environmental solutions -dionex
Post on 15-Nov-2014
126 Views
Preview:
TRANSCRIPT
Environmental Solutions
from
Environmental Solutions from Dionex
22427
SamplesDetection
Data Analysis and Reporting
Extraction Separation
ASE®—Accelerated Solvent Extraction
Choose the ASE that Matches Your Laboratory Productivity Requirements
U.S. EPA SW-846 Method 3545 (A)– Pesticides and herbicides– Semivolatiles and PAHs– PCBs– PCDDs and PCDFs– TPH (DRO)– Explosives
Air samples– PUF filters– Quartz filters– XAD resins– Tenax resins– Charcoal adsorbents
Organotin and organoarsenic compounds
BFRs Ionic materials
Automated Sample Concentration
ASE® with In-Cell Cleanup (ICC) Saves Time and LaborBrominated Flame Retardants in Fish Tissue
Extraction and Sample Prep in One Easy Step
Extracts With and Without In-Cell Cleanup of Fish Tissue Using Alumina, Silica Gel, and Acidic Silica Gel (40% H2SO4)
In-Line Sample PrepWithout ICC
With ASE-ICC
Calibration Check
Salmon, ASE-ICC
In-Line Cleanup in ASE® CellExtraction and Sample Prep in One Easy Step
Automated Perchlorate Sample Preparation
0 5 10 15 20–500
0
3,500 1. Alfalfa—Extraction Only2. Alfalfa—Extraction + Sample Prep
µS
Minutes
12
Sample and Dispersant
Alumina Sorbent and OnGuard® Resin Mixture
Extraction FlowNew
Method
Columns for Environmental AnalysisAcclaim® Columns for
Reversed Phase Separations
IonPac® Columns for Ion Exchange Separations
22427
Typical Anions Found in Drinking Water New AS22 Column
Unique COLUMN
CHEMISTRY
Conceptual Illustration of Surface Modification
0 5 10 13–1.00
7.00
µS
Minutes
1
2
3
45
6
7
8
Peaks: ppm1. Fluoride 5 2. Acetate 203. Chloride 104. Nitrite 155. Bromide 256. Nitrate 257. Phosphate 408. Sulfate 30
AS22 Column Method 300.0 Inorganic Anions Drinking Water
Baseline Resolution with High Salt Samples
0 5 10 14–0.200
0.500 1
2 4
56
7
8
9 10
11
µS
3
0 5 10 14–5.0
45.0
12
3
456 7
8 9 1011
Minutes
µS
Peak ppm1. Fluoride 0.842. Formate 0.033. Chloride 15.64. Nitrite 0.015. Unknown NQ6. Chlorate 0.187. Bromide 0.028. Nitrate 0.899. Carbonate NQ
10. Phosphate 0.2211. Sulfate 20.45
Cations in Drinking Water Sample ASTM Method D6919-03*
48 mM Methanesulfonic Acid
Minutes0 2 4 6 8 10 12
0.10
1.00
1 3µS
2 54 6
30 mM Methanesulfonic Acid
0 5 10 15 20 250.10
1.00
1 3
4
µS2 5 6
*EPA proposed for drinking water and wastewater compliance monitoring
Peaks 1. Lithium 0.002 ppm 2. Sodium 19.73. Ammonium 0.074. Potassium 0.995. Magnesium 7.26. Calcium 18.5
RFIC:Easy method developmentImmediate results
Acclaim Explosives ColumnsA Total Solution for SW-846 Method 8330
1. HMX2. RDX3. 1,3,5-Trinitrobenzene4. 1,3-Dinitrobenzene 5. Nitrobenzene6. Tetryl7. 2,4,6-Trinitrotoluene8. 4-Amino-2,6-dinitrotoluene9. 2-Amino-4,6-dinitrotoluene
10. 2,6-Dinitrotoluene11. 2,4-Dinitrotoluene12. 2-Nitrotoluene13. 4-Nitrotoluene14. 3-Nitrotoluene
12
14
3 4
10 119
12 13
5 7 86
Acclaim Explosives E1
AU
0 18 36279 45Minutes
1 214
3 4
1011
91213
5 7
86
Acclaim Explosives E2
Baseline Resolution!
Haloacetic Acids by IC-MS/MSMethod Being Jointly Development by EPA and Dionex
No Derivatization—No Sample Prep
Peaks
1. Chloroacetic acid` 6 ppm
2. Bromoacetic acid 4 3. Dichloroacetic acid 6 4. Bromochloroacetic acid 4 5. Dibromoacetic acid 2 6. Bromide 2 7. Trichloroacetic acid 2 8. Bromodichloroacetic acid 2 9. Chlorodibromoacetic acid 10
10. Tribromoacetic acid 20Minutes
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 380
4800
Inte
nsity
, cps
1 67
8
9
103
4
5
Direct Injection
2New
Method
Novel LC Separation of Nitrosamines for EPA 8270 Using Acclaim® PA
81,200,000
2.2 11.40
1 2 3
4
5 6 79
ESI Overlay
Minutes
Inte
nsity
0 30
0
220 1
2 3
4 567
89mAu
Accurate Analysis of Thermally Labile Compounds!!
20109a
1. N-nitroso-dimethylamine2. N-nitroso-morpholine 3. N-nitroso-methylethylamine
4. N-nitroso-pyrrolidine 5. N-nitroso-diethylamine 6. N-nitroso-piperidine 7. N-nitroso-dipropylamine 8. N-nitroso-dibutylamine9. N-nitroso-diphenylamine
Determination of Carbonyl-DNPH Derivatives Using Coupled Acclaim® PA and Acclaim® C18 Columns
0 4 8 12 16 20 24 28 32 35
1
2
34
567
8
910
11 1213mAU
Minutes
Couple Two Columns: More platesCouple Different Columns: Improved selectivity
DNPH derivatives of:1. Formaldehyde2. Acetaldehyde3. Acrolein4. Acetone5. Propionaldehyde 6. Crotonaldehyde7. Methacrolein8. Butyraldehyde9. Methyl ethyl ketone
10. Benzaldehyde11. Valeraldehyde12. m-Tolualdehyde13. Hexaldehyde
20112
Acclaim® Surfactant Column Separatesa Wide Variety of Surfactants!
Peaks:1. Chloride2. Bromide3. Nitrate4. Xylene sulfonate5. Laurylpyridinium chloride6. Lauryldimethylbenzyl-NH4Cl7. Triton X-1008. Cetyl betaine9. Decyl sulfate
10. Dodecyl sulfate11. C10–LAS12. C11–LAS13. C12–LAS14. C13–LAS
mV
0 5 10 15 20 25 30 35Minutes
1
23
4
56
10
98
1112
13
14
Awards Winning Systems from Dionex!
ICS-3000
Flexible, dual RFIC™ system
Proportioned and RFIC™
gradient system
Multiple detectors
System controller
Multiple thermal zones
Integrated Automation
Dionex Ion Chromatography Systems
A Family of IC and RFIC™ Systems
ICS-1600
Standard integrated designElectrolytic suppressionLCD front panelColumn heater
ICS-900
Entry-level integrated design
Chemical suppression
ICS-1100
Basic integrated design
Electrolytic suppression
ICS-2100
Dedicated, single RFIC™ systemRFIC™ gradient systemElectrolytic suppressionLCD front panelColumn Heater
Innovative Eluent Generation Technology
Vent
Pump
H2O
[KOH] Current
Flow Rate
K+
ElectrolyteReservoir
K+
KOH
O2
DegasUnit
KOHGeneration
Chamber
Cation Exchange Connector
CR-CTC Cation Trap
Pt Anode
(H2O 2H+ + ½O2 + 2e–)
[ + ]
Pt Cathode(2H2O + 2e– 2OH– + HO2 )
[ – ]
KOH + H2
EluGen® KOH Cartridge
14538-06
InnovativeTechnology
RFIC® Systems Let You Take Advantage of Hydroxide Eluents
0.00.20.40.60.81.01.21.41.61.82.02.2
300.1 317 326 300.1 317 326
Con
cent
ratio
n (µ
g/L)
AS19 (Hydroxide Eluent) AS9-HC (Carbonate Eluent)
ChloriteBromateBromate-PCRChlorateBromide
Lower MDLs Using Hydroxide Eluent!
Carbonate Removal Device (CRD)
Innovation:– Permeable membrane device
reduces carbonate– No reagents required– No mechanical pumps required
Values:– Minimizes interference from
carbon dioxide in samples– Improves sensitivity for trace analysis– Improves peak integration
and quantitation of anionsImproved Sensitivity for Trace Anion Analyses
EluentIn
Sample Volume
CO2 (gas)CO2 (gas)Eluent
Out
Regen In(Base from Suppressor) CO3
2–
(Carbonate)
CO32–
(Carbonate)
Regen OutRegen In
To the Conductivity Cell
Inlet Port
Regen Out(To CR-TC)
From Eluent Out Port of the Suppressor
22280
InnovativeTechnology
Low-Level Perchlorate in Drinking WaterWith and Without Carbonate Removal Device
22685
Column: IonPac® AG20, AS20 2 mmEluent: 0.5 mM NaOH 0–12 min
65 mM 12.1–28 mm, 100 mM28.1–35 min
Eluent Source: EGC NaOH with CR-ATCTemperature: 35 °CFlow Rate: 0.25 mL/minInj. Volume: 2 mLRinse Volume: 1 mL (10 mM NaOH)Concentration: IonPac Cryptand C1 (4 × 35 mm)Detection: ASRS® ULTRA II, 2 mm
AutoSuppression external water mode
Peaks: 1. Perchlorate 0.5 µg/L (ppb)2. Unknown —
Concentration: 0.50 mM
µS
0 5 10 20 3515 25 30
1
0
Minutes
65.00
100.00
A: With CRD
B: Without CRD
1
1
2
2
Improved Sensitivity for Trace Perchlorate Analyses
Method 314.1
5 7.5 100
18,000
0
4
SIM 99Counts
µS
Minutes
Conductivity
SIM 99
SIM 101
Perchlorate EPA Method 332.0 (IC-MS)*
Column: IonPac® AG16, AS16, 2-mm i.d.Suppressor: ASRS® ULTRA, 2 mmEluent: 65 mM KOH (EG40)Flow Rate: 0.30 mL/minInj. Volume: 250 µLDetection: 1. Conductivity
2. MSQ, SIM 99, 35CIO4–
3. MSQ, SIM 101, 37CIO4–
MS Conditions: –ESI, 70 V, 350 °CSample: Groundwater diluted 1/10
Peak: Perchlorate ~ 7–8 μg/L
* Method Developed Jointly by EPA-OGWDW and Dionex
Method 332.0
EPA Perchlorate Method 332.0*IC-MS/MS
* Method Developed Jointly by EPA-OGWDW and Dionex
Minutes
Peak Area = 1.00 x e4 Counts
9830
0
Coun
ts/s
ec
0
150
Coun
ts/s
ec
0
485
Coun
ts/s
ec
Peak Area = 2.04 x e5 Counts
Peak Area = 3.16 x e3 Counts
2 4 6 8 10 12 14
Internal StandardMRM 107.0/89.035Cl18O3
1 µg/L (ppb)
MRM 100.9/84.837Cl16O3
5 ng/L (ppt)
MRM 98.9/82.835Cl16O3
5 ng/L (ppt)
16
IC System: Dionex ICS 2500 with Matrix Diversion and Auxiliary Pump
MS/MS System:MDS Sciex API 2000
Internal Standard:0–18 Perchlorate: 1 µg/L*
Inject Volume:100 µL
Perchlorate Isotope Ratio:10,000/3160 = 3.16
* Prepared from 1 mg/L stock standard (Dionex Corp)
Method 332.0
EPA Method 331.0* LC-MS/MS Perchlorate Analysis Using Ion-Exchange Separation,
Matrix Diversion, and MS/MS DetectionConceptual Illustration of Surface Modification
IonPac® AS21
LC-MS/MS Eluent: 200 mM methyl amineFlow Rate: 0.35 mL/minAnalysis Column:IonPac® AS21, 2 mmDiversion: Eluent divert valveDetector: Triple quadrupole MS/MS
* Method developed jointly by EPA-OGWDW and Dionex
Method 331.0
EPA Method 300.1 (B) for Bromate in Drinking Water
(A) (B)ppm ppm
1. Fluoride 0.092. Formate — 3. Chlorite — 0.0204. Bromate — 0.0055. Chloride 72.7 6. Nitrite 0.0907. Chlorate 0.120 0.270 8. Bromide 0.200 0.4009. Nitrate 3.7
10. Carbonate — 11. Sulfate 58.4
–0.05
0.75
µS
7
8(A) Unspiked
1
2
5
6
9 10, 11
0 5 10 15 20 25 30–0.05
0.75
µS
8
Minutes
7
43
(B) Spiked
1
2
5
6
9 10, 11
New AS19 Column
RFIC:Lower
background Lower MDL
4
2-D IC Is Easy with the ICS-3000 System
DC Module
Eluent Generator
Auto Sampler
Dual PumpAutomationManager
Automated with Chromeleon Software
2-D IC Removes Interfering MatrixAS20 and AS16 Chemistry Combination
A. First DimensionColumn: IonPac® AG20, AS20, 4 mm Matrix: ppm Chloride: 1000Bicarbonate: 1000Sulfate: 1000
B. Second Dimension Column: IonPac AG16, AS16, 2 mmPeak: 1. Perchlorate 0.5 ug/L
0.75
1.35
µS
A. High Salt Matrix
0 10 20 30 400.40
1.00
1
µS
Minutes
B. Resolved Peak
Second DimensionResolves peakEnhances signal
Introducing UltiMate 3000 Systems Standard, Micro, and Semiprep LC
A New Family of Environmental HPLC Systems
New LC Systems
The UltiMate x2 Dual-Gradient LC System
Solvent Rack with 6-Channel Degasser
Unique Dual-Gradient Pump: Two Ternary Pumps in
One Housing Autosampler with Optional Thermostatting
Column Compartment with Up to Two Switching Valves
Variable Wavelength or Photodiode Array Detector
The Ultimate for Environmental Analysis Productivity
New LC Systems
Wavelength Scanning Allows Detection of Different Compounds from One Injection
Peaks: 1. Uracil 0.6752. Naphthalene 1.617
3. Biphenyl 1.8754. Fluorene 2.0925. Anthracene 2.4256. Fluoroanthene 2.792
210 nm
275 nm
0 0.50 1 1.50 2 2.50 3 3.50 4–500
0
3,000
mAU
Minutes
4321
1
2
3
45
6
286 nm
250 nm
Scan WavelengthsImprove
Identification!
Fast LC EPA 604 for Phenols
Peaks 1. Phenol2. 2,4- di-NO2-phenol3. 4- NO2-phenol 4. 2-Cl-phenol5. 2-NO2-phenol6. 2-Me-4,6-diNO2-phenol7. 2,4- di-Me-phenol8. 4-Cl-3-Me-phenol9. 2,4- di-Cl-phenol
10. 2,4,6- tri-Cl-phenol11. Penta-Cl-phenol
0 5 10 15–5
192
mAU
Minutes
3X faster with new Acclaim® Fast LC Columns
top related