hplc analysis - dragonflycbd · hplc chromatogram no. s/180718/5 hplc controlled substances...

HPLC analysisno. S/180718/5

CBDA CBD CBN THC THCA

ND 10.9466 % <0.0001 % ND ND

PBG.RO S.R.L.reg. RO 383 09 420

hq@pbg.global+359 877 456 788

Client: Dragonfly Biosciences LtdAddress: 86-90 Paul Street, London EC2A 4NE

Product: 10% CBD oilBatch: 0418

Report issue date: 17/08/2018

main cannabinoids

CBG THCV CBC CBL 1.1748 % ND ND 0.9114 %

other cannabinoids

HPLC controlled substances analysis carried out strictly following the“Recommended methods for the identifcation and analysis of cannabis and cannabis products”

by United Nations Office on Drug and Crime

P. Penchev, PhD chemical engineerHead of production

Ervin Ivanov, M.D., PhD cand.Head of research and development

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HPLC chromatogramno. S/180718/5

HPLC controlled substances analysis carried out strictly following the“Recommended methods for the identifcation and analysis of cannabis and cannabis products”

by United Nations Office on Drug and Crime

P. Penchev, PhD chemical engineerHead of production

Ervin Ivanov, M.D., PhD cand.Head of research and development

NB: The HPLC methods we use are specifically designed to test PBG Laboratories’ production extracts with hemp’s specific compound matrix. As such, any added solvent or carrier agent such as MCT, hemp seed oil or other will introduce their own compound matrixes that sometimes interfere with the results. Our analytical laboratory specialists warn that they detect anomalies in the chromatograms with the introduction of such unidentifiable with our methods compounds!

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HPLC calibration curvesno. S/180718/5

P. Penchev, PhD chemical engineerHead of production

Ervin Ivanov, M.D., PhD cand.Head of research and development

Component Name: CBDA

Curve type: Linear

Equation: y = 1.42797E+07x + 1.14933E+04

Curve Statistics:

Intercept: 1.14933E+04

Slope: 1.42797E+07

r²: 0.999309

Component Name: CBG

Curve type: Linear

Equation: y = 1.05099E+07x + 3.59830E+03

Curve Statistics:

Intercept: 3.59830E+03

Slope: 1.05099E+07

r²: 0.999711

Component Name: THCA

Curve type: Linear

Equation: y = 1.12066E+07x + 8.49547E+02

Curve Statistics:

Intercept: 8.49547E+02

Slope: 1.12066E+07

r²: 0.999551

Component Name: CBC

Curve type: Linear

Equation: y = 4.09714E+07x + 1.54720E+04

Curve Statistics:

Intercept: 1.54720E+04

Slope: 4.09714E+07

r²: 0.999667

Component Name: delta9-THC

Curve type: Linear

Equation: y = 1.01664E+07x + 2.07312E+03

Curve Statistics:

Intercept: 2.07312E+03

Slope: 1.01664E+07

r²: 0.999895

Component Name: CBN

Curve type: Linear

Equation: y = 2.96240E+07x + 1.69928E+04

Curve Statistics:

Intercept: 1.69928E+04

Slope: 2.96240E+07

r²: 0.999356

Component Name: THCV

Curve type: Linear

Equation: y = 9.36954E+06x + 3.03611E+03

Curve Statistics:

Intercept: 3.03611E+03

Slope: 9.36954E+06

r²: 0.999697

Component Name: CBD

Curve type: Linear

Equation: y = 1.30646E+07x - 6.17174E+03

Curve Statistics:

Intercept: -6.17174E+03

Slope: 1.30646E+07

r²: 0.999574

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