hematopoeisis vibs 443/602. peripheral blood smear (may-grunwald-giemsa) 113

HematopoeisisVIBS 443/602

Peripheral blood smear (May-Grunwald-Giemsa)113

Peripheral blood smear (Leishman-Giemsa)eosinophil, basophil, and neutrophils

110

Bone marrow 1976119761

Bone marrow smear; low power view, showing negative images of fat globules

Megakaryocyte: contain many joined nuclei. Each probably contain 8-32 times a normal diploid amount of DNA

1. Nuclei of megakaryocyte

2. Red blood cell

Macrophage: contains greenish, iron-containing granules due to poor quality. Differentiating cells have been torn away during preparation.

1. Macrophage

2. Lymphocyte

3. Neutrophil

1. Pronormoblast

2. Basophilic normoblast

3. Polychromatophilic normoblast

4. Orthochromatic normoblast

5. Myelocyte

6. Late metamyelocytes

1. Pronormoblast

2. Basophilic normoblast

3. Polychromatophilic normoblast

4. Orthochromatic normoblast

5. Myelocyte

6. Late metamyelocytes

1. Late promyelocyte

2. Late myelocyte/early metamyelocyte

3. Nearly mature PMN

1. Smudge cell

2. Promyelocyte

3. Myelocyte

4. Metamyelocytes

1. Pronormoblast

2. Metamyelocytes

3. Band cell

Bone marrow: EM 12a

• Reticular cell

• Developing red blood cell

Bone marrow; EM 8a

1. Lymphocyte

2. PNM

3. Red blood cell

4. eosinophil

Bone marrow; EM 12b

1. PMN

2. Immature neutrophil

3. Red blood cell

Routine H&E of bone

• Marrow spicules

• Lipid in fat cells

H&E of bone marrow

1. megakaryocytes

19761

H&E of bone marrow:

• Red blood cell

Rib 420

Rib 420

Tibia, fetal421

32583

32583

Red bone marrow smear (May-Grunwald-Giemsa)112

Pronormoblastround nucleus and thin rim of deep blue cytoplasm

Basophilic normoblastnuclear condensation ("fractured nucleus") with a deep blue cytoplasm

Cells of Erythropoisesis

Polychromatophilic normoblastcytoplasm has accumulated enough hemoglobin so that it is no longer blue but a bluish-gray to bluish-red

Orthochromatic normoblastmust have an almost mature cytoplasm with only the faintest hint of blue: the nucleus is fully condensed

Reticulocyteno nucleus, mature cytoplasm with blue tint

Red blood celltypical of mature cell seen in circulation

Myelocytecytoplasm becomes increasingly mature (reddish/clear) but still contains blue, nucleus of variable shape

Cells of Granulopoiesis

Promyelocyteoval nucleus with deep blue cytoplasm and azurophilic granules

Metamyelocytedeeply indented nucleus with nearly mature cytoplasm (no longer blue)

Band cellnucleus just prior to lobulation with fully mature cytoplasm

Neutrophil (mature)lobulated nucleus with fully mature cytoplasm