epidemiological and morphoclinical research in a reovirosis outbreak in broilers

Abstracts / Journal of Biotechnology 185S (2014) S37–S125 S97

Immobilized probiotic on the basis ofcarbonized sorbent and sporogenous bacteria

Aida Kistaubayeva Serikovna ∗, Irina SavitskayaStanislavovna, Azhar Zhubanova Akhmetovna,Nuraly Akimbekov Shardarbekovich, MalikaAbdulzhanova Anvarovna, Dina IbraevaHabdulmanatovna

Institute of Biology and Biotechnology Problems,Al-Farabi Kazakh National University, Almaty,Kazakhstan

E-mail address: aida kaz@mail.ru (A.K. Serikovna).

One of the directions of biotechnology and chemistry is receivingcomposite medical preparations and enriched sorbents. These arecomposite materials consisting of carbon sorbent and biologicallyactive components. The purpose is to develop a new composi-tion material on the basis of carbonized rice husk sorbent andcells on sporogenous bacteria immobilize. It was expected thatthis material should possess sorption and antimicrobial activityagainst microorganisms-intestinal pathogens. Bacillus cells wereattached to surface of carbonized sorbent for the creation of probio-tic preparations. The immobilized bacteria are more resistant to theinactivating factors of environment and gastrointestinal tract thatprovides their safety during the long storage and at passing throughthe stomach. It is explained by the appearing of microcolonies ofprobiotics on a sorbent in different physical and chemical state andby detoxication properties itself sorbent. High clinical efficiency ofimmobilized probiotics was defined. This material possesses thehigh special activity, because it combines in itself sorption prop-erties of porous carrier and high probiotic activity of microbs thatis the sorbent acquires new functions depending on the propertiesof the cells of microorganism attached. Thus, the immobilized pro-biotic on the basis of high carbonization rice husk and cells offeredof sporogenous bacteria.

http://dx.doi.org/10.1016/j.jbiotec.2014.07.329

Epidemiological and morphoclinical research ina reovirosis outbreak in broilers

Petrec Oana 1,∗, Coman Alina 1, Clep Ramona 2,Ghise Alina 3, Stancu Adrian 4

1 Department of Infectious Diseases, University ofAgricultural Sciences and Veterinary Medicine,Timisoara, Romania2 ANSVSA, Bucuresti, Romania3 Department of Physiology, University ofAgricultural Science and Veterinary Medicine,Timisoara, Romania4 Department of Pathological Anatomy and LegalMedicine, University of Agricultural Sciences andVeterinary Medicine, Timisoara, Romania

E-mail address: oana petrec@yahoo.com (P. Oana).

Avian reovirus produces several diseases with different loca-tions such as malabsorption syndrome, arthritis-tenosynovitisviral respiratory infections, enteric infections and conditions ofimmunosuppression. In a poultry farm of chicken broilers, reportedmortality losses, reduced growth and different symptoms. In farmwere performed epidemiological, clinical and anatomopathologicexamination, and for confirmation were performed RT-PCR. Thecumulative mortality weekly, it appears that the limits werebetween 0.34% and 1.46%, the maximum being in the secondweekly. Clinical signs of malabsorption syndrome were repre-

sented by unevenness of chickens, diarrhea and keeping fluff.Clinical signs of arthritis-tenosynovitis syndrome were representedby difficulty walking, lameness and arthritis of the joints tibio-tarsus and tarso-metatarsal. The malabsorption syndrome werehighlighted following gross lesions: feathers deformed, reducedbone development, necrosis of the femoral head, deformation ofthe coast and enlarged proventriculus and histologic changes: thenecrosis of proventriculare papilla, necrosis of the lenticular glands,peeling of serous membrane with accumulation of edema, catarrhalenteritis, exfoliation of the villi and infiltration with lymphoblastsand small lymphocytes. In the arthritis-tenosynovitis syndromeis marked gross lesions: edema of the tarsal and metatarsal ten-don sheaths, of digital flexors, gastrocnemius tendon rupture andserofibrinous arthritis. The avian reovirus were detected by RT-PCRand real-time RT-PCR.

http://dx.doi.org/10.1016/j.jbiotec.2014.07.330

Berberine cell cycle arrest at the G2/M phase viaPI3K and p38 kinase in human chondrosarcomacell line, HTB94 cells

Seong Hui Eo ∗, Song Ja Kim

Department of Biological Sciences, College of NaturalSciences, Kongju National University, Gongju,Chungnam, Republic of Korea

E-mail address: eoseonghee@kongju.ac.kr (S.H. Eo).

Berberine is clinically important natural isoquinoline alkaloid iso-lated from many medicinal herbs. Berberine has been proven tohave many other pharmacological effects including antimicro-bial, antitumor, anti-inflammation. However, until now the precisemechanism of action is not researched in chondrosarcoma. There-fore we investigated the effect of berberine on proliferation inhuman chondrosarcoma cell line, HTB94 cells. Treatment withberberine inhibited cell proliferation in a dose dependent man-ner as determined by MTT assay. Also, berberine induced G2/Mphase arrest as determined by FACS analysis and decreased proteinsinvolved in G2/M phase progression in HTB94 cells as deter-mined by Western Blot analysis. Moreover, berberine increased theexpression of p53 and p21 in a time- and dose-dependent manneras determined by Western Blot analysis. We confirmed that berber-ine dramatically caused phosphorylation of Akt and p38 kinase.Inhibition of PI3K/Akt and p38 kinase with LY294002 and SB203580decreased berberine-induced p53 and p21 expression and restoredcyclin B1, cdc2 and Cdc25c expression. These findings suggestedthat berberine inhibits cell proliferation by cell cycle arrest throughPI3K/Akt and p38 kinase pathway in human chondrosarcoma.

http://dx.doi.org/10.1016/j.jbiotec.2014.07.331

Fungi from Kazakhstan as a potential source foreicosapentaenoic acid production

Yana Tsurkan 1,∗, Irina Guschina 2, JohnHarwood 2, Alla Goncharova 1, Saltanat Orazova 1,Tatyana Karpenyuk 1

1 Al-Farabi Kazakh National University, Almaty,Kazakhstan2 Cardiff University, Cardiff School of Biosciences,Cardiff, United Kingdom

E-mail address: yanatsurkan@mail.ru (Y. Tsurkan).

Long chain polyunsaturated omega-3 fatty acids (LC-PUFA), partic-ularly eicosapentaenoic acid (C20:5�3, EPA) and docosahexaenoic