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GENETIC DIVERSITY OF THE NORTH MEDITERRANEAN
POPULATIONS OF CHONDROSTOMA (CYPRINIDAE)
2Jasna Vukić, 1Radek Šanda
PHYLOGEOGRAPHY OF THE NORTH MEDITERRANEAN
POPULATIONS OF ALBURNUS ALBURNUS COMPLEX
(CYPRINIDAE)
1Radek Šanda, 2Jasna Vukić, 3Ivana Buj, 4Ivan Bogut
1National Museum in Prague, Department of Zoology2Department of Ecology, Faculty of Science, Charles University, Prague
3Department of Zoology, Faculty of Science, University of Zagreb4Institute of Special Zootechniques, Josi Juraj Strossmayer University, Osijek, Croatia
Chondrostoma nasus
genus Chondrostoma: bentic fishes, lower mouth with
horny layer on lower lip, important in recreational fisheries
- recently considerable taxonomic revision
Alburnus scoranza
genus Alburnus: pelagic fishes, upper mouth, important locally
in commercial fisheries (Prespa, Skadar, Ohrid lakes)
- recently considerable taxonomic revision
Alburnus neretvae Buj, Šanda & Perea, 2010
Buj, I., Vukić, J., Šanda, R., Perea, S., Ćaleta,
M., Marčić, Z., Bogut, I., Povž, M. &
Mrakovčić, M. Morphological comparison of
bleaks (Alburnus, Cyprinidae) from the
Adriatic Basin with the description of a new
species. Folia Zoologica, 2010, 59 (2): 129-
141.
Aims
to study the genetic diversity of Alburnus and
Chondrostoma populations from the area of the
northern Mediterranean, and to resolve their
phylogenetic relationships and taxonomy
Methods
• extraction of DNA
• amplification of mitochondrial cytochrome b gene
• sequencing
• model of evolution (Modeltest)
• Bayesian methods of phylogenetic inference (Mr. Bayes)
• haplotype network (Network)
Distribution of European species of genus Chondrostoma according to Elvira
(1987) and Kottelat and Freyhof (2007)
further several species in Near and Middle East nad Caspian basin
Chondrostoma
C. nasus
C. phoxinusC. knerii
C. prespense
C. vardarense
C. soetta
C. kubanicum
C. scodrense
Additional sequences available from GenBank: C. angorense, C. soetta,
C. regium, C. oxyrhynchum, C. cyri, C. holmwoodii, C. meandrense
Sampling localities of
new material analysed
in this study:
C. nasus
C. ohridana
C. knerii
C. phoxinus
C. prespense
C. vardarense
TA2 93Tsouf iaB2 9Ppseu da
B7 1Palep
DQ44 7739 Chkn er iiA19 18
A19 19AY56 8623 Chsoetta
AY56 8624 ChsoettaAF5 3376 7Chsoetta
AY49 4741 Chph oxinus
B8 4Chp hoxCP 1Chp hox
AY56 8622 ChgeneiAF5 3376 6Chg enei
AF5 3376 2Chh olmwoodiiAF5 3376 5Chh olmwoodii
AF5 3376 4Chmean drenseAF5 3376 3Chmean drense
AF5 3376 0CHangor en se
Z7 5109 Chnasu sA12 74
A12 75A12 76
A12 77A12 79Sik1 A
UBL5CUBL5D
A12 78OLS 16
A23 06A23 07
A23 08A23 10A23 11
A23 12A23 13
Hor 11A23 04
A23 05A23 09B3 3A
ULI 8AY02 6402 Chnasu s
AF0 9074 9ChAo osDr im3
Dr im7Dr im8
Dr im1 1Dr im1 7Dr im1 8
Dr im1 9Dr im2 3
Dr im1 0Dr im1 2
A29 1Cho ndA29 2Cho nd
A29 3
COZ82Dr im1
Dr im4Dr im1 3
Dr im1 5Dr im2 0
Dr im2 1Dr im2 4Dr im2 5
AF0 9074 8CHAoo sAF5 3376 1ChMo raca
Dr im1 6A13 6Cho nd
A13 7Cho ndA14 7A54 5
A41 9Cho ndA54 3Cho nd
A29 84A55 0
A29 85A55 1
A55 2A54 6A54 7
A13 5A14 3
A14 4A14 6
A41 7A42 4
A42 5
A42 6A42 7
A45 7A13 8
A14 0A14 1
A41 8A42 0A42 1
A42 2A42 3
A54 4A54 8
Dr im2Dr im6Dr im1 4
Dr im5Dr im9
Dr im2 2A14 5
A14 8A54 2
A13 9A14 2AF0 9074 7Chp respens is
13 54GA30 23
A55 3A55 4
Pr espa2Pr espa113 42G
A54 9AY02 6401 Chcyri
49 0GCHon dPiniosA27 16
A27 19A27 20
A27 23A27 25Var3
Var11Var13
A27 17A27 21
A27 22A27 24
A27 26
Var12Var1
Var2Var6
Var8Var10
A27 18Var4Var5
Var7Var9
Var14G1
M2ST1AST1B
ST1CST1I
ST1JST1E
ST1HM2A
B1 3ST1DST1F
ST1GST1K
ST1LAY02 6400 Chr egium
AF5 3375 6Chr eg iu mAF5 3375 7Chr eg iu m
AF5 3375 4Chr eg iu m
AF5 3375 5Chr eg iu mAF0 9560 6Cho xyr hynchum
AF5 3375 8Chtox ostomaAF5 3376 9Chtox ostoma
AY56 8598 Charr ig onisAY56 8619 Chtur iens is
AY56 8609 Chmieg iiAF0 4598 6Chlus itanicu m
AY56 8607 Chlemming ii
AY58 2741 Chor etanumAY25 4680 Charcas ii
AY25 4683 CholigolepisAY56 8602 Chdu rien sis
AY56 8610 Chpo ly lepisAY56 8617 Chwillk ommi
AY56 8613 Chsalman tinu m0.1
1/84
0.9/-
1/-
0.99/77
1/100
1/82
0.96/-
1/100
1/89
1/88
0.61/-
0.85/85
1/94
Iberochondrostoma, Parachondrostoma
Achondrostoma, Pseudochondrostoma
C. regium
C. kneriiC. soetta
C. phoxinusProtochondrostoma genei
C. nasus
C. holmwoodii
C. meandrense
C. ohridana
C. vardarense
C. prespense
C. cyri
C. oxyrhynchum
Chondrostoma
Albania
Haplotype network of Chondrostoma from Ohrid –
Drim – Skadar and other basins of Albania
Chondrostoma
Distribution of European species of Alburnus alburnus group according to Kottelat and
Freyhof (2007)
Numerous further species, mainly from A. chalcoides species group, in Aegean and
Black Sea baisins in Europe, Near and Middle East and Caspian basin.
A. scoranza
A. alburnus
A. alborella
A. albidus A. belvicaA. thessalicus
A. macedonicus
A. sp. volvi
Additional species available from GenBank: A. hohenackeri, A. macedonicus, A.
thessalicus, A. chalcoides, Alburnus from lower Struma, A. filippii, A. mossulensis, A.
orontis, A. baliki, A. escherichii
Sampling localities of new
material analysed in this study:
A. cf. Alborella
A. neretvae
A. scoranza
A. belvica
A. alburnus
Alburnus from upper Struma =
cf. thessalicus
A. chalcoides
AF090777Tr opspartAY509846Scery th
B84PalepB29Ppseuda
BiH14TrebizatA1999ALBThrA2016AlbThrA2017AlbThrA2018ALBThrA2019ALBThrBiH13TrebizaA1997ALBThrA1998ALBThrA2025AlbThr
Tre2TrebisnjicaTre3TrebisnjicaA1835AlbThrA1836AlbThrA1837AlbThrA1838AlbThrA1839ALBThrA1840ALBThrA1841ALBThrA1842ALBThrA1843AlbThrA1844AlbThrA1846AlbThrA1847AlbThrA1848AlbThrA1849AlbThr
Tre4TrebisnjicaTre1TrebisnjicaA1898AlbThrA1900ALBThrA1914AlbThr
A1845AlbThrA1897AlbThrA1899ALBThrA1915AlbThrA1913AlbThr
A1916AlbThrA1917ALBThrMK1AlbThrMK2AlbThrMK3AlbThrMK4AlbThrIB4AlbThrIB5AlbThr
NC008659AalbSaoneAB239593AalbSaoneY10443AalbIL16ALBThrOLS16AAAlbThr
A1320AlbThrIL15PhoxThrIL17ALBThrB10AAlbThrB14AAlbThrUBL5AAAlbThrB36AALbThrIL18ALBThrIL19ALBThrA1317ALBThrA1318ALBThrST1AAAlbThrB36BALbThrST1BAAlbThr
ST1CAAlbThrOK12CAlbThr
ST1DAAlbThrST1EAAlbThrOK12AAlbThrOK12BAlbThrOLS16BAAlbThrA2395ALBThr
AF090745AstrA1319ALBThrAY026393Aalhohe
AF090743AmacAF090744Athess
AF090746Abelvica1338GALBThr rep1337GALBtHRr epA2930AlbGlu1335GAlbThr1341GAlbThr1332GAlbThr1336GAlbThr1339GAlbThr1340GAlbThr rep
Zet104ZetaZet105Zeta
Zet4ZetaA243PhoxThrA30AlbThrA35AlbThrA37ScALbThrBos762AlbThrA58ScAlbThrBos761AlbThrA125ScALbThrBos763AlbThrA41ScALbThrALS6AlbThrA244AlbThrBos741AlbThr
Als1AlbThrA3PhoxThrA2929PhoxThrA2931ALBThrA2932AlbThrA2933ALBThrA2934AlbThrA2935AlbThrA512ScAlbThrA461ScALbThrA2938ALS4AlbThrBos738AlbThrBos739AlbThrBos740AlbThrA2936AlbThrA2937AlbThrBos764AlbThrA2939AlbThrA126ScALbThr
A31AlbThrA32AlbThrA511ScAlbThrALS5AlbThrA33AlbThrA59ScAlbThrA42ScALbThrA54AlbThr
AY026390AescherichAF095602Afilippi
AY026394ChachalcAY838935ChachalcB10BAlbThr
AY838936AbalikiAY026391Aorontis
0.1
1/94
1/80
1/961/99
1/99
0.99/100
1/100
0.55/61
1/100
0.75/55
1/99
1/97
1/96
1/85
A. scoranza
A. belvica
A. neretvae
A. alburnus
A. chalcoides
A. cf. alborella
A. filippii
A. escherichii
A. baliki, A. orontis
A. hohenackeri, A. macedonicus, A. thessalicus
Alburnus
Conclusions
Chondrostoma:
• populations from Ohrid –Drim–Skadar System and basins of
Albania
- form a separate clade with high haplotype diversity
- do not belong to C. vardarense or C. nasus
- most related to C. prespense
• populations from north Mediterranean related to Asian
species
Alburnus:
• well resolved relationships within studied taxa
• population from Neretva (Croatia) is a new species
• the status of the species from the Aegean Sea basin is
unclear
Acknowledgements
These studies were supported by the research grant of
the National Museum in Prague and SYNTHESYS
projects, conducted in Museo Nacional de Ciencias
Naturales in Madrid.
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