total number o f
cycles or design a new set of primers.
Important Notesü Carefully mix and spin down all tubes before opening to
ensure homogeneity and improve recovery. The PCR
setup can be performed at room temperature.
ü Always add the Tissue sample lysate last to the reaction.
ü It is recommended to add the tissue samples into the empty
tube and add the lysis buffer to ensure that the entire
sample is dipped into buffer. Make sure that you see the
sample in the solution.
ü Always ensure that the volume of lysate that is being added
to the PCR reaction is 3% of the total reaction volume. Do
not add more lysate to the reaction mix
ü Always ensure that the volume of lysate that is being added
to the PCR reaction is 3% of the total reaction volume. Do
not add more lysate to the reaction mix.
Fastract Direct PCR Kit for Tissue
Key Components
Fastract Tissue Lysis Buffer
Fastract Tissue 1X PCR Mix (FasTract DNA Polymerase, Reaction buffer, dNTP (10mM))
Control Primer Mix (10mM each)
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GCC Biotech (India) Pvt. Ltd. | tech.support@gccbiotech.co.in | 91-33-24951044/24950004 | gccbiotech.co.in
DescriptionFasTract Tissue Direct PCR Kit is designed to perform PCR
directly from hair blood, and saliva without prior DNA
purification. Freshly collected blood, blood stored with
anticoagulants, clotted blood and even blood spots on paper
discs, all are suitable samples for this FasTract Blood Direct PCR
kit. The kit employs a specially engineered high fidelity DNA
Polymerase enzyme that exhibits high resistance to many PCR
inhibitors found in blood and other biofluids. The kit ensures
high yield and is time saving as DNA isolation from samples can
be avoided prior to PCR. The kit is recommended for end-point
PCR.
Fig.1.
FasTract Lysis and PCR using different tissues of mice. 10 mg
of tissue samples were lysed in FasTract Lysis Buffer and PCR
reaction were done using FasTract 1X Master Mix
Guidelines of Sample Preparationl Collect the samples in a PCR tube, add 100 ul Fastract
Forensic Lysis Buffer into each tube, vortex well and incubate othem at 95 C for 15 mins.
l After incubation, centrifuge the PCR tubes at 15000 rpm for
2 mins. Collect the clear supernatant in a separate micro-
fuge tube
l Use 1.5 ul of this clear lysate as template for the PCR
reaction.
Guidelines for PCR Setupl Add 47.5 ml 1X Fastract Tissue cell Direct PCR mix in a flat
capped PCR tube
l Add 0.5 ml of each primers (from 10 mM stock) to the master
mix.
l Use1. 5 ml of Fastract cultured cell lysate as template for the
PCR reaction.
l While setting up the PCR cycle keep the initial denaturation o 0time to be 5 mins at 95 C followed by denaturation at 95 C
for 10 -15s and annealing at the desired Tm. For extension,
use 1min for 1 Kb amplicon size.
Troubleshootingl In case of no PCR reaction dilute the tissue lysate 1:10 or
1:50 with Fastract Tissue lysis buffer and use 1.5 ml of this
dilution as template
l In case of non-specific amplification, increase the
annealing temperature, reduce the total number o f
cycles or design a new set of primers.
Important Notesü Carefully mix and spin down all tubes before opening to
ensure homogeneity and improve recovery. The PCR setup
can be performed at room temperature.
ü Always add the Tissue sample lysate last to the reaction.
ü Always ensure that the volume of lysate that is being added
to the PCR reaction is 3% of the total reaction volume. Do
not add more lysate to the reaction mix.
Ordering Information
Fastract Direct PCR Kit for Forensic Samples
Key Components
Fastract Tissue Lysis Buffer
Fastract Tissue 1X PCR Mix (Fastract DNA Polymerase, Reaction buffer, dNTP (10 mM)) Control Primer Mix (10 mM each)
Cat. # Product Pack Size
G45316AFastract Direct PCR Kit® for
Forensic Samples100 rxn
G45316Fastract Direct PCR Kit® for
Forensic Samples500 rxn
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DescriptionMolecular markers are now widely used to track loci and
genome regions in several crop-breeding programmes. PCR-
based markers analysis and gene expression studies are
increasing exponentially. In these studies high-quality and intact
DNA is the keystone for performing PCR based research.
However, individual species of plants can behave differently
during extraction of due to difference in metabolic activities.
Rice (Oryza sativa L.) synthesize a wide spectrum of
polysaccharides and polyphenols including flavonoids and
other secondary metabolites which interfere with the extraction
of pure genomic DNA. These polysaccharides co-precipitate
during DNA extraction and also known to inhibit polymerase
activity
Fastract Rice Direct PCR Kit provides ease for direct PCR from
Rice leaves without isolation of total genomic DNA. It is an
improved version of GCC Biotech Fastract Plant Direct PCR Kit.
This newly improved and optimized version of Fastract Direct
PCR Kit has been validated over different varieties of rice and
analyzed using 50 SSR genetic markers, distributed across 12
rice chromosomes reported in GRAMENE website
http://archive.gramene.org/species/oryza/rice_intro.html
Simple sequence repeat (SSR) is one of the most robust marker
for identifying rice varieties for assessment of genetic diversity
and population structure.
OhighlightDirect PCR—sample is added directly to PCR reactions therefore
there is no need for time-consuming and expensive DNA
purification steps.
Storage protocol available—allows multiple PCR reactions from
one tiny sample and allows re-testing
Spec i a l l y eng inee red H i F ide l i t y Fa s t r a c t™ DNA
polymerase—extremely short PCR protocol times
·Master Mix format with premixed gel loading dye—minimizes
possibility of cross-contamination, reduces sample handling
and allows direct loading on gel
Marker
Name
Chrom
osome
No
Volume
of Lysis
Buffer
(ml)
Incubati
on Time
PCR
Cycles
Optimi
zed Tm
(⁰C)
Amplicon
size (base
pairs)
Type of
Amplification
Rm 495
Rm 1
Rm 283
Rm 259
Rm 312
Rm 5
Rm 237
Rm 431
Rm 154
Rm 452
Rm 489
OSR 13
Rm 338
Rm 55
Rm 514
Rm 307
Rm 124
Rm 507
Rm 413
Rm 161
Rm 178
Rm 334
Rm 133
Rm 510
Rm 454
Rm 162
Rm 125
Rm 11
Rm 455
Rm 118
Rm 408
Rm 152
Rm 25
Rm 44
Rm 284
Rm 433
Rm 447
Rm 316
Rm 105
Rm 215
Rm 474
Rm 271
Rm 171
Rm 484
Rm 552
Rm 536
Rm 287
Rm 144
Rm 19
Rm 277
1
1
1
1
1
1
1
1
2
2
3
3
3
3
3
4
4
5
5
5
5
5
6
6
6
6
7
7
7
7
8
8
8
8
8
8
8
9
9
9
10
10
10
10
11
11
11
11
12
12
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
15 Mins
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
54
52
58
54
50
54
58
58
66
52
52
58
60
54
56
54
64
56
54
62
62
58
64
58
58
65
65
56
52
62
58
52
56
48
60
58
60
56
65
58
56
50
58
58
56
56
50
64
52
54
159
78
156
172
>500
113
133
251
183
209
271
99
184
>500
245
129
271
258
79
>500
117
>500
230
122
>500
229
>500
126
131
159
129
152
141
>500
148
312
111
197
134
150
252
>500
329
299
195
243
107
225
>500
117
Intense
Moderate
Intense
Intense
Moderate
Moderate
Intense
Intense
Intense
Intense
Intense
Moderate
Moderate
Intense
Intense
Faint
Intense
Intense
Moderate
Faint
Intense
Faint
Intense
Intense
Moderate
Intense
Moderate
Intense
Intense
Intense
Intense
Intense
Faint
Faint
Intense
Intense
Intense
Intense
Moderate
Intense
Faint
Moderate
Intense
Moderate
Faint
Intense
Faint
Intense
Moderate
Intense
Fastract Direct PCR Kit for Rice
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GCC Biotech (India) Pvt. Ltd. | tech.support@gccbiotech.co.in | 91-33-24951044/24950004 | gccbiotech.co.in
DescriptionqPCR is the technique used as a standard now a days for assay of
gene expression, copy number determination, disease diagnosis
and several other application. GCC Biotech has developed qPCR
mastermix using all the endogenous reagents and formulations,
starting from the thermostable polymerase and its buffer,
fluorescent dye or the passive reference dye.
Detection Capability as low as 1 copy
of target 10 fold serial dilution of human genomic DNA was used as
template for amplification with b-actin specific primer.
Sensitivity was detected up to 1 copy (2.5pg) of template
(amplification plot). Amplification on serial dilution of template
shows linearity of amplification on wide range of target quantity
(Standard curve). Melting curve shows no nonspecific
amplification.
Fig.1.
Amplification of different target Same amount of A.DNA was
used to amplify different targer regions. Amplification plot
shows different amplification efficiency of the target regions.
Melt curve shows different amplified products.
Detect Wide Range of Target from
Same TemplateAmplification of different target: Same amount of genomic
DNA was used to amplify different target regions. Amplification
plot shows different amplification efficiency of the target
regions. Melt curve shows different amplified products.
Fig.2.
Detection capability as low as 1copy of target Detection
capability as low as 1copy of target: 10 fold serial dilution of
human genomic DNAwas used as template for amplification
with (3-actin specific primer. Sensitivity was detected upto
1copy (2.5pg) of template (amplification plot). Amplification
on serial dilution of template shows linearity of amplification
on wide range of target (Standard curve). Melting curve shows
no non specific amplification.
Featureæ Exceptional sensitivity and specificity: powered by deltaQ,
a cold sensitive PCR enzyme that gives exceptional natural
hot-start activity, and optimized buffer formulation to
eliminate the possibility of primer-dimer formation and/or
non-specific amplification up to a stringent level.
æ Out performs any big brand in this product range:
Provides at least 10 fold more lower limit of detection
while comparing with Thermo or Applied Biosystems
qPCR master mixes.
æ Reproducible and repeatable: The C value over a broad
T dynamic range consistently provides reproducible data.
æ Superior master-mix: Produces excellent linear data with 2accur ate regression value R ~ 0.99), slope and PCR
efficiency (close to 100%) with best compatible
templateprimer
æ Compatible with broad range of Real-Time PCR
instruments: Works with any real-time PCR instrument.
æ Choose from two formulations: contains the ROX
reference dye in separate vial for your optimization to
choose the master mix with or without ROX.
Shipping and StorageqPCR master mix is shipped on dry ice. Upon arrival, store
the componentsat -20°C.
Amplification of same target on
different templategDNA was isolated from 200ml blood of 8 individuals. Equal
amount of DNA (1ng) was used as template for each
amplification. Variation on amplification plot shows variability
of genome and single peak on Melt curve shows single
amplification irrespective of the DNA source.
qPCR Master Mix
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
GCC Biotech (India) Pvt. Ltd. | tech.support@gccbiotech.co.in | 91-33-24951044/24950004 | gccbiotech.co.in 92
Fig.3.
Amplification of same target on different template gDNA was
isolated from 200�ml blood of 8 individuals. Equal amount of
DNA (1ng) was used as template for each amplification.
Variation on amplification plot shows variability of genome
and single peak on Melt curve shows single amplification
irrespective of the DNA source.
Ordering Information
Get 10-fold higher sensitivity than
the leading master mix
Fig.4.
Comparative analysis with other vendors 400 fg of DNA was
used to amplify a 300 bp fragment using 2X qPCR mix(GCR-51)
and 2x H-eff qPCR mix(GCR-61). Comparative analysis with same
amount of target-primer combination showed higher Ct value
with Thermo 2X DyNAmo Color Flash (F-416) and I- Power SYBR
Green PCR Master Mix (4367659) (amplification plots and Ct
value bar diagram). Melt curve shows specific single product
with GCC 2x qPCR mix and GCC 2x qPCR H-eff mix.
Cat # Product Pack Size
GCR-51 qPCR Master mix, SYBR Rox 1 ml
GCR-52 qPCR Master mix, SYBR Rox 5X1 ml
GCR-53 qPCR Master mix, SYBR Rox 10X1 ml
GCR-54 qPCR Master mix, SYBR Rox 5X5 ml
qPCR Master Mix
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GCC Biotech (India) Pvt. Ltd. | tech.support@gccbiotech.co.in | 91-33-24951044/24950004 | gccbiotech.co.in
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