genomic instability in cancer and aging
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Genomic instability in cancer and agingJan Vijg, University of Texas Health Science Center, San Antonio, Texas
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DNA mutations
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Role of genome instability in agingEvolutionary logic of genome instabilityEvidence for genome deterioration in agingAssociation of DNA repair defects with accelerated aging
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Effect of deleterious mutations on fitness in E. coliElena & Lenski, 1997, Nature 390: 395
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The problem in this theory is that of developing a quantitative measure of mutations in somatic cells
Howard J. Curtis, 1963
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LacZ plasmid recovery
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Spontaneous mutant frequencies with age in heart and small intestine
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Spontaneous mutant frequencies with age in mouse organs
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Mutant spectra in vivo and in vitro
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Point mutational spectra in organs from Young (3m) and Old (32m) miceMutant frequency (x10-5)PercentageBrainHeartLiverSpleenSmall intestineMock recoveryLymphomaMutant frequency (x10-5)PercentageOY
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Mutant frequencies in vivo and in vitro
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MEF 6Growth curveMutant frequencyspectra3%20%Total MFPoint MutSize change
Chart2
00
1.32048708091.3204870809
3.62985732253.3407818431
5.84605347784.9668315359
7.99769585245.7037971301
9.57300818317.042934515
11.32003746068.0286077335
12.83941161979.0209082086
14.547230868213.8868270232
17.664925910916.9537772671
20.4212375939
Embryo 6- 3% O2
Embryo 6-20% O2
Days
Population Doublings
Sheet3
Cell typeDateDay# totalPD#total
Embryo 5-3% O223-May-9800
Embryo 5-3% O226-May-9831.2187538443
Embryo 5-3% O229-May-9863.3375565223
Embryo 5-3% O22-Jun-98105.531853742
Embryo 5-3% O26-Jun-98147.5521485042Sum of PD#totalDay# total
Embryo 5-3% O29-Jun-98179.1952350514Cell type036101114172124283438
Embryo 5-3% O213-Jun-982111.27361717Embryo 5-3% + UV01.21875384433.33755652235.5318537425.93239167167.004341513510.109796108311.936259853314.775547638616.9497409717
Embryo 5-3% O216-Jun-982413.0685528328Embryo 5-3% O201.21875384433.33755652235.5318537427.55214850429.195235051411.2736171713.068552832815.078138881717.795495431920.1393769679
Embryo 5-3% O220-Jun-982815.0781388817Embryo 6- 3% + UV01.32048708093.62985732255.84605347786.18633088267.326318599910.466006370912.135033136414.938260172817.3738887669
Embryo 5-3% O226-Jun-983417.7954954319Embryo 6- 3% O201.32048708093.62985732255.84605347787.99769585249.573008183111.320037460612.839411619714.547230868217.664925910920.4212375939
Embryo 5-3% O230-Jun-983820.1393769679
Embryo 5-3% + UV23-May-9800
Embryo 5-3% + UV26-May-9831.2187538443
Embryo 5-3% + UV29-May-9863.3375565223
Embryo 5-3% + UV2-Jun-98105.531853742
Embryo 5-3% + UV3-Jun-98115.9323916716
Embryo 5-3% + UV9-Jun-98177.0043415135
Embryo 5-3% + UV16-Jun-982410.1097961083
Embryo 5-3% + UV20-Jun-982811.9362598533
Embryo 5-3% + UV26-Jun-983414.7755476386
Embryo 5-3% + UV30-Jun-983816.9497409717
Embryo 6- 3% O223-May-9800
Embryo 6- 3% O226-May-9831.3204870809
Embryo 6- 3% O229-May-9863.6298573225
Embryo 6- 3% O22-Jun-98105.8460534778
Embryo 6- 3% O26-Jun-98147.9976958524
Embryo 6- 3% O29-Jun-98179.5730081831
Embryo 6- 3% O213-Jun-982111.3200374606
Embryo 6- 3% O216-Jun-982412.8394116197
Embryo 6- 3% O220-Jun-982814.5472308682
Embryo 6- 3% O226-Jun-983417.6649259109
Embryo 6- 3% O230-Jun-983820.4212375939
Embryo 6- 3% + UV23-May-9800
Embryo 6- 3% + UV26-May-9831.3204870809
Embryo 6- 3% + UV29-May-9863.6298573225
Embryo 6- 3% + UV2-Jun-98105.8460534778
Embryo 6- 3% + UV3-Jun-98116.1863308826
Embryo 6- 3% + UV9-Jun-98177.3263185999
Embryo 6- 3% + UV16-Jun-982410.4660063709
Embryo 6- 3% + UV20-Jun-982812.1350331364
Embryo 6- 3% + UV26-Jun-983414.9382601728
Embryo 6- 3% + UV30-Jun-983817.3738887669
Sheet3
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
Embryo 5-3% + UV
Embryo 5-3% O2
Embryo 6- 3% + UV
Embryo 6- 3% O2
days
Population doublings
Sheet2
Cell typeCumm cell#PassageDateDay# totalPD#totalCell typeCumm cell#PassageDateDay# totalPD#total
Embryo 5-20% O211910000123-May-9800Embryo 5-20% O211910000123-May-9800
Embryo 5-20% O227720000226-May-9831.2187538443Embryo 5-20% O227720000226-May-9831.2187538443Sum of PD#totalDay# totalSum of Cumm cell#Day# total
Embryo 5-20% O288703999.9999996329-May-9862.8968257494Embryo 5-20% O288703999.9999996329-May-9862.8968257494Cell type0361014172124283334384954Cell type0361014172124283334384954
Embryo 5-20% O2217029119.99999942-Jun-98104.1876433117Embryo 5-20% O2217029119.99999942-Jun-98104.1876433117Embryo 5-20% O201.21875384432.89682574944.18764331174.03028698385.15358147577.533017607110.84529553212.5112946048Embryo 5-20% O2119100002772000088703999.9999996217029119.999999194602777.599999423930214.3999972205850215.5946521911445474.906969532320307.0378
Embryo 5-20% O2194602777.59999956-Jun-98144.0302869838Embryo 5-20% O2194602777.59999956-Jun-98144.0302869838Embryo 5-3% O201.21875384433.33755652235.5318537427.55214850429.195235051411.2736171713.068552832815.078138881717.795495431920.1393769679Embryo 5-3% O21191000027720000120397200551017851.9999972235295752.946656981573735.036729485513074.305102314730367.838411987314281.1622709503236922.4413755244766109.6
Embryo 5-20% O2423930214.399997613-Jun-98215.1535814757Embryo 5-20% O2423930214.399997613-Jun-98215.1535814757Embryo 6- 3% O201.32048708093.62985732255.84605347787.99769585249.573008183111.320037460612.839411619714.547230868217.664925910920.4212375939Embryo 6- 3% O21202000030020000148799133.333333691419972.8888873072209412.869629155184050.3514430730901129.01388095249903.1704287777816350.3572497911445921.0916877555002940.2
Embryo 5-20% O22205850215.59465725-Jun-98337.5330176071Embryo 5-20% O22205850215.59465725-Jun-98337.5330176071Embryo 6-20% O201.32048708093.34078184314.96683153595.70379713017.0429345158.02860773359.0209082086Embryo 6-20% O21202000030020000121781133.333333375897764.888888626496274.8148131585035575.281483138746336.822216244079538.98765
Embryo 5-20% O221911445474.9069811-Jul-984910.845295532Embryo 5-20% O221911445474.9069811-Jul-984910.845295532
Embryo 5-20% O269532320307.0378916-Jul-985412.5112946048Embryo 5-20% O269532320307.0378916-Jul-985412.5112946048
Embryo 5-3% O211910000123-May-9800Embryo 5-3% O211910000123-May-9800
Embryo 5-3% O227720000226-May-9831.2187538443Embryo 5-3% O227720000226-May-9831.2187538443
Embryo 5-3% O2120397200329-May-9863.3375565223Embryo 5-3% O2120397200329-May-9863.3375565223
Embryo 5-3% O2551017851.99999742-Jun-98105.531853742Embryo 5-3% O2551017851.99999742-Jun-98105.531853742
Embryo 5-3% O22235295752.9466556-Jun-98147.5521485042Embryo 5-3% O22235295752.9466556-Jun-98147.5521485042
Embryo 5-3% O26981573735.036769-Jun-98179.1952350514Embryo 5-3% O26981573735.036769-Jun-98179.1952350514
Embryo 5-3% O229485513074.305713-Jun-982111.27361717Embryo 5-3% O229485513074.305713-Jun-982111.27361717
Embryo 5-3% O2102314730367.838816-Jun-982413.0685528328Embryo 5-3% O2102314730367.838816-Jun-982413.0685528328
Embryo 5-3% O2411987314281.162920-Jun-982815.0781388817Embryo 5-3% O2411987314281.162920-Jun-982815.0781388817
Embryo 5-3% O22709503236922.441026-Jun-983417.7954954319Embryo 5-3% O22709503236922.441026-Jun-983417.7954954319
Embryo 5-3% O213755244766109.61130-Jun-983820.1393769679Embryo 5-3% O213755244766109.61130-Jun-983820.1393769679
Embryo 5-3% + UV200000012-Jun-9805.531853742Embryo 6-20% O212020000123-May-9800
Embryo 5-3% + UV264000023-Jun-9815.9323916716Embryo 6-20% O230020000226-May-9831.3204870809
Embryo 5-3% + UV555000039-Jun-9877.0043415135Embryo 6-20% O2121781133.333333329-May-9863.3407818431
Embryo 5-3% + UV47767000.0000001416-Jun-981410.1097961083Embryo 6-20% O2375897764.88888842-Jun-98104.9668315359
Embryo 5-3% + UV169413626.666667520-Jun-981811.9362598533Embryo 6-20% O2626496274.81481356-Jun-98145.7037971301
Embryo 5-3% + UV1212436854.84444626-Jun-982414.7755476386Embryo 6-20% O21585035575.28148625-Jun-98337.042934515
Embryo 5-3% + UV5472131671.53123730-Jun-982816.9497409717Embryo 6-20% O23138746336.82221711-Jul-98498.0286077335
Embryo 6-20% O212020000123-May-9800Embryo 6-20% O26244079538.98765816-Jul-98549.0209082086
Embryo 6-20% O230020000226-May-9831.3204870809Embryo 6- 3% O212020000123-May-9800
Embryo 6-20% O2121781133.333333329-May-9863.3407818431Embryo 6- 3% O230020000226-May-9831.3204870809
Embryo 6-20% O2375897764.88888842-Jun-98104.9668315359Embryo 6- 3% O2148799133.333333329-May-9863.6298573225
Embryo 6-20% O2626496274.81481356-Jun-98145.7037971301Embryo 6- 3% O2691419972.88888742-Jun-98105.8460534778
Embryo 6-20% O21585035575.28148625-Jun-98337.042934515Embryo 6- 3% O23072209412.8696256-Jun-98147.9976958524
Embryo 6-20% O23138746336.82221711-Jul-98498.0286077335Embryo 6- 3% O29155184050.3514469-Jun-98179.5730081831
Embryo 6-20% O26244079538.98765816-Jul-98549.0209082086Embryo 6- 3% O230730901129.013713-Jun-982111.3200374606
Embryo 6- 3% O212020000123-May-9800Embryo 6- 3% O288095249903.1704816-Jun-982412.8394116197
Embryo 6- 3% O230020000226-May-9831.3204870809Embryo 6- 3% O2287777816350.357920-Jun-982814.5472308682
Embryo 6- 3% O2148799133.333333329-May-9863.6298573225Embryo 6- 3% O22497911445921.091026-Jun-983417.6649259109
Embryo 6- 3% O2691419972.88888742-Jun-98105.8460534778Embryo 6- 3% O216877555002940.21130-Jun-983820.4212375939
Embryo 6- 3% O23072209412.8696256-Jun-98147.9976958524
Embryo 6- 3% O29155184050.3514469-Jun-98179.5730081831
Embryo 6- 3% O230730901129.013713-Jun-982111.3200374606
Embryo 6- 3% O288095249903.1704816-Jun-982412.8394116197
Embryo 6- 3% O2287777816350.357920-Jun-982814.5472308682
Embryo 6- 3% O22497911445921.091026-Jun-983417.6649259109
Embryo 6- 3% O216877555002940.21130-Jun-983820.4212375939
Embryo 6- 3% + UV200000012-Jun-9805.8460534778
Embryo 6- 3% + UV253200023-Jun-9816.1863308826
Embryo 6- 3% + UV558000039-Jun-9877.3263185999
Embryo 6- 3% + UV49178400416-Jun-981410.4660063709
Embryo 6- 3% + UV156387312520-Jun-981812.1350331364
Embryo 6- 3% + UV1091583437.76626-Jun-982414.9382601728
Embryo 6- 3% + UV5905466398.28157730-Jun-982817.3738887669
Sheet2
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
Embryo 5-20% O2
Embryo 5-3% O2
Embryo 6- 3% O2
Embryo 6-20% O2
Days
Population doublings
Sheet1
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
Embryo 5-20% O2
Embryo 5-3% O2
Embryo 6- 3% O2
Embryo 6-20% O2
Days
Cummulative cell number
PDWorksheet
Embryo 5-20% O2DATA
Cumm cell#PassageDateDay# totalPD#total
11910000123-May-9800
27720000226-May-9831.2187538443
88703999.9999996329-May-9862.8968257494
217029119.99999942-Jun-98104.1876433117
194602777.59999956-Jun-98144.0302869838
423930214.399997613-Jun-98215.1535814757
2205850215.59465725-Jun-98337.5330176071
21911445474.9069811-Jul-984910.845295532
69532320307.0378916-Jul-985412.5112946048
Embryo 5-3% O2DATA
Cumm cell#PassageDateDay# totalPD#total
11910000123-May-9800
27720000226-May-9831.2187538443
120397200329-May-9863.3375565223
551017851.99999742-Jun-98105.531853742
2235295752.9466556-Jun-98147.5521485042
6981573735.036769-Jun-98179.1952350514
29485513074.305713-Jun-982111.27361717
102314730367.838816-Jun-982413.0685528328
411987314281.162920-Jun-982815.0781388817
2709503236922.441026-Jun-983417.7954954319
13755244766109.61130-Jun-983820.1393769679
Embryo 5-3% + UVDATA
Cumm cell#PassageDateDay# totalPD#total
200000012-Jun-9805.531853742
264000023-Jun-9815.9323916716
555000039-Jun-9877.0043415135
47767000.0000001416-Jun-981410.1097961083
169413626.666667520-Jun-981811.9362598533
1212436854.84444626-Jun-982414.7755476386
5472131671.53123730-Jun-982816.9497409717
Embryo 6-20% O2DATA
Cumm cell#PassageDateDay# totalPD#total
12020000123-May-9800
30020000226-May-9831.3204870809
121781133.333333329-May-9863.3407818431
375897764.88888842-Jun-98104.9668315359
626496274.81481356-Jun-98145.7037971301
1585035575.28148625-Jun-98337.042934515
3138746336.82221711-Jul-98498.0286077335
6244079538.98765816-Jul-98549.0209082086
Embryo 6- 3% O2DATA
Cumm cell#PassageDateDay# totalPD#total
12020000123-May-9800
30020000226-May-9831.3204870809
148799133.333333329-May-9863.6298573225
691419972.88888742-Jun-98105.8460534778
3072209412.8696256-Jun-98147.9976958524
9155184050.3514469-Jun-98179.5730081831
30730901129.013713-Jun-982111.3200374606
88095249903.1704816-Jun-982412.8394116197
287777816350.357920-Jun-982814.5472308682
2497911445921.091026-Jun-983417.6649259109
16877555002940.21130-Jun-983820.4212375939
Embryo 6- 3% + UVDATA
Cumm cell#PassageDateDay# totalPD#total
200000012-Jun-9805.8460534778
253200023-Jun-9816.1863308826
558000039-Jun-9877.3263185999
49178400416-Jun-981410.4660063709
156387312520-Jun-981812.1350331364
1091583437.76626-Jun-982414.9382601728
5905466398.28157730-Jun-982817.3738887669
isolations
Embryo 5-20% O2DATADATADATA
Cumm cell#PassageDateDay# totalDay#increm>Days/>PDCell# (out)PD#totalPD# incremCell# (in)# of T150 flasks# of pelletscell# per pelletLabel in the tubes
119100001 (thawed)23-May-9800000119100002 in 3% O2
27720000226-May-98332.4615306972277200001.21875384431.2187538443300000030Rest of cells frozen to repeat
88703999.9999998329-May-98631.787766061196000002.89682574941.678071905130000003166000005A-20%
217029119.99999942-Jun-981043.098811262573400004.18764331171.290817562330000003143400005B-20%
194602777.59999956-Jun-98144-25.420013623826900004.0302869838-0.1573563279269000030
423930214.399998613-Jun-982176.231669478258600005.15358147571.12329449199000003149600005C-20%(senesc)
2205850215.59466725-Jun-9833125.043211642146830007.53301760712.37943613156000003140830005D-20%
21911445474.9069811-Jul-9849164.8305125243596000010.8452955323.312277924915000003144600005E - 20%
69532320307.0379916-Jul-985453.0012021505476000012.51129460481.66599907281500003146100005F-20%
010-35938-359920000
011-3593800000
012-3593800000
013-3593800000
Embryo 5-3% O2DATADATADATA
Cumm cell#PassageDateDay# totalDay#increm>Days/>PDCell# (out)PD#totalPD# incremCell# (in)# of T150 flasks# of pelletscell# per pelletLabel in the tubes
119100001 (thawed)23-May-9800000119100002 in 3% O2
27720000226-May-98332.4615306972277200001.21875384431.2187538443300000030Rest of cells frozen to repeat
120397200329-May-98631.4158940004130300003.33755652232.1188026781300000031100300005A-3%
55101785242-Jun-981041.8229071086137300005.5318537422.194297219730000003 and UV (1)177300005B-3%
2235295752.9466756-Jun-981441.9799091077121700007.55214850422.020294762230000003191700005C-3%
6981573735.0367569-Jun-981731.825832002193700009.19523505141.643086547230000003163700005D-3%
29485513074.3052713-Jun-982141.92457390971267000011.273617172.078382118630000003196700005E - 3%
102314730367.839816-Jun-982431.67136909821041000013.06855283281.794935662830000003174100005F-3%
411987314281.164920-Jun-982841.99045967821208000015.07813888172.009586048830000003190800005G-3%
2709503236922.451026-Jun-983462.20802823961973000017.79549543192.7173565502300000031167300005H-3%
13755244766109.61130-Jun-983841.70657089051523000020.13937696792.3438815364000000Frozen1112300005I-3%
Embryo 5-3% + UVDATADATADATA
Cumm cell#PassageDateDay# totalDay#increm>Days/>PDCell# (out)PD#totalPD# incremCell# (in)# of T150 flasks# of pelletscell# per pelletLabel in the tubes
20000001 (see 3% O2)2-Jun-980005.531853742020000001
2640000UV before passage 23-Jun-98112.496642455426400005.93239167160.4005379296264000030
555000039-Jun-98765.59727681855500007.00434151351.071949841915000003140500005A-UV
47767000416-Jun-981472.25409832481291000010.10979610833.105454594830000003199100005B-UV
169413626.666667520-Jun-981842.19002430841064000011.93625985331.82646374530000003176400005C-UV
1212436854.84444626-Jun-982462.11320600572147000014.77554763862.8392877854300000031184700005D-UV
5472131671.53125730-Jun-982841.83976279351354000016.94974097172.17419333314000000Frozen195400005E - UV
Embryo 6-20% O2DATADATADATA
Cumm cell#PassageDateDay# totalDay#increm>Days/>PDCell# (out)PD#totalPD# incremCell# (in)# of T150 flasks# of pelletscell# per pelletLabel in the tubes
120200001 (thawed)23-May-9800000120200002 in 3% O2
30020000226-May-98332.2718889441300200001.32048708091.3204870809300000030Rest of cells frozen to repeat
121781133.333334329-May-98631.4849318308121700003.34078184312.020294762230000003191700006A-20%
375897764.88888942-Jun-981042.459949420992600004.96683153591.626049692830000003162600006B-20%
626496274.81481656-Jun-981445.427661795450000005.70379713010.73696559423000003147000006C-20%(senesc)
1585035575.28148625-Jun-98331914.18823805087590007.0429345151.339137384975900030
3138746336.82223711-Jul-98491616.232560345215030008.02860773350.9856732185150300030
6244079538.98766816-Jul-985455.038796337529900009.02090820860.99230047521500003128400006D-20%
09-35938-359920000
010-3593800000
011-3593800000
012-3593800000
013-3593800000
Embryo 6- 3% O2DATADATADATA
Cumm cell#PassageDateDay# totalDay#increm>Days/>PDCell# (out)PD#totalPD# incremCell# (in)# of T150 flasks# of pelletscell# per pelletLabel in the tubes
120200001 (thawed)23-May-9800000120200002 in 3% O2
30020000226-May-98332.2718889441300200001.32048708091.3204870809300000030Rest of cells frozen to repeat
148799133.333334329-May-98631.2990554507148700003.62985732252.3093702416300000031118700006A-3%
691419972.8888942-Jun-981041.8048943864139400005.84605347782.216196155430000003 and UV (1)179400006B-3%
3072209412.8696356-Jun-981441.8590450008133300007.99769585242.1516423746300000031103300006C-3%
9155184050.3515169-Jun-981731.904384255589400009.57300818311.575312330730000003159400006D-3%
30730901129.0132713-Jun-982142.2896010111007000011.32003746061.747029277530000003170700006E - 3%
88095249903.1712816-Jun-982431.9744971849860000012.83941161971.519374159130000003156000006F-3%
287777816350.359920-Jun-982842.3421682379980000014.54723086821.707819248530000003168000006G-3%
2497911445921.121026-Jun-983461.92449868182604000017.66492591093.1176950427300000031230400006H-3%
16877555002940.31130-Jun-983841.45121468842027000020.42123759392.7563116834000000Frozen1162700006I-3%
Embryo 6- 3% + UVDATADATADATA
Cumm cell#PassageDateDay# totalDay#increm>Days/>PDCell# (out)PD#totalPD# incremCell# (in)# of T150 flasks# of pelletscell# per pelletLabel in the tubes
20000001 (see 3% O2)2-Jun-980005.8460534778020000001
2532000UV before passage 23-Jun-98112.938778731725320006.18633088260.3402774048253200030
558000039-Jun-98765.263214602255800007.32631859991.139987717315000003140800006A-UV
49178400416-Jun-981472.22952105771322000010.46600637093.139687771300000031102200006B-UV
156387312520-Jun-981842.3966062634954000012.13503313641.669026765530000003165400006C-UV
1091583437.76626-Jun-982462.14039031522094000014.93826017282.8032270364300000031179400006D-UV
5905466398.28159730-Jun-982841.64228651681623000017.37388876692.43562859414000000Frozen1122300006E - UV
&F
Page &P
rescues6
MEF-Embryo 6
IDsamplecells(x106)lysis volumeTEOD260OD280260/280conc(ug/ul)DNA (ug)new TEnew concentugDNA/106 cellsdate isolated
0NT600ul07/22/02
16A-3%600ul1000.5590.3021.852.80279.52.807/22/02
26B-3%600ul1000.3060.1681.821.531531.537/22/02
36C-3%600ul1000.4510.241.882.26225.52.267/22/02
46D-3%600ul1000.4340.2341.852.172172.177/22/02
56E-3%600ul1000.2260.1261.791.131131.137/22/02
66F-3%600ul1000.320.1691.891.601601.607/22/02
76G-3%600ul1000.3380.1791.891.691691.697/22/02
86H-3%600ul1000.7080.3821.853.543543001.187/22/02
96I-3%600ul1000.8650.4521.914.33432.53001.447/22/02
106A-20%600ul1000.5460.2911.882.732732001.377/22/02
116B-20%600ul1000.4660.2541.832.332332001.177/22/02
126C-20%600ul1000.2210.1211.831.11110.51.117/22/02
136D-20%600ul1000.2510.1331.891.26125.51.267/22/02
146A-3%UV600ul1000.2020.1081.871.011011.017/22/02
156B-3%UV600ul1001.0080.681.485.045043001.687/22/02
166C-3%UV600ul1000.3750.2051.831.88187.51.887/22/02
176D-3%UV600ul1000.6070.3321.833.04303.52001.527/22/02
186E-3%UV600ul1000.3490.1861.881.75174.51.757/22/02
MEF-Embryo 5
IDsamplecells(x106)lysis volumeTEOD260OD280260/280conc(ug/ul)DNA (ug)new TEnew concentugDNA/106 cellsdate isolated
0NT600ul0.3080.14908/16/02
15A-3%600ul1000.5230.2332.242.62261.58/16/02
25B-3%600ul1000.4010.1842.182.01200.58/16/02
35C-3%600ul1000.3940.1822.161.971978/16/02
45D-3%600ul1000.3470.1552.241.74173.58/16/02
55E-3%600ul1000.480.002.402408/16/02
65F-3%600ul1000.3470.162.171.74173.58/16/02
75G-3%600ul1000.4240.1892.242.122128/16/02
85H-3%600ul1000.6440.2892.233.223228/16/02
95I-3%600ul1000.440.1992.212.202208/16/02
105A-20%600ul1000.3430.1552.211.72171.58/16/02
115B-20%600ul1000.2440.112.221.221228/16/02
125C-20%600ul1000.2920.1412.071.461468/16/02
135D-20%600ul1000.2790.1252.231.40139.58/16/02
145E-20%600ul1000.310.142.211.551558/16/02
155F-20%600ul1000.2920.1342.181.46146
165A-3%UV600ul1000.2430.1122.171.22121.58/16/02
175B-3%UV600ul1000.520.2382.182.602608/16/02
185C-3%UV600ul1000.4220.1912.212.112118/16/02
195D-3%UV600ul1000.5770.2582.242.89288.58/16/02
205E-3%UV600ul1000.4960.2312.152.482488/16/02
MEF-Embryo 6
IDsamplecells(x106)lysis volumeTEOD260OD280260/280conc(ug/ul)DNA (ug)new TEnew concentugDNA/106 cellsdate isolated
0NT600ul0.2630.121.3208/26/02
16E-20%600ul1000.2750.1282.151.38137.58/26/02
26F-20%600ul2000.5010.2282.202.515018/26/02
35G-20%600ul1000.3630.1612.251.82181.58/26/02
45H-20%600ul1000.7340.3332.203.673678/26/02
5Hum clone12F600ul2000.3440.1622.121.723448/26/02
AVERAGES6
Rescue 7-25-02Rescue 7-29-02Rescue 7-29-02Rescue 8-12-02
IDsampleconc(ug/ul)ug DNADNA ulH2OTCcellsX-galP-galMF(x10-5)Day# TotalPD# TotalHarvestnotesIDsampleconc(ug/ul)ug DNADNA ulH2OTCcellsX-galP-galMF(x10-5)Day# TotalPD# TotalnotesMFdaysPDIDsampleconc(ug/ul)ug DNADNA ulH2OTCcellsX-galP-galMF(x10-5)Day# TotalPD# TotalnotesMFdaysPDIDsampleconc(ug/ul)ug DNADNA ulH2OTCcellsX-galP-galMF(x10-5)Day# TotalPD# Total
0NT004.57/12/020100NT004.57/12/02000NT004.57/12/020100NT004.57/12/02000
16A-3%2.80207.1650.844.57/12/02450398.6763.62985732258.673.6298573225616A-3%2.80207.1650.844.57/12/02216146.4863.62985732256.4863.629857322516A-3%2.80207.1650.844.57/12/021470.0063.62985732256.4863.629857322516A-3%2.80207.1650.844.57/12/02150128.0063.6298573225
26B-3%1.532013.0744.934.57/12/023463710.69105.846053477810.695.84605347781026B-3%1.532013.0744.934.57/12/02157138.28105.84605347788.28105.846053477826B-3%1.532013.0744.934.57/12/021531711.11105.84605347788.28105.846053477826D-3%2.17209.2248.784.57/12/021302317.69179.5730081831
36C-3%2.26208.8749.134.57/12/023465415.61147.997695852415.617.99769585241436C-3%2.26208.8749.134.57/12/022213114.03147.997695852414.03147.997695852436C-3%2.26208.8749.134.57/12/021281814.06147.997695852414.03147.997695852436E-3%1.132017.7040.304.57/12/026327311.552111.3200374606
46D-3%2.17209.2248.784.57/12/02672943.28179.573008183119.166666666711.32003746062146D-3%2.17209.2248.784.57/12/021642716.46179.573008183116.46179.573008183146D-3%2.17209.2248.784.57/12/02811619.75179.573008183116.46179.573008183146G-3%1.692011.8346.174.57/12/021452114.482814.5472308682
56E-3%1.132017.7040.304.57/12/023606919.172111.320037460618.965517241412.83941161972456E-3%1.132017.7040.304.57/12/02266228.272111.32003746068.272111.320037460656E-3%1.132017.7040.304.57/12/022433213.172111.32003746068.272111.320037460656H-3%1.182016.9541.054.57/12/023483911.213417.6649259109
66F-3%1.602012.5045.504.57/12/022324418.972412.839411619710.112359550614.54723086822866F-3%1.602012.5045.504.57/12/021332518.802412.839411619718.802412.839411619766F-3%1.602012.5045.504.57/12/02761418.422412.839411619718.802412.839411619766A-20%1.372014.6543.354.57/12/02218146.4263.3407818431
76G-3%1.692011.8346.174.57/12/021781810.112814.547230868214.095744680917.66492591093476G-3%1.692011.8346.174.57/12/02431330.232814.54723086829.523417.664925910976G-3%1.692011.8346.174.57/12/02751114.672814.54723086829.523417.664925910976B-20%1.172017.1740.834.57/12/0212197.44104.9668315359
86H-3%1.182016.9541.054.57/12/023765314.103417.664925910911.006289308220.42123759393886H-3%1.182016.9541.054.57/12/02210209.523417.664925910910.233820.421237593986H-3%1.182016.9541.054.57/12/021701810.593417.664925910910.233820.421237593986C-20%1.112018.1039.904.57/12/022033014.78145.7037971301
96I-3%1.442013.8744.134.57/12/023183511.013820.421237593996I-3%1.442013.8744.134.57/12/022152210.233820.421237593996I-3%1.442013.8744.134.57/12/02149149.403820.421237593996D-20%1.262015.9442.064.57/12/021022827.45549.0209082086
106A-20%1.372014.6543.354.57/12/021832212.0263.3407818431106A-20%1.372014.6543.354.57/12/0210798.4163.34078184318.411214953363.3407818431106A-20%1.372014.6543.354.57/12/02150138.6763.34078184318.411214953363.3407818431106A-3%UV1.012019.8038.204.57/12/02152213140.1377.3263185999
116B-20%1.172017.1740.834.57/12/022963311.15104.9668315359116B-20%1.172017.1740.834.57/12/021151815.65104.966831535915.652173913104.9668315359116B-20%1.172017.1740.834.57/12/0223521.74104.966831535915.652173913104.9668315359116E-3%UV1.752011.4646.544.57/12/02298353118.462817.3738887669
126C-20%1.112018.1039.904.57/12/023415114.96145.7037971301senescent126C-20%1.112018.1039.904.47/12/029444.44145.7037971301senescent15.3333333333549.0209082086126C-20%1.112018.1039.904.47/12/02902022.22145.7037971301senescent15.3333333333549.020908208612#30 ctrl1.962010.204081632747.79591836734.57/12/02720385.28
136D-20%1.262015.9442.064.57/12/021804625.56549.0209082086136D-20%1.262015.9442.064.57/12/021502315.33549.0209082086136D-20%1.262015.9442.064.57/12/02872528.74549.0209082086
146A-3%UV1.012019.8038.204.57/12/02247388157.0977.3263185999146A-3%UV1.012019.8038.204.57/12/02146198135.6277.3263185999146A-3%UV1.012019.8038.204.57/12/02701825.7177.3263185999
156B-3%UV1.682011.9046.104.57/12/02320373116.561410.4660063709156B-3%UV1.682011.9046.104.57/12/02133134100.751410.4660063709156B-3%UV1.682011.9046.104.57/12/02168173102.981410.4660063709
166C-3%UV1.882010.6747.334.57/12/0240640499.511812.1350331364166C-3%UV1.882010.6747.334.57/12/0212411794.351812.1350331364166C-3%UV1.882010.6747.334.57/12/021049389.421812.1350331364
176D-3%UV1.522013.1844.824.57/12/0224422692.622414.9382601728176D-3%UV1.522013.1844.824.57/12/0230625884.312414.9382601728176D-3%UV1.522013.1844.824.57/12/02390391100.262414.9382601728
186E-3%UV1.752011.4646.544.57/12/02380428112.632817.3738887669186E-3%UV1.752011.4646.544.57/12/02231303131.172817.3738887669186E-3%UV1.752011.4646.544.57/12/02288490170.142817.3738887669
19#30 ctrl1.962010.2047.804.57/12/02632507.9119#30 ctrl1.962010.204081632747.79591836734.57/12/022532610.2819#30 ctrl1.962010.2047.804.57/12/02261186.90
206D-3%2.17209.2248.784.57/12/02421126.19
216E-3%1.132017.7040.304.57/12/02111109.01
226G-3%1.692011.8346.174.57/12/02321443.75
236H-3%1.182016.9541.054.57/12/021332216.54
246C-20%1.112018.1039.904.47/12/02361130.56
256D-20%1.262015.9442.064.57/12/02491428.57
RESCUE 8-29-02
IDsampleconc(ug/ul)ug DNADNA ulH2OTCcellsX-galP-galMF(x10-5)
0NT1.322015.242.84.58/27/02000
16A-3%2.80207.250.84.58/27/022312510.82
26A-3%2.80207.250.84.58/27/021692615.38
36I-3%1.442013.944.14.58/27/021813217.68
46I-3%1.442013.944.14.58/27/021392417.27
56A-20%1.372014.743.34.58/27/02141117.80ctrl
66A-20%1.372014.743.34.58/27/02220198.64ctrl
76E-20%1.382014.543.54.58/27/021584830.38
86F-20%2.51208.050.04.58/27/021444631.94
9#30 ctrl1.962010.247.84.58/27/0233233611.15
1012F1.722011.646.44.58/27/02000.00
AVERAGES6
00
00
00
00
0
0
0
0
0
6-3%O2
6-20%O2
PD#Total
mutation frequency (x10-5)
MEF 6
RESCUES 5
00
00
00
00
0
0
0
0
6-3%02
6-20%
PD#Total
Mutation Frequency (x10-5)
MEF6* #1
Averages5
00
00
00
00
00
0
0
0
6-3%O2
6-3%02UV
PD#Total
Mutation Frequency (x10-5)
MEF6-UV#1
combined growth curves
00
00
00
00
0
0
0
0
6-3%02
6-20%
Day#Total
Mutation Frequency (x10-5)
MEF6* #1
00
00
00
00
00
0
0
0
6-3%O2
6-3%02UV
Day#Total
Mutation Frequency (x10-5)
MEF6-UV#1
00
00
00
0
0
0
0
0
6-3%02
6-20%
PD#Total
Mutation Frequency (x10-5)
MEF6* #2
00
00
00
00
00
0
0
0
6-3%O2
6-3%02UV
PD#Total
Mutation Frequency (x10-5)
MEF6-UV#2
00
00
00
0
0
0
0
0
6-3%02
6-20%
Days
Mutation Frequency (x10-5)
MEF6* #2
00
00
00
00
00
0
0
0
6-3%O2
6-3%02UV
Days
Mutation Frequency (x10-5)
MEF6-UV#2
Averages
IDsample7/24/987/28/988/5/028/5/028/12/028/29/029/23/029/23/02AveragestdevPD
0NT00
16A-3%8.676.4812.248.0010.829.22.33.6
26B-3%10.698.2811.119.71.55.8
36C-3%15.6114.0314.0614.00.98.0
46D-3%16.4619.7517.6918.01.79.6
56E-3%19.178.2713.179.0111.5510.54.311.3
66F-3%18.9718.8018.4218.60.312.8
76G-3%10.1114.6714.4814.62.614.5
86H-3%14.109.5210.5916.5411.2112.02.917.7
96I-3%11.0110.239.4017.6817.2713.14.020.4
106A-20%12.028.418.676.427.808.648.71.83.340782
116B-20%11.1515.657.4411.54.14.966832
126C-20%14.9622.2214.7818.54.25.703797
136D-20%25.5615.3328.7428.5727.4525.05.69.0209082
6E-20%30.3840.0031.1133.85.413.8868270
6F-20%31.9434.8333.6033.51.416.9537773
146A-3%UV157.09135.62140.13137.911.3
156B-3%UV116.56100.75102.98101.98.6
166C-3%UV99.5194.3589.4291.95.0
176D-3%UV92.6284.31100.2692.38.0
186E-3%UV112.63131.17118.46124.89.5
19#30 ctrl7.9110.286.905.287.52.1
Cumulative7/24/987/28/98SUMMF(x10-5)3%O2
IDsampleX-galP-galX-galP-galX-galP-galX-galP-gal20%O2
0NT010010
16A-3%4503921614666537.96
26B-3%3463715713503509.94
36C-3%34654221315678514.99
46D-3%6729164272315624.24
56E-3%36069266226269114.54
66F-3%23244133253656918.90
76G-3%178181781810.11
86H-3%37653210205867312.46
96I-3%31835215225335710.69
106A-20%1832210792903110.69
116B-20%29633115184115112.41
126C-20%341513415114.96
136D-20%18046150233306920.91
146A-3%UV247388146198393586149.11
156B-3%UV320373133134453507111.92
166C-3%UV40640412411753052198.30
176D-3%UV24422630625855048488.00
186E-3%UV380428231303611731119.64
19#30 ctrl6325025326885768.59
002.29180274962.29180274961.84988161521.8498816152
001.52820506961.52820506964.11345175544.1134517554
000.90205882840.90205882844.24737156894.2473715689
001.66227392291.66227392295.62109280885.6210928088
004.34485506164.34485506165.35562804185.3556280418
000.27873914830.27873914831.44869034531.4486903453
02.57798123572.5779812357
02.87247411682.8724741168
04.02038027144.0203802714
3%O2
20%O2
PD
Mutation Frequency (x10-5)
Average MF for MEF 6 (PD)
002.29180274962.29180274961.84988161521.8498816152
001.52820506961.52820506964.11345175544.1134517554
000.90205882840.90205882844.24737156894.2473715689
001.66227392291.66227392295.62109280885.6210928088
04.34485506164.3448550616
00.27873914830.2787391483
02.57798123572.5779812357
02.87247411682.8724741168
04.02038027144.0203802714
MEF6-3%O2
MEF6-20%O2
Days Total
Mutation Frequency (x10-5)
Average MF for MEF6 (vs Days)
002.29180274962.291802749611.318905089511.3189050895
001.52820506961.52820506968.55872925938.5587292593
000.90205882840.90205882845.04255893765.0425589376
001.66227392291.66227392297.97372901167.9737290116
004.34485506164.34485506169.47949101369.4794910136
00.27873914830.2787391483
02.57798123572.5779812357
02.87247411682.8724741168
04.02038027144.0203802714
MEF6-3%O2
MEF6-3%O2UV
PD
Mutation Frequency (x10-5)
Average for MEF6-UV
002.29180274962.291802749611.318905089511.3189050895
001.52820506961.52820506968.55872925938.5587292593
000.90205882840.90205882845.04255893765.0425589376
001.66227392291.66227392297.97372901167.9737290116
004.34485506164.34485506169.47949101369.4794910136
00.27873914830.2787391483
02.57798123572.5779812357
02.87247411682.8724741168
04.02038027144.0203802714
MEF6-3%O2
MEF6-3%O2UV
Days
Mutation Frequency (x10-5)
Average for MEF6-UV
Rescue 9-3-02Rescue 9-12-02Rescue 9-17-02Rescue 9-23-02
IDsampleconc(ug/ul)ug DNADNA ulH2OTCcellsX-galP-galMF(x10-5)IDsampleconc(ug/ul)ug DNADNA ulH2OTCcellsX-galP-galMF(x10-5)IDsampleconc(ug/ul)ug DNADNA ulH2OTCcellsX-galP-galMF(x10-5)IDsampleconc(ug/ul)ug DNADNA ulH2OTCcellsX-galP-galMF(x10-5)
0NT1.322015.1542.854.57/12/020100NT1.322015.1542.854.59/19/010200NT1.322015.1542.854.59/19/010100NT1.322015.1542.854.59/19/01000
15A-3%2.62207.6550.354.57/12/0210298.8215A-3%2.62207.6550.354.59/19/013083812.3415A-3%2.62207.6550.354.59/19/012352611.0615A-3%2.62207.6550.354.59/19/01313268.31
25B-3%2.01209.9848.024.57/12/02741013.5125B-3%2.01209.9848.024.59/19/01133107.5225B-3%2.01209.9848.024.59/19/01266145.2625B-3%2.01209.9848.024.59/19/0114296.34
35C-3%1.972010.1547.853.97/12/029499.5735C-3%1.972010.1547.854.59/19/011542616.8835C-3%1.972010.1547.854.59/19/012883311.4635C-3%1.972010.1547.854.59/19/01358236.42
45D-3%1.742011.5346.474.57/12/028344.8245D-3%1.742011.5346.474.59/19/01139117.9145D-3%1.742011.5346.474.09/19/01350349.7145D-3%1.742011.5346.474.59/19/013294714.29
55E-3%2.40208.3349.674.57/12/026669.0955E-3%2.40208.3349.674.59/19/011741810.3455E-3%2.40208.3349.674.59/19/013043411.1855E-3%2.40208.3349.674.59/19/012553011.76
65F-3%1.742011.5346.474.57/12/021061917.9265F-3%1.742011.5346.474.59/19/012402410.0065F-3%1.742011.5346.474.59/19/01376359.3165F-3%1.742011.5346.474.59/19/01324288.64
75G-3%2.12209.4348.574.57/12/029244.3575G-3%2.12209.4348.574.59/19/01160159.3875G-3%2.12209.4348.574.59/19/013203611.2575G-3%2.12209.4348.574.59/19/01402379.20
85H-3%3.22206.2151.794.47/12/028256.1085H-3%3.22206.2151.794.09/19/01219219.5985H-3%3.22206.2151.794.59/19/01306206.5485H-3%3.22206.2151.794.59/19/01431337.66
95I-3%2.20209.0948.914.57/12/02701217.1495I-3%2.20209.0948.914.59/19/01234114.7095I-3%2.20209.0948.914.39/19/0112564.8095I-3%2.20209.0948.914.59/19/01367359.54
105A-20%2.21209.0448.964.57/12/025347.55105A-20%2.21209.0448.964.59/19/01186158.06105A-20%2.21209.0448.964.59/19/01113108.85105A-20%2.21209.0448.964.59/19/01147106.80
115B-20%2.22209.0248.984.57/12/0218211.11115B-20%2.22209.0248.984.59/19/016369.52115B-20%2.22209.0248.984.59/19/0112597.20115B-20%2.22209.0248.984.59/19/012292711.79
125C-20%2.07209.6648.344.57/12/026369.52125C-20%2.07209.6648.344.59/19/012063617.48125C-20%2.07209.6648.344.59/19/011612314.29125C-20%2.07209.6648.344.59/19/01189168.47
135D-20%2.23208.9649.044.57/12/0238513.16135D-20%2.23208.9649.044.59/19/011271814.17135D-20%2.23208.9649.044.59/19/011391510.79135D-20%2.23208.9649.044.59/19/011542516.23
145E-20%2.21209.0348.974.57/12/0237718.92145E-20%2.21209.0348.974.59/19/011292620.16145E-20%2.21209.0348.974.59/19/01134139.70145E-20%2.21209.0348.974.59/19/011861910.22
155F-20%2.18209.1848.824.57/12/02841214.29155F-20%2.18209.1848.824.59/19/011071816.82155F-20%2.18209.1848.824.59/19/011262620.63155F-20%2.18209.1848.824.59/19/011482818.92
15a5G-20%1.822010.9947.014.57/12/022063717.9615a5G-20%1.822010.9947.014.59/19/011514026.4915a5G-20%1.822010.9947.014.59/19/0141010325.1215a5G-20%1.822010.9947.014.59/19/0129712241.08
15b5H-20%3.67205.4552.554.57/12/021815630.9415b5H-20%3.67205.4552.554.59/19/0165218027.6115b5H-20%3.67205.4552.554.59/19/0172018525.6915b5H-20%3.67205.4552.554.59/19/0140213333.08
165A-3%UV2.17209.2248.784.57/12/021538253.33165A-3%UV2.17209.2248.784.59/19/01140146104.29165A-3%UV2.17209.2248.784.59/19/01168208123.81165A-3%UV2.17209.2248.784.59/19/01172192111.63
175B-3%UV2.18209.1548.854.57/12/028891103.41175B-3%UV2.18209.1548.854.59/19/01196208106.12175B-3%UV2.18209.1548.854.59/19/01243280115.23175B-3%UV2.18209.1548.854.59/19/01315354112.38
185C-3%UV2.21209.0548.954.57/12/021127466.07185C-3%UV2.21209.0548.954.49/19/0183111133.73185C-3%UV2.21209.0548.954.49/19/01244266109.02185C-3%UV2.21209.0548.954.49/19/01321341106.23
195D-3%UV2.24208.9449.064.57/12/0225424797.24195D-3%UV2.24208.9449.064.59/19/0129728595.96195D-3%UV2.24208.9449.064.59/19/0147645896.22195D-3%UV2.24208.9449.064.59/19/0167666998.96
205E-3%UV2.15209.3148.694.57/12/0214610269.86205E-3%UV2.15209.3148.694.59/19/0152237772.22205E-3%UV2.15209.3148.694.59/19/0127323786.81205E-3%UV2.15209.3148.694.59/19/0141235084.95
21#30 ctrl1.962010.2047.804.57/12/02341288.2121#30 ctrl1.962010.2047.804.59/19/01736658.8321#30 ctrl1.962010.2047.804.59/19/01952626.5121#30 ctrl1.962010.2047.804.59/19/01632497.75
IDsample9/12/029/17/029/23/02AveragesstdevPD total
0NT00
15A-3%12.3411.068.3110.572.13.3375565223
25B-3%7.525.266.346.371.15.531853742
35C-3%16.8811.466.4211.595.27.5521485042
45D-3%7.919.7114.2910.643.39.1952350514
55E-3%10.3411.1811.7611.100.711.27361717
65F-3%10.009.318.649.320.713.0685528328
75G-3%9.3811.259.209.941.115.0781388817
85H-3%9.596.547.667.931.517.7954954319
95I-3%4.704.809.546.352.820.1393769679
105A-20%8.068.856.807.911.02.8968257494
115B-20%9.527.2011.799.502.34.1876433117
125C-20%17.4814.298.4713.414.65.1535814757
135D-20%14.1710.7916.2313.732.77.5330176071
145E-20%20.169.7010.2213.365.910.845295532
155F-20%16.8220.6318.9218.791.912.5112946048
15a5G-20%26.4925.1241.0830.908.818.5022494652
15b5H-20%27.6125.6933.0828.803.822.2012087368
165A-3%UV104.29123.81111.63113.249.97.0043415135
175B-3%UV106.12115.23112.38111.244.710.1097961083
185C-3%UV109.02106.23107.622.011.9362598533
195D-3%UV95.9696.2298.9697.051.714.7755476386
205E-3%UV72.2286.8184.9581.337.916.9497409717
21#30 ctrl8.836.517.757.701.2
002.06045891382.06045891381.03263062931.0326306293
001.12823378641.12823378642.29525595642.2952559564
005.23048614315.23048614314.56858980924.5685898092
003.28489319463.28489319462.74780256512.7478025651
000.71386190940.71386190945.89270276815.8927027681
000.6790505560.6790505561.90940713411.9094071341
001.13512629541.13512629548.84346180678.8434618067
001.54442810371.54442810373.83564857893.8356485789
02.76384933352.7638493335
MEF5-3%
MEF5-20%
PD Total
MF
009.86136164119.86136164112.06045891382.0604589138
004.65735737414.65735737411.12823378641.1282337864
001.96990321811.96990321815.23048614315.2304861431
001.6651838871.6651838873.28489319463.2848931946
007.94140718687.94140718680.71386190940.7138619094
00.6790505560.679050556
01.13512629541.1351262954
01.54442810371.5444281037
02.76384933352.7638493335
MEF5-3%
MEF5-3%UV
PD Total
MF
002.06045891382.06045891381.03263062931.0326306293
001.12823378641.12823378642.29525595642.2952559564
005.23048614315.23048614314.56858980924.5685898092
003.28489319463.28489319462.74780256512.7478025651
000.71386190940.71386190945.89270276815.8927027681
000.6790505560.6790505561.90940713411.9094071341
001.13512629541.13512629548.84346180678.8434618067
001.54442810371.54442810373.83564857893.8356485789
02.76384933352.7638493335
3%
20%
Population doublings total
Mutant frequency (x10-5)
00000000000000
3.33504167182.92700552112.69209835083.04073852143.10772421063.16581323783.6112035853.28066858832.84172474942.61967023531.21875384431.21875384431.32048708091.3204870809
6.29481182714.63040158256.1026203697.10193867786.50643940467.04617225074.79484889045.46494888274.68168433694.67538649882.89682574943.33755652233.34078184313.6298573225
8.59249237575.86367612159.416833498710.41421660268.98325310149.75986806556.43698921258.08475313276.72782611856.73428018794.18764331175.5318537424.96683153595.8460534778
11.12356186848.54974166512.536742962514.12864530810.670313789812.6330250468.473785568510.91730563488.47126927689.24186342874.03028698387.55214850425.70379713017.9976958524
13.291347959110.804738751715.834423511117.418479773215.59473043815.55268293778.756719531813.1712949019.783547201611.53758645325.15358147579.19523505147.0429345159.5730081831
15.679078112410.875128079620.813229288820.587335715720.991620590518.22774609297.533017607111.273617178.028607733511.3200374606
20.31521933712.87855898525.888679478423.097288529810.84529553213.06855283289.020908208612.8394116197
25.578253742915.954661964112.511294604815.078138881714.5472308682
19.320167877617.795495431917.6649259109
23.052075181920.139376967920.4212375939
28.3954830042
1-20%
1-3%
3-20%
3-3%
4-20%
4-3%
1-20% B
1-3% B
2-20%
2-3%
5-20%
5-3%
6-20%
6-3%
Days
PD
Chart8
9.2431113868.6599233282.29180274962.29180274961.84988161521.8498816152
9.695682944111.5450952211.52820506961.52820506964.11345175544.1134517554
14.044824660618.50027367270.90205882840.90205882844.24737156894.2473715689
17.969602915425.02284362021.66227392291.66227392295.62109280885.6210928088
10.49976072333.83028598224.34485506164.34485506165.35562804185.3556280418
18.609022556433.45863503950.27873914830.27873914831.44869034531.4486903453
14.57471264372.57798123572.5779812357
11.96507368832.87247411682.8724741168
13.11611313274.02038027144.0203802714
3%O2
20%O2
Population Doublings
Mutant Frequency (x10-5)
Sheet3
Cell typeDateDay# totalPD#total
Embryo 5-3% O223-May-9800
Embryo 5-3% O226-May-9831.2187538443
Embryo 5-3% O229-May-9863.3375565223
Embryo 5-3% O22-Jun-98105.531853742
Embryo 5-3% O26-Jun-98147.5521485042Sum of PD#totalDay# total
Embryo 5-3% O29-Jun-98179.1952350514Cell type036101114172124283438
Embryo 5-3% O213-Jun-982111.27361717Embryo 5-3% + UV01.21875384433.33755652235.5318537425.93239167167.004341513510.109796108311.936259853314.775547638616.9497409717
Embryo 5-3% O216-Jun-982413.0685528328Embryo 5-3% O201.21875384433.33755652235.5318537427.55214850429.195235051411.2736171713.068552832815.078138881717.795495431920.1393769679
Embryo 5-3% O220-Jun-982815.0781388817Embryo 6- 3% + UV01.32048708093.62985732255.84605347786.18633088267.326318599910.466006370912.135033136414.938260172817.3738887669
Embryo 5-3% O226-Jun-983417.7954954319Embryo 6- 3% O201.32048708093.62985732255.84605347787.99769585249.573008183111.320037460612.839411619714.547230868217.664925910920.4212375939
Embryo 5-3% O230-Jun-983820.1393769679
Embryo 5-3% + UV23-May-9800
Embryo 5-3% + UV26-May-9831.2187538443
Embryo 5-3% + UV29-May-9863.3375565223
Embryo 5-3% + UV2-Jun-98105.531853742
Embryo 5-3% + UV3-Jun-98115.9323916716
Embryo 5-3% + UV9-Jun-98177.0043415135
Embryo 5-3% + UV16-Jun-982410.1097961083
Embryo 5-3% + UV20-Jun-982811.9362598533
Embryo 5-3% + UV26-Jun-983414.7755476386
Embryo 5-3% + UV30-Jun-983816.9497409717
Embryo 6- 3% O223-May-9800
Embryo 6- 3% O226-May-9831.3204870809
Embryo 6- 3% O229-May-9863.6298573225
Embryo 6- 3% O22-Jun-98105.8460534778
Embryo 6- 3% O26-Jun-98147.9976958524
Embryo 6- 3% O29-Jun-98179.5730081831
Embryo 6- 3% O213-Jun-982111.3200374606
Embryo 6- 3% O216-Jun-982412.8394116197
Embryo 6- 3% O220-Jun-982814.5472308682
Embryo 6- 3% O226-Jun-983417.6649259109
Embryo 6- 3% O230-Jun-983820.4212375939
Embryo 6- 3% + UV23-May-9800
Embryo 6- 3% + UV26-May-9831.3204870809
Embryo 6- 3% + UV29-May-9863.6298573225
Embryo 6- 3% + UV2-Jun-98105.8460534778
Embryo 6- 3% + UV3-Jun-98116.1863308826
Embryo 6- 3% + UV9-Jun-98177.3263185999
Embryo 6- 3% + UV16-Jun-982410.4660063709
Embryo 6- 3% + UV20-Jun-982812.1350331364
Embryo 6- 3% + UV26-Jun-983414.9382601728
Embryo 6- 3% + UV30-Jun-983817.3738887669
Sheet3
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
Embryo 5-3% + UV
Embryo 5-3% O2
Embryo 6- 3% + UV
Embryo 6- 3% O2
days
Population doublings
Sheet2
Cell typeCumm cell#PassageDateDay# totalPD#totalCell typeCumm cell#PassageDateDay# totalPD#totalSum of PD#totalDay# total
Embryo 5-20% O211910000123-May-9800Embryo 5-20% O211910000123-May-9800Cell type03610141721242833343849547784
Embryo 5-20% O227720000226-May-9831.2187538443Embryo 5-20% O227720000226-May-9831.2187538443Embryo 5-20% O201.21875384432.89682574944.18764331174.03028698385.15358147577.533017607110.84529553212.511294604818.502249465222.2012087368
Embryo 5-20% O288703999.9999996329-May-9862.8968257494Embryo 5-20% O288703999.9999996329-May-9862.8968257494Embryo 5-3% O201.21875384433.33755652235.5318537427.55214850429.195235051411.2736171713.068552832815.078138881717.795495431920.1393769679
Embryo 5-20% O2217029119.99999942-Jun-98104.1876433117Embryo 5-20% O2217029119.99999942-Jun-98104.1876433117Embryo 6- 3% O201.32048708093.62985732255.84605347787.99769585249.573008183111.320037460612.839411619714.547230868217.664925910920.4212375939
Embryo 5-20% O2194602777.59999956-Jun-98144.0302869838Embryo 5-20% O2194602777.59999956-Jun-98144.0302869838Embryo 6-20% O201.32048708093.34078184314.96683153595.70379713017.0429345158.02860773359.020908208613.886827023216.9537772671
Embryo 5-20% O2423930214.399997613-Jun-98215.1535814757Embryo 5-20% O2423930214.399997613-Jun-98215.1535814757
Embryo 5-20% O22205850215.59465725-Jun-98337.5330176071Embryo 5-20% O22205850215.59465725-Jun-98337.5330176071
Embryo 5-20% O221911445474.9069811-Jul-984910.845295532Embryo 5-20% O221911445474.9069811-Jul-984910.845295532
Embryo 5-20% O269532320307.0378916-Jul-985412.5112946048Embryo 5-20% O269532320307.0378916-Jul-985412.5112946048
Embryo 5-20% O24422255571527.6108-Aug-987718.5022494652Embryo 5-20% O24422255571527.6108-Aug-987718.5022494652MEF 5
Embryo 5-20% O257430359022238.51115-Aug-988422.2012087368Embryo 5-20% O257430359022238.51115-Aug-988422.2012087368
Embryo 5-3% O211910000123-May-9800Embryo 5-3% O211910000123-May-9800
Embryo 5-3% O227720000226-May-9831.2187538443Embryo 5-3% O227720000226-May-9831.2187538443
Embryo 5-3% O2120397200329-May-9863.3375565223Embryo 5-3% O2120397200329-May-9863.3375565223
Embryo 5-3% O2551017851.99999742-Jun-98105.531853742Embryo 5-3% O2551017851.99999742-Jun-98105.531853742
Embryo 5-3% O22235295752.9466556-Jun-98147.5521485042Embryo 5-3% O22235295752.9466556-Jun-98147.5521485042
Embryo 5-3% O26981573735.036769-Jun-98179.1952350514Embryo 5-3% O26981573735.036769-Jun-98179.1952350514
Embryo 5-3% O229485513074.305713-Jun-982111.27361717Embryo 5-3% O229485513074.305713-Jun-982111.27361717
Embryo 5-3% O2102314730367.838816-Jun-982413.0685528328Embryo 5-3% O2102314730367.838816-Jun-982413.0685528328
Embryo 5-3% O2411987314281.162920-Jun-982815.0781388817Embryo 5-3% O2411987314281.162920-Jun-982815.0781388817
Embryo 5-3% O22709503236922.441026-Jun-983417.7954954319Embryo 5-3% O22709503236922.441026-Jun-983417.7954954319
Embryo 5-3% O213755244766109.61130-Jun-983820.1393769679Embryo 5-3% O213755244766109.61130-Jun-983820.1393769679
Embryo 5-3% + UV200000012-Jun-9805.531853742Embryo 6-20% O212020000123-May-9800
Embryo 5-3% + UV264000023-Jun-9815.9323916716Embryo 6-20% O230020000226-May-9831.3204870809
Embryo 5-3% + UV555000039-Jun-9877.0043415135Embryo 6-20% O2121781133.333333329-May-9863.3407818431
Embryo 5-3% + UV47767000.0000001416-Jun-981410.1097961083Embryo 6-20% O2375897764.88888842-Jun-98104.9668315359
Embryo 5-3% + UV169413626.666667520-Jun-981811.9362598533Embryo 6-20% O2626496274.81481356-Jun-98145.7037971301
Embryo 5-3% + UV1212436854.84444626-Jun-982414.7755476386Embryo 6-20% O21585035575.28148625-Jun-98337.042934515
Embryo 5-3% + UV5472131671.53123730-Jun-982816.9497409717Embryo 6-20% O23138746336.82221711-Jul-98498.0286077335
Embryo 6-20% O212020000123-May-9800Embryo 6-20% O26244079538.98765816-Jul-98549.0209082086
Embryo 6-20% O230020000226-May-9831.3204870809Embryo 6-20% O2182077359356.8898-Aug-987713.8868270232
Embryo 6-20% O2121781133.333333329-May-9863.3407818431Embryo 6-20% O21525808271410.651015-Aug-988416.9537772671
Embryo 6-20% O2375897764.88888842-Jun-98104.9668315359Embryo 6-3% O212020000123-May-9800
Embryo 6-20% O2626496274.81481356-Jun-98145.7037971301Embryo 6-3% O230020000226-May-9831.3204870809
Embryo 6-20% O21585035575.28148625-Jun-98337.042934515Embryo 6-3% O2148799133.333333329-May-9863.6298573225
Embryo 6-20% O23138746336.82221711-Jul-98498.0286077335Embryo 6-3% O2691419972.88888742-Jun-98105.8460534778
Embryo 6-20% O26244079538.98765816-Jul-98549.0209082086Embryo 6-3% O23072209412.8696256-Jun-98147.9976958524
Embryo 6-20% O2182077359356.8898-Aug-987713.8868270232Embryo 6-3% O29155184050.3514469-Jun-98179.5730081831
Embryo 6-20% O21525808271410.651015-Aug-988416.9537772671Embryo 6-3% O230730901129.013713-Jun-982111.3200374606
Embryo 6- 3% O212020000123-May-9800Embryo 6-3% O288095249903.1704816-Jun-982412.8394116197Sum of Cumm cell#Day# total
Embryo 6- 3% O230020000226-May-9831.3204870809Embryo 6-3% O2287777816350.357920-Jun-982814.5472308682Cell type03610141721242833343849547784
Embryo 6- 3% O2148799133.333333329-May-9863.6298573225Embryo 6-3% O22497911445921.091026-Jun-983417.6649259109Embryo 5-20% O2119100002772000088703999.9999996217029119.999999194602777.599999423930214.3999972205850215.5946521911445474.906969532320307.03784422255571527.657430359022238.5
Embryo 6- 3% O2691419972.88888742-Jun-98105.8460534778Embryo 6-3% O216877555002940.21130-Jun-983820.4212375939Embryo 5-3% O21191000027720000120397200551017851.9999972235295752.946656981573735.036729485513074.305102314730367.838411987314281.1622709503236922.4413755244766109.6
Embryo 6- 3% O23072209412.8696256-Jun-98147.9976958524Embryo 6-20% O21202000030020000121781133.333333375897764.888888626496274.8148131585035575.281483138746336.822216244079538.98765182077359356.881525808271410.65
Embryo 6- 3% O29155184050.3514469-Jun-98179.5730081831Embryo 6-3% O21202000030020000148799133.333333691419972.8888873072209412.869629155184050.3514430730901129.01388095249903.1704287777816350.3572497911445921.0916877555002940.2
Embryo 6- 3% O230730901129.013713-Jun-982111.3200374606
Embryo 6- 3% O288095249903.1704816-Jun-982412.8394116197
Embryo 6- 3% O2287777816350.357920-Jun-982814.5472308682
Embryo 6- 3% O22497911445921.091026-Jun-983417.6649259109
Embryo 6- 3% O216877555002940.21130-Jun-983820.4212375939
Embryo 6- 3% + UV200000012-Jun-9805.8460534778
Embryo 6- 3% + UV253200023-Jun-9816.1863308826
Embryo 6- 3% + UV558000039-Jun-9877.3263185999
Embryo 6- 3% + UV49178400416-Jun-981410.4660063709
Embryo 6- 3% + UV156387312520-Jun-981812.1350331364
Embryo 6- 3% + UV1091583437.76626-Jun-982414.9382601728
Embryo 6- 3% + UV5905466398.28157730-Jun-982817.3738887669
MEF 6
bold 3%
open 20%
Sheet2
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
Embryo 5-20% O2
Embryo 5-3% O2
Embryo 6- 3% O2
Embryo 6-20% O2
Days
Population Doublings
Sheet1
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
Embryo 5-20% O2
Embryo 5-3% O2
Embryo 6-20% O2
Embryo 6-3% O2
Days
Cummulative Cell Number
PDWorksheet
00
00
00
00
00
00
00
00
00
00
00
20%
3%
Days
Population Doublings
00
00
00
00
00
00
00
00
00
00
0
Embryo 6- 3% O2
Embryo 6-20% O2
Days
Population Doublings
9.2431113868.6599233282.29180274962.29180274961.84988161521.8498816152
9.695682944111.5450952211.52820506961.52820506964.11345175544.1134517554
14.044824660618.50027367270.90205882840.90205882844.24737156894.2473715689
17.969602915425.02284362021.66227392291.66227392295.62109280885.6210928088
10.49976072333.83028598224.34485506164.34485506165.35562804185.3556280418
18.609022556433.45863503950.27873914830.27873914831.44869034531.4486903453
14.57471264372.57798123572.5779812357
11.96507368832.87247411682.8724741168
13.11611313274.02038027144.0203802714
3%O2
20%O2
Population Doublings
Mutation Frequency (x10-5)
9.2431113868.6599233282.29180274962.29180274961.84988161521.8498816152
9.695682944111.5450952211.52820506961.52820506964.11345175544.1134517554
14.044824660618.50027367270.90205882840.90205882844.24737156894.2473715689
17.969602915425.02284362021.66227392291.66227392295.62109280885.6210928088
10.49976072333.83028598224.34485506164.34485506165.35562804185.3556280418
18.609022556433.45863503950.27873914830.27873914831.44869034531.4486903453
14.57471264372.57798123572.5779812357
11.96507368832.87247411682.8724741168
13.11611313274.02038027144.0203802714
3%O2
20%O2
Population Doublings
Mutation Frequency (x10-5)
10.56940046347.90559824652.06045891382.06045891381.03263062931.0326306293
6.37332768549.5047341791.12823378641.12823378642.29525595642.2952559564
11.58867707413.40901696895.23048614315.23048614314.56858980924.5685898092
10.637889688213.73278716223.28489319463.28489319462.74780256512.7478025651
11.09791466513.35719502010.71386190940.71386190945.89270276815.8927027681
9.31682864918.79208982010.6790505560.6790505561.90940713411.9094071341
9.942993366530.8964861741.13512629541.13512629548.84346180678.8434618067
7.927200445828.7954611741.54442810371.54442810373.83564857893.8356485789
6.3458798142.76384933352.7638493335
3%
20%
Population Doublings
Mutant Frequency (x10-5)
10.56940046347.90559824652.06045891382.06045891381.03263062931.0326306293
6.37332768549.5047341791.12823378641.12823378642.29525595642.2952559564
11.58867707413.40901696895.23048614315.23048614314.56858980924.5685898092
10.637889688213.73278716223.28489319463.28489319462.74780256512.7478025651
11.09791466513.35719502010.71386190940.71386190945.89270276815.8927027681
9.31682864918.79208982010.6790505560.6790505561.90940713411.9094071341
9.942993366530.8964861741.13512629541.13512629548.84346180678.8434618067
7.927200445828.7954611741.54442810371.54442810373.83564857893.8356485789
6.3458798142.76384933352.7638493335
3%
20%
Population Doublings
Mutant Frequency (x10-5)
00
00
00
00
00
00
00
00
00
00
0
Embryo 6- 3% O2
Embryo 6-20% O2
Days
Population Doublings
Embryo 5-20% O2DATA
Cumm cell#PassageDateDay# totalPD#total
11910000123-May-9800
27720000226-May-9831.2187538443
88703999.9999996329-May-9862.8968257494
217029119.99999942-Jun-98104.1876433117
194602777.59999956-Jun-98144.0302869838
423930214.399997613-Jun-98215.1535814757
2205850215.59465725-Jun-98337.5330176071
21911445474.9069811-Jul-984910.845295532
69532320307.0378916-Jul-985412.5112946048
4422255571527.6108-Aug-987718.5022494652
57430359022238.51115-Aug-988422.2012087368
Embryo 5-3% O2DATA
Cumm cell#PassageDateDay# totalPD#total
11910000123-May-9800
27720000226-May-9831.2187538443
120397200329-May-9863.3375565223
551017851.99999742-Jun-98105.531853742
2235295752.9466556-Jun-98147.5521485042
6981573735.036769-Jun-98179.1952350514
29485513074.305713-Jun-982111.27361717
102314730367.838816-Jun-982413.0685528328
411987314281.162920-Jun-982815.0781388817
2709503236922.441026-Jun-983417.7954954319
13755244766109.61130-Jun-983820.1393769679
Embryo 5-3% + UVDATA
Cumm cell#PassageDateDay# totalPD#total
200000012-Jun-9805.531853742
264000023-Jun-9815.9323916716
555000039-Jun-9877.0043415135
47767000.0000001416-Jun-981410.1097961083
169413626.666667520-Jun-981811.9362598533
1212436854.84444626-Jun-982414.7755476386
5472131671.53123730-Jun-982816.9497409717
Embryo 6-20% O2DATA
Cumm cell#PassageDateDay# totalPD#total
12020000123-May-9800
30020000226-May-9831.3204870809
121781133.333333329-May-9863.3407818431
375897764.88888842-Jun-98104.9668315359
626496274.81481356-Jun-98145.7037971301
1585035575.28148625-Jun-98337.042934515
3138746336.82221711-Jul-98498.0286077335
6244079538.98765816-Jul-98549.0209082086
182077359356.8898-Aug-987713.8868270232
1525808271410.651015-Aug-988416.9537772671
Embryo 6- 3% O2DATA
Cumm cell#PassageDateDay# totalPD#total
12020000123-May-9800
30020000226-May-9831.3204870809
148799133.333333329-May-9863.6298573225
691419972.88888742-Jun-98105.8460534778
3072209412.8696256-Jun-98147.9976958524
9155184050.3514469-Jun-98179.5730081831
30730901129.013713-Jun-982111.3200374606
88095249903.1704816-Jun-982412.8394116197
287777816350.357920-Jun-982814.5472308682
2497911445921.091026-Jun-983417.6649259109
16877555002940.21130-Jun-983820.4212375939
Embryo 6- 3% + UVDATA
Cumm cell#PassageDateDay# totalPD#total
200000012-Jun-9805.8460534778
253200023-Jun-9816.1863308826
558000039-Jun-9877.3263185999
49178400416-Jun-981410.4660063709
156387312520-Jun-981812.1350331364
1091583437.76626-Jun-982414.9382601728
5905466398.28157730-Jun-982817.3738887669
Embryo 5-20% O2DATADATADATA
Cumm cell#PassageDateDay# totalDay#increm>Days/>PDCell# (out)PD#totalPD# incremCell# (in)# of T150 flasks# of pelletscell# per pelletLabel in the tubes
119100001 (thawed)23-May-9800000119100002 in 3% O2
27720000226-May-98332.4615306972277200001.21875384431.2187538443300000030Rest of cells frozen to repeat
88703999.9999998329-May-98631.787766061196000002.89682574941.678071905130000003166000005A-20%
217029119.99999942-Jun-981043.098811262573400004.18764331171.290817562330000003143400005B-20%
194602777.59999956-Jun-98144-25.420013623826900004.0302869838-0.1573563279269000030
423930214.399998613-Jun-982176.231669478258600005.15358147571.12329449199000003149600005C-20%(senesc)
2205850215.59466725-Jun-9833125.043211642146830007.53301760712.37943613156000003140830005D-20%
21911445474.9069811-Jul-9849164.8305125243596000010.8452955323.312277924915000003144600005E - 20%
69532320307.0379916-Jul-985453.0012021505476000012.51129460481.66599907281500003146100005F-20%
4422255571527.61108-Aug-9877233.8391208974954000018.50224946525.990954860415000003180400005G-20%
57430359022238.61115-Aug-988471.89242418911948000022.20120873683.69895927164000000Frozen1154800005H-20%
Embryo 5-3% O2DATADATADATA
Cumm cell#PassageDateDay# totalDay#increm>Days/>PDCell# (out)PD#totalPD# incremCell# (in)# of T150 flasks# of pelletscell# per pelletLabel in the tubes
119100001 (thawed)23-May-9800000119100002 in 3% O2
27720000226-May-98332.4615306972277200001.21875384431.2187538443300000030Rest of cells frozen to repeat
120397200329-May-98631.4158940004130300003.33755652232.1188026781300000031100300005A-3%
55101785242-Jun-981041.8229071086137300005.5318537422.194297219730000003 and UV (1)177300005B-3%
2235295752.9466756-Jun-981441.9799091077121700007.55214850422.020294762230000003191700005C-3%
6981573735.0367569-Jun-981731.825832002193700009.19523505141.643086547230000003163700005D-3%
29485513074.3052713-Jun-982141.92457390971267000011.273617172.078382118630000003196700005E - 3%
102314730367.839816-Jun-982431.67136909821041000013.06855283281.794935662830000003174100005F-3%
411987314281.164920-Jun-982841.99045967821208000015.07813888172.009586048830000003190800005G-3%
2709503236922.451026-Jun-983462.20802823961973000017.79549543192.7173565502300000031167300005H-3%
13755244766109.61130-Jun-983841.70657089051523000020.13937696792.3438815364000000Frozen1112300005I-3%
Embryo 5-3% + UVDATADATADATA
Cumm cell#PassageDateDay# totalDay#increm>Days/>PDCell# (out)PD#totalPD# incremCell# (in)# of T150 flasks# of pelletscell# per pelletLabel in the tubes
20000001 (see 3% O2)2-Jun-980005.531853742020000001
2640000UV before passage 23-Jun-98112.496642455426400005.93239167160.4005379296264000030
555000039-Jun-98765.59727681855500007.00434151351.071949841915000003140500005A-UV
47767000416-Jun-981472.25409832481291000010.10979610833.105454594830000003199100005B-UV
169413626.666667520-Jun-981842.19002430841064000011.93625985331.82646374530000003176400005C-UV
1212436854.84444626-Jun-982462.11320600572147000014.77554763862.8392877854300000031184700005D-UV
5472131671.53125730-Jun-982841.83976279351354000016.94974097172.17419333314000000Frozen195400005E - UV
Embryo 6-20% O2DATADATADATA
Cumm cell#PassageDateDay# totalDay#increm>Days/>PDCell# (out)PD#totalPD# incremCell# (in)# of T150 flasks# of pelletscell# per pelletLabel in the tubes
120200001 (thawed)23-May-9800000120200002 in 3% O2
30020000226-May-98332.2718889441300200001.32048708091.3204870809300000030Rest of cells frozen to repeat
121781133.333334329-May-98631.4849318308121700003.34078184312.020294762230000003191700006A-20%
375897764.88888942-Jun-981042.459949420992600004.96683153591.626049692830000003162600006B-20%
626496274.81481656-Jun-981445.427661795450000005.70379713010.73696559423000003147000006C-20%(senesc)
1585035575.28148625-Jun-98331914.18823805087590007.0429345151.339137384975900030
3138746336.82223711-Jul-98491616.232560345215030008.02860773350.9856732185150300030
6244079538.98766816-Jul-985455.038796337529900009.02090820860.99230047521500003128400006D-20%
182077359356.8898-Aug-9877234.7267537492437400013.88682702324.865918814615000003128740006E - 20%
1525808271410.661015-Aug-988472.28239764041257000016.95377726713.06695024394000000Frozen185700006F-20%
Embryo 6- 3% O2DATADATADATA
Cumm cell#PassageDateDay# totalDay#increm>Days/>PDCell# (out)PD#totalPD# incremCell# (in)# of T150 flasks# of pelletscell# per pelletLabel in the tubes
120200001 (thawed)23-May-9800000120200002 in 3% O2
30020000226-May-98332.2718889441300200001.32048708091.3204870809300000030Rest of cells frozen to repeat
148799133.333334329-May-98631.2990554507148700003.62985732252.3093702416300000031118700006A-3%
691419972.8888942-Jun-981041.8048943864139400005.84605347782.216196155430000003 and UV (1)179400006B-3%
3072209412.8696356-Jun-981441.8590450008133300007.99769585242.1516423746300000031103300006C-3%
9155184050.3515169-Jun-981731.904384255589400009.57300818311.575312330730000003159400006D-3%
30730901129.0132713-Jun-982142.2896010111007000011.32003746061.747029277530000003170700006E - 3%
88095249903.1712816-Jun-982431.9744971849860000012.83941161971.519374159130000003156000006F-3%
287777816350.359920-Jun-982842.3421682379980000014.54723086821.707819248530000003168000006G-3%
2497911445921.121026-Jun-983461.92449868182604000017.66492591093.1176950427300000031230400006H-3%
16877555002940.31130-Jun-983841.45121468842027000020.42123759392.7563116834000000Frozen1162700006I-3%
Embryo 6- 3% + UVDATADATADATA
Cumm cell#PassageDateDay# totalDay#increm>Days/>PDCell# (out)PD#totalPD# incremCell# (in)# of T150 flasks# of pelletscell# per pelletLabel in the tubes
20000001 (see 3% O2)2-Jun-980005.8460534778020000001
2532000UV before passage 23-Jun-98112.938778731725320006.18633088260.3402774048253200030
558000039-Jun-98765.263214602255800007.32631859991.139987717315000003140800006A-UV
49178400416-Jun-981472.22952105771322000010.46600637093.139687771300000031102200006B-UV
156387312520-Jun-981842.3966062634954000012.13503313641.669026765530000003165400006C-UV
1091583437.76626-Jun-982462.14039031522094000014.93826017282.8032270364300000031179400006D-UV
5905466398.28159730-Jun-982841.64228651681623000017.37388876692.43562859414000000Frozen1122300006E - UV
&F
Page &P
Chart1
8.85798174493.74003673675.1179450082
11.4468976485.86304513685.5838525112
8.47950825873.01493626985.4645719889
23.458915893910.612366713912.84654918
32.064525246223.86197227638.20255297
3%O2
20%O2
total
no-change
size-change
Population Doublings
Mutant Frequency (x10 -5)
Sheet1
Sheet1
8.85798174493.74003673675.1179450082
11.4468976485.86304513685.5838525112
8.47950825873.01493626985.4645719889
23.458915893910.612366713912.84654918
32.064525246223.86197227638.20255297
3%O2
20%O2
total
no-change
size-change
Population Doublings
Mutant Frequency (x10 -5)
Sheet2
Sheet3
MBD04DB7550.xls
Chart8
9.2431113868.6599233282.29180274962.29180274961.84988161521.8498816152
9.695682944111.5450952211.52820506961.52820506964.11345175544.1134517554
14.044824660618.50027367270.90205882840.90205882844.24737156894.2473715689
17.969602915425.02284362021.66227392291.66227392295.62109280885.6210928088
10.49976072333.83028598224.34485506164.34485506165.35562804185.3556280418
18.609022556433.45863503950.27873914830.27873914831.44869034531.4486903453
14.57471264372.57798123572.5779812357
11.96507368832.87247411682.8724741168
13.11611313274.02038027144.0203802714
3%O2
20%O2
Population Doublings
Mutant Frequency (x10-5)
Sheet3
Cell typeDateDay# totalPD#total
Embryo 5-3% O223-May-9800
Embryo 5-3% O226-May-9831.2187538443
Embryo 5-3% O229-May-9863.3375565223
Embryo 5-3% O22-Jun-98105.531853742
Embryo 5-3% O26-Jun-98147.5521485042Sum of PD#totalDay# total
Embryo 5-3% O29-Jun-98179.1952350514Cell type036101114172124283438
Embryo 5-3% O213-Jun-982111.27361717Embryo 5-3% + UV01.21875384433.33755652235.5318537425.93239167167.004341513510.109796108311.936259853314.775547638616.9497409717
Embryo 5-3% O216-Jun-982413.0685528328Embryo 5-3% O201.21875384433.33755652235.5318537427.55214850429.195235051411.2736171713.068552832815.078138881717.795495431920.1393769679
Embryo 5-3% O220-Jun-982815.0781388817Embryo 6- 3% + UV01.32048708093.62985732255.84605347786.18633088267.326318599910.466006370912.135033136414.938260172817.3738887669
Embryo 5-3% O226-Jun-983417.7954954319Embryo 6- 3% O201.32048708093.62985732255.84605347787.99769585249.573008183111.320037460612.839411619714.547230868217.664925910920.4212375939
Embryo 5-3% O230-Jun-983820.1393769679
Embryo 5-3% + UV23-May-9800
Embryo 5-3% + UV26-May-9831.2187538443
Embryo 5-3% + UV29-May-9863.3375565223
Embryo 5-3% + UV2-Jun-98105.531853742
Embryo 5-3% + UV3-Jun-98115.9323916716
Embryo 5-3% + UV9-Jun-98177.0043415135
Embryo 5-3% + UV16-Jun-982410.1097961083
Embryo 5-3% + UV20-Jun-982811.9362598533
Embryo 5-3% + UV26-Jun-983414.7755476386
Embryo 5-3% + UV30-Jun-983816.9497409717
Embryo 6- 3% O223-May-9800
Embryo 6- 3% O226-May-9831.3204870809
Embryo 6- 3% O229-May-9863.6298573225
Embryo 6- 3% O22-Jun-98105.8460534778
Embryo 6- 3% O26-Jun-98147.9976958524
Embryo 6- 3% O29-Jun-98179.5730081831
Embryo 6- 3% O213-Jun-982111.3200374606
Embryo 6- 3% O216-Jun-982412.8394116197
Embryo 6- 3% O220-Jun-982814.5472308682
Embryo 6- 3% O226-Jun-983417.6649259109
Embryo 6- 3% O230-Jun-983820.4212375939
Embryo 6- 3% + UV23-May-9800
Embryo 6- 3% + UV26-May-9831.3204870809
Embryo 6- 3% + UV29-May-9863.6298573225
Embryo 6- 3% + UV2-Jun-98105.8460534778
Embryo 6- 3% + UV3-Jun-98116.1863308826
Embryo 6- 3% + UV9-Jun-98177.3263185999
Embryo 6- 3% + UV16-Jun-982410.4660063709
Embryo 6- 3% + UV20-Jun-982812.1350331364
Embryo 6- 3% + UV26-Jun-983414.9382601728
Embryo 6- 3% + UV30-Jun-983817.3738887669
Sheet3
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
Embryo 5-3% + UV
Embryo 5-3% O2
Embryo 6- 3% + UV
Embryo 6- 3% O2
days
Population doublings
Sheet2
Cell typeCumm cell#PassageDateDay# totalPD#totalCell typeCumm cell#PassageDateDay# totalPD#totalSum of PD#totalDay# total
Embryo 5-20% O211910000123-May-9800Embryo 5-20% O211910000123-May-9800Cell type03610141721242833343849547784
Embryo 5-20% O227720000226-May-9831.2187538443Embryo 5-20% O227720000226-May-9831.2187538443Embryo 5-20% O201.21875384432.89682574944.18764331174.03028698385.15358147577.533017607110.84529553212.511294604818.502249465222.2012087368
Embryo 5-20% O288703999.9999996329-May-9862.8968257494Embryo 5-20% O288703999.9999996329-May-9862.8968257494Embryo 5-3% O201.21875384433.33755652235.5318537427.55214850429.195235051411.2736171713.068552832815.078138881717.795495431920.1393769679
Embryo 5-20% O2217029119.99999942-Jun-98104.1876433117Embryo 5-20% O2217029119.99999942-Jun-98104.1876433117Embryo 6- 3% O201.32048708093.62985732255.84605347787.99769585249.573008183111.320037460612.839411619714.547230868217.664925910920.4212375939
Embryo 5-20% O2194602777.59999956-Jun-98144.0302869838Embryo 5-20% O2194602777.59999956-Jun-98144.0302869838Embryo 6-20% O201.32048708093.34078184314.96683153595.70379713017.0429345158.02860773359.020908208613.886827023216.9537772671
Embryo 5-20% O2423930214.399997613-Jun-98215.1535814757Embryo 5-20% O2423930214.399997613-Jun-98215.1535814757
Embryo 5-20% O22205850215.59465725-Jun-98337.5330176071Embryo 5-20% O22205850215.59465725-Jun-98337.5330176071
Embryo 5-20% O221911445474.9069811-Jul-984910.845295532Embryo 5-20% O221911445474.9069811-Jul-984910.845295532
Embryo 5-20% O269532320307.0378916-Jul-985412.5112946048Embryo 5-20% O269532320307.0378916-Jul-985412.5112946048
Embryo 5-20% O24422255571527.6108-Aug-987718.5022494652Embryo 5-20% O24422255571527.6108-Aug-987718.5022494652MEF 5
Embryo 5-20% O257430359022238.51115-Aug-988422.2012087368Embryo 5-20% O257430359022238.51115-Aug-988422.2012087368
Embryo 5-3% O211910000123-May-9800Embryo 5-3% O211910000123-May-9800
Embryo 5-3% O227720000226-May-9831.2187538443Embryo 5-3% O227720000226-May-9831.2187538443
Embryo 5-3% O2120397200329-May-9863.3375565223Embryo 5-3% O2120397200329-May-9863.3375565223
Embryo 5-3% O2551017851.99999742-Jun-98105.531853742Embryo 5-3% O2551017851.99999742-Jun-98105.531853742
Embryo 5-3% O22235295752.9466556-Jun-98147.5521485042Embryo 5-3% O22235295752.9466556-Jun-98147.5521485042
Embryo 5-3% O26981573735.036769-Jun-98179.1952350514Embryo 5-3% O26981573735.036769-Jun-98179.1952350514
Embryo 5-3% O229485513074.305713-Jun-982111.27361717Embryo 5-3% O229485513074.305713-Jun-982111.27361717
Embryo 5-3% O2102314730367.838816-Jun-982413.0685528328Embryo 5-3% O2102314730367.838816-Jun-982413.0685528328
Embryo 5-3% O2411987314281.162920-Jun-982815.0781388817Embryo 5-3% O2411987314281.162920-Jun-982815.0781388817
Embryo 5-3% O22709503236922.441026-Jun-983417.7954954319Embryo 5-3% O22709503236922.441026-Jun-983417.7954954319
Embryo 5-3% O213755244766109.61130-Jun-983820.1393769679Embryo 5-3% O213755244766109.61130-Jun-983820.1393769679
Embryo 5-3% + UV200000012-Jun-9805.531853742Embryo 6-20% O212020000123-May-9800
Embryo 5-3% + UV264000023-Jun-9815.9323916716Embryo 6-20% O230020000226-May-9831.3204870809
Embryo 5-3% + UV555000039-Jun-9877.0043415135Embryo 6-20% O2121781133.333333329-May-9863.3407818431
Embryo 5-3% + UV47767000.0000001416-Jun-981410.1097961083Embryo 6-20% O2375897764.88888842-Jun-98104.9668315359
Embryo 5-3% + UV169413626.666667520-Jun-981811.9362598533Embryo 6-20% O2626496274.81481356-Jun-98145.7037971301
Embryo 5-3% + UV1212436854.84444626-Jun-982414.7755476386Embryo 6-20% O21585035575.28148625-Jun-98337.042934515
Embryo 5-3% + UV5472131671.53123730-Jun-982816.9497409717Embryo 6-20% O23138746336.82221711-Jul-98498.0286077335
Embryo 6-20% O212020000123-May-9800Embryo 6-20% O26244079538.98765816-Jul-98549.0209082086
Embryo 6-20% O230020000226-May-9831.3204870809Embryo 6-20% O2182077359356.8898-Aug-987713.8868270232
Embryo 6-20% O2121781133.333333329-May-9863.3407818431Embryo 6-20% O21525808271410.651015-Aug-988416.9537772671
Embryo 6-20% O2375897764.88888842-Jun-98104.9668315359Embryo 6-3% O212020000123-May-9800
Embryo 6-20% O2626496274.81481356-Jun-98145.7037971301Embryo 6-3% O230020000226-May-9831.3204870809
Embryo 6-20% O21585035575.28148625-Jun-98337.042934515Embryo 6-3% O2148799133.333333329-May-9863.6298573225
Embryo 6-20% O23138746336.82221711-Jul-98498.0286077335Embryo 6-3% O2691419972.88888742-Jun-98105.8460534778
Embryo 6-20% O26244079538.98765816-Jul-98549.0209082086Embryo 6-3% O23072209412.8696256-Jun-98147.9976958524
Embryo 6-20% O2182077359356.8898-Aug-987713.8868270232Embryo 6-3% O29155184050.3514469-Jun-98179.5730081831
Embryo 6-20% O21525808271410.651015-Aug-988416.9537772671Embryo 6-3% O230730901129.013713-Jun-982111.3200374606
Embryo 6- 3% O212020000123-May-9800Embryo 6-3% O288095249903.1704816-Jun-982412.8394116197Sum of Cumm cell#Day# total
Embryo 6- 3% O230020000226-May-9831.3204870809Embryo 6-3% O2287777816350.357920-Jun-982814.5472308682Cell type03610141721242833343849547784
Embryo 6- 3% O2148799133.333333329-May-9863.6298573225Embryo 6-3% O22497911445921.091026-Jun-983417.6649259109Embryo 5-20% O2119100002772000088703999.9999996217029119.999999194602777.599999423930214.3999972205850215.5946521911445474.906969532320307.03784422255571527.657430359022238.5
Embryo 6- 3% O2691419972.88888742-Jun-98105.8460534778Embryo 6-3% O216877555002940.21130-Jun-983820.4212375939Embryo 5-3% O21191000027720000120397200551017851.9999972235295752.946656981573735.036729485513074.305102314730367.838411987314281.1622709503236922.4413755244766109.6
Embryo 6- 3% O23072209412.8696256-Jun-98147.9976958524Embryo 6-20% O21202000030020000121781133.333333375897764.888888626496274.8148131585035575.281483138746336.822216244079538.98765182077359356.881525808271410.65
Embryo 6- 3% O29155184050.3514469-Jun-98179.5730081831Embryo 6-3% O21202000030020000148799133.333333691419972.8888873072209412.869629155184050.3514430730901129.01388095249903.1704287777816350.3572497911445921.0916877555002940.2
Embryo 6- 3% O230730901129.013713-Jun-982111.3200374606
Embryo 6- 3% O288095249903.1704816-Jun-982412.8394116197
Embryo 6- 3% O2287777816350.357920-Jun-982814.5472308682
Embryo 6- 3% O22497911445921.091026-Jun-983417.6649259109
Embryo 6- 3% O216877555002940.21130-Jun-983820.4212375939
Embryo 6- 3% + UV200000012-Jun-9805.8460534778
Embryo 6- 3% + UV253200023-Jun-9816.1863308826
Embryo 6- 3% + UV558000039-Jun-9877.3263185999
Embryo 6- 3% + UV49178400416-Jun-981410.4660063709
Embryo 6- 3% + UV156387312520-Jun-981812.1350331364
Embryo 6- 3% + UV1091583437.76626-Jun-982414.9382601728
Embryo 6- 3% + UV5905466398.28157730-Jun-982817.3738887669
MEF 6
bold 3%
open 20%
Sheet2
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
Embryo 5-20% O2
Embryo 5-3% O2
Embryo 6- 3% O2
Embryo 6-20% O2
Days
Population Doublings
Sheet1
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
0000
Embryo 5-20% O2
Embryo 5-3% O2
Embryo 6-20% O2
Embryo 6-3% O2
Days
Cummulative Cell Number
PDWorksheet
00
00
00
00
00
00
00
00
00
00
00
20%
3%
Days
Population Doublings
00
00
00
00
00
00
00
00
00
00
0
Embryo 6- 3% O2
Embryo 6-20% O2
Days
Population Doublings
9.2431113868.6599233282.29180274962.29180274961.84988161521.8498816152
9.695682944111.5450952211.52820506961.52820506964.11345175544.1134517554
14.044824660618.50027367270.90205882840.90205882844.24737156894.2473715689
17.969602915425.02284362021.66227392291.66227392295.62109280885.6210928088
10.49976072333.83028598224.34485506164.34485506165.35562804185.3556280418
18.609022556433.45863503950.27873914830.27873914831.44869034531.4486903453
14.57471264372.57798123572.5779812357
11.96507368832.87247411682.8724741168
13.11611313274.02038027144.0203802714
3%O2
20%O2
Population Doublings
Mutation Frequency (x10-5)
9.2431113868.6599233282.29180274962.29180274961.84988161521.8498816152
9.695682944111.5450952211.52820506961.52820506964.11345175544.1134517554
14.044824660618.50027367270.90205882840.90205882844.24737156894.2473715689
17.969602915425.02284362021.66227392291.66227392295.62109280885.6210928088
10.49976072333.83028598224.34485506164.34485506165.35562804185.3556280418
18.609022556433.45863503950.27873914830.27873914831.44869034531.4486903453
14.57471264372.57798123572.5779812357
11.96507368832.87247411682.8724741168
13.11611313274.02038027144.0203802714
3%O2
20%O2
Population Doublings
Mutation Frequency (x10-5)
10.56940046347.90559824652.06045891382.06045891381.03263062931.0326306293
6.37332768549.5047341791.12823378641.12823378642.29525595642.2952559564
11.58867707413.40901696895.23048614315.23048614314.56858980924.5685898092
10.637889688213.73278716223.28489319463.28489319462.74780256512.7478025651
11.09791466513.35719502010.71386190940.71386190945.89270276815.8927027681
9.31682864918.79208982010.6790505560.6790505561.90940713411.9094071341
9.942993366530.8964861741.13512629541.13512629548.84346180678.8434618067
7.927200445828.7954611741.54442810371.54442810373.83564857893.8356485789
6.3458798142.76384933352.7638493335
3%
20%
Population Doublings
Mutant Frequency (x10-5)
10.56940046347.90559824652.06045891382.06045891381.03263062931.0326306293
6.37332768549.5047341791.12823378641.12823378642.29525595642.2952559564
11.58867707413.40901696895.23048614315.23048614314.56858980924.5685898092
10.637889688213.73278716223.28489319463.28489319462.74780256512.7478025651
11.09791466513.35719502010.71386190940.71386190945.89270276815.8927027681
9.31682864918.79208982010.6790505560.6790505561.90940713411.9094071341
9.942993366530.8964861741.13512629541.13512629548.84346180678.8434618067
7.927200445828.7954611741.54442810371.54442810373.83564857893.8356485789
6.3458798142.76384933352.7638493335
3%
20%
Population Doublings
Mutant Frequency (x10-5)
00
00
00
00
00
00
00
00
00
00
0
Embryo 6- 3% O2
Embryo 6-20% O2
Days
Population Doublings
Embryo 5-20% O2DATA
Cumm cell#PassageDateDay# totalPD#total
11910000123-May-9800
27720000226-May-9831.2187538443
88703999.9999996329-May-9862.8968257494
217029119.99999942-Jun-98104.1876433117
194602777.59999956-Jun-98144.0302869838
423930214.399997613-Jun-98215.1535814757
2205850215.59465725-Jun-98337.5330176071
21911445474.9069811-Jul-984910.845295532
69532320307.0378916-Jul-985412.5112946048
4422255571527.6108-Aug-987718.5022494652
57430359022238.51115-Aug-988422.2012087368
Embryo 5-3% O2DATA
Cumm cell#PassageDateDay# totalPD#total
11910000123-May-9800
27720000226-May-9831.2187538443
120397200329-May-9863.3375565223
551017851.99999742-Jun-98105.531853742
2235295752.9466556-Jun-98147.5521485042
6981573735.036769-Jun-98179.1952350514
29485513074.305713-Jun-982111.27361717
102314730367.838816-Jun-982413.0685528328
411987314281.162920-Jun-982815.0781388817
2709503236922.441026-Jun-983417.7954954319
13755244766109.61130-Jun-983820.1393769679
Embryo 5-3% + UVDATA
Cumm cell#PassageDateDay# totalPD#total
200000012-Jun-9805.531853742
264000023-Jun-9815.9323916716
555000039-Jun-9877.0043415135
47767000.0000001416-Jun-981410.1097961083
169413626.666667520-Jun-981811.9362598533
1212436854.84444626-Jun-982414.7755476386
5472131671.53123730-Jun-982816.9497409717
Embryo 6-20% O2DATA
Cumm cell#PassageDateDay# totalPD#total
12020000123-May-9800
30020000226-May-9831.3204870809
121781133.333333329-May-9863.3407818431
375897764.88888842-Jun-98104.9668315359
626496274.81481356-Jun-98145.7037971301
1585035575.28148625-Jun-98337.042934515
3138746336.82221711-Jul-98498.0286077335
6244079538.98765816-Jul-98549.0209082086
182077359356.8898-Aug-987713.8868270232
1525808271410.651015-Aug-988416.9537772671
Embryo 6- 3% O2DATA
Cumm cell#PassageDateDay# totalPD#total
12020000123-May-9800
30020000226-May-9831.3204870809
148799133.333333329-May-9863.6298573225
691419972.88888742-Jun-98105.8460534778
3072209412.8696256-Jun-98147.9976958524
9155184050.3514469-Jun-98179.5730081831
30730901129.013713-Jun-982111.3200374606
88095249903.1704816-Jun-982412.8394116197
287777816350.357920-Jun-982814.5472308682
2497911445921.091026-Jun-983417.6649259109
16877555002940.21130-Jun-983820.4212375939
Embryo 6- 3% + UVDATA
Cumm cell#PassageDateDay# totalPD#total
200000012-Jun-9805.8460534778
253200023-Jun-9816.1863308826
558000039-Jun-9877.3263185999
49178400416-Jun-981410.4660063709
156387312520-Jun-981812.1350331364
1091583437.76626-Jun-982414.9382601728
5905466398.28157730-Jun-982817.3738887669
Embryo 5-20% O2DATADATADATA
Cumm cell#PassageDateDay# totalDay#increm>Days/>PDCell# (out)PD#totalPD# incremCell# (in)# of T150 flasks# of pelletscell# per pelletLabel in the tubes
119100001 (thawed)23-May-9800000119100002 in 3% O2
27720000226-May-98332.4615306972277200001.21875384431.2187538443300000030Rest of cells frozen to repeat
88703999.9999998329-May-98631.787766061196000002.89682574941.678071905130000003166000005A-20%
217029119.99999942-Jun-981043.098811262573400004.18764331171.290817562330000003143400005B-20%
194602777.59999956-Jun-98144-25.420013623826900004.0302869838-0.1573563279269000030
423930214.399998613-Jun-982176.231669478258600005.15358147571.12329449199000003149600005C-20%(senesc)
2205850215.59466725-Jun-9833125.043211642146830007.53301760712.37943613156000003140830005D-20%
21911445474.9069811-Jul-9849164.8305125243596000010.8452955323.312277924915000003144600005E - 20%
69532320307.0379916-Jul-985453.0012021505476000012.51129460481.66599907281500003146100005F-20%
4422255571527.61108-Aug-9877233.8391208974954000018.50224946525.990954860415000003180400005G-20%
57430359022238.61115-Aug-988471.89242418911948000022.20120873683.69895927164000000Frozen1154800005H-20%
Embryo 5-3% O2DATADATADATA
Cumm cell#PassageDateDay# totalDay#increm>Days/>PDCell# (out)PD#totalPD# incremCell# (in)# of T150 flasks# of pelletscell# per pelletLabel in the tubes
119100001 (thawed)23-May-9800000119100002 in 3% O2
27720000226-May-98332.4615306972277200001.21875384431.2187538443300000030Rest of cells frozen to repeat
120397200329-May-98631.4158940004130300003.33755652232.1188026781300000031100300005A-3%
55101785242-Jun-981041.8229071086137300005.5318537422.194297219730000003 and UV (1)177300005B-3%
2235295752.9466756-Jun-981441.9799091077121700007.55214850422.020294762230000003191700005C-3%
6981573735.0367569-Jun-981731.825832002193700009.19523505141.643086547230000003163700005D-3%
29485513074.3052713-Jun-982141.92457390971267000011.273617172.078382118630000003196700005E - 3%
102314730367.839816-Jun-982431.67136909821041000013.06855283281.794935662830000003174100005F-3%
411987314281.164920-Jun-982841.99045967821208000015.07813888172.009586048830000003190800005G-3%
2709503236922.451026-Jun-983462.20802823961973000017.79549543192.7173565502300000031167300005H-3%
13755244766109.61130-Jun-983841.70657089051523000020.13937696792.3438815364000000Frozen1112300005I-3%
Embryo 5-3% + UVDATADATADATA
Cumm cell#PassageDateDay# totalDay#increm>Days/>PDCell# (out)PD#totalPD# incremCell# (in)# of T150 flasks# of pelletscell# per pelletLabel in the tubes
20000001 (see 3% O2)2-Jun-980005.531853742020000001
2640000UV before passage 23-Jun-98112.496642455426400005.93239167160.4005379296264000030
555000039-Jun-98765.59727681855500007.00434151351.071949841915000003140500005A-UV
47767000416-Jun-981472.25409832481291000010.10979610833.105454594830000003199100005B-UV
169413626.666667520-Jun-981842.19002430841064000011.93625985331.82646374530000003176400005C-UV
1212436854.84444626-Jun-982462.11320600572147000014.77554763862.8392877854300000031184700005D-UV
5472131671.53125730-Jun-982841.83976279351354000016.94974097172.17419333314000000Frozen195400005E - UV
Embryo 6-20% O2DATADATADATA
Cumm cell#PassageDateDay# totalDay#increm>Days/>PDCell# (out)PD#totalPD# incremCell# (in)# of T150 flasks# of pelletscell# per pelletLabel in the tubes
120200001 (thawed)23-May-9800000120200002 in 3% O2
30020000226-May-98332.2718889441300200001.32048708091.
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