african network on mycorrhiza – information about laboratories

African Network on Mycorrhiza – information about laboratories

Information about Mycorrhiza laboratory Name: Plant Biotechnoloy and agrophysiology of symbiosis

Address: Dept of Biology, FST guéliz, BP 549 guéliz Marrakech

Contact person: Ahmed Qaddoury ([email protected]

Lab members:

Name Degree

Ahmed QADDOURY Cherki GHOULAM Khalid CHRAIBI

PES PES PES

Btissam MANDRI Mustapha FAGHIRE Adnane BARGAZ Mohamed Batoul CHERKAOUI DEKKAKI Ayoub KASRATI Mohamed Fouad OUSSOUF

Doctorate Students

Lamia BOUDANGA Mohcine Madini Master students

Field site: Arid and semi-arid areas

Available facilities and personnel:

Strains in collection: Glomus mosseae, G. intrardices and local strains (under identification)

Current projects: PRAD, RS 2011/19

Research topics: Geographical location: Determinants of plants establishment and productivity in arid and semi-arid areas: The Role of symbiotic rhizospheric microorganisms.

Key words: arid and semi-arid areas, water deficiency, poor soil, Arbuscular mycorrhizal fungi, rhizobial symbiosis, Nitrogen assimilation, Phosphorus assimilation, water relations

List of recent papers:

- Faghire M ; Samri S., Meddich A., Baslam M Goicoechea N., Qaddoury A., Positive Effect of arbuscular mycorhizal fungi on Biomass production, nutrient status and water relations and in date palm seedlings under water deficiency. Submitted to Acta Horticulturae.

- Aqqua K., Meddich A., Ocampo JA., Garcıa Romera I., Qaddoury A., Effect of saprophytic fungi on arbuscular mycorrhizal root colonization and seedlings growth in date palm under Greenhouse conditions. Submitted to Acta Horticulturae.

- Aqqua K., Meddich A., Ocampo JA., Garcıa Romera I., Qaddoury A., 2010a. Effect of saprophytic fungi on arbuscular mycorrhizal colonization and growth of date palm. Fourth International Date Palm Conference Abu Dhabi, United Arab Emirates; 15 - 17 March, 2010.

- Aqqua K., Meddich A., Ocampo JA., Garcıa Romera I., Qaddoury A., 2010b. saprophytic fungi affect arbuscular mycorrhizal root colonization and growth of date palm seedlings. 1er International Congress “Mycorhizal Symbiosis”: Ecosystems & Environment in Méditerranéen area (MYCOMED)” Marrakech, October, 11 – 13, 2010

- Baslam M., Faghire M ; Samri S., Meddich A., Goicoechea N., Qaddoury A., 2010a. Effect of arbuscular mycorhizal fungi on water relation and nutrient status in date palm seedlings under water deficiency. 1er International Congress “Mycorhizal Symbiosis”: Ecosystems & Environment in Méditerranéen area (MYCOMED)” Marrakech, October, 11 – 13, 2010

- Baslam M., Faghire M ; Samri S., Meddich A., Goicoechea N., Qaddoury A., 2010b. Effect of arbuscular mycorrhizal fungi on date palm response to water deficit. International Symposium on the Biology of the Palm family, PALM 2010, 5-7 Mai 2010, Montpellier, France.

- Baslam M.; Qaddoury A.; Goicoechea M. N., 2009a. Effect of drought on dry matter partitioning and water status in mycorrhizal and non-mycorrhizal date palm seedlings. Third SMBBM International Congress of Biochemistry, 2009, Marrakech, Morocco.

- Baslam M.; Qaddoury A.; Goicoechea M. N., 2009b. Arbuscular mycorrhizal fungi influence growth and antioxidant production by date palm seedlings under well-watered and drought conditions, 2009, Marrakech, Morocco.

- Baslam M.; Baslam M.; Qaddoury A.; Goicoechea M. N., 2008. Effect of mycorrhization on growth parameters, biomass production and water dynamic in date palm seedlings under water deficit. The fifth international conference on biological sciences (ICBS), 2008; Tanta, Egypt.

- Bouizgaren A., Farissi M., Aberchane L., Barakate M., Ghoulam C. et Al Feddy M.N. 2009. Evaluation agrophysiologique de la tolérance des populations marocaines de la luzerne (Medicago sativa L.) au stress hydrique. Congrès International « Biotechnologie Microbienne au Service di Développement »(MICROBIOD), 02-05 Novembre, 2009, Marrakech, Maroc - Faghire M., Bargaz A., Mandari B., Oufdou K., Drevon T.T. and Ghoulam C. Genotypic variation in nodule enzymes activity for SNF among common bean (Phaseolus vulgaris L.) genotypes grown under salinity constraint.Congrès International « Biotechnologie Microbienne au Service di Développement »(MICROBIOD), 02-05 Novembre, 2009). Marrakech, Morroco - BARGAZ Adnane Drevon J. J., Oufdou K., Faghire M., Mandri B., et Ghoulam C., 2009. Growth and phosphorus distribution in Phaseolus vulgaris-Rhizobium symbiosis under Phosphorus deficiency in hydroaeroponic culture. The third international conference on the valorisation of phosphates and phosphorus compounds COVAPHOS 3 2009 Marrakech - Ghoulam C., BARGAZ A., Mandri B., Faghire M., J., Oufdou K., LLUCH C., Tejera Drevon J. Garcia N. Drevon J.J., Herrera-Cervera J.A., Adaptation de la symbiose légumineuse/rhizobia au deficit en phosphore et impact sur sa biodisponibilité dans le sol. The third international conference on the valorisation of phosphates and phosphorus compounds COVAPHOS 3, 2009 Marrakech - Mandri Btissam Drevon J.J., Plassard C., Peix A., Bargaz A., Faghire M., Ghoulam C., Oufdou K., 2009. Determination and partial purification and determination of phosphatases and phytases enzymes in rhizobia strains isolated from Phaseolus vularis. MICROBIOD 2009 Marrakech - Mandri B., Oufdou K., Faghire M. Bargaz A, Ghoulam C., Valverde A., Igual J.M., Velázquez E, and Peix A. 2009. Genetic diversity of salt-tolerant rhizobial strains nodulating Phaseolus vulgaris in Marrakech Tensif-Al Haouz region (Morocco) MICROBIOD 2009 Marrakech - Ghoulam C., Bargaz A., Mandri B., Oufdou K., Faghire M., LLUCH C., Tejera Garcia N. and Herrera-Cervera J.A. caracterization Agrofisiologica de la simbiosis of common rhizobium-phaseolus en diferents nveles de fosforo. XII Reunion nacional de la Sociedad Espanola de fijacion de nitrogeno 2008 Estoril-Cascais Portugal - BARGAZ Adnane Faghire M., Btissam M., Oufdou K., Drevon J. J., et Ghoulam C., 2008. Effect of Phosphorus deficiency on root-nodule O2 permeability in the Phaseolus vulgaris-Rhizobium tropici symbiosis 13th AABNF Congress 2008 Hammamet, TUNISIE - Mandri Btissam Oufdou K., Drevon J.J., Bargaz A., Faghire M., Ghoulam C., Phosphatase activity in nodules of common bean genotypes inoculated with rhizobia strain isolated from morocco soil. 13th AABNF Congress 2008 Hammamet, TUNISIE

- Mandri B., Drevon J.J., Bargaz A., Faghire M., Ghoulam C., Oufdou K., 2008. Analysis of phosphatase and phytase activities in nodules of common bean inoculated with rhizobia strain Ciat 899 under phosphorus deficiency ISB International Symposium on Biotechnology 2008 Sfax, TUNISIE - Ghoulam C., Bargaz A., Mandri B., Oufdou K., Faghire M., LLUCH C., Tejera Garcia N. and Herrera-Cervera J.A., 2008. Agrophysiological characterization of common bean-rhizobia symbiosis responses to different phosphorus levels. XII Congress of Spanish Society for Biological Nitrogen Fixation, June 1st – 4th, Lisboa, Portugal - Faghire M., Bargaz A., Mandri B., Oufdou K. & Ghoulam C., 2008. Évaluation du Comportement de la Symbiose Haricot (Phaseolus vulgaris)- Rhizobia Sous Stress Salin en Conditions Contrôlées. Deuxième édition du Congrès National sur l’Amélioration de la Production Agricole (APA2), 03-04 Avril, Settat, Maroc. - Bargaz A., Faghire M., Mandri B., Oufdou K., & Ghoulam C., 2008. Evaluation du comportement de la symbiose Haricot (Phaseolus vulgaris)-rhizobia sous déficience en phosphore. Conférence nationale sur l’Amélioration de la Production Agricole. 3-4 Avril, Settat, Maroc - Bargaz A; Faghire, M; Oufdou K; Mandri B; et Ghoulam C., 2008. Assessment of common bean (Phaseolus vulgaris)-Rizobia symbiosis behaviour under field conditions in the Haouz area of Morocco 2008. ISB, May 4th-8th Sfax-Tunisia. - Ghoulam C., Herrera-Cervera J.A., LLUCH C., Bargaz A., Oufdou K., Faghire M., a Agrophysiological caracterisation of Common bean/rhizobia symbiosis tolerance to phosphorus deficiency. Collqgue International sur les biotechnologies: Biotech World 2007 Oran Algerie - Faghire M., BARGAZ A., MANDRI B., OUFDOU K., GHOULAM C., 2007. Évaluation du Comportement de la Symbiose Haricot (Phaseolus vulgaris)- Rhizobia Sous Stress Salin en Conditions Contrôlées La deuxième édition du Congrès National sur l’Amélioration de la Production Agricole 2007 Settat, MAROC - BARGAZ Adnane K. Oufdou, M. Faghire, B. Mandri et C. Ghoulam Étude de l’effet de la déficience en phosphore sur la symbiose Haricot (Phaseolus vulgaris)-rhizobia Conférence internationale sur les biotechnologies microbiennes et leurs Applications 2007 Meknes, MAROC - Mandri B. Ghoulam C. and Oufdou K; Drevon JJ Sélection de certaines symbioses rhizobiennes du haricot tolérante au déficit en P Conférence internationale sur les biotechnologies microbiennes et leurs applications 2007 Mekhnès, MAROC - Faghire M., GHOULAM C., OUFDOU K., 2007. Étude de l’impact de la salinité sur la symbiose Haricot (Phaseolus vulgaris)-Rhizobia. Conférence Internationale sur les Biotechnologies Microbiennes et leurs Applications. 2007 Mekhnès, MAROC - Ghoulam C., Raguibi H., Mesbah H., Mandri B., Oufdou K., Drevon JJ., 2007. Assessment of Common bean/rhizobia symbiosis performance under different soil phosphorus levels. Second International RHIZOSPHERE Conference, August 26-31, Montpellier - Mandri B.; Ghoulam C.; Drevon JJ.; Oufdou, K.; 2007.Effect of phosphorus treatment on interactions between common bean lines and native rhizobia from Moroccan soil. Second International RHIZOSPHERE Conference, August 26-31, Montpellier

Training needs:

- Morphological identification and molecular characterization of mycorrhizal fungi.

Partnership:

DR. García-Romera Inmaculada Consejo Superior de Investigaciones Cientificas (SCIC) Station expérimentale del Zaidin, Granada

Prof. CRISTINA Cruz Laboratoire de biologie végétale Faculté des sciences Université de Lisboa.

BECARD Guillaume Laboratoire Surface Cellulaire et Signalisation chez les Végétaux, UMR 546 CNRS/Univ Paul Sabatier Toulouse

Dr. Antonio C. Barbera University of Catania Faculty of Agriculture Dipartimento di Scienze delle Produzioni Agrarie e Alimentari Catania Italia

Hamdi AGWA University Cafr Cheik, Egypt

Jean Jack Drevon, INRA- Montpelier

INRA de Tunis

Universite de Granada

Office Régional de Mise en Valeur Agricole de Tafilallet (ORMVAT), Errachidia

Dr. Meddich Abdelilah, Directeur des pépinières communales de production des plants de palmier dattier et responsable du programme de réhabilitation de la palmeraie de Marrakech

INRA Marrakech

Information about Mycorrhiza laboratory 2/ Name: Head of Range Ecology Laboratory

Address: Institut des Regions Arides 4119 Medenine - Tunisia

Contact person: Dr. Mohamed NEFFATI

Lab members: Dr. Neffati Mohamed, Hamza Amina, Sbissi Imed

Field site: South of Tunisia

Available facilities and personnel: Laboratory of microbiologie, biochimie, ecophysiology

Strains in collection:

Current projects:

Research topics: Biochemical, molecular and cultivation of desert truffles Ecophysiological studies of many arid zone plants in Tunisia

Geographical location: south of Tunisia

Key words:


Training needs:

Partnership:

Information about Mycorrhiza laboratory Name: Soil fertility laboratory of soil and water sciences group.

Address: Avenue Mushununu Panzi n°10

Contact person: +243994472276, +250782278887

Lab members: Prof Dr Mushagalusa Nachigera, Ir Espoir Bagula, Rehema Chirakaza, Murhwali Kabiona and Mutabesha Kasholero

Field site: Bukavu site: principal site of trials and all south Kivu territories.

Available facilities and personnel: In this time we look for purchase laboratories and personnel which will go in the traning. But in this region we can have a potentiality of getting AMF strains because of forest zone presence

Strains in collection: no strains in collection but in November one research project will be load to identificate the strains in Kivu Ecosystem

Current projects: The releasing of phosphorus in degradated soil on South-Kivu in grain legumes for restoring soil fertility.

Research topics: Geographical location:

- Evaluation de la capacité de mycorrhization des souches locales pour une meilleure assimilation du P chez le manioc, le haricot, le soja et le bananier. (The assessment of local mycorrhiza capacity for uptake P on Cassava, Banana, common beans and soya bean crop)

- Etude comparative de la capacité de mobilisation du P des sols dégradés par la méthode de mycorrhization et de la matière organique chez les légumineuses (cas spécifique du haricot et du soja).

- Etc.

Key words: Mycorrhiza, Phosphorus, Legumes and soil fertility

List of recent papers: In preparation because this laboratory have 4 months.

Training needs: In microbiology laboratory process for obtaining Mycorrhiza and Rhizobia inoculums (exploring mycorrhiza and Rhizobia strains). The obtaining of Mycorrhiza sustrate

Partnership: TSBF-CIAT in CIALCA project and Harvest Plus, INERA, Master program of environnement at African Evangelical University.

Information about Mycorrhiza laboratory Name: khabou wahid

Address: Olive tree Institute

Contact person: [email protected]

Lab members: 4 associated researchers

Field site: Genetic resource and improvement of olive tree



Current projects: Mitigate biotic and abiotic stress by AMF (nursery conditions)

Research topics: biotic and abiotic stress by AMF (nursery conditions)

Geographical location: nursery of Olive Tree Institute (sfax, Tubnisia)

Key words: AMF, Olive tree, biotic and abiotic stress


Training needs: biological and physiological aspect of mycorrhization (symbiosis and fungids/host plant relationship)

Partnership: Tunisian research center

Information about Mycorrhiza laboratory

Name : Unité de Biotechnologie et Amélioration des Plantes (UBAP)

Address : Laboratoire de Physiologie Végétale – Département de Biologie et Ecologie Végétales, Faculté des Sciences B.P. 906, Université d’Antananarivo MADAGASCAR

Contact person : Pr Alice ANDRIANJAKA : [email protected]

Mme Berthe RASOAMAMPIONONA : [email protected]

ou [email protected]

Laboratoire members :

Enseignants : Pr Alice ANDRIANJAKA, Dr Noronirina V. RAKOTOARISOA

et Mme Berthe RASOAMAMPIONONA,

Tchnicien : François Régis RANDRIAMANDRANTO,

Etudiants chercheurs : Jhonny RABEFIRAISANA, Lucien RASOLOFOARIMANANA

Available facilities and personnel : laboratoire de culture in-vitro,

- verrerie,

- distillateur,

- autoclave,

- 2 balances de précision, 1 balance max = 4000 g, 1 balance max = 1260 g,

- 2 stéréomicroscopes et 2 loupes binoculaires,

- étuves,

- réfrigérateurs,

- micropipettes,

- pHmètre,

- hotte à flux laminaire

- nombre des pièces : une pièce pour la hotte, une pièce pour le stockage, une pièce avec paillasses pour la manipulation et l’installation des matériels et de placard de rangement, une chambre froide, une chambre de culture et une pièce pour régrigérateurs.

- une serre commune à tous les enseignants chercheurs du Laboratoire de la Physiologie Végétale

Le personnel est constitué par les membres du laboratoire UBAP et les étudiants chercheurs.

Strains in collection :- 6 isolats de champignons mycorhiziens à arbuscules piégés des racines de plantes de Madagascar,

- 6 communautés mycorrhiziennes issues des racines de plantes aromatiques et médicinales de Madagascar,

- Glomus intraradices. Current projects : Lutte biologique intégrée contre Striga asiatica à Madagascar par la valorisation de la

biodiversité microbienne et de la diversité végétale en semis direct sur couverture végétale.

Research topics : Geographical location :

- Champignons mycorhiziens à arbuscules et systèmes de culture sur tanety des Hautes Terres et des jachères forestières du versant oriental de Madagascar.

- Lutte intégrée contre le Striga asiatica dans le Moyen-Ouest de Madagascar.

Key words : Champignons mycorhiziens à arbuscules, systèmes de culture, tanety, Striga asiatica, plantes aromatiques et médicinales.

List of recent papers :

1) Rabefiraisana H. J., B. Rasoamampionona, A. Andrianjaka, R. Duponnois. 2010. Poster : Evaluation du taux de mycorhization des plantes médicinales et aromatiques à Madagascar. Congrès International : « Symbioses Mycorhiziennes : Ecosystèmes & Environnement en Milieu Méditerranéen (MYCOMED) » Marrakech, Maroc du 11 au 13 octobre 2010.

2) Rasoamampionona B., L. Rabeharisoa, A. Andriajaka, R. Michellon, N. Moussa, T. Razafimbelo, C. Plenchette. avril 2008. Evaluation du potentiel infectieux mycorhizogène du sol en semis direct du dispositif d’Andranomanelatra de l’ONG TAFA Antsirabe. Dans : Terre Malgache Spécial semis direct, Université d’Antananarivo, Ecole Supérieure des Sciences Agronomiques, p 179, pp 69-71.

3) Rasoamampionona B., L. Rabeharisoa, A. Andrianjaka, R. Duponnois, C. Plenchette. mars 2008. Arbuscular Mycorrhizae in Malagasy Cropping Systems. In Biological Agriculture & Horticulture, an International Journal for Sustainable Production Systems. Eds: P J C Harris and R D Hodges. Vol. 25, 399p, pp. 327-337.

4) Rasoamampionona B., L. Rabeharisoa, C. Marol, J. Balesdent. 2003. Poster : Etude isotopique de la biodégradation du carbone et de l’azote végétaux (Paille de riz et rhizodéposition du soja Glycine max) en sol ferrallitique de Madagascar). Contribution à la biomasse microbienne. Mémoires de l’Académie Nationale des Arts, des Lettres et des Sciences. Actes du colloque international « Sol, Environnement et Développement » du centenaire de l’Académie malagasy. Fascicule XLIX ISSN 1729-4169, 318p, pp279-286.

Training needs : isolement, multiplication et classification des champignons mycorhiziens ; relation sol- plantes-microorganismes.

Partnership : - Dr Christian PLENCHETTE Directeur de Recherche INRA, UMR 1210, Biologie et Gestion des Adventices (BGA) F-21000 Dijon, France ;

- Dr Robin DUPONNOIS Directeur de Recherche UMR 040, Laboratoire des Symbioses Tropicales et Méditerranéennes, Campus International de Baillarguet, 34398 Montpellier, France.

Information about Mycorrhiza laboratory Name: Dr Joanna Dames (managing Director of Mycoroot Pty Ltd – inoculum production company)

Address: Department of Biochemistry, Microbiology and Biotechnology, Rhodes University, P.O. Box 7026, Grahamstown, 6140, Eastern Cape, South Africa.

Email: [email protected]

Mycoroot, P.O. Box 7026, Grahamstown North, 6148, Eastern Cape, South Africa

Email: [email protected]

Contact person: Dr Joanna Dames

Lab members: Dr Greer Hawley (Lab Associate); Mr Tshepiso Seobi (PhD Student); Mr Andre Meyer (PhD student); Miss Wendy Sekgota (MSc student); Mr Charles Bender (MSc student); Miss Pamella Ntshakaza (MSc student); Ms Christine Bizabane (MSc Student); Mr Zolani Mkile (PhD Student); Miss Carmen Ridsdale (MSc student); Miss Durga Ray (PhD student); Miss Phumeza Sitole (MSc Student); Miss Veronique Chartier Fritzgerald (BSc Hons student).

Field site: Various sites in South Africa

Available facilities and personnel: Departmental facilities include general equipment, molecular equipment, Confocal Microscopy, FACS, qPCR, Pyrosequencing

Strains in collection: Various AM fungi, Ericoid isolates, Kalaharituber pfeilii

Current projects:

Stimulation of mycorrhizal fungi associated with sunflower

Ericoid mcyorrhizal fungi and potential for inoculation of commercial berry species

The effects of soil yeasts and their exudates on arbuscular mycorrhizal colonisation and plant growth

Survey of mycorrhizal fungi in apple orchard and identification of community structure

Nitrogen and phosphorus impacts on grasses and legumes growing on soil at Lushington in relation to soil microbiology

The Kalahari Truffle and host relationships

Arbuscular mycorrhizal fungi as an indicator of soil health under conservation and conventional agricultural practices

Arbuscular mycorrhizal fungi, glycoproteinaceous glomalin and soil environment

Mycorrhizal fungal interactions with other soil microorganisms

Characterisation and expression of glomalin protein associated with arbuscular mycorrhizal fungi

Investigating the role of mycorrhizal fungi and their associated bacteria in improving the growth and health of citrus.

Research topics: Arbuscular mycorrhizal fungi, Mycorrhizal interactions with other soil microorganisms, Ectomycorrhizal fungi, Ericoid mycorrhizas, Commercial applications

Geographical location: Various sites in South Africa

Key words:


Training needs: Can offer training

Partnership: Sweden, Prof R. Findlay, Agricultural University, Uppsala

Argentina, Dr Sonia Fontenla, Universidad Nacional del Comahue, de Bariloche

South Africa: Department of Agriculture, Agricultural Research Council, Various commercial companies.

Information about Mycorrhiza laboratory Name: Bernard Moyersoen

Address: University of Aberdeen Honorary Research Fellow, 52 r G Thiriart 4000 Liège, Belgium.

Contact person:

Lab members:

Field site: Gran Sabana, SouthernVenezuela.

Available facilities and personnel: Light microscope facilities. Molecular work in collaboration with research institutions.


Current projects: Biodiversity and biogeography of ectomycorrhizal fungi associated with Pakaraimaea dipterocarpacea

Research topics: P dipterocarpacea is an interesting model for phylogeographic studies of tropical EcM fungi. This neotropical endemic tree species is phylogenetically related with the most important EcM tropical tree family in SE Asia: the Dipterocarpaceae. The objective of this study is to describe P. dipterocarpaceae EcM fungal diversity and to test whether there is a specific EcM fungal flora associated with this endemic. Geographical location: Estado Bolívar, Venezuela

Key words: Tropical ectomycorrhizas, Pakaraimaea dipterocarpacea, Dipterocarpaceae, phylogeography.

List of recent papers: Moyersoen. 2006. Pakaraimaea dipterocarpacea is ectomycorrhizal, indicating an ancient Gondwanaland origin for the ectomycorrhizal habit in Dipterocarpaceae. New Phytologist. 172: 753-762. Dickie IA, Moyersoen, B 2008. Towards a global view of ectomycorrhizal ecology. New Phytologist 180(2): 263-265.

Training needs:

Partnership:

Information about Mycorrhiza laboratory Name: Rasheed Adeleke

Address:

School of Environmental Sciences and Development: Microbiology North-West University Private Bag X6001, Potchefstroom, 2520, South Africa Tel: +18 299 2512

Contact person: Dr. Rasheed Adeleke

Lab members: 0

Field site:



Current projects:


Key words:


• Adeleke, R.A., Cloete, E., Bertrand, A., and Khasa, D.P. (2010). Mobilisation of potassium and phosphorus from iron ore by ectomycorrhizal fungi. World J Microb Biot. doi: 10.1007/s11274-010-0372-0

Training needs:

Partnership: Universite Laval, Canada

Information about Mycorrhiza laboratory Name: Alex Valentine

Address: Botany & Zoology Department, Science Faculty, University of Stellenbosch, Private Bag X1, Matieland 7602, Stellenbosch, South Africa

Contact person: Alex Valentine

Lab members: Alex Valentine, Aleysia Kleinert, Peter Mortimer, Marcellous Le Roux, Anathi Magadledla, Onica Makhuwana, Anele Tshotsho

Field site: Western Cape Fynbos

Available facilities and personnel: Plant physiological infra-red gas exchange for photosynthesis and respiration, Proteomics, Metabolomics, Transcriptomic core facilities, Glasshouse and growth chambers.

Strains in collection: None

Current projects:

1) Carbon and nitrogen metabolism in phosphorus deficient nodules of legumes.

a) The regulation of PEPc isoforms in the phosphorus deficient nodules

b) The role malate metabolism in phosphorus deficient nodules

c) The carbon costs of N nutrition via biological nitrogen fixation and mycorrhizal symbiosis in phosphorus deficient legumes

d) Roles of micro-RNA and transcription factors during recovery from phosphorus deficiency in legume nodules

e) Respiratory and photosynthetic carbon costs of nitrogen fixation during P deficiency

2) Diurnal and circadian regulation of nitrogen fixation in the model legume, Medicago truncatula.

a) Diurnal and circadian regulation of nitrogen fixation by metabolic gene expression networks and respiratory carbon costs (In collaboration with Udvardi laboratory at the Noble Foundation, USA)

b) Nodule clock genes controlling circadian rhythms and expression networks in Medicago truncatula (In collaboration with Udvardi laboratory at the Noble Foundation, USA)

3) Phosphorus nutrition of a retro-transposon insertional-mutant of Medicago truncatula, that forms arbuscule-free mycorrhizal roots.

a) Function of high and low affinity P-transporters in roots and nodules during phosphate starvation

b) Carbon costs of P acquisition in arbuscule-free roots and nodules during phosphate starvation

Research topics: Carbon costs associated with dual symbioses with mycorrhizas and nitrogen fixing nodules in legumes

Geographical location: Southern Africa

Key words: Nitrogen fixation, dual symbioses, arbuscular mycorrhiza, carbon costs


Daniels CW, Rautenbach F, Mabusela, WT, Valentine AJ, Marnewick, JL (2011). Comparative antioxidant- capacity and -content of leaves, bulbs, roots, flowers and fruit of Gethyllis multifolia L.Bolus and G. villosa Thunb. species. South African Journal of Botany (in press)

Oziniel Ruzvidzo, Lara Donaldson, Alex Valentine, Chris Gehring (2011) Arabidopsis thaliana Natriuretic Peptide, AtPNP-A, is a systemic regulator of leaf dark respiration signaling via the phloem. Journal of Plant Physiology (in press)

Valentine AJ, Benedito VA, Kang Y (2011) Abiotic stress in legume N2 fixation: from physiology to genomics and beyond. In: Annual Plant Reviews Volume 42: Nitrogen Metabolism in Plants in the Post-genomic Era. C Foyer M Zhao (eds) Wiley-Blackwell (Oxford) pp 207-248.

Caroline L. Ward, Aleycia Kleinert, Katia C. Scortecci, Vagner A. Benedito, Alexander J. Valentine (2011). Phosphorus-deficiency reduces aluminium toxicity by altering uptake and metabolism of root zone carbon dioxide. Journal of Plant Physiology 168 (2011) pp. 459-465

Cloete KJ, Valentine AJ, Botha A. (2010) The soil yeast Cryptococcus laurentii alters the photosynthetic and respiratory carbon, nutrient and water economy of a Mediterranean sclerophyll, Agathosma betulina (Berg.) Pillans. Symbiosis 51 (3): 245-248

Cloete KJ, Przybylowicz WJ, Mesjasz-Przybylowicz J, Barnabas AD, Valentine AJ, Botha A. (2010) Micro-PIXE mapping of elemental distribution in roots of a Mediterranean-type sclerophyll, Agathosma betulina (Berg.) Pillans, colonized by Cryptococcus laurentii. Plant Cell and Environment 33:1005-1015

Valentine AJ (2010) Comment on: “Big Science, little science”. EMBO Reports 11 (3): 152

Cloete KJ, Valentine AJ, Stander MA, Blomerus LM, Botha A (2009). Evidence of Symbiosis between the Soil Yeast Cryptococcus laurentii and a Sclerophyllous Medicinal Shrub, Agathosma betulina (Berg.) Pillans. Microbial Ecology 57 (4):624-632

Gottig N, Garavaglia BS, Daurelio LD, Valentine AJ, Gehring C, Orellano EG, Ottado J (2009) Modulating host homeostasis as a strategy in the plant-pathogen arms race. Communicative and Integrative Biology 2(2): 1-2

Allison l. Gregory, Brenden A. Hurley, Hue T. Tran, Vicki l. Knowles, Alex J. Valentine, Yi-Min She and William C. Plaxton (2009) In vivo regulatory phosphorylation of the phosphoenolpyruvate carboxylase AtPPC1 in phosphate-starved Arabidopsis thaliana. The Biochemical Journal 420 (1):57-65

Mortimer PE, Perez-Fernandez MA, Valentine AJ (2009) NH4+ nutrition affects the photosynthetic and

respiratory C sinks in the dual symbiosis of a mycorrhizal legume. Soil Biology and Biochemistry 41: 2115–2121.

Mortimer PE, Perez-Fernandez MA, Valentine AJ (2008) Photosynthetic and respiratory C costs of N and P nutrition in the dual symbiosis of a mycorrhizal legume. Soil Biology and Biochemistry 40: 1091-1027

Le Roux MR, Kahn S, Valentine AJ (2008) Organic acid accumulation inhibits N2–fixation in P-stressed lupin nodules. New Phytologist 177 (4): 956-964

Gottig N, Garavaglia BS, Daurelio LD, Valentine AJ, Gehring C, Orellano EG, Ottado J (2008) The bacterial pathogen Xanthomonas axonopodis pv. citri uses a plant natriuretic peptide-like protein to modify host responses - a case of molecular mimicry. Proceedings of the National Academy of Sciences of USA 105(47):18631-18636

Le Roux MR, Kahn S, Valentine AJ (2008) Nodular adaptations to P deficiency in amino acid and ureide exporting legumes, lupins and soybeans. Symbioses 48:102-109

Biological Nitrogen Fixation: Towards Poverty Alleviation Through Sustainable Agriculture. Series: Current Plant Science and Biotechnology in Agriculture (2008). Felix D. Dakora, Samson B. M. Chimphango, Alex J. Valentine, Claudine Elmerich, and William E. Newton (Eds). Springer, Volume 42, 2008 ISBN: 978-1-4020-8251-1

Valentine AJ, Kleinert A (2007) Phosphate deficiency affects respiratory metabolism of dark CO2 fixation in mycorrhizal roots. Mycorrhiza 17: 137-143

Cloete KJ, Valentine AJ, Blomerus L, Botha A, Perez-Fernandez (2007) Nutritional effects of indigenous arbuscular mycorrhizal associations on the sclerophyll Agathosma betulina (Berg.) Pillans. Web Ecology 7: 77-86

Valentine AJ (2007) Comment on the multi-person interview: “The future of research universities”. EMBO Reports 8 (12): 1

Le Roux MR, Ward CL, Botha FC and Valentine AJ (2006) The route of pyruvate synthesis under Pi starvation in legume root systems. New Phytologist 169: 399-408

Valentine AJ, Kleinert A (2006) Respiratory metabolism of root-zone CO2 in mycorrhizal plants with NH4+

and NO3– nutrition. Symbiosis Journal Vol. 41, No. 3 (2006), pp. 119-126

Valentine AJ, Mortimer PE, Lintnaar M, Borgo R (2006) Photosynthetic and nutritional responses of drought stressed arbuscular mycorrhizal grapevines. Symbiosis Journal Vol. 41, No. 3 (2006), pp. 127-134

Mortimer PE, Archer E and Valentine AJ (2005) Mycorrhizal C costs and nutritional benefits in developing grapevines. Mycorrhiza 15: 159-165

Meyer A, Valentine A, Botha A, Archer E and Louw K (2005) The occurence and infectivity of arbuscular mycorrhizal fungi in inoculated and uninoculated rhizosphere soils of two-year-old commercial grapevines. South African Journal of Enology and Viticulture 26 (2): 90-94

Van Rooyen M, Valentine AJ and Archer E (2004) The use of arbuscular mycorrhizal inoculants to decrease the transplant shock in young grapevines (Vitis species). South African Journal of Enology and Viticulture 25: nr 2, 37-45

Hengari SN, Valentine AJ and Theron JM (2004) The effect of the colonization extent of vesicular-arbuscular mycorrhizal fungi on the growth of pot grown Pterocarpus angolensis seedlings. South African Forestry Journal 202: 13-20

Meyer A, Valentine A, Botha A, Archer E and Louw K (2004) Young grapevine response and root colonisation following inoculation with arbuscular mycorrhizal fungi. SA Journal of Enology & Viticulture. Vol. 25(1), 26-32.

Viktor A, Cordero-Otero R and Valentine A (2003) Southern African legumes: a sustainable protein source. South African Journal of Science 99 (11/12 ): 1-5

Mortimer P, Jacobs GH, Valentine AJ and Cramer MD (2003) Water relations and photosynthetic response of protea bushes under irrigation. South African Journal of Botany , 69 (2): 135-143

Valentine A, Osborne B and Mitchell D (2002) Form of inorganic nitrogen influences mycorrhizal colonization and photosynthesis of cucumber. Scientia Horticulturae 92, 229-239

Valentine A, Osborne B and Mitchell D (2001) Interactions between phosphorus supply and total nutrient availability on mycorrhizal colonization, growth and photosynthesis of cucumber. Scientia Horticulturae 88, 177-189.

Training needs: Mycorrhizal isolation and identificaton

Partnership: University College Dublin (Ireland), Universidad Pablo de Olavida (Spain), Noble Foundation (USA)

Information about Mycorrhiza laboratory Name: AHLAM HAMIM

Address: 78, BD Med Ben Abdellah, Tanger, Marocco

Contact person: [email protected]

Lab members: Laboratoire d'Ecologie et Environnement (L2E) (Unité Associée au CNRST, URAC32), Faculté des Sciences Semlalia, Université Cadi Ayyad

Field site:

Available facilities and personnel: laboratory, microscope, greenhouses, experimental field, in vitro facilities,


Current projects: Diversity of Arbuscular Mycorrhizal Fungi (AMF) Associated to Potato Crop Under Different Nutriments Regimes in Morocco.

Research topics: Geographical location: North of Morocco

Key words: Potato crop, Mycorrhizal fungi, water stress, olive, identification of mycorrhizal fungi


Training needs: endomycorrhiz in vitro culture, molecular characterizationof mycorrhizal fungi, others techniques….

Partnership: IRD. Laboratoire des Symbioses Tropicales & Méditerranéennes

Information about Mycorrhiza laboratory Name: Mekelle university _ LaRMEP

Address: Mekelle university, regional state of Tigray, P. o. box 390, Mekelle, Ethiopia

Contact person: Emiru Birhane, e-mail- [email protected]

Field site: Dry deciduous woodlands of the Northern and north western lowlands of Ethiopia

Available facilities and personnel: light microscope, trypan blue, chemicals, room, and one professor and a two technicians. Now two are being trained (one to masters levels and the other to a Phd level. At the end of 2011, hopefully the lab will have enough personnel, but no equipment.

Strains in collection: Glomus, but not a pure culture

Projects: none . A running PhD project will be completed in September 2011.

Research topics: identify the diversity of mycorrhizae in the dry woodland forests, improvement of survival and growth of woodland trees through mycorrhizae, water relations

Geographical location:13◦14_ to 13◦42_N, 38◦38_ to 39◦02_E

Key words: dry deciduous woodlands, Mekelle, pulsing

List of recent papers:Emiru Birhane, Thomas W. Kuyper, Frank J. Sterck, Frans Bongers. 2010.

Arbuscular mycorrhizal associations in Boswellia papyrifera (frankincense-tree)dominated dry deciduous woodlands of Northern Ethiopia. Forest Ecology and Management 260 (2010) 2160–2169.

Training needs: Basic skills on identification of mycorrhiza through the classical methods ( microscopic), and molecular techniques.

Partnership: NORAD, VLIR.

Information about Mycorrhiza laboratory Name: CIAT-Tropical Soil Biology Fertility (TSBF)

Address: CIAT-Tropical Soil Biology Fertility (TSBF), United Nations Avenue, off Gigiri Road, P.O Box 823-00621, Nairobi Kenya

Contact person: Joyce Jefwa , e-mail- [email protected]

Field site: Eastern and Central

Available facilities and personnel: Stere- and Compound microscope, Autoclave and ovens, Greenhouse facility, Cold room, facilities for molecular analysis, Soil and plant analysis facilities, clearing and staining reagents, facilities for Glomalin assessment (Spectrophotometer), equipment for hyphal assessment, equipment for measuring root length, centrifuge, blender, sieves, chemicals, room, and one scientist and one technician, one nursery assistant. Three PhD and 2 Msc students.

Strains in collection: 2 Glomus spp. and 1 Gigaspora sp. pure cultures . I lost 9 species from Malawi and 10 from Kenya s ites which were at the National Museums of Kenya (NMK) greenhouse. Species cultural attempts are in progress.

Projects:

1. Exploration of Integrated soil fertility management for banana production and marketing in Uganda and Kenya: Arbuscular Mycorrhizal fungi help establishment and production of tissue culture banana (2004-2007) (Kenya and Uganda)

2. AMF species associated with banana cultivars, banana cultivar evaluation for indigenous AMF performance from nursery up to field establishment (2007-) (Rwanda)

3. Evaluation and scaling up new chemical and biological commercial products for improving and sustaining crop yields in selected agro-ecological zones in sub-Saharan Africa (Kenya, Ethiopia and Nigeria) (2009-

Research topics: Use Arbuscular Mycorrhiza products for nutrient cycling, aggregates and control of pests and pathogens in agroecosystems.

Geographical location: Nairobi

Key words: Soil fertility, crops, biofertilizer


Jefwa J, Vanlauwe B, Coyne D, van Asten P, Gaidashova S, Rurangwa E, Mwashasha M and Elsen A (2010) Benefits and potential use of Arbuscular Mycorrhizal Fungi (AMF) in banana and plantain (Musa spp.) systems in Africa Proc. IC. In: Banana and Plantain in Africa. Eds Dubois et al., 2010. Acta Hort. 879: 479-486

Jefwa JM, Ohiokpehai O, Kavoo AI, Wasike VW (2010) Soil microbe mediated zinc uptake in soy bean: Review. African Journal of Food Agriculture Nutrition and Development 10(11) 4340-4349

M.O. Okumu, P.J.A. van Asten, E. Kahangi, S.H. Okech, J.Jefwa. B. Vanlauwe (2011) Production gradients in small holder banana (cv, Giant Cavendish) farms in Central Kenya. Scientia Horticulturae. 127: 475-481

Gaidashova SV., Van Asten P., Jefwa JM., Delvaux B and Declerck S. (2010). Arbuscular mycorrhizal fungi in East African Highland banana cropping systems as related to edapho-climatic conditions and management practices: case study of Rwanda. Fungal Ecology 3: 225-233.

Joyce Jefwa, Joseph Mungatu, Peter Okoth, Edward Muya, Henry Roimen, Susan Njuguini (2009). Influence of land use types on occurrence of Arbuscular mycorrhizal fungi in the high altitude regions of Mt Kenya. Journal of Tropical and Subtropical Agroecosystems 11: 277-290.

Mwangi MW., Jefwa JM., Monda EO and Okoth SA. (2009) Effect of Trichorderma hirzianum and arburscular mycorrhizal fungi on growth in tomato (Lycopersicum esculanta Mills.) seedlings and Napier (Pennisitum purpureum L.) and Tea (Camellia sinensis L) cuttings. Journal of Tropical and Subtropical Agroecosystems 11: 423-429.

Training needs: Methods of storage and spore and inoculum production

Partnership: ISAR, IITA, Ethiopian Agricultural Research Institute (EARI), Moi University, Bioversity

Information about Mycorrhiza laboratory Name: Equipe rhizogenèse (IRD/Université Montpellier 2)

Address: LCM, Dakar, Sénégal / UMR DIADE, IRD, 911 Avenue Agropolis, 34394 Montpellier cedex 5, France

Contact person: Laurent LAPLAZE

Lab members: Antony Champion, Sergio Svistoonoff, Alexandre Tromas, Didier Bogusz, Claudine Franche, Valérie Hocher

Field site:

Available facilities and personnel: Molecular and cell biology facilities, genomic tools in Casuarina (genechip, EST database, …), hairy root system to maintain AM fungi collections

Strains in collection: Glomus intraradices, Pisolithus albus

Current projects: Comparison of symbiotic signalling in AM and actinorhizal symbioses, genomics of AM and ECM symbioses in Casuarina

Research topics:

Geographical location:

Key words: genomics, Casuarina, signalling, infection


Perrine-Walker F., Gherbi H., Imanishi L., Hocher V., Ghodhbane-Gtari F., Lavenus J., Benabdoun F.M., Nambiar-Veetil M., Svistoonoff S., and Laplaze L. 2011. Symbiotic signaling in actinorhizal symbioses. Current Protein and Peptide Science, in press.

Diagne N, EscouteJ, Lartaud M, Verdeil JL, Franche C, Kane A, Bogusz D, Diouf D, Duponnois R, Svistoonoff S. 2011. Uvitex2B: a rapid and efficient stain for detection of arbuscular mycorrhizal fungi within plant roots. Mycorrhiza. in press

Svistoonoff, S., Sy, M. O., Diagne, N., Barker, D. G., Bogusz, D., Franche, C. 2010. Infection-specific activation of the Medicago truncatula Enod11 early nodulin gene promoter during actinorhizal root nodulation. Mol. Plant-Microbe Interact. (2010). 23 (6): 740-747.

Gherbi, H., Markmann, K., Svistoonoff, S., Estevan, J., Autran, D., Giczey, G., Auguy, F., Péret, B., Laplaze, L., Franche, C., Parniske, M., and Bogusz, D. 2008. SymRK defines a common genetic basis for plant root endosymbioses with AM fungi, rhizobia and Frankia bacteria. Proc. Natl. Acad. Sci. USA., 105: 4928-4932.

Training needs:

Partnership:

Information about Mycorrhiza laboratory Name: Fungal Ecology lab; Institute of Ecology and Earth Sciences, Tartu University

Address: 40 Lai Street; 51005 Tartu, Estonia

Contact person: Leho Tedersoo ; [email protected]

Lab members: Prof. Urmas Kõljalg, Dr. Leho Tedersoo, Dr. Kadri Põldmaa, Dr. Triin Naadel, 3 PhD students: Mohammad Bahram, Teele Jairus, Kessy Abarenkov

Field site: Benin, Cameroon, Gabon, Madagascar, Sao Tome & Principe, Seychelles, Zambia

Available facilities and personnel: Sufficient

Strains in collection: _

Current projects: Biodiversity of ectomycorrhizal fungi and other microbes related to these fungi


Key words: biodiversity, biogeography, molecular ecology, ectomycorrhizal fungi


Tedersoo L, Suvi T, Beaver K, Kõljalg U. 2007. Ectomycorrhizal fungi of the Seychelles: diversity patterns and host shifts from the native Vateriopsis seychellarum (Dipterocarpaceae) and Intsia bijuga (Caesalpiniaceae) to the introduced Eucalyptus robusta (Myrtaceae), but not Pinus caribea (Pinaceae). New Phytol. 175: 321-333.

Tedersoo L, Suvi T, Beaver K, Saar I. 2007. Ectomycorrhizas of Coltricia and Coltriciella (Hymenochaetales, Basidiomycota) on Caesalpiniaceae, Dipterocarpaceae and Myrtaceae in Seychelles. Mycol. Progr. 6: 101-107.

Suvi T, Tedersoo L, Abarenkov K, Gerlach J, Beaver K, Kõljalg U. 2010. Mycorrhizal symbionts of Pisonia grandis and P. sechellarum in Seychelles: identification of mycorrhizal fungi and description of new Tomentella species. Mycologia 102: 522-533.

Norghauer JM, Newbery DM, Tedersoo L, Chuyong GB. 2010. Do fungal pathogens drive diversity-dependent mortality in established seedlings of two dominant African rain-forest trees? J. Trop. Ecol. 26: 1-9.

Tedersoo L, Nara K. 2010. General latitudinal gradient of biodiversity is reversed in ectomycorrhizal fungi. New Phytol. 185: 351-354.

Tedersoo L, Nilsson RH, Abarenkov K, Jairus T, Sadam A, Saar I, Bahram M, Bechem E, Chuyong G, Kõljalg U. 2010. 454 Pyrosequencing and Sanger sequencing of tropical mycorrhizal fungi provide similar results but reveal substantial methodological biases. New Phytol. 188: 291-301.

[2 articles covering all sites are pending review procedure in New Phytol. and Mol. Ecol.]

Training needs: We can PROVIDE training to African students in our lab.

Partnership: colleagues in all countries

Information about Mycorrhiza laboratory Name: Root Symbiosis Unit

Address: Charia Omar Ibn Khattab, BP 763, Rabat, Morocco

Contact person: Bakkali Yakhlef Salaheddine

Lab members: Abourouh Mohemed, Abbas Younes, Kerdouh Benaissa

Field site:



Current projects:

Research topics: Geographical location: Natural diversity of mycorrhizal fungi, Morocco

Key words:


Bakkali Yakhlef S., Kerdouh B., Mousain D., Ducousso M., Duponnois R. & Abourouh M. 2009. Phylogenetic diversity of Moroccan Cork oak woodlands fungi. Biotechnol. Agron. Soc. Environ. 13(4), 521-528.

Belkouri A., Bakkali Yakhlef S., Es-sgaouri A., Aouadj R et Abourouh M. 2009. Tolérance au stress hydrique et à la salinité et caractérisation moléculaire des isolats de Pisolithus spp. récoltés sous Eucalyptus au Maroc. Annales de la Recherche Forestière 40 : 3 – 16.

Belkouri A., Bakkali Yakhlef S., Es-sgaouri A., Aouadj R. et Abourouh M. 2010. Activite antagoniste et caractérisation moléculaire des isolats de Pisolithus spp. récoltés en forêt de la Maamora (Maroc). IOBC/wprs Bulletin Vol. 57, pp. 97-101.

S. Bakkali Yakhlef, D. Mousain, R. Duponnois, M. Ducousso, A. Belkouri, B. Kerdouh, M. Perrineau and M. Abourouh. 2009. Molecular phylogeny of Pisolithus species from Moroccan forests woodlands. SYMBIOSIS 49: No. 3, pp. 157-162.

S. Bakkali Yakhlef, Y. Abbas, P. Yves, M. Abourouh, M. Perrineau & R. Duponnois. 2011. Effective AMF population in roots of Tetraclinis articulata and Lavandula multifida in Moroccan Tetraclinis woodlands. Mycology: An International Journal of Fungal Biology. (sous presse)

Training needs: DNA sequence analysis, Phylogenetic analysis

Partnership: INRA, CIRAD (LSTM), IRD France

Information about Mycorrhiza laboratory Name: Laboratoire Commun de Microbiologie (LCM UCAD/IRD/ISRA)

Address: Centre de recherche IRD/ISRA, Route des hydrocarbures, Bel air, BP. 1386, Dakar, Sénégal

Contact person: Diégane Diouf

Lab members: Diégane Diouf, Aboubacry Kane, Abdala Diédhiou, Tania Wade, Ibrahima Ndoye, Antony Champion, Laurent Laplaze, Adama Diouf, Samba Sylla, Saliou Fall, Ramatoulaye Samba, Mansour Thiao

Field sites: Ferlo (Senegal), Bassin arachidier (Senegal), Delta du Saloum (Senegal), Forêt du Dja (Cameroun)

Available facilities and personnel: Microbiology, biochemistry, molecular and cell biology facilities, genomic tools, plant physiology

Strains in collection: Glomus intraradices, Glomus aggregatum, Glomus mossae, Glomus fasciculatum, Thelephorales spp., Pisolithus albus, Scleroderma verrucosum, Scleroderma dictyosporum, Scleroderma spp, Scleroderma bermudense; 3 000 strains of rhizobia, Frankia sp.

Current projects: (i) The role of halotolerant mycotrophic grasses (Sporobolus robustus and Leptochloa fusca) in the strategy of regeneration of leguminous trees (Acacia seyal Del. and Prosopis juliflora (Swartz) DC) on salt affected soils in Senegal, (ii) Comparison of symbiotic signalling in AM and actinorhizal symbioses, genomics of AM and ECM symbioses in Casuarina, (iii) Management of ectomycorrhizal symbionts associated to an useful exotic tree specie, Coccoloba uvifera L., to improve reforestation performances in the Niayes area at Senegal, (iv) Diversity and behavior of cowpea rhizobia in agrosystems of West Africa, (v) Functional diversity of ECM symbiosis in a monodominant forest of Gilbertiodendron dewevrei in Cameroon, (vi) Innovative management of Acacia senegal trees to improve resource productivity and gum-arabic production in arid and semi-arid Sub-Saharian Africa. Research topics:

Geographical location:

Key words: Mycorrhiza, Atmospheric nitrogen fixation, Acacia spp., Casuarina, Vigna, Coccoloba, Gilbertiodendron, signalling, infection, salt and drought tolerance, soil fertility

List of recent papers (2011):

Perrine-Walker F., Gherbi H., Imanishi L., Hocher V., Ghodhbane-Gtari F., Lavenus J., Benabdoun F.M., Nambiar-Veetil M., Svistoonoff S., and Laplaze L. (2011). Symbiotic signaling in actinorhizal symbioses. Current Protein and Peptide Science, in press. Diagne N, Escoute J, Lartaud M, Verdeil JL, Franche C, Kane A, Bogusz D, Diouf D, Duponnois R, Svistoonoff S. (2011). Uvitex2B: a rapid and efficient stain for detection of arbuscular mycorrhizal fungi within plant roots. Mycorrhiza. in press Dioumacor Fall, Diegane Diouf, Alzouma M. Zoubeirou, Niokhor Bakhoum, Aliou Faye, Saidou N. Sall (2011). Effect of distance and depth on microbial biomass and mineral nitrogen content under Acacia senegal (L.) Willd. trees. Journal of Environmental Management doi:10.1016/j.jenvman.2011.03.038 (in press) Bâ AM, Duponnois R, Moyersoen B & Diédhiou A. (2011). Ectomycorrhizal symbiosis of tropical African trees. Mycorrhiza DOI: 10.1007/s00572-011-0415-x (in press). Bilgo A, Sangare SK, Thioulouse J, Prin Y, Hien V, Galiana A, Baudoin E, Hafidi M, Bâ AM, Duponnois R (2011). Response of native soil microbial functions to the controlled mycorrhization of an exotic tree legume, Acacia holosericea in a Sahelian ecosystem. Mycorrhiza DOI 10.1007/s00572-011-0390-2 (in press).

Arsene Sanon, Thierry Beguiristain, Aurélie Cébron, Jacques Berthelin, Samba N Sylla, Robin Duponnois (2011). Differences in nutrient availability and mycorrhizal infectivity in soils invaded by an exotic plant negatively influence the development of indigenous Acacia species. Journal of Environmental Management DOI: 10.1016/j.jenvman.2011.01.025 (in press).

Bâ AM, Duponnois R, Diabaté M, Dreyfus B (2011) Les champignons ectomycorhiziens des arbres forestiers en Afrique de l’Ouest : méthodes d’étude, diversité, écologie, utilisation en foresterie et comestibilité. Editions IRD 264 p. Duponnois R, Ouahmane L, Kane A, Thioulouse J, Hafidi M, Boumezzough A, Prin Y, Baudoin E, Galiana A, Dreyfus B (2011). Nurse shrubs increased the early growth of Cupressus seedlings by enhancing belowground mutualism and soil microbial activity. Soil Biology and Biochemistry 43 (10) : 2160-2168.

Dioumacor Fall, Mohamed Ourarhi, Mustapha Missbah El Idrissi, Niokhor Bakhoum, Alzouma Mayaki Zoubeirou, Hanaa Abdelmoumen, Diégane Diouf (2011). The efficiency and competitiveness of three Mesorhizobium sp. strains nodulating Acacia senegal (L.) Willd. under water deficiency conditions in the greenhouse; Symbiosis 54: 87-94.

Training needs:

Partnership:

Information about Mycorrhiza laboratory Name: Associate Professor Dr Gerard M. Mutumba

Address: Maker ere University, College of Natural Sciences, School of Biological Sciences, Department of Biology

Contact person : Dr Gérard M Mutumba

Lab members: Mr Henry Kiryose

Field site: Makerere University Campus

Available facilities and personnel: Sieves,centrifuge,microscopes.

Strains in collection: Not yet made

Current projects: 1.Effect of slope on below-ground biodiversity and abundance of mycorrhiza 2.Effect of canopy on belowground biodiversity

Research topics: Geographical location: Eastern Africa, Uganda

Key words: Abundance, Community composition, land use,Mabira Forest

List of recent papers: See Ecology and Management of Soil Biodiversity In Mabira Forest ,Uganda: An inventory CSM-BGBD Report Number 09-02

Training needs: 1.More equipments

2. Masters and PhD programmes for staff

3.Initiation of Curriculum Review

Partnership: None

Information about Mycorrhiza laboratory Name: Soil Microbiology Laboratory

Address: PO Box 812, Biotechnology Centre, University of Yaoundé I, Yaoundé, Cameroon; E-mail: [email protected]

Contact person: NWAGA Dieudonné

Lab members: NANA WAKAM Louise, BOYOMO ONANA, ADAMOU SOULEYMANOU, NGOUNOU WATAT Claude, YOMBO ONOBIONO

Field sites: multi local sites in central and southern Cameroon

Available facilities and personnel: Laminar flow hood, autoclave, growth chamber, 6 rooms, screen house deep freezer, refrigerators, microscope, lyophilizer, fermentor, oven, shaker. Technician (1) and assistants (2), 3-5 Master students/year, 1-2 PhD students/year

Strains in collection: About 20 strains but more than 60 isolates

Current projects:

1-Production of selected arbuscular mycorrhizal (AM) fungi and training farmers on their use on cocoa and coffee plantations

2-Occurence and functioning of arbuscular mycorrhizal fungi in diverse land use systems associated to maize in southern Cameroon

Research topics: Selecting beneficial (AM fungi, legume nodulating bacteria, P solubilising microorganisms and bio control agents) and their potential for agriculture

Geographical location: Humid forest zone of Cameroon

Key words: Arbuscular mycorrhiza, soil fertility, agriculture, plantation, humid forest zone


-Nwaga D., Hamon S., Djiéto Lordon C. et Engelmann F. 2007. Biotechnologies et maîtrise des intrants agricoles en Afrique centrale. Résumés des communications. Réseau ‘Biotechnologies végétales: amélioration des plantes et sécurité alimentaire (BIOVEG)’, AUF/IRD/Université de Yaoundé I/IRAD. Animation Scientifique Régionale, 17-19 décembre 2007, CRESA Forêt-Bois, Yaoundé, Cameroun, 77 p.

-Jemo M., Nolte C. and Nwaga D. 2007. Biomass production, N and P uptake of Mucuna after bradyrhizobia and arbuscular mycorrhizal fungi inoculation, and P-application on acid soil of southern Cameroon. In Advances in Integrated Soil Fertility Management in Sub Saharan Africa: Challenges and Opportunities. Bationo A., Waswa B. Kihara J. and J. Kimetu (eds.), Springer. pp. 277-281.

-Meppe F., Bilong P. and Nwaga D. 2007. Management of improved fallows for soil fertility enhancement in the western highlands of Cameroon. In Advances in Integrated Soil Fertility Management in Sub Saharan Africa: Challenges and Opportunities. Bationo A., Waswa B. Kihara J. and J. Kimetu (eds.), Springer. pp. 855-863.

-Tchameni N.S., Nana Wakam L., Jemo M., Fokom R., Thé C. and Nwaga D. 2009. Variation in growth and P uptake of maize cultivars colonized by arbuscular mycorrhizas on acid soil of southern Cameroon. Res. J. Agric. Biol. Sci. 4(5): 480-488.

-Fokom R., Nana Wakam L., Tchameni S. Nwaga D. 2010. A. Arbuscular mycorrhizal fungi (AMF) colonisation and rhizobia nodulation of cowpea as affected by flavonoid application. Res. J. Agric. and Biol. Sci. 6 (6): 1015-1021.

-Nwaga D, Jansa J, Abossolo Angue M and Frossard E. 2010. The potential of soil beneficial micro-organisms for slash-and-burn agriculture in the humid forest zone of Sub Saharan Africa. Chap 5. In Soil Biology and Agriculture in the Tropics, Soil Biology 21, P. Dion (ed.), Springer-Verlag, Berlin, Heidelberg. Pp 81-107.

-K.E. Giller, P. Tittonell, M. C. Rufino, M.T. van Wijk, S. Zingore, P. Mapfumo, S. Adjei-Nsiah, M. Herrero, R. Chikowo, M. Corbeels, E. Rowe, F. Baijukya, A. Mwijage, J. Smith, . E. Yeboah, W.J. van de Burg, O.M. Sanogo, M. Misiko, N. de Ridder, S. Karanja, C. Kaizzi, J. K’ungu, , M. Mwale, D. Nwaga, C. Pacini, B. Vanlauwe. 2011. Communicating complexity: Integrated assessment of trade-offs within African farming systems to support development policy. Agricultural Systems, Special issue of “Methods and tools for integrated assessment of sustainability of agricultural systems and land use”.104 (2): 191-203.

-Nwaga D., Tenkouano A., Tomekpe K., Fogain R., Kinfack D. M., Tsané G. and Yombo O. 2011. Multi-functional properties of mycorrhizal fungi for crop production: the case study of banana development and drought tolerance. In Innovations as Key to Green Revolution in Africa: Exploring the Scientific Facts. Bationo A., Waswa B. Okeyo J.M., Maina F. and Kihara J. M. (eds.), Life Sciences, Springer (In Press). ISBN 978-90-481-2541-8.

-Fankem H., Nwaga D., Abba M., Deubel A., Merbach W., and Etoa F-X. 2011. Selecting indigenous P solubilizing bacteria for crop improvement in nutrient deficient acidic soils of southern Cameroon. In Innovations as Key to the Green Revolution in Africa: Exploring the Scientific Facts. Bationo A., Waswa B. Okeyo J.M., Maina F. and Kihara J. M. (eds.), Life Sciences, Springer (In Press). ISBN 978-90-481-2541-8.

Training needs: Identification of species, strains typing, molecular tools for soil ecology

Partnership: ETH Zurich, (Switzerland); University of Laval, Québec (Canada); Alliance for Green Revolution in Africa (AGRA), Nairobi (Kenya); Tropical Soil Biology and Fertility (TSBF) Institute of CIAT, Nairobi, (Kenya), Ministry of Agriculture and Rural Development (MINADER), Cameroon.

Excetutive secretary: Dr. Aboubacry Kane ([email protected]), Dr. Abdala Diédhiou ([email protected]) et Pr. Amadou Bâ ([email protected]; [email protected])

Information about mycorrhiza Laboratory. Name: Mycorrhiza Centre MC

Address: P.O. Box 23250, Kampala, Uganda. Tel: +256772653233, Fax: +256 414 345597, E-mail: [email protected]

Contact person: Opiro Lakuma Kenneth. Tel: +256712653233, E-mail: [email protected]

Lab members: Opiro L.K, Achen G., Onen A.

Field site: Kampala

Available facilities and personnel: No facilities available- newly formed. Personnel – as the lab members.

Strains in collection: Funds

Current project: Biochar and Termite soil

Research topics and Geographical location: Effect of biochar and termite soil on mycorrhiza of Pinus caribaea in Uganda.

Key words: Mycorrhiza, biochar and termite soil.

List of current papers:

1. Collection and identification of fruiting bodies from Pinus caribaea plantations in Uganda. 2. Quantification of mycorrhiza in Pinus caribaea nursery in Uganda 3. Seasonal variation and vertical distribution of mycorrhiza of Pinus caribaea in Uganda. 4. Effects of pH, Nitrogen, Phosphorous and Potassium on mycorrhiza of Pinus caribaea in Uganda.

Training needs: Inoculation of mycorrhiza and identification of fruiting bodies.

Partnership: Farmer groups, In the process of linking up with other research stations. Will soon become a member of International Mycorrhiza Society.

Information about mycorrhiza Laboratory. Name: Laboratoire de Microbiologie des Sols (LMS)

Address: Colline de Badalabougou, Faculté des Sciences et Techniques, Université de Bamako, Mali

Contact person: Ousmane Sacko, Tél : +223 66 81 12 ; +223 76 95 01 17 ; +223 20 77 36 86

E-mail : [email protected] ; [email protected]

Lab members: Inamoud Ibny Yattara, Ousmane SACKO, Fallaye Kanté, Mme Diaby Adjaratou, Bakary Konaté, Aguibou Tall et Mohamed Traoré.

Field site: Serre laboratoire

Available facilities and personnel: Aucune Facilité, personnel du laboratoire

Strains in collection: Néant

Current project: 02 projet soumis mais pas encore financés

Research topics and Geographical location: Biofertilisation des cultures

Key words: Rhizobium, mycorhizes, biofertilisation

List of current papers : Néant

Training needs: Biologie moléculaire des champignons mycorhiziens

Partnership: Opérations paysanes, LCM de Dakar, UCL de Belgique

Information about Mycorrhiza laboratories Name: Prof. Dr. Md. Amin Uddin Mridha

Address: Colleague of Food and Agriculture, Department of Plant Production, King Saud University, P.O. Box. No. 2460, Riyadh, Saudi Arabia

Contact person: : Prof. Dr. Md. Amin Uddin Mridha

Lab members: Dr. Abdulaziz Al-Qarawi , Dr. P. P. Dhar, Mr. Osama Al-Gamdhi

Field site: All over Saudi Arabia.

Available facilities and personnel: We have most of the facilities needed for mycorrhizal study.

Strains in collection: Not yet

Current projects: Role of AMF in Nephka formation and AMF in rangeland

Research topics: Desert truffles, AMF range land

Geographical location: Kingdom of Saudi Arabia

Key words: Desert truffles, AMF range land

List of recent papers (Five last papers) :

a) Dhar, P.P. and M. A. U. Mridha 2012. Arbuscular mycorrhizal associations in different forest tree species of Hazarikhil forest of Chittagong, Bangladesh Journal of Forestry Research, 23 (1): 115-122, Here is the URL of the article. On line version of the journal. http://www.springerlink.com/content/1007-662x/23/1/

b) Dhar, P.P. and M. A. U. Mridha, (2012). Biodiversity of arbuscular mycorrhizal associations in some forest trees of Aagoonia, Bangladesh, The Indian Forester, Vol.138 No. 4:344-348Here is the website http://indianforester.org/current.php?seltitle=The+Indian+Forester&selmonth=4&txtayear=2012

Books: Two books have already been published from Germany after my arrival in KSA, in collaboration with my former students, 2011-2012. Another two under review:

1) The link:http://www.amazon.com/Arsenic-mycorrhizal-interaction-crops-Bangladesh/dp/3846500925, from Germany

2) https://www.lap-publishing.com/catalog/details/store/gb/book/978-3-8465-0087-3/biodiversity-of-arbuscular-mycorrhizal-fungi from Germany.

4) Another book: ROLE OF ARBUSCULAR MYCORRHIZAL FUNGI ON GROWTH AND NUTRIENT UPTAKE OF SOME LEGUMES. Prof. Md. Amin Uddin Mridha Ph.D. DIC (London), Plant Production Department, King Saud University, Riyadh, Saudi Arabia, is being reviewed (Mon, Apr 30, 2012 at 9:58 AM, Vitalie Rotaru <[email protected]>

Training needs: Isolation , identification, molecular study

Partnership: Needed

Information about Mycorrhiza laboratory Name: Center of Study on AM Monoxenics

Address: Université catholique de Louvain Earth and Life Institute Applied Microbiology Laboratory of Mycology Croix du Sud 2 bte L7.05.06 1348 Louvain-la-Neuve Belgium Phone #: +32-10-47-22-26 Fax #: +32-10-45-15-01 Contact person: Dr. Hervé Dupré de Boulois

Lab members: Dr. Stéphane Declerck, Dr. Sylvie Cranenbrouck

Field site: -

Available facilities and personnel: Facilities include greenhouses, culture chambers, molecular equipment, and hoods for in vitro work

Strains in collection: Glomeromycota in vitro collection (GINCO – check at www.mycorrhiza.be)

Current projects:

EU-FP7 VALORAM - Valorizing Andean microbial diversity through sustainable intensification of potato-based farming systems (http://valoram.ucc.ie/)

EU-FP7 Rootopower - Empowering root-targeted strategies to minimize abiotic stress impacts on horticultural crops (http://www.rootopower.eu/)

Various national projects on (1) AMF potential in agriculture/horticulture, (2) AMF bio-control potential, (3) impact of cultural practices and abiotic stresses on AMF, (4) AMF biodiversity, taxonomy and phylogeny, (5) AMF cryopreservation, (6) AMF product development.

Details available at www.mycorrhiza.be

Research topics: arbuscular mycorrhizal fungi in natural and agro-ecosystems, in vitro culture of AMF

Geographical location: Belgium

Key words: arbuscular mycorrhiza fungi, phosphate, biotic and abiotic stresses, biodiversity, taxonomy, phylogeny, in vitro culture, cryopreservation, biocontrol, bioremediation, bioenghancement, biofertilization

List of recent papers: from 2011 on AMF

Sosa-Rodriguez, T., Dupré de Boulois, H., Granet, F., Gaurel, S., Melgarejo, L., Carron, M., & Declerck, S. (2013). In vitro mycorrhization of the rubber tree hevea brasiliensis müll arg. In Vitro Cellular and Developmental Biology - Plant, 1-9.

Koffi, M. C., Vos, C., Draye, X., & Declerck, S. (2012). Effects of Rhizophagus irregularis MUCL 41833 on the reproduction of Radopholus similis in banana plantlets grown under in vitro culture conditions. Mycorrhiza, 1-10.

Lalaymia, I., Cranenbrouck, S., Draye, X., & Declerck, S. (2012). Preservation at ultra-low temperature of in vitro cultured arbuscular mycorrhizal fungi via encapsulation-drying. Fungal Biology, 116(10), 1032-1041.

Zavalloni, C., Vicca, S., Büscher, M., de la Providencia, I. E., Dupré de Boulois, H., Declerck, S., et al., (2012). Exposure to warming and CO 2 enrichment promotes greater above-ground biomass, nitrogen, phosphorus

and arbuscular mycorrhizal colonization in newly established grasslands. Plant and Soil, 359(1-2), 121-136.

Büscher, M., Zavalloni, C., Dupré de Boulois, H., Vicca, S., Van den Berge, J., Declerck, S., et al. (2012). Effects of arbuscular mycorrhizal fungi on grassland productivity are altered by future climate and below-ground resource availability. Environmental and Experimental Botany, 81, 62-71.

Derelle, D., Declerck, S., Genet, P., Dajoz, I., & van Aarle, I. M. (2012). Association of highly and weakly mycorrhizal seedlings can promote the extra- and intraradical development of a common mycorrhizal network. FEMS Microbiology Ecology, 79(1), 251-259.

Gaidashova, S., Nsabimana, A., Karamura, D., van Asten, P., & Declerck, S. (2012). Mycorrhizal colonization of major banana genotypes in six east african environments. Agriculture, Ecosystems and Environment, 157, 40-46.

Gallou, A., Declerck, S., & Cranenbrouck, S. (2012). Transcriptional regulation of defence genes and involvement of the WRKY transcription factor in arbuscular mycorrhizal potato root colonization. Functional and Integrative Genomics, 12(1), 183-198.

Gallou, A., Lucero Mosquera, H. P., Cranenbrouck, S., Suárez, J. P., & Declerck, S. (2011). Mycorrhiza induced resistance in potato plantlets challenged by Phytophthora infestans. Physiological and Molecular Plant Pathology, 76(1), 20-26.

IJdo, M., Cranenbrouck, S., & Declerck, S. (2011). Methods for large-scale production of AM fungi: Past, present, and future. Mycorrhiza, 21(1), 1-16.

Zocco, D., Van Aarle, I. M., Oger, E., Lanfranco, L., & Declerck, S. (2011). Fenpropimorph and fenhexamid impact phosphorus translocation by arbuscular mycorrhizal fungi. Mycorrhiza, 21(5), 363-374.

Cardenas-Flores, A., Cranenbrouck, S., Draye, X., Guillet, A., Govaerts, B., & Declerck, S. (2011). The sterol biosynthesis inhibitor molecule fenhexamid impacts the vegetative compatibility of Glomus clarum. Mycorrhiza, 21(5), 443-449.

De Jaeger, N., de la Providencia, I. E., Dupré de Boulois, H., & Declerck, S. (2011). Trichoderma harzianum might impact phosphorus transport by arbuscular mycorrhizal fungi. FEMS Microbiology Ecology, 77(3), 558-567.

De Jaeger, N., de la Providencia, I. E., Rouhier, H., & Declerck, S. (2011). Co-entrapment of Trichoderma harzianum and Glomus sp. within alginate beads: Impact on the arbuscular mycorrhizal fungi life cycle. Journal of Applied Microbiology, 111(1), 125-135.

Training needs: We offer the “International Training on In Vitro Culture of Arbuscular Mycorrhizal Fungi” (check dates: www.mycorrhiza.be) Partnership: Several collaborations in Europe, Africa, and the Americas

Information about Mycorrhiza laboratories Name: Sonia Labidi Address: Laboratoire des Sciences Horticoles (LSH). Institut National Agronomique de Tunisie. 43, Avenue Charles Nicolle 1082-Tunis- Mahrajène Tunisie Contact person: [email protected] Lab members: Sonia Labidi, Fayçal Ben Jeddi, Sana Dhane, Slim Slim Field site: Tunis Available facilities and personnel: one technician and two workers Fungal strains in collection: A mycorrhizal inoculum based on a mixture of five AMF species: Septoglomus constrictum, Funneliformis geosporum, Glomus fuegianum, Rhizophagus irregularis and Glomus sp. Current projects: Project 1 : Contribution des systèmes de cultures intercalaires à la dépollution des sols. Project 2 : Lupin substituant du soja: action symbiotes racinaires autochtones. Projet 3 : Amélioration et valorisation des ressources phytogénétiques fourragères autochtones et leurs symbiotes racinaires dans les systèmes de grandes cultures de la Tunisie septentrionale. Research topics: Effect of abiotic stresses (calcareous, salinity, soil pollution) and mycorrhizal symbiosis on forage plants. Geographical location: Tunisia Key words: Arbuscular Mycorrhizal Fungi, phytoremediation, plant nutrition List of recent papers (Five last papers) : Labidi S., Ben Jeddi F., Tisserant B., Yousfi M., Sanaa M., Dalpé Y., Lounès-Hadj Sahraoui A. 2015. Field application of mycorrhizal bio-inoculants affects the mineral uptake of a forage legume (Hedysarum coronarium L.) on a highly calcareous soil. Mycorrhiza. DOI 10.1007/s00572-014-0609-0. Labidi S., Fontaine J., Laruelle F., Tisserant B., Dalpé Y., Grandmougin-Ferjani A., Douay F., Lounès-Hadj Sahraoui A. 2015. Fly ash-aided phytostabilisation of highly trace element polluted topsoils improves the telluric fungal biomass: A long-term field experiment. In: Applied Soil Ecology. 85: 69-75. Labidi S., Ben Jeddi F., Tisserant B., Debiane D., Rezgui S., Grandmougin-Ferjani A., Lounès-Hadj Sahraoui A.; 2012. Role of arbuscular mycorrhizal symbiosis in root mineral uptake under CaCO3 stress. In: Mycorrhiza. 22. 337-345. Labidi S., Calonne M., Ben Jeddi F., Debiane D., Rezgui S., Laruelle F., Tisserant B., Grandmougin-Ferjani A., Lounès-Hadj Sahraoui A.; 2011. Calcareous impact on arbuscular mycorrhizal fungus development and on lipid peroxidation in monoxenic roots. In: Phytochemistry. 72. 2335-2341. Labidi S., Ben Jeddi F., Dalpé Y., Tisserant B., Grandmougin-Ferjani A., Wallander H., Lounès-Hadj Sahraoui A.; 2010. Identification de quelques espèces de champignons mycorhiziens à arbuscules associés aux plantes de sulla du nord (Hedysarum coronarium L.) au parc National Nahli, Tunisie. In: Revue de l’I.N.A.T. Vol. 25 n° 2. 121-135.

Information about Mycorrhiza laboratories

Name: Hafidi Mohamed Address: Department of Biology Faculty of Science Semlalia Cadi Ayyad University of Marrakesh Morocco Contact person: [email protected] or [email protected] Labmembers: Director of the Laboratory of Ecology and Environment (L2E), Faculty of Science Semlalia Cadi Ayyad University of Marrakesh Field site : Use of mycorhizes in the system soil-plant ; valorization of organic wastes using composting

process. Available facilities and personnel: Laboratories (L2E), Three professors, 01 post doc, 07 PhD Students and 02 Students in Masters. Fungal strains in collection: Five collections of arbuscular mycorrhizal fungal isolates Current projects: Research topics: The use of mycorrhizal fungi and composts selected in the fight against biotic and abiotic stress in the plant ; Soil and environment ; valorization of organic wastes using composting process ; Organic matter analysis and characterization Geographical location: North and south of Morocco Key words: mycorrhiza, compost, rock phosphate, drought, salinity, plant resistance. List of recent papers (Five last papers) : 1. Mohamed Radi, Hanane Hamdali, Abdelilah Meddich, Lahcen Ouahmane, Mohamed Hafidi : The

mycorrhizal potential of urban soils in semi-arid zones and tolerance of date palm (Phoenix dactylifera L.) to water deficit. Journal of Materials and Environmental Science (ISSN : 2028-2508 CODEN: JMESCN) 5 (6) (2014) 1957-1967 (2014).

2. Abdoulaye SOUMARE, Georges Anicet MANGA, Saliou FALL, Mohamed HAFIDI, Ibrahima NDOYE, Robin DUPONNOIS : Effect of Eucalyptus camaldulensis amendment on soil chemical properties, enzymatic activity, acacias growth and roots symbioses. Agroforestry Systems. DOI 10.1007/s10457-014-9744-z (2014).

3. Hafidi, M., Ouahmane, L., Thioulouse, J., Sanguin, H., Boumezzough, A., Prin, Y., Baudoin, E. , Galiana, A. and Duponnois, R. : Managing Mediterranean nurse plants-mediated 1 effects on soil microbial 2 functions to improve rock phosphate solubilization processes and early growth 3 of Cupressus atlantica G. Ecological Engineering 57, 57– 64 (2013).

4. Bilgo A. , Sangare S.K., Thioulouse J. , Prin Y., Hien V. , Cissoko M. , Galiana A., Baudoin E., Bâ A.M. , Hafidi M., Dreyfus B. & Duponnois R. : Response of native soil microbial functions to the controlled mycorrhization of an exotic tree legume, Acacia holosericea in a Sahelian ecosystem. Mycorrhiza 22 (3) , pp. 175-187 (2012).

5. Ouahmane, L., Revel, J.C., Hafidi, M., Thioulouse, J., Prin, Y., Galiana, A. , Dreyfus, B. & Duponnois, R. : Responses of Pinus halepensis growth, microbial soil functionalities and phosphate-solubilizing bacteria after rock phosphate amendment and ectomycorrhizal inoculation. Plant and Soil 320 (1-2), pp. 169-179 (2009).

6. Ouahmane, L., Mohamed Hafidi, Jean Thioulouse, Marc Ducousso, Marija Kisa , Yves Prin , Antoine Galiana BOUMEZZOUGH Ali, and Robin Duponnois: Improvement of Cupressus atlantica Gaussen growth by inoculation with native arbuscular mycorrhizal fungi. Journal of Applied Microbiology, 103, 683-390 (2007).

7. Ouahmane, L., Jean Thioulouse, Mohamed Hafidi, Yves Prin, Antoine Galiana, Christian Plenchette, Marija Kisa, Robin Duponnois : Soil functional diversity and rock phosphate weathering after inoculation with native or allochtonous arbuscular mycorrhizal fungi. Forest Ecology and Management , 241, 200-208 (2007).

8. Duponnois, R. , Ouahmane, L., Galiana, A. , Hafidi, M., Prin, Y. & Dreyfus, B. : Valoriser et conserver les ressources forestières méditerranéennes : rôle potentiel des champignons mycorhiziens associés à la strate arbustive pionnière. Bois et Forêts, N°294(4), 29-37, ISSN 0006-579X (2007).

9. Ouahmane L., Hafidi M., Plenchette C., Kisa M., Boumezouch A., Thioulouse J. & Duponnois R. (2006). Lavendula species as accompanying plants in Cupressus replanting strategies: effect on plant growth, mycorrhizal soil infectivity and soil microbial catabolic diversity. Applied Soil Ecology, 34, 190-199 (2006).

Information about Mycorrhiza laboratories

Name: Meddich Abdelilah Address: Department of Biology Faculty of Science Semlalia Cadi Ayyad University of Marrakesh Morocco Contact person: [email protected] or [email protected] Labmembers: Biotechnologie and Plant Physiology (BPV) and member of Ecology and Environment

Laboratory (L2E), Faculty of Science Semlalia Cadi Ayyad University of Marrakesh, Morocco Field site : The palm groves of Morocco and the underlying cultures Available facilities and personnel: Two laboratories (BPV and L2E), Plastic greenhouse, Shade house, Rooms culture ; Nursery Marrakech for production of date palm (10ha), Three professors, 01 post doc, 06 PhD Students and 02 Students in Masters Fungal strains in collection : Seven collections of arbuscular mycorrhizal fungal isolates Current projects: Protection project of the palm grove of Marrakesh and other projects Research topics : The use of mycorrhizal fungi in the fight against biotic and abiotic stress in plant hosts to significant interest for Morocco Geographical location: North and south of Morocco Key words: mycorrhiza, compost, rock phosphate, drought, salinity, Fusarium oxysporum fsp. albedinis, growth, biochemical and water parameters, tolerance, date palm and the underlying cultures List of recent papers (Five last papers) (Liste des articles récents):

1- Zarik, L., Meddich A. Boumezzough, A., Ouahmane, L., Hijri, M. Duponnois, R., Hafidi,, M. 2014. The role of arbuscular mycorrhizal fungi on drought tolerance of Cupressus atlantica G. to water deficit. Journal Mater. Environ. Sci. 6(Y) (2015).

2- El Faiz, A., Duponnois, R., Winterton, P., Ouhammou, A., Meddich, A., Boularbah, A & Hafidi M. Effect of different amendements on growing of Canna indica L. inoculated with AMF on mining substrate”. International Phyto-remediation Journal (in press, 2015).

3- M. Radi , H. Hamdali , A. Meddich, L. Ouahmane, M. Hafidi. 2014. Le potentiel mycorhizogène des sols urbains en zones semi-arides et la tolérance du Palmier dattier (Phoenix dactylifera L.) au déficit hydrique J. Mater. Environ. Sci. 5 (6) (2014) 1957-1967.

4- Faghire M ; Samri S., Meddich A., Baslam M., Goicoechea N., Qaddoury A., Positive Effect of arbuscular mycorhizal fungi on Biomass production, nutrient status and water relations in date palm seedlings under water deficiency. Acta Hort. (ISHS) 882:833-838 (2010).

5- Zeze A., Brou Y., Meddich A and Marty F. Molecular identification of MIP genes expressed in the roots of an arbuscular mycorrhizal Trifolium alexandrium L. under water stress. Journal of Agricultural Research. ISSN 1991-637X Academic Journals Vol. 3 (1), pp. 078-083, 078-083 (2008).

6- Jaiti F., Meddich A et El hadrami I. Effectiveness of arbuscular mycorrhizal fungi in the protection of date palm (Phoenix dactylifera) against bayoud disease. Journal of Physiological and Molecular Plant Pathology. 71 : 166-173 (2007).

Information about Mycorrhiza laboratory

Name: Laboratoire de Biotechnologies Végétale et Microbienne (LBVM), Département Agriculture et Ressources Animales, Institut National Polytechnique Houphouet Boigny

Address: BP 1093, Yamoussoukro, Côte d’Ivoire

Contact person:

Prof ZEZE Adolphe Tel: +22509774326 E-mail: [email protected]

Lab members:

Prof ZEZE Adolphe Biologie moléculaire Microbiologie des sols-symbiose endomycorhizienne et rhizobium

Dr NANDJUI Jacob Impact de l’endomycorhization sur les nématodes de plantes cultivées

Dr VOKO Bi-Rosin Rodrigue

Ecologie de la symbiose endomycorhizienne

Dr KOUA Dominique Bioinformatique et phylogénies

Field sites:

- Plantain fields in different agroecosytems in Côte d’Ivoire - Cassava fields in different agroecosystems in Côte d’Ivoire - Yam fields in different agroecosystems in Côte d’Ivoire

Student supervision:

Eleven students supervised at Le LBVM.

Thèses de doctorat soutenues Kouadjo Zaka Ghislaine Claude : Etude des mécanismes de résistance et de réduction microbiologiques du chrome : cas de cendres volantes issues d’un site d’enfouissement à Mpunmalanga (Afrique du Sud) soutenue le 12 décembre 2012

Voko Bi Rosin Rodrigue : Agroécologie des communautés de champignons endomycorhiziens dans différentes zones de culture du manioc (Manihot escumenta, Crantz) en Côte d’Ivoire soutenue le 24 janvier 2014

Thèse de doctorat en instance de soutenance Nabdjui Jacob : Ecologie des champignons mycorhiziens à arbuscules associés à la

rhizosphère de l’igname dans trois zones de production en Côte d’Ivoire DEA/Masters

Ouattara Ibrahima : Diversité et abondance de champignons endomycorhizogènes à arbuscules dans différents types de forêts de la TENE (OUME, Côte d’Ivoire), Diplôme d’Etudes Approfondies en Sciences de la nature, Option Biologie et Protection des Végétaux, Université Abobo-Adjamé Zabouo Armand : Diversité et abondance de champignons endomycorhizogènes à arbuscules issus de sols agricoles de la TENE (OUME, Côte d’Ivoire), Diplôme d’Etudes Approfondies en Sciences de la nature, Option Biologie et Protection des Végétaux, Université Abobo-Adjamé

Diplômes d’ingénieurs 1- Fossou Kouakou Romain : Diversité de rhizobium associé à Cajanus cajan à

Yamoussoukro 2- Zako Bi Iritié Marc S : Diversité de champignons mycorhiziens associés au cacaoyer à

Yakro 3- Sery Drolet Jean Marc : Diversité de champignons mycorhiziens associés au manioc à

Bouaflé 4-Amoa Amoa Jésus : Diversité de champignons mycorhiziens associés au bananier

plantain à Abengourou 5-Kouadio Aka Marie-S : Diversité de champignons mycorhiziens associés à l’igname à

Dabakala 6- Coulibaly Seydou : Diversité de champignons endomycorhiziens associés au cacaoyer dans

la région de Soubré

PhD theses underway

1-Kouakou Fossou Romain (Boursier Université de Genève) 2-Amoa Amoa Jésus (Boursier WAAPP/PPAAO) 3-Kouadio Marie-Stéphanie (Boursier WAAPP/PPAAO) 4-Séry Drolet Jean Marc (Boursier WAAPP/PPAAO) 5-Gbongué Louis Raymond (Boursier WAAPP/PPAAO) 6-Fotso Beaulys 7-Doga dabé Masters students 1-Tahou Eric Joel (Boursier WAAPP/PPAAO) 2-Sidibé Daouda (Boursier WAAPP/PPAAO) 3-Loua Dibolou Sandrine (Boursier WAAPP/PPAAO) 4-Niangne née Anoma C. (Boursier WAAPP/PPAAO) 5-Bakayoko Yacouba (Boursier WAAPP/PPAAO) 6-Gnangui Sarah Leatitia (Boursier WAAPP/PPAAO)

02 technicians working full time.

The major equipments are:

- A molecular biology unit (PCR, electrophoresis, Gel imaging, refrigerated centrifuge) - A microbiology unit (Laminar flow, small autoclave, incubators) - A microscopy unit - An autoclave unit - A greenhouse - Two freezers for strains conservation

Current projects:

1- Projet interafricain : African soil microorganisms as a critical resource for agriculture and biotechnology financé par USAID

2- Projet PNUD Génotypage des bactéries symbiotiques de Cajanus cajan en Côte d’Ivoire

3-Projet Ivo-rhize « PPAAO/WAAP “Technologie d’endomycorhization et amélioration de la productivité de l’igname, du bananier et du manioc en Côte d’Ivoire”, Projet Water and sanitation, Royal Society DFID schemes.

Research topics:

1) The use of endomycorrhizal technology to enhance cassava productivity en Côte d’Ivoire

2) Effect of endomycorrhizal inoculation on nematode and Sigatoka damages on plantain 3) Effect of endomycorhizal inoculation on plantain FHB disease 4) The use of endomycorrhizal technology to enhance yam productivity en Côte d’Ivoire

5) Effect of endomycorrhizal fungi on plantain drought resistance 6) Diversity and ecology of rhizobia associated to local leguminous plants 7) Genotyping the rhizobia associated to Cajanus cajan in Côte d’Ivoire


KOUADJO ZGC and ZEZE A 2013. Genes Related to Chromate Toxicity Challenging Systems in Native Staphylococci Species Isolated from a Fly Ash Dumping Site. Geomicrobiology Journal 30, 61–71 • KOUADJO ZGC and ZEZE A 2013. Proteomic analysis of chromate response in Staphylococcus saprophyticus isolated from a fly ash dumping site. African Journal of Biotechnology Vol. 12(18) 2322-2330 • VOKO BI D-R R, NANDJUI J, SERY J-M D, FOTSO B, AMOA JA, KOUADIO MS, COULIBALY S, NIAMKE S and ZEZE A 2013. Abundance and diversity of Arbuscular mycorrhizal fungal (AMF) communities associated with cassava (Manihot esculenta Crantz) rhizosphere in Abengourou, East Côte d’Ivoire. Journal of Ecology and the Environment vol 5 (11) 360-370 • BI VOKO D-R R, S. L. AHONZO-NIAMKE and ZEZE A. 2013. Impact des propriétés physico-chimiques des sols de culture du manioc sur l’abondance et la diversité des communautés de champignons mycorhiziens à arbuscules dans la zone agroécologique d’Azaguié, Sud-Est de la Côte d’Ivoire. Agronomie Africaine 25 (3) : 251 - 264 (2013)

• NANDJUI J, VOKO BI D-R R, KOUADIO MS, FOTSO B, TANO Y and ZEZE A 2013. Assessment of the occurrence and abundance of mycorrhizal fungal communities in soils from yam (Dioscorea spp.) cropping fields in Dabakala, North Côte d’Ivoire. African Journal of Agricultural Research Vol 8(44) 5572-5584 • ZAKO S M I B, TIE B T, ZIRIHI GN, KOUADJO G C Z, FOSSOU R K and ZEZE A 2012. Arbuscular mycorrizal fungi associated with Theobroma cacao L. in the region of Yamoussoukro (Cote d’Ivoire). African Journal of Agricultural Research Vol. 7(6), 993-1001 • FOSSOU R K, KOUASSI N K II, KOUADJO G C Z, ZAKO S M I B et ZEZE A 2012. Diversité de Rhizobia dans un champ cultivé de pois d’angole (Cajanus cajan L.,) (Légumineuses) à Yamoussoukro (Centre Côte d’Ivoire). Agronomie Africaine 24 (1) : 29 - 38

• KOUADJO ZGC and ZEZE A 2012. Native chromium resistant Staphylococci species from a fly ash dumping site in South Africa harbor plasmid pMOL28 determinants. Agronomie Africaine 24 (1) : 7 - 17 • • KOUADJO ZGC and ZEZE A 2011. Chromium tolerance and reduction potential of Staphylococci species isolated from a fly ash dumping site in South Africa. African Journal of Biotechnology Vol. 10(69), 15587-15594

Partnerships:

- Prof Anthony Okoh, University of Fort hare (Afrique du Sud)

-Dr Xavier Perret, Université de Genève (Suisse)

-Prof Peter Golyshin, University of Bangor (Angleterre)

- Dr van Tuinen Diederik, UMR Agroécologie de Dijon (France)

-Prof Don Conwan, University of Pretoria (Afrique du Sud)

-Dr Hervé Sanguin, LSTM de Montpellier (France)

-Prof Mohamed Hijri, Institut de Recherche en Biologie Végétale, Université de Montréal

Information about Mycorrhiza laboratory Name: Laboratoire de biotechnologie des Rhizobia et Amélioration des Plantezs

Address:

Contact person: Pr. Bekki Abdelkader

Lab members: Mme Ighil Hariz Zohra, M. Kerkachi Nouredine, Buazza Khadidja, Nehila Affaf, Derkaoui Nouria et Ben Elhadj Djelloul Saidia

Field site: Mycorhizoremédiation


Strains in collection: Agaricus bisporus, Glomus sp.

Current projects: Tassili, Jeune équuipe JEAI, PNR1,PNR2 et PNR3

Research topics: Geographical location: Oran Algeria

Key words: Mycorhizae, vegetalasation


1- 1IGHILHARIZ Z., 1BENGUESMIA R., 1BENELHADJ DJELLOUL S., 1SEKOUR S., 1MANSOURI M.L. et 1BEKKI AEK : Les symbioses végétales, un atout pour la revégétalisation des sols dégradés : cas des sablières de Terga et Sidi Lakhdar (Algérie).

2- Benguesmia, C. R., Bekki, A., & Duponnois, R. (2009) : Gestion et valorisation des symbiotes telluriques pour une réhabilitation durables des carrières de l’ouest algérien, communication présentée au Congrès international :"Biotechnologie microbienne au service du développement" (MICROBIOD 2009), organisé au Marrakech, MAROC. www.ucam.ac.ma/microbiona

Training needs:

Partnership: LSTM, LCM, INRAA Montpellier,

african network on mycorrhiza – information about laboratories

Documents