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The Coupled Microfluidic Microbial Electrochemical Cell as a Self-powered Biohydrogen Generator
Aref Fadakar, Mohammad Mahdi Mardanpour1, Soheila Yaghmaei2
Department of Chemical and Petroleum Engineering, Sharif University of Technology, Azadi Avenue, P.O. Box 11365-9465, Tehran, Iran
Supplementary Materials
Hydrogen Gas Sensor Calibration
The rate of biohydrogen production in the microfluidic MEC was obtained using the
hydrogen gas sensor-MQ-8 and Arduino Uno R3 kit, which is appropriate for
measuring biohydrogen concentrations (less than 10000 ppm) in presence of other
gases. The hydrogen gas-sensor and the electronic kit are shown in Fig. S1.
1 Corresponding Author’s Tel.: +9821661664301E-mail address: mardanpour@ripi.ir (M. M. Mardanpour)2E-mail address: yaghmaei@sharif.edu (S. Yaghmaei)
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(A) (B)
(C)
Fig. S1. (A) The hydrogen gas sensor-MQ-8; (B) Arduino Uno R3 kit; (C) The experimental setup of microfluidic MXC in conjunction with hydrogen measurement
module.
The hydrogen gas sensor-MQ-8 was connected to the Arduino Uno R3 kit and the
laptop. This sensor was placed in the cathodic compartment of the MEC which has 1
ml volume. The Arduino software recorded the adsorbed hydrogen on the surface of
hydrogen gas sensor-MQ-8. To enhance the sensor precision, it was calibrated using
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the characterized hydrogen concentrations. The accurate concentrations of
hydrogen were injected into the cathodic compartment of the microfluidic MEC by
Hamilton Sample Lock syringe (1001SL, volume 1 ml). The recorded hydrogen
concentration has been drawn versus injected hydrogen concentration as the
calibration curve. The calibration curve is shown in Fig. S2.
0 200 400 600 800 1000 1200050
100150200250300350400450
The concentration of injected hydrogen into the compartment / ppm
Hydrogen
concentra
tion meas
ured by
MQ-8 sens
or / ppm
Fig. S2. The calibration curve of the hydrogen measurement module.
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