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Lipopolysaccharide structure impacts the entrykinetics of bacterial outer membrane vesicles intohost cellsO'Donoghue, Eloise; Sirisaengtaksin, Natalie; Browning, Douglas; Bielska, Ewa; Hadis,Mohammed; Fernandez-Trillo, Francisco; Alderwick, Luke; Jabbari, Sara; Krachler, AnneMarieDOI:10.1371/journal.ppat.1006760
Document VersionPeer reviewed version
Citation for published version (Harvard):O'Donoghue, E, Sirisaengtaksin, N, Browning, D, Bielska, E, Hadis, M, Fernandez-Trillo, F, Alderwick, L,Jabbari, S & Krachler, AM 2017, 'Lipopolysaccharide structure impacts the entry kinetics of bacterial outermembrane vesicles into host cells', PLoS pathogens, vol. 13, no. 11, pp. e1006760.https://doi.org/10.1371/journal.ppat.1006760
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Download date: 08. Jun. 2020
Lipopolysaccharide structure impacts the entry kinetics of bacterial outer 1
membrane vesicles into host cells 2
Eloise J O’Donoghue1, Natalie Sirisaengtaksin
2, Douglas F. Browning
1, Ewa Bielska
1, 3
Mohammed Hadis3, Francisco Fernandez-Trillo
3, Luke Alderwick
1, Sara Jabbari
1,4 and Anne 4
Marie Krachler4,*
5
6
1Institute of Microbiology and Infection, School of Biosciences, University of Birmingham, 7
Edgbaston, B15 2TT Birmingham, UK 8
2Department of Microbiology and Molecular Genetics, University of Texas McGovern Medical 9
School at Houston, Houston, TX, 77030, USA. 10
3Institute of Microbiology and Infection, School of Chemistry, University of Birmingham, 11
Edgbaston, B15 2TT Birmingham, UK 12
4School of Mathematics, University of Birmingham, Edgbaston, B15 2TT, Birmingham, UK 13
*Correspondence to: Anne Marie Krachler (anne.marie.krachler@uth.tmc.edu) 14
15
Keywords: outer membrane vesicles, lipopolysaccharide, serotype, FRET kinetics, extracellular 16
vesicles, cargo uptake, vesicle trafficking, endocytosis; 17
18
LPS composition impacts OMV entry kinetics | 2
AUTHOR SUMMARY 19
All Gram negative species of bacteria, including those that cause significant disease, release 20
small vesicles from their cell membrane. These vesicles deliver toxins and other virulence factors 21
to host cells during infection. Current methods for studying host cell entry are limited due to the 22
nanometer size and rapid uptake kinetics of vesicles. Here we developed a method to monitor the 23
rapid vesicle entry into host cells in real-time. This method highlighted differences in kinetics 24
and entry route of vesicles into host cells, which varied with the bacterial cell wall composition 25
and thus, the vesicle surface. Increased understanding of vesicular entry mechanisms could 26
identify targets which may allow us to combat infections by inhibiting delivery of vesicle-27
associated toxins to host cells. 28
29
LPS composition impacts OMV entry kinetics | 3
ABSTRACT 30
Outer membrane vesicles are nano-sized microvesicles shed from the outer membrane of Gram-31
negative bacteria and play important roles in immune priming and disease pathogenesis. 32
However, our current mechanistic understanding of vesicle - host cell interactions is limited by a 33
lack of methods to study the rapid kinetics of vesicle entry and cargo delivery to host cells. Here, 34
we describe a highly sensitive method to study the kinetics of vesicle entry into host cells in real-35
time using a genetically encoded, vesicle-targeted probe. We found that the route of vesicular 36
uptake, and thus entry kinetics and efficiency, are shaped by bacterial cell wall composition. The 37
presence of lipopolysaccharide O antigen enables vesicles to bypass clathrin-mediated 38
endocytosis, which enhances both their entry rate and efficiency into host cells. Collectively, our 39
findings highlight the composition of the bacterial cell wall as a major determinant of secretion-40
independent delivery of virulence factors during Gram-negative infections. 41
42
LPS composition impacts OMV entry kinetics | 4
INTRODUCTION 43
Outer membrane vesicles (OMVs) are nano-sized proteoliposomes released from the bacterial 44
cell envelope [1]. OMV release is a highly conserved process, occurring in all growth phases and 45
environmental conditions [2]. OMVs contain and deliver a broad range of cargos, from large 46
hydrophobic molecules to DNA, making them a versatile and generalised form of secretion that 47
enhances bacterial fitness in hostile environments [3-6]. They also contribute significantly to 48
pathogenesis, via the delivery of virulence factors such as toxins, adhesins and 49
immunomodulatory compounds directly into the host cell [7-9]. In a mouse model, purified 50
OMVs from Escherichia coli were sufficient to cause lethal sepsis in the absence of intact 51
bacterial cells, indicating their potency in enhancing infection and inflammatory processes [10]. 52
The immunogenicity and ubiquitous production of OMVs has also led to their clinical use in 53
vaccine preparations [11], representing an application for OMVs in generating immunity against 54
bacterial infections without the risks associated with live cell vaccines. Whilst many virulence 55
factors are known to be OMV cargos, the processes underlying their delivery to host cells during 56
infection are not well characterized. Understanding these mechanisms could help to identify 57
targets for inhibition of OMV-associated toxin delivery and lead to attenuation of bacterial 58
infections, as well as helping to achieve their therapeutic potential in medicine, via vaccines and 59
engineered delivery vehicles [12-14]. 60
Release of OMVs occurs during infection, and has advantages over other secretion 61
systems. They can carry a broad range of cargos, from protein toxins to hydrophobic small 62
molecules such as the Pseudomonas aeruginosa quorum sensing molecule quinolone signal 63
LPS composition impacts OMV entry kinetics | 5
(PQS), and vesicular cargos are protected from environmental insults [15, 16]. In addition, 64
OMV-mediated delivery of virulence factors can function over longer distances than contact-65
dependent secretory pathways [17]. While much is known about the cargos contained within 66
OMVs, the small size of OMVs (20-200 nm) and rapid kinetics of entry (cargo-specific effects 67
can often be detected within minutes) have made studying their interactions with host cells 68
difficult. Previous work has often relied on OMVs labelled with dyes, non-discriminate probes 69
that modify vesicular contents during labeling. While such probes allow real-time analysis of 70
OMV entry and cargo delivery, their potential to modify vesicle components may interfere with 71
the vesicle’s physicochemical characteristics, and alter the mechanism of OMV recognition, 72
entry and cargo release [18-20]. Other approaches rely on immunolabelling of OMV-associated 73
epitopes, but this often requires fixation of cells at pre-determined time points, and makes 74
assumptions about OMV cargo, which may ignore natural sub-populations of OMVs [21]. Some 75
experiments have used specific changes in host cell phenotypes in response to OMV contained 76
toxins as an indicator of OMV uptake [4]. However, such changes in host cell responses have 77
distinct dynamics from the OMV entry event, and allow only indirect conclusions about entry 78
kinetics [22]. These challenges have often lead to discrepancies in observations of OMV entry 79
and cargo delivery [14], demonstrating the need for an assay that can detect OMV entry 80
processes in a consistent and repeatable manner. In this paper we describe a novel assay to 81
continuously measure OMV entry and cargo release to host cells with high sensitivity, and in a 82
format that is adaptable for high throughput screening. Using this assay to study entry of OMVs 83
from different E. coli serotypes and pathovars into host cells, we identified key bacterial and host 84
LPS composition impacts OMV entry kinetics | 6
factors that determine the route of entry, and thereby kinetics and efficiency of vesicular cargo 85
delivery and trafficking. 86
RESULTS 87
A highly sensitive, kinetic assay for monitoring OMV entry into host cells. We set out to 88
develop a highly sensitive and dynamic assay that would allow us to monitor the kinetics of 89
OMV entry into host cells. We used a genetically encoded hybrid reporter probe that is 90
incorporated into the bacterial outer membrane and subsequently targeted to the OMV surface. 91
ClyA, a cytolysin that is sorted into OMVs produced by pathogenic E. coli, acts as the targeting 92
component, and is fused to the TEM domain of β-lactamase (Bla), which acts as an 93
enzymatically active probe (Figure 1A), and prevents assembly of the toxin into its biologically 94
active oligomeric conformation [12]. Host cells were incubated with CCF2-AM, a dye composed 95
of a covalently linked coumarin and fluorescein molecule, resulting in FRET and green 96
fluorescence emission, specifically in the eukaryotic cytoplasm where it is processed by 97
esterases. Esterification decreases the hydrophobicity of the FRET probe, thus decreasing its 98
membrane permeability and trapping the probe in the host cell cytoplasm. When OMVs isolated 99
from the producing bacterial strain enter host cells, their Bla cargo is able to cleave CCF2-AM, 100
abolishing FRET and resulting in a shift in emission from green (530 nm) to blue (460 nm) 101
fluorescence (Figure 1A). We monitored the FRET kinetics upon incubation of OMVs with host 102
cells, and analyzed efficiency of OMV uptake by host cells ([Em460/Em530]t=0hrs)/ 103
[Em460/Em530]t=3hrs). We further analyzed data by fitting to a cubic spline function and 104
estimating gradients to extract maximal rate of entry (rmax) and rate over time (see SI Materials 105
LPS composition impacts OMV entry kinetics | 7
and Methods). Experimental traces were limited to three hours, since beyond this time point the 106
FRET signal decayed, likely due to degradation of the substrate within the host cell cytoplasm. 107
Figure 1. Genetically encoded Bla probes are enriched in E. coli OMVs and retain their enzymatic activity. 108
(A) Expression of genetically encoded Bla probes is induced in bacteria and secreted OMVs are isolated for all 109
subsequent experiments. Entry of OMVs containing Bla probes into host cells can be detected using a continuous 110
FRET assay. (B) Whole cell lysate (WCL), supernatant (sup) and outer membrane vesicles (OMV) fractions isolated 111
from EHEC expressing ClyA-Bla, carrying empty vector, or no vector were separated by SDS-PAGE and 112
expression of ClyA-Bla was detected by Western Blotting and probing with α-Bla antibody. (C) Specific enzyme 113
activity in whole cell lysate, supernatant, OMV or solubilized OMV fractions isolated from EHEC expressing ClyA-114
Bla, Bla-ClyA, or carrying empty vector (data shown are means ± stdev, n=3). 115
116
Genetically encoded Bla probes are targeted to E. coli OMVs and retain their enzymatic 117
activity. First, we set out to verify whether ClyA-Bla fusion constructs retained ClyA’s ability to 118
partition into vesicles, and were indeed targeted to E. coli OMVs. Following induction of probe 119
production, OMVs were isolated from enterohemorrhagic E. coli (EHEC) containing empty 120
vector, or expressing either ClyA-Bla (C-terminal fusion, enzyme exposed on the OMV surface) 121
or Bla-ClyA (N-terminal fusion, enzyme facing the OMV lumen) enzymatic probes. Probe 122
expression did not significantly change cross OMV morphology (Figure S1A ) or charge (mean 123
ζ-potential -6.7 ± 3.6 mV, Figure S1C), but did cause a slight but significant increase in OMV 124
size distribution (~ 20% increase in median diameter; Figure S1B). Probe expression did not 125
appear to result in cell envelope stress, as the amount of OMVs released per cell did not change 126
significantly compared to the untransformed strains (approximately 41 vs 39 vesicles/cell). 127
Sizing data (mean diameter 134 nm, range 10-400 nm across all OMV preparations) were in 128
LPS composition impacts OMV entry kinetics | 8
accordance with previously published data for E. coli OMVs [12]. Intact ClyA-Bla fusion protein 129
was detected in samples from EHEC whole cell lysate, supernatant and OMV fractions (Figure 130
1B), suggesting that the fusion protein was targeted to and enriched in OMVs, as previously 131
reported for non-pathogenic E. coli [12]. The ClyA-Bla probe was oriented with Bla facing the 132
exterior of the OMV, as the protein was gradually degraded during treatment of ClyA-Bla OMVs 133
with papain protease, while the probe remained intact in OMVs containing Bla-ClyA, where Bla 134
faces the vesicle lumen (Figure S1D). The specific enzymatic activity was ~ 3-fold higher for 135
ClyA-Bla OMVs than for Bla-ClyA OMVs with similar activities in whole cell lysates, and both 136
activities were equalized by lysis of vesicles and probe solubilization, suggesting efficient 137
expression of active β-lactamase with the anticipated orientation (inward facing for Bla-ClyA, 138
outward facing for ClyA-Bla) in isolated OMVs (Figure 1C). Average OMV concentration was 5 139
x 1012
particles per ml, and particle concentrations of all samples were normalized to give a 140
consistent OMV concentration for subsequent experiments. 141
142
OMV-targeted Bla probes report on rapid vesicle uptake and dismantling by host cells. 143
Having verified the correct targeting, orientation and enzymatic activities of the Bla probes, we 144
used them to dissect OMV entry (i.e., exposure of ClyA-Bla to cytoplasmic dye) and release of 145
OMV luminal contents (i.e., exposure of Bla-ClyA to cytoplasmic dye) into epithelial cells. We 146
used both Hela (cervical epithelial) and RKO (intestinal epithelial) cells loaded with CCF2-AM 147
dye and exposed to OMVs at an MOI of 1000 OMVs/cell. OMV yield was approximately 27 ± 148
13 OMVs/bacterial cell for the different pathovars used, so this corresponds to a bacterial MOI 149
of approximately 37 bacteria/cell, a dose commonly used in infection assays, or approximately 150
LPS composition impacts OMV entry kinetics | 9
10 µg/ml OMV protein (published assays use between 5-200 µg/ml OMV protein). EHEC ClyA-151
Bla OMVs caused a rapid increase in blue/green fluorescence over the course of a 3 hour 152
experiment. OMVs lacking probe did not cause a significant change in FRET signal. (Figure 2A-153
C). While the rate of cargo release remains stable throughout the experiment (Figure S2B), the 154
rate of entry is initially high but gradually decreases and approaches the rate of cargo release 155
(Figure S2A). OMV entry kinetics are similar in intestinal epithelial (RKO) cells (Figure S3). 156
Results of these kinetic analyses were visually confirmed by capturing FRET of samples at the 157
onset and endpoint of the experiment (Figure 2D). The rapid kinetics inferred from the FRET 158
traces also correlated with rapid internalization and re-distribution of OMV lipid inside host 159
cells, with a significant portion of OMV material localized to an intracellular, tubular structure 160
surrounding the nucleus, likely the ER, even after 10 minutes, the fastest we could feasibly 161
prepare samples for imaging (Figure 2E). These results suggest that our approach is capable of 162
capturing the rapid internalization and dismantling of OMVs, which proceeds too fast to 163
adequately capture by imaging. As the rate limiting step for cargo release appears to be OMV 164
entry, we further focused on analyzing potential determinants of the entry process. 165
166
Figure 2. Reporter OMVs capture rapid kinetics of vesicle uptake by host cells in real time. (A) CCF2-AM 167
loaded Hela cells were exposed to OMVs from EHEC carrying ClyA-Bla (red), or vector control (grey) at an MOI 168
of 1000 for 3 hours. Ratio of blue:green fluorescence) over time was plotted as mean ± stdev (n=3). (B) Rmax was 169
determined from data in S2A to visualize speed of uptake and is shown are means ± stdev (n=3). Significance was 170
determined by analysis of variance, with a Brown Forsythe test to determine equal variance. (**) p≤0.01. (C) 171
Absolute FRET changes after 3 h were determined from data in (A) and plotted as efficiency of OMV uptake. Data 172
shown are means ± stdev (n=3). Significance was determined by ANOVA, with a Brown Forsythe test to determine 173
LPS composition impacts OMV entry kinetics | 10
equal variance. (**) p≤0.01. (D) CCF2-AM loaded Hela cells were imaged by confocal microscopy and merged 174
blue/green images representative of 15 images (n=3) are shown. Scale bars, 20 µm. (E) Hela cells incubated with 175
cellmask orange-labelled OMVs (red) for 10 and 60 min and slice views of z-stacks were acquired by confocal 176
microscopy. Scale bars, 10 µm; 177
178
EHEC OMVs enter host cells more rapidly and efficiently than OMVs from non-179
pathogenic E. coli. Next, we compared the uptake kinetics of OMVs isolated from EHEC and 180
non-pathogenic E. coli K12. Uptake of EHEC OMVs was faster (Figure 3A), and approximately 181
30% more efficient (Figure 3C), compared to K12 OMVs; the maximal rate was higher (Figure 182
3B), and a high rate of uptake was sustained for longer for EHEC than for the K12 strain (Figure 183
S2C). Both rmax (Figure S2D) and uptake efficiency (Figure S2E) increased with increasing 184
OMV concentration for both EHEC and K12, but for K12 vesicles rmax saturated at a lower OMV 185
concentration and a lower uptake efficiency was achieved. Taken together, these results suggest 186
EHEC OMVs contain cargos absent from K12 OMVs that accelerate and sustain the rate and 187
thus increase the efficiency of vesicle uptake by host cells. 188
189
Figure 3. EHEC OMVs enter host cells more rapidly and efficiently than E. coli K12 OMVs. (A) CCF2-AM 190
loaded Hela cells were exposed to OMVs from EHEC (red) or E. coli K12 (blue) carrying ClyA-Bla, at an MOI of 191
1000 for 3 hours. Ratios of blue:green fluorescence over time were plotted as means ± stdev (n=3). Maximum rates 192
(B) were determined from data in Figure S2 and absolute FRET signal changes after 3 hrs (C) were determined from 193
data in (A) and plotted to visualize overall efficiency of uptake for EHEC (red) and K12 (blue) OMVs. Data shown 194
are means ± stdev (n=3). Significance was determined by ANOVA, with a Brown Forsythe test to determine equal 195
variance. (***) p≤0.001, (**) p≤0.01. 196
197
LPS composition impacts OMV entry kinetics | 11
Lipopolysaccharide structure shapes kinetics of OMV uptake by host cells. Since OMVs are 198
derived from the outer membrane of Gram-negative bacteria, they contain lipopolysaccharides 199
(LPS), [23]. Whilst lipid A and the core oligosaccharide regions are well conserved, many 200
species including EHEC contain a highly variable polysaccharide domain known as O antigen 201
[24]. The O antigen constitutes the outermost structural region of LPS, and due to its length of up 202
to 40 nm [24], likely the first component in contact with host cells. These characteristics led us to 203
hypothesize that the O antigen present on EHEC OMVs may be a structural determinant of OMV 204
recognition and uptake by host cells. 205
To test this hypothesis, we carried out FRET assays with Hela cells exposed to ClyA-Bla 206
reporter OMVs harvested from three pairs of strains, reflecting different E. coli serotypes and 207
pathovars and O antigen deficient isogenic mutants, to determine how the presence or absence of 208
O antigen would impact OMV uptake kinetics in each case. OMVs were derived from two 209
different pathovars of E. coli, EHEC (serotype O157) and enteroaggregative E. coli (EAEC, 210
serotype O42), and from the non-pathogenic lab strain K12 (serotype O16). For EHEC, OMVs 211
from O157 wild type cells and an isogenic strain lacking the O157 O antigen (gne::IS629, [25]) 212
were compared (Figure 4). The O antigen deficient mutant gne::IS629 carries a 1310 bp insertion 213
in gne, disrupting the epimerase required for synthesis of the oligosaccharide repeating unit in 214
the O antigen [25, 26], leading to a ~ 10 nm decrease in median OMV diameter (Figure S1B). 215
The rmax for ClyA-Bla reporter OMVs derived from this O antigen deficient EHEC strain and the 216
isogenic wild type O157 strain were not significantly different (Figure 4B). However, OMVs 217
derived from wild type EHEC with intact O antigen sustained a higher entry rate over a longer 218
LPS composition impacts OMV entry kinetics | 12
period (Figure S4A), and thus entered host cells ~ 43% more efficiently than those derived from 219
O antigen deficient EHEC (Figure 4D). 220
OMVs from wild type EAEC (serotype O42, intact O antigen) were compared with an 221
isogenic O antigen deficient mutant (∆wbaC, lacking a glycosyltransferase necessary for O 222
antigen synthesis; [27]). EAEC OMVs with intact O antigen were around 20 nm larger in 223
median diameter than EHEC OMVs, suggesting they carry a longer O antigen, and the diameter 224
dropped in the O antigen deficient mutant, to the same size as EHEC O antigen deficient OMVs 225
(Figure S1B). EAEC OMVs with intact O antigen entered host cells ~ 66% more efficiently than 226
OMVs without O antigen, due to a 77% higher rmax (Figure 4D-F) and a higher sustained rate 227
over time (Figure S4B). 228
The non-pathogenic E. coli K12 strain MG1655 has lost its ability to produce O antigen 229
due to a disruption in wbbL encoding the rhamnosyltransferase required for O antigen synthesis 230
[28]. We compared entry of OMVs from this O antigen deficient strain (median OMV diameter 231
decreased by ~ 10 nm, compared to O16 positive strain), to those from an isogenic strain (DFB 232
1655 L9), where wild type wbbL has been restored, allowing for expression of the strain’s 233
original O16 O antigen [27]. Similar to O157, the presence or absence of O antigen did not alter 234
rmax, but the presence of O antigen allowed for a higher rate to be sustained for longer (Figure 235
S4C), leading to a ~ 22% higher efficiency overall (Figure 4G-I). A similar effect of O antigen 236
on uptake kinetics was observed in intestinal epithelial cells (Figure S3). Taken together, these 237
results suggest that the presence of the LPS O antigen increases the entry efficiency of OMVs 238
into host cells, independent of the specific mutation leading to O antigen deficiency. Depending 239
on the serotype used, this is caused by enhancing rmax and/or by sustaining a higher uptake rate 240
LPS composition impacts OMV entry kinetics | 13
over a longer period, compared to OMVs lacking O antigen. These variations may be due to 241
differences in physicochemical features and/or other vesicle cargos between the different 242
serotypes. 243
244
Figure 4. LPS structure affects rate and efficiency of OMV uptake by host cells. CCF2-AM loaded Hela cells 245
were exposed to ClyA-Bla OMVs isolated from EHEC (serotype O157, A-C), EAEC (serotype O42, B-F) or K12 246
(serotype O16, G-I) containing O antigen (red), or lacking O antigen (blue), at an MOI of 1000 for 3 hours. Ratios of 247
blue:green fluorescence over time (A, D, G) were plotted as means ± stdev (n=3). Maximum rates (B, E, H) were 248
extracted from data in Figure S4 and absolute FRET changes after 3 hrs (C, F, I) were determined from data shown 249
in A, D and G. Data shown are means ± stdev (n=3); Significance was determined using ANOVA, with a Brown 250
Forsythe test to determine equal variance. (***) p≤0.001, (**) p≤0.01, (*) p≤0.05, (ns) not significant. 251
252
LPS structure determines the preferred entry route of OMVs into host cells. Next, we 253
evaluated the relative contribution of cellular trafficking pathways to OMV uptake and 254
determined if this was affected by LPS structure. Inhibition of macropinocytosis following 255
treatment of host cells with 20 uM blebbistatin enhanced both the rate and efficiency of uptake in 256
the strains with shorter O antigen (EHEC and K12) and left it unaltered for EAEC (Figure S5). 257
These data suggest that only a small fraction of OMVs usually enters cells by micropinocytosis, 258
and inhibition of this relatively slow uptake route either does not affect or accelerates uptake. 259
Next, we tested if OMV uptake required dynamin, using the dynamin GTPase inhibitor dynasore. 260
Treatment of host cells with dynasore completely abolished uptake of OMVs, independent of 261
serotype and the presence of O antigen (Figure S5). Next, we determined whether OMV uptake 262
was via clathrin-coated pits, or via lipid raft-mediated endocytosis, both of which require 263
dynamin [29-31]. We inhibited clathrin-mediated endocytosis, either by proteolytic removal of 264
LPS composition impacts OMV entry kinetics | 14
all protein receptors from host cells with papain prior to OMV incubation, or by blocking pit 265
assembly using chlorpromazine [32]. Removal of protein receptors from the host cell surface 266
increased uptake rate (Figure S6) and efficiency (Figures 5 and S6) for OMVs with O antigen, 267
but decreased or abolished uptake rate and efficiency of O antigen deficient OMVs. In general, 268
both papain and chlorpromazine treatment decreased the uptake of O antigen negative OMVs 269
but, although they had variable effects, they did not reduce uptake of O antigen positive OMVs 270
(Figures 5 and S6). This suggests that OMVs lacking O antigen require protein receptors for 271
uptake and use clathrin-mediated endocytosis as a main route of entry. In contrast, OMVs with 272
intact O antigen do not rely on protein receptors for entry, and inhibition of clathrin-mediated 273
endocytosis does not prevent their uptake into host cells. 274
275
O antigen containing OMVs enter host cells faster because they can access raft-mediated 276
endocytosis more efficiently. Since OMVs displaying O antigen on their surface accessed host 277
cells faster in the absence of clathrin-dependent endocytosis, we investigated whether this was 278
mediated by raft-dependent pathways. Disruption of raft-mediated endocytosis, either by 279
sequestration of membrane cholesterol from membrane microdomains via methyl-β-cyclodextrin 280
or by disrupting raft dynamics with filipin [33], led to a reduced rmax (Figure 5) and uptake 281
efficiency (Figure S6). These data show that, while OMVs are able to access different uptake 282
routes including macropinocytosis, clathrin-dependent and raft-dependent endocytosis, OMVs 283
displaying O antigen on their surface are able to access raft-dependent endocytosis more 284
efficiently, while OMVs lacking O antigen are more reliant on clathrin-mediated uptake (Figure 285
6). Shifting a larger fraction of O antigen-positive OMVs to raft-mediated endocytosis further 286
LPS composition impacts OMV entry kinetics | 15
accelerates their uptake, and we conclude the differences in uptake routes driven by LPS 287
structure account for differences in uptake rate and efficiency we observe. 288
289
Figure 5. OMVs lacking O antigen are biased towards clathrin-mediated endocytosis, while OMVs with O 290
antigen can efficiently access host cells via lipid rafts. Hela cells were either left untreated (control, red), or pre-291
treated with 5 µg/ml papain (lilac), 1 µg/ml chlorpromazine (pink), 5 mM methyl-β-cyclodextrin (light green) or 1 292
µg/ml filipin (turquoise), and exposed to ClyA-Bla OMVs isolated from EHEC (A), EAEC (B) or K12 (C) with or 293
without O antigen at an MOI of 1000 for 3 hours. Total FRET changes after 3 hrs were determined from data in 294
Figure S6 and data shown are means ± stdev (n=3). Significance compared to the control group was determined 295
using ANOVA, with a Brown Forsythe test to determine equal variance. (***) indicates p≤0.001, (**) p≤0.01, (*) 296
p≤0.05, (ns) not significant. 297
298
Figure 6. LPS composition determines major route and kinetics of OMV entry into host cells. Whilst it is well 299
established that pathogenic species utilize OMVs during infection, the specific adaptations which allow OMVs to 300
contribute to pathogenesis require further exploration. This work has developed a new approach to overcome current 301
methodological limitations and provide consistent data for future studies and allow new insights into the interactions 302
of OMVs with host cells during infection. This method has shown the relevance of LPS composition, in particular 303
the presence of O antigen, in determining the entry route and kinetics of OMVs. Further work in this area may 304
reveal targets for inhibition of these processes, and enable attenuation of infections by preventing the OMV-305
associated delivery of virulence factors. 306
307 308
DISCUSSION 309
Interactions between bacterial outer membrane vesicles and epithelial cells are now recognized 310
as an important driver of bacterial pathogenesis. Yet, our ability to study vesicle-host cell 311
interactions has been limited by a lack of methods to capture the rapid kinetics of vesicle entry 312
and dismantling in real-time, and without altering the physicochemical properties of the vesicle. 313
Here we describe a novel assay that fulfils these requirements and allowed us to study the 314
kinetics of OMV uptake with enough temporal resolution to reveal critical differences in rate and 315
LPS composition impacts OMV entry kinetics | 16
uptake efficiency of vesicles derived from different E. coli serotypes and pathovars. The method 316
uses a genetically encoded, OMV targeted probe and a cell-permeable dye, resulting in a change 317
in FRET upon reporter uptake and dye cleavage. Advantages of this system include its high 318
sensitivity (5 µg/ml OMVs, the lowest concentration reported in the literature, produced a 319
reproducible trace with good signal/noise ratio) and rapid response (signal was detected within 320
seconds). A potential drawback is, that it is not known if the ClyA-Bla probe is expressed 321
equally across the entire OMV population, but this is equally true for other markers and assays 322
currently in use. The system’s use can be extended to a high-throughput format, allowing further 323
study of bacterial and host factors determining OMV uptake and trafficking. Using a transwell 324
format, the method can be applied to cell-based assays consisting of bacteria releasing OMVs, 325
and host cells without the need for OMV isolation. Although the specific probes used here were 326
functional across a range of E. coli isolates and different host cell types, their use in other 327
bacterial species will require further characterization to determine if they are targeted to OMVs 328
and retain correct orientation and enzymatic activity. 329
We selected EHEC and EAEC OMVs for this study, since OMVs have been shown to 330
play a crucial role in toxin stabilization and delivery for both pathovars [34, 35], and have been 331
considered as a means to vaccinate and protect against hemolytic uremic syndrome, a severe 332
complication of EHEC infection [36]. It is clear that LPS, and specifically O antigen, contributes 333
to bacterial within-host fitness and pathogenicity, by enhancing resistance to complement, 334
modulating phagocytosis and phage infection [37, 38]. The O antigen of most E. coli strains has 335
10-18 repeats, but can exceed 80 repeats [39, 40]. The length of the O antigen is equally variable 336
LPS composition impacts OMV entry kinetics | 17
(~5-50 nm), and is positively correlated with the ability of the bacterial cell to adhere to host 337
cells and tissues, while loss of O antigen results in defects in colonisation, biofilm formation, and 338
increased pathogen clearance [24, 41-43]. Recent work showed that EHEC OMVs allow 339
efficient delivery of LPS into the host cell cytoplasm, resulting in inflammatory responses, 340
caspase-11 activation and cell death, but did not explore the role of LPS in uptake [44]. Our data 341
suggest that O antigen has an additional, previously unrecognized role during bacteria-host 342
interactions, which is to steer OMVs towards raft-mediated endocytosis, accelerating uptake and 343
delivery of vesicle associated virulence factors such as hemolysins and Shiga-like toxins [45] to 344
host cells and enhancing pathogenicity. 345
It is well known that OMVs contain different cargos, depending on pathovar and serotype 346
[46]. This means the comparison of O antigen deficient mutants with wild type OMVs as well as 347
comparison of different pathovars has the pitfall that other vesicle cargos may be modulated and 348
alter uptake kinetics. To dissect the effect of O antigen independent of other cargos, we 349
attempted to deplete O antigen of wild type OMVs by treatment with a glycoside hydrolase, but 350
found enzymatic activity was not limited to O antigen cleavage but modified the core LPS as 351
well. However, we observed a strong correlation between O antigen and uptake kinetics across 352
three different serotypes and pathovars, suggesting that O antigen is, if not the only factor, at 353
least a key determinant of uptake kinetics. Since EAEC OMVs showed the most distinct change 354
in entry kinetics upon O antigen deletion, with rmax impacted as well as rate sustenance and 355
efficiency (Figure 4) and O42 antigen seemed to be much longer than EHEC O157 or K12 O16 356
LPS composition impacts OMV entry kinetics | 18
antigens, which seemed similar in size and displayed similar changes upon O antigen deletion 357
(Figure S1B), we speculate that O antigen length may impact maximal entry rate. 358
We used our newly-devised assay to identify the relative contribution of cellular uptake 359
pathways to OMV entry into host cells. Clathrin- and raft-dependent endocytosis, 360
macropinocytosis and membrane fusion have all previously been reported as uptake pathways for 361
bacterial OMVs, and it is likely that discrepancies between studies result, at least in part, from 362
differences in species, strains and methodology used to study uptake [48]. Uptake of OMV cargo 363
by fusion of vesicles with the host cell membrane can be ruled out as a major route of uptake for 364
OMVs used in our study, since in this case ClyA-Bla would be exposed on the outer leaflet of 365
the host cell membrane and would not account for the rapid cleavage of the cytoplasmic FRET 366
dye. Assays using pharmacological inhibitors to block specific endocytic pathways, showed that 367
while all OMVs use multiple uptake routes, their surface structure biases them towards different 368
pathways. For example, O antigen deficient OMVs had a stringent requirement for surface 369
protein receptors for their uptake, while O antigen containing OMVs were able to access protein-370
receptor independent pathways. Depletion of such receptors actually allowed them to access 371
protein-receptor independent pathways more efficiently and utilize raft-mediated endocytosis, a 372
more rapid mode of uptake, as main route of entry. While raft-mediated endocytic routes are not 373
as well characterized as clathrin-mediated endocytosis, it is clear there are multiple pathways, 374
including caveolin and non-caveolin dependent raft-mediated endocytosis. Our experiments 375
suggest that the entry of O antigen containing OMVs is raft- and dynamin dependent, but 376
protein-receptor independent, and no co-localization between OMVs and caveolin was detected. 377
LPS composition impacts OMV entry kinetics | 19
The requirement of dynamin is likely, based on complete inhibition of uptake following 378
treatment with dynasore, however this is confounded by the dual inhibitory effect of dynasore 379
both on dynamin as well as cholesterol containing micro domains [49]. A recent study focusing 380
on vesicular cargo delivery of EHEC OMVs to host cells over longer time frames also concluded 381
that OMVs enter host cells via dynamin-dependent endocytosis [45]. We therefore conclude they 382
use a raft-mediated, and likely dynamin dependent, but protein-receptor and caveolin-383
independent route of uptake, and the detailed requirements regarding their uptake are subject to 384
current studies. 385
MATERIALS AND METHODS 386
Strains and growth conditions 387
The strains used in this study were the E. coli serotype O157:H7 strain Sakai 813, a derivative of 388
enterohaemorrhagic E. coli (EHEC) RIMD 0509952, and its O antigen deficient derivative, MA6 389
(∆gne, [25]; the E. coli serotype O42 wild type strain (an enteroaggregative E. coli isolate, [47], 390
and its isogenic, O antigen deficient derivative strain (∆wbaC, [27]; the E. coli serotype O16 391
strain DFB 1655 L9 (a K12 strain containing a restored wbbL gene), and its isogenic, O antigen 392
deficient derivative, MG1655 [27]. All strains were transformed with plasmids pBAD ClyA-Bla, 393
Bla-ClyA, or empty vector (a kanR derivative of the pBAD amp
R vector provided by Matthew 394
DeLisa, Cornell University), [12]. Strains were grown in LB containing 50 μg/ml kanamycin, at 395
37 °C with shaking at 200 rpm. 396
397
Isolation of outer membrane vesicles by ultracentrifugation 398
LPS composition impacts OMV entry kinetics | 20
100 ml cultures were grown in LB at 37 ºC, with agitation at 200 rpm. Once the OD600 reached 399
0.5-0.6, expression of ClyA-Bla was induced with 0.2% L-arabinose and grown for a further 16 400
h. Cells were then pelleted at 6000xg, and the supernatants were removed and filtered with a 401
0.45um syringe filter. Aliquots of filtered supernatants were spread on LB agar and grown 402
overnight at 37 ºC to check that all viable cells had been removed by filtration. 25 ml of filtered 403
supernatants were centrifuged in a Beckman XL90 ultracentrifuge using a 70Ti rotor at 404
100,000xg (30,000 rpm) for 2 h at 4 °C. After centrifugation, supernatants were removed, and the 405
OMV pellets were resuspended in 1 ml colorless DMEM or sterile water (for TEM) and stored at 406
-20 °C. 407
Detection of Bla probes in cellular fractions 408
12 μl of samples normalized for their protein content from EHEC ClyA-Bla and Bla-ClyA whole 409
cell lysate, supernatant and OMV fractions were added to 3μl 5X SDS loading dye and boiled for 410
10 min. Samples were loaded onto a 15 well BioRad pre-cast stain-free SDS-PAGE gel and run 411
at 120V, 200mA for 45 min. The gel was then transferred onto a PVDF membrane in transfer 412
buffer containing 20% methanol for 80 minutes at 100V. After transfer, the membrane was 413
blocked at room temperature in TBS 0.1% Tween-20 and 5% skim milk for 1h with agitation. 414
The membrane was washed 3 times with TBS 0.1% Tween-20 (5 min per wash). After blocking, 415
the membrane was incubated with a 1:2000 dilution of mouse anti-Bla primary antibody in TBS 416
0.1% Tween-20 and 5% skim milk overnight at 4 ºC with agitation. The following day, the 417
membrane was washed 3 times as before, and incubated with a 1:5000 dilution of sheep anti-418
mouse secondary antibody in TBS 0.1% Tween-20, 5% skim milk for 1h at room temperature 419
LPS composition impacts OMV entry kinetics | 21
with agitation. The membrane was washed again 3 times, and 2 ml BioRad ECL reagents were 420
added to the membrane and incubated for 5 min, before visualization with a BioRad ChemiDoc 421
imager. 422
423
Nitrocefin assay to determine β-lactamase activity 424
50 μl of samples were added in triplicate to a 96-well plate. Nitrocefin was diluted to 0.5 mg/ml 425
in PBS and 50 μl was added to each sample. The absorbance at 486 nm was measured in the 426
FluoStar Omega plate reader for 2 h, and the change in absorbance over time was used to 427
determine the specific activity in samples, using the protein concentration determined by the 428
CBQCA kit. 429
430
Protein Quantitation 431
To quantify levels of protein in cell fractions, the ThermoFisher CBQCA Protein Quantitation kit 432
was used according to the manufacturer’s instructions. 433
434
Papain and detergent treatment of OMVs 435
Triton X-100 and SDS were added at a concentration of 1% to 20 μl OMVs for 45 min at 37 ºC. 436
5ug/ml papain was then added for 30 or 60 min at 37 ºC. The papain reaction was inactivated 437
using 1 mM PMSF at room temperature for 30 min. 5 μl SDS-PAGE loading dye was added to 438
the samples, which were then boiled for 10 min. Samples were run on a 15-well pre-cast stain 439
LPS composition impacts OMV entry kinetics | 22
free gel for 45 min at 120V, and then subjected to Western blotting with anti-β-lactamase 440
primary antibody (Pierce) as described above. 441
442
Plate reader FRET experiments 443
HeLa cells (passage 1-7) were seeded in triplicate in a black-walled, clear bottom 96-well plate 444
at a concentration of 1x105 cells per ml in Dulbecco’s modified Eagle medium (DMEM) 445
supplemented with 1% L-glutamine, 1% Penicillin/Streptomycin and 10% heat inactivated fetal 446
bovine serum. The plate was incubated at 37 ºC, 5% CO2 for 24 h prior to experiments. The 447
following day, cells were loaded with 20 μl 6X CCF2-AM with 100 μl colourless 448
unsupplemented DMEM (cDMEM) and incubated at room temperature for 1 h in the dark to 449
allow dye loading. The dye was removed by washing 2x in PBS and 1x in cDMEM. Cells were 450
treated with 5 mM methyl-ß-cyclodextrin or 1 μg/ml filipin to inhibit cholesterol mediated 451
endocytosis, 80 uM Dynasore for dynamin inhibition, or 20 uM blebbistatin for 452
macropinocytosis inhibition for 1h at 37 ºC. Cells were treated with 1 μg/ml chlorpromazine for 453
1h at 37 ºC to inhibit formation of clathrin-coated pits, or with 5 μg/ml papain for 15 min at 37 454
ºC to remove surface proteins, before inactivation of papain with 5 mM PMSF for 20 min. 455
Reporter OMVs were diluted in cDMEM and added to the cells for a final concentration of 10 456
μg/ml, or 1x108 vesicles, corresponding to an MOI of 1000. The plate was immediately placed in 457
the PheraStar plate reader, with excitation at 405 nm and simultaneous dual emission at 530 nm 458
and 460 nm. The wells were scanned (bottom optic) with orbital averaging for a total of 150 459
cycles, equating to a measurement every 90 seconds for 3 hours. The ratio of blue to green 460
LPS composition impacts OMV entry kinetics | 23
fluorescence intensity detected in the cells at each cycle was calculated using GraphPad Prism, 461
and ratios for uninfected, dye-loaded cells were used as the baseline value for each cycle. All 462
traces were normalized to 0 for their first ratio value. All experiments were performed with a 463
minimum of three technical replicates and three independent repeats. 464
465
Efficiency of uptake and statistical analysis 466
Efficiency of uptake was calculated as the absolute change in blue:green fluorescence intensity 467
ratio between 0 and 3 hours ([Em460/Em530]t=0hrs)/ [Em460/Em530]t=3hrs). Analysis of variance 468
(ANOVA) was used to determine statistical significance, with a Brown Forsythe test to 469
determine equal variance (GraphPad Prism software). A p-value of <0.05 was considered 470
statistically significant. 471
472
473
Rate estimation and statistical analysis 474
To estimate the gradients of the data, polynomials were fitted to each data set using the cubic 475
spline function csaps in Matlab. Numerical estimates of the gradients of the resulting 476
polynomials were determined using the gradient function. To ensure that the gradient estimates 477
were as smooth as possible whilst also retaining the overall shape and trend of the data, a small 478
smoothing parameter was used. Analysis of variance (ANOVA) was used to determine statistical 479
significance, with a Brown Forsythe test to determine equal variance (GraphPad Prism software). 480
A p-value of <0.05 was considered statistically significant. 481
482
LPS composition impacts OMV entry kinetics | 24
Confocal Microscopy 483
HeLa cells (P3-7) were seeded on 13mm coverslips in a 12-well plate at a concentration of 1x105 484
cells per ml in complete DMEM, 24 h prior to experiments. The following day, cells were 485
washed and loaded with 100 μl 6X CCF2-AM dye with 500 μl colourless unsupplemented 486
DMEM, and incubated in the dye solution for 1 h at room temperature in the dark. Cells were 487
then incubated with ClyA-Bla reporter OMVs for 0-4 h. The cells were washed with PBS and 488
then fixed with 0.5 ml 4% PFA. The next day, coverslips were mounted onto slides with a drop 489
of Gold Anti-Fade mounting solution and then imaged using a Nikon A1R confocal microscope 490
(Birmingham Advanced Light Microscopy Facility), and fluorescence was observed from 491
excitation at 409 nm and dual emissions at 450 nm and 520 nm. Z stacks were produced with 492
gain, slice thickness, exposure and laser intensity kept the same for all slides, and images were 493
taken for 3 representative fields of view per slide and n=3 independent samples. The Z stacks 494
were converted to maximum intensity projection images. For OMV localization experiments, 495
OMVs were stained using cell mask orange (1:500) for 1 h at 22 °C and gentle agitation. 496
Following staining, samples were washed with 28 volumes of PBS and labelled OMVs pelleted 497
by ultracentrifugation (100,000xg, 2h). Hela cells were exposed to labelled OMVs for 10 of 60 498
minutes prior to fixation in 3.2% formaldehyde. Slides were imaged using an Olympus IX83 499
inverted microscope fitted with a FV3000 confocal system and 100x Super Apochromat oil 500
objective. Images were captured using Olympus Fluoview software and processed using the 501
CellSens extension package. 502
503
LPS composition impacts OMV entry kinetics | 25
ACKNOWLEDGMENTS 504
We thank Matthew DeLisa (Cornell Univ.) for providing ClyA-Bla and Bla-ClyA plasmid 505
constructs and Peter Feng (FDA, Maryland) for providing the EHEC MA6 strain. We thank staff 506
at the Birmingham Electron Microscopy Facility for technical help with sample preparation and 507
transmission electron microscopy. 508
509
LPS composition impacts OMV entry kinetics | 26
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646
LPS composition impacts OMV entry kinetics | 31
SUPPORTING INFORMATION LEGENDS 647
Figure S1. Morphology, size, charge and probe orientation of reporter OMVs. (A) Electron 648
micrographs of negative stained OMV fractions from EHEC wt (left image) or EHEC ClyA-Bla 649
(centre and right images). Scale bars, 0.5 µm. (B) Isolated OMVs were diluted 1x10-6
fold and 650
nanoparticle tracking analysis was used to determine the size distribution. Black lines represents 651
median size from at least 200 tracks acquired per sample. Statistical significance was determined 652
by ANOVA, with a Brown Forsythe test to determine equal variance. (***) p≤0.005, (ns) not 653
significant. (C) ζ-potentials of isolated OMVs. Values represent means from 30 readings per 654
sample. (D) OMV fractions from EHEC expressing Cly-Bla, Bla-ClyA or carrying empty vector 655
were treated with papain for 30 or 60 minutes, and used for Western Blotting with α-Bla 656
antibody. 657
658
Figure S2. Rates of uptake/dismantling and concentration dependency of uptake kinetics 659
for OMVs. (A) CCF2-AM loaded Hela cells exposed to EHEC OMVs carrying ClyA-Bla (red), 660
or empty vector (grey) at an MOI of 1000 for 3 h. Rate of uptake over time was extracted from 661
data in Figure 2A and data shown are means ± stdev (n=3). (B) FRET change upon exposure of 662
Hela cells to EHEC OMVs carrying ClyA-Bla (reporting on exposure to OMV surface to 663
cytoplasm) or Bla-ClyA (reporting on exposure of luminal cargo to cytoplasm). (C) Hela cells 664
were exposed to EHEC or K12 ClyA-Bla OMVs at an MOI of 1000 for 3 hours. Rates of uptake 665
over time were extracted from data in Figure 3A and are means ± stdev (n=3). (D) Experiments 666
were repeated as above but using different OMV concentrations (0-20 μg/ml of protein, 667
corresponding to an MOI of 0- 2000), and maximum rates (D) and efficiency of uptake (E) 668
determined as described above. Data are means ± stdev (n=3). 669
670
Figure S3. Uptake for OMVs from serotypes O157, O42 and O16 with or without O 671
antigen. CCF2-AM loaded RKO intestinal epithelial cells were exposed to OMVs from EHEC 672
LPS composition impacts OMV entry kinetics | 32
O157 (A), EAEC O42 (B), and K12 O16 (C), with O antigen (red) and without O antigen (blue), 673
at an MOI of 1000 for 3 hours. FRET changes (blue/green fluorescence, A-C) and efficiency of 674
uptake (total change over three hours, D) are shown as means ± stdev (n=3). 675
676
Figure S4. Rates of uptake for OMVs from serotypes O157, O42 and O16 with or without 677
O antigen. CCF2-AM loaded Hela cells were exposed to OMVs from EHEC O157 (A), EAEC 678
O42 (B), and K12 O16 (C), with O antigen (red) and without O antigen (blue), at an MOI of 679
1000 for 3 hours. Polynomials were fitted to each data set using the cubic spline function csaps 680
in Matlab. Numerical estimates of the gradients of the resulting polynomials were determined 681
using the gradient function. Data shown are means ± stdev (n=3). 682
683
Figure S5. Effect of blebbistatin and dynasore on uptake of OMVs. Hela cells were either 684
left untreated or pre-treated 80 uM Dynasore for dynamin inhibition (grey), or 20 uM 685
blebbistatin for macropinocytosis inhibition (orange) for 1h at 37 °C and exposed to ClyA-Bla 686
OMVs isolated from EHEC (A, B), EAEC (C, D), or K12 (E, F) at an MOI of 1000 for 3 hours. 687
The FRET signal (ratio of blue:green fluorescence) over time was plotted as mean ± stdev (n=3). 688
689
Figure S6. Effect of pharmacological treatments on OMV uptake. Hela cells were either left 690
untreated or pre-treated with 5 ug/ml papain (lilac), 1 ug/ml chlorpromazine (pink), 5mM 691
methyl-β-cyclodextrin (light green) or 1μg/ml filipin (turquoise) and exposed to ClyA-Bla 692
OMVs isolated from EHEC (A, B), EAEC (C, D), or K12 (E, F) at an MOI of 1000 for 3 hours. 693
The FRET signal (ratio of blue:green fluorescence) over time was plotted as means ± stdev 694
(n=3). 695
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