co-op in emd serono - merck group€¦ · co-op in emd serono. 2 title of presentation | dd.mm.yyyy...

Lande Fu

Jun 6, 2018

Enter the world of crystallography

CO-OP in EMD Serono

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Before I start...

Try to find a question

I learnt the word crystallography for the first time when I was doing the interview.

Because I have no background on it, I don’t even have questions.

Just read the text book

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In the first few months...

Watch and Learn—wet lab experiment

Set up drops

Freeze crystals

Soak

Data collection

?

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I didn’t try to understand everything at once.

Instead I watch Ariele’s operation closely.

Look at other related experiments on the Internet.

In the first few months...

Watch and Learn—wet lab experiment

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At the same time...

Watch and learn—CCP4 refinemnt

Learn CCP4

Learn COOT

Understand the protein structure

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At the same time...

Watch and learn—CCP4 refinemnt

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Set up drops

Proteins grow in buffer

Soaking

Compound binds the proteins

Freeze cyrstals

Send to X-ray souce

Data colleciton

Get diffraction data

Refinement

ARI

Gryphon

Make the plate automatically Take images of each drops in

schedule.

Scoring the drops

Rock

Imager

First project…

Optimization, setup the automation

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Second project…

Antibodies screening—find the right conditions

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Extra project

Python script for PyMOL

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Extra project

Python script for PyMOL

Learn Python

Learn PymOL

Understand the Antibodies

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Extra project

Python script for PyMOL