M i c r o s c o p y f r o m C a r l Z e i s s

The inverted microscope Primo Vert is focused on the essentials:

Excellent optical quality, ease of use, choice of high quality materials

and an appealing design are all available at an affordable price.

Primo VertAffordable. Easy-to-Use. Ergonomic.

The new fast and convenient solution

for assessing and checking living cells.

Primo Vert addresses both routine and cutting-edge research laboratories which require compact and reliable microscopes for checking cells quickly and efficiently. The microscope can be also used by students in advanced course room settings.

Key customer benefits at a glance:• Excellent price-performance ratio • Easy-to-Use concept: auto on/off function,

universal phase contrast• Long-term mechanical and optical stability • Attractive industrial design

In addition to the binocular tube and trinocular tube (photo) versions, Primo Vert offers an innovation that generates a new class of its own: The Primo Vert Monitor.

Ultimate convenient: Primo Vert Monitor• Monitor allows multi-observation in training situations • Adjustable monitor tilt (45°- 80°)

for ergonomic and comfortable work• Head is no more in a fixed position to the eyepieces;

glasses are no longer a problem• Integrated digital camera, SD memory card slot, USB port• Remote control for the acquisition of images,

remotely or through glass plates of flow boxes

U2OS cells: Magnification 4x Ph0

One example of the numerous applications is molecular oncology

Cancer cells are cultured in incubators for the purposes of research. Their stage of develop-ment can easily be assessed with Primo Vert – a routine step that requires a consistent work-flow and fast results. The microscopic image must provide absolutely reliable information on whether the cells are adhering to the bottom of the vessel, which means they are intact and can be used in further experiments. On the other hand, if they are floating in the nutrient medium, they are stressed or already dead and thus unfit for further use.

U2OS cells: Magnification 40x Ph2

Hela-cella: Magnification 20x Ph2

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Ease of use: Walk away mode and light push-button on the stage – makes a quick check of living cells more than easy.

Increased working distance through easy removal of the condenser, e. g. for use of roller-bottles.

Focused on Essentials – Ease of Use and Reliable Results

Primo Vert presents a number of attractive details to

guarantee quick, easy and reliable handling. This makes Primo Vert

the perfect tissue culture phase contrast microscope.

The walk away mode turns off the light automatically (in addi-tion to manual on/off-function) after 15 minutes – to save energy and prolong lifetime of the bulb.

Modular illumination with halogen light or with longer lasting LEDs. The advantages of LEDs are the stable colour temperature, only one tenth in power consumption, significantly longer lifetime – therefore a lower cost solution.

Special Skin-friendly material coated on all operating elements.

Universal phase slider for all objectives: For ease of use there is one phase ring (Ph 1) available for 10x, 20x and 40x – this means no need to change phase positions when switching magnifications.

Increased working distance through easy removal of the con-denser, e. g. for roller-bottles.

Objective-indicators for fast identification of magnification.

Carrying handle on the back of the scope.

High-quality optics through high-quality glass. Objectives with infinity optics for magnification up to 40x long working distance provide high-contrast and brilliant images.

Swivelling Siedentopf-tubes. The viewing height can be ad-justed to an upper or a lower viewing height according to the preferences of the user. The inter-pupillare distance can be ad-justed down to a minimum of 48mm.

All Primo Vert versions fit well into hoods and flow boxes.

The image acquisition of Primo Vert Monitor can optionally

be operated by remote control.

Primo Vert enables discussion of the microscope image by several users.

The microscope image of the embedded camera can easily be stored on the SD card.

The Primo Vert Monitor – A New Viewing Perspective

With the new Primo Vert Monitor*, Carl Zeiss offers an ideal microscope for

multi-observation purposes. Several users can look at and discuss the microscope

image at the same time without having to adjust any eyepieces individually.

The new “pass by mode” in microscopy. Just walk and check your cell lines in the pass by mode:

• Easy inspection of living cells is possible, i.e. without hav-ing to look through eyepieces.

• Ergonomic quick check of living cells. The monitor can be tilted from 45° up to 80°. So users can easily adjust the LCD monitor to their individual posture.

• Convenient image storage by the push-to-save camera to SD memory card without PC connection. Additionally a computer can be connected to the USB port on the stand.

• No requirement to remove eyeglasses in order to check the microscopic images.

The main applications for Primo Vert are:• Sterility checks • Cell checks before protein, DNA or RNA preparation• Cell checks in the framework of screenings after donation

of substances (e. g. pharmacology) • Differentiation of cell types • Characterisation of cell lines (oncology) • Growing of cells to produce artificial tissues or organs • Documentation of the above test results

*= For research only - not for medical diagnostic.

Primo Vert – Optical and Mechanical Data

Objective change Manual via quadruple objective nosepiece

Objectives Infinity-corrected objective range with W 0.8 mounting threadPlan-Achromat: 4 x / 0.1, 4 x / 0.1 Ph0, 10 x / 0.25 Ph1LD Plan-Achromat: 20 x / 0.3 Ph1, 40 x / 0.5 Ph1, 20 x / 0.3 Ph2, 40 x / 0.5 Ph 2

Phase-Slider Universal phase slider for the objectives Ph1: Convenient and economicalPhase slider for Ph2: Higher resolution

Eyepieces WF-PL 10x/20 Br. foc.

Specimen stageDimensions (width x depth)Specimen guide Verniers with numerical and alphabetic scaleCoaxial drive

Fixed 200 x 239 mmRight sideX direction: Numerical scale, readable from right to left Y direction: Alphabetic scale, readable in the mirror right side

LD condenser 0.3 For magnifications 4 x to 40 x, WD = 72 mm

LD condenser 0.4 For magnifications 4 x to 40 x, WD = 55 mm

Binocular tube 45°/20Interpupillary distanceViewing height

Viewing angle 45°, FOV 20Adjustable from 48 to 75 mm360 to 397 mm

Trinocular (photo)tube 45°/20Photo/video portFixed beam splitting

Viewing angle 45°, FOV 20 Tube factor 1x, 60 mm50 % vis / 50 % doc

Primo Vert Monitor Camera: 5 Megapixel CMOSMonitor size: 8.4”Display: 800 x 600 pixelStorage Medium: Secure Digital (SD) card Outputs/ports: USB 2.0Camera driver for AxioVision LE with special Microscopy software: Configuration tool

Light source HAL: 6 V, 30 WLED: White light, 3 W

• All optics in Primo Vert are anti-fungus treated• Norms and standards met: CE, UL, CSA, IvD, DIN EN 61010-1 (IEC 61010-1), ISO 9001• Available optional accessories: Object guide and several mounting frames for Petri dishes etc., stage in-

serts (metal or glass), stage enlargements, several camera adapters, eyepiece micrometer and eyepiece pointer, neutral density and green interference filters, cameras, AxioVision LE microscope software

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07740 Jena, Germany

BioSciences | Göttingen LocationPhone : +49 551 5060 660Telefax : +49 551 5060 464E-Mail : micro@zeiss.de

www.zeiss.de/micro

M i c r o s c o p y f r o m C a r l Z e i s s

Developed for bio-medical laboratories, for universities and training courses:

Axio Lab.A1 is unmatched when it comes to performance and optics. Highly ergonomic,

easy to operate and brilliant image quality for versatile medical applications.

Particularly in laboratory use, reliable microscopic images

are crucial. With Axio Lab.A1 you are guaranteed to work

with a microscope that combines maximum performance

and cost-effectiveness. The special plus for long-term

microscope users: TUV-approved ergonomics.

Axio Lab.A1 has been designed for daily laboratory ap-

plications. All applications in brightfi eld, darkfi eld, phase

contrast or fl uorescence can be performed readily – with

the full range of ZEISS IC2S objectives.

Axio Lab.A1Ergonomic. Easy-to-Use. Durable.

High reliability for continuous microscopy work.

Key benefi ts at a glance

• TUV-approved ergonomic confi guration for

maximized comfort and a stress-free working position

• Special features for laboratory use, such as

convenient position of all main operating elements and

the use of soft-touch materials

• Excellent optical quality for reliable results

• Long term durability through the use

of high quality materials

• Numerous tubes, including multi-observation and

accessories for a wide range of applications

• Transmitted light illumination alternatively HAL 35 W,

LED daylight or LED warmlight

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TUV*-approved ergonomics: The ergo-version of Axio Lab.A1 .

Comfortable and convenient: Working with supported arm and all main elements within reach of one hand.

Everything at hand: Tool bar on the back of the scope.

The First Laboratory Microscope – With Approved Ergonomics

A number of attractive details are designed to

guarantee the ergonomic and reliable handling

of Axio Lab.A1.

TUV-approved ergonomics. Axio Lab.A1 places strong

emphasis on ergonomic features, because continuous

microscope use is often associated with discomfort, par-

tic ularly in the neck.

With Axio Lab.A1 users are able to examine slides with a

favorable viewing position, keeping the neck and shoulder

muscles relaxed. The height of the microscope is continu-

ously adjustable by 50mm. In addition the tube can be

swiveled from 8°-33° continuously.

IC²S optics, the Infi nity colour-corrected system, is avail-

able only from Carl Zeiss. The system guarantees brilliant,

colour-corrected and high-contrast results, meeting all the

demands placed on image quality.

Guaranteed durability. The more a microscope is used,

the more worthwhile it is to invest in quality. Axio Lab.A1

guarantees long lasting value and a long operating life.

The new multi-discussion system from Carl Zeiss is also

compatible for Axio Lab.A1. It is possible to achieve identi-

cal image orientations for all co-observers. All co-observers

see the same image as the main observer. And a luminous

pointer facilitates the identifi cation of specimen details for

discussion.

Skin-friendly materials enhance comfort. Many parts

of the stand are coated with soft-touch material: These

materials offer superior properties in comparison to metal

surfaces: less cold and higher friction.

Modular illumination. Convenient and easy-to-change

bulbs. The use of the novel warmlight LED in particular

offers the advantages of a long lifetime, energy saving and

halogen-like colour impressions in stained slides.

Integrated tool storage and cable rest in the stand back

fl ap. Tools, e. g. for centring of phase rings are always at

hand and cannot get lost. In addition, the tilted fl ap can

be used as cable rest.

* TUV Rheinland is a world-renowned independent organization which certifi es product safety and ergonomy.

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Hematology: Plasmodium malariae, daisy-head stage in brightfi eld. Specimen: Andrea Michelsen,

Ortenau Klinikum Lahr-Ettenheim, Germany.Hematology:

Blood smear in darkfi eld.Gout inspection: Uric acid

crystals under polarization contrast.

Explore the Possibilities –Axio Lab.A1 Wide Range of Applications

With the help of Axio Lab.A1 diverse diagnostic applications

can be carried out easily and effi ciently.

Axio Lab.A1 is designed for many uses. Microbiology, cy-

tology, hematology and pathology labs as well as those in

school and colleges will benefi t particularly.

Fluorescence microscopy in the lab

Axio Lab.A1 ensures easy-to-use LED-fl uorescence with

2 LED positions and the well known standard push-and-

click-modules from Carl Zeiss. In comparison to standard

HBO illumination the LED fl uorescence is much safer, more

energy effi cient, quicker and easier to use.

Additional advantages: No warm-up and cool-down times

and no need to change or adjust lamps.

The fl uorescent marker FITC is primarily used for antigen-

antibody reactions in the fi eld of immunology. FITC binds

to the antibody molecules and emits an intense green fl uo-

rescence upon excitation with the 470 nm LED.

Brightfi eld and darkfi eld microscopy

In the fi eld of hematology, the usual diagnostic approach

to blood disorders is blood counting and blood fi lm ex-

amination. During blood fi lm examination in brightfi eld

microscopy, the individual types of white blood cells are

counted. Axio Lab.A1 makes this task easier, because the

main operating elements of the microscope, such as the

stage drive, fi ne focus drive and light intensity are all reach

of one hand. This frees up the other hand.

Darkfi eld blood analysis is very useful for the early detection

of serious health conditions, because fi ne and unstained

structures can often not be seen in front of a bright back-

ground. This situation changes if the structures are illu-

minated from the side and viewed in front of the darkest

possible background. The structures then really seem to

light up. Darkfi eld is a useful detection method for minute

details, not easily detectable brightfi eld. It is produced by

indirect sample illumination resulting in bright structures on

a dark image background. Main fi elds of applications are

hematology and dermatology.

Axio Lab.A1 presents a fi ve position Abbe turret condenser

with darkfi eld and Phase contrast 1,2,3.

Polarization contrast is used to detect birefringent struc-

tures, such as crystals or fi bres. In medical microscopy it

helps to detect gout or asbestos.

Mouse kidney after staining with FITC.

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• All optics in Axio Lab.A1 are anti-fungus treated

• Norms and standards met:

CE, CSA(UL), IvD, DIN EN 61010-1 (IEC 61010-1), ISO 9001, ISO 13485

• Available optional accessories: mechanical stages with drives right or left or

right in fi xed position (ergodrive), several types of specimen holders, several

condensers, several binocular tubes, phototubes and ergotubes with differ-

ent adjustment possibilities in respect to height and tilt, multi-observation

equipement, several interference and neutral density fi lters, several illumi-

nation bulbs as HAL or LED, several polarizer and analyzer moduls, several

refl ector modules, several FL-LED modules, several camera adapters, several

microscope cameras and AxioVison microscope software.

Carl Zeiss MicroImaging GmbH07740 Jena, Germany

BioSciences | Göttingen LocationPhone : +49 551 5060 660Telefax : +49 551 5060 464E-Mail : micro@zeiss.de

www.zeiss.de/micro

Axio Lab.A1 – Technical Data

Contrasting techniques Brightfi eld, darkfi eld, phase contrast, simple polarization contrast,

fl uorescence contrast

Illumination Integrated 35 W halogen lamp, 3 W LED (daylight, warmlight)

Objectives

Eyepieces

A-Plan, N-Achroplan, EC-Plan-Neofl uar and all other IC2S objectives

Eyepieces 10 x for fi eld of view of 20 mm or 22 mm

Stand 5-position nosepiece, stage drive right

Ergomomics TUV-approved ergonomics with height-adjustable and tilting tube,

with ergonomic stage and stage drive, with a number of soft-touch

materials to increase working comfort

Co-observation One main-observer with tubes for up to 2 co-observers seated

on the left side of the main observer

M i c r o s c o p y f r o m C a r l Z e i s s

Axio ScopeMore functional. More economical.

The adaptable all-round stand for

your routine and research applications

2

Your Axio Scope

More functional in every component.

More economical in every confi guration.

Axio Scope is your microscope down to

the last detail.

Modularity has always been a prerequisite for customized micro-scope confi gurations. However, modularity has never before been implemented so consistently. The new all-round stand from Carl Zeiss is your microscope in every respect. With its unusual fl exibility, it can be used universally – and is yet indi-vidually tailored to your applications. More economical than ever because you only buy what you need at the moment. And because you have the option of extending your microscope to meet your growing demands. You will be equally impressed by the cost benefi ts and the performance. Axio Scope – the latest generation upright routine microscope.

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Contents

Flexibility 6-7

Transmitted-light Techniques 8-9

Fluorescence 10-11 Fluorescence LED 12-13

Objectives 14

Imaging System 15

Specimen Space 16-17

Operating Comfort 18-19

Axio Scope: System Overviews 20-24

The New Functionality: All the Advantages 25

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5

6

I II III IV V

The upper partsI. For pure transmitted-light applications Equipment: objective turret with 6x BFII. For standard fl uorescence applications Equipment: objective turret with 3x DIC/3x BF, standard interface for HBO 50, HBO 100,

HXP 120, Colibri etc.III. For LED fl uorescence applications Equipment: objective turret with 3x DIC/3x BF, inte-

grated illumination that can accept 4 different LED modules, synchronized switching with the refl ector turret, long operating life, highly economical

IV. For refl ected-light and fl uorescence applications Equipment: objective turret 6x BF/DF, standard inter-

face for HAL 100 or HBO, DF insert in the objective turret, switchable diffuser

V. For refl ected-light and fl uorescence applications Equipment: objective turret 6x DIC/DF, standard inter-

face for HAL 100 or HBO, DF insert in the objective turret, switchable diffuser, accessible aperture and fi eld diaphragm levels, slot for polarizer slider

FlexibilityTwenty-three Stand Versions. Innumerable Interfaces.

An Outstanding Solution for Your Applications and Budget.

An unusual number of stand versions and interfaces results in unusual fl exibility.

This means that you can confi gure your Axio Scope whatever way is useful for you.

Functionally and economically.

New modularityAxio Scope is as individual as the applications that you use it for. You can customize your stand from fi ve upper parts, three lower parts and two vario columns. For simple or more complex tasks. For transmitted-light, refl ected- light or fl uorescence. What is important is that you no longer invest in functions that you don’t even use – a tangible advantage for your budget.

Economical upgradingSimpler and less costly – the modular interface concept of Axio Scope makes future upgrading quite simple. This is economically attractive as you can install many of the components yourself without waiting for expensive ser-vice support.

Versatile range of usesAxio Scope provides a range of uses which are as versa-tile as the tasks in institutes and laboratories. They range from simple routine applications to more complex re-search projects. From anatomy to cytology. From trans-mitted-light applications to multi-fl uorescence labelling. From the thinnest histological section to a specimen 380 mm thick.

BF = Brightfi eld, DF = Darkfi eld, DIC = Differential Interference Contrast

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876

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12

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A B C D E

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The lower partsA. For many routine applications Illumination: LED (Fixed-Koehler), screwed in directly

beneath the condenser carrierB. All standard applications Illumination: 50 W refl ector light, koehlerable beam

path with fi eld diaphragm and aperture diaphragm, fi lter slider and 6-position fi lter wheel

C. For demanding transmitted-light applications with high-intensity illumination

Illumination: 100 W halogen, koehlerable beam path with fi eld diaphragm and aperture diaphragm, fi lter slider and 6-position fi lter wheel

The stand columnsFor examination of thick specimensD. Vario column 380 mm for refl ected-light and

fl uorescence applicationsE. Vario column 560 mm for refl ected-light and

fl uorescence applications

The new variety of interfaces shown here, using the example of upper part III and lower part B. They vary according to the upper and lower parts.

1 Refl ector space2 Tube3 Eyepiece4 Camera adapter5 Camera6 HBO adjustment aid7 Refl ected-light fi lter slider 8 Achromatic illumination adapter9 Refl ected-light source 10 Upper part/lower part11 Spacer12 Lower part/upper part13 Transmitted-light fi lter slider14 Transmitted-light fi lter wheel 15 Condenser carrier16 Condenser17 Modulator disc18 Stage19 Stage carrier20 Objective

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Transmitted-light TechniquesLED or Halogen, Phase Contrast or DIC. Never Before has

a Microscope of This Class Offered so Many Possibilities.

Whether unstained cells, histologically dyed sections or bone samples: transmitted-

light techniques continue to be the standard for many examinations in the clinical

area. The interfaces developed for the Axio Scope are unique. This brings completely

new fl exibility into routine microscopy.

All contrasting techniquesSurprising in this microscope class: the sheer variety of contrasting techniques that you can use for your applica-tions. Axio Scope offers you simply everything. The clas-sical methods of brightfi eld, darkfi eld, phase contrast – but also Differential Interference Contrast, DIC for short. You can also use the economical PlasDIC alternative from Carl Zeiss. Which means that this inexpensive inter-ference contrasting technique is available for the fi rst time with an upright microscope.

Individual confi guration: the transmitted-light beam pathThe transmitted-light beam path of Axio Scope provides impressive image contrast and homogeneous illumination. The great fl exibility in confi guring offered by Axio Scope is new and unique. At the front you have a 6-position fi lter wheel, which comes equipped with the integrated shutter. Individual grey and colour fi lters can easily be added. At the back you can also use an additional fi l-ter slider, which can also be confi gurated fl exibly so that nearly any combination of fi lters is possible. Moreover, if you decide on transmitted-light LED illumination, attenu-ating fi lters are unnecessary. The economical LED illumi-nation can be attenuated as desired without any change in colour temperature – a genuine advantage compared to halogen light.

It’s easy to change to darkfi eld, phase contrast and PlasDIC: the interfaces in the Abbe condenser and modulator disc provide a unique range of contrasting techniques. Simply by inserting the modules.

Versatile options: the LED components (1), the Abbe condenser with/without modulator disc (2), and the modulator disc with exchangeable modules for darkfi eld, phase contrast or PlasDIC techniques (3).

9

LED or halogen? Standard illuminationYou can choose among three subunits optimized for the different geometries of the light sources. 1. Transmitted-light LED: durable, economical and adjustment-free, this illumination provides suffi cient intensity for stained his-tological preparations and other typical routine clinical tasks. 2. 50 W refl ector illumination: this offers more than enough light for all standard procedures such as brightfi eld and phase contrast. 3. 100 W halogen light, ideal for thick specimens and procedures that require a lot of light, such as DIC at high magnifi cations.

Flexible for interference contrast: the Abbe condenserThe possibility of equipping Axio Scope with anything from a very inexpensive bright fi eld condenser to a high-performance interference contrasting technique is new and unique in this class. This is made possible by two unusual interfaces: you simply equip your condenser with the modulator disc for darkfi eld and phase contrast when you need these methods. The modulator disc it-self has exchangeable modules. This allows you to use even PlasDIC, the inexpensive interference contrast from Carl Zeiss, at any time, simply by exchanging the modules.

ISCP: the Infi nity Space Camera PortFor all documentation tasks in transmitted-light applica-tions and only with Axio Scope: the Infi nity Space Camera Port, ISCP for short. This innovative feature offers you an additional option for using the interface in the infi nity space (see also infi nity space interface on pages 10/11). Instead of using this for refl ected-light refl ectors, you can use it for your applications in transmitted-light. That way, you gain a fully functioning camera port, independent of the choice of tube that can be added at any time.

The 6x fi lter wheel comes equipped with an integrated shutter. Grey and colour fi lters can be mounted individually – easily and conveniently since the fi lters are clicked readily into the corresponding holders when the cover is open.

ISCP – the Infi nity Space Camera Port - is simply inserted inthe interface in the infi nity space. For transmitted-light, it isa fully functioning camera port, that can be added any time.

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FluorescenceConfi gured Today. Adapted Tomorrow. The New Fluorescence

Grows With Your Demands.

FluoresScience is the name of the initiative refl ecting Carl Zeiss’ focus on the de-

velopment of excellent fl uorescence techniques. The latest developments of this

initiative refl ect the growing importance of fl uorescence as a routine method.

With outstanding quality, innovative solutions and its ability to be confi gured to meet

requirements down to the smallest detail, Axio Scope sets quite new signals in its class.

Interface for your refl ector inserts: infi nity spaceUnique in this class: the interface in the infi nity space. With Axio Scope you can select the refl ector turret or slider required for your applications. Flexible, simple and quickly added. You have a choice of a 2x slider or a re-fl ector turret with 4 or 6 positions. In addition, Axio Scope offers another feature here as the interface can also be used as a camera port for transmitted-light applications (see Infi nity Space Camera Port, pages 8/9).

Can be retrofi tted to meet your needs:the refl ected-light beam pathAxio Scope is excellently equipped for fl uorescence be-cause the refl ected-light beam path was completely re-calculated to provide outstanding contrast and excellent minimizing of scattered light. On the other hand, you can benefi t from the great fl exibility of the modular system here. The adjustment aid for the HBO can be added – you can simply insert it yourself. The same applies to the achromatic system, which guarantees you even bet-ter results in Multichannel Fluorescence applications.

The interface in the infi nity space allows diverse uses: the 6x and 4x refl ector turret, the Infi nity Space Camera Port (ISCP) and the 2x refl ector slider.

Exchangeable: the standard lens system and the achromatic system for Multichannel Fluorescence applications.

An option that can be added simply: the adjusting aid for setting the HBO burner.

11

Versatile and innovative: the light sourcesThe illumination options of Axio Scope are versatile and simple for the customer to operate. A completely new lamp housing was developed for the HBO 50, whose burner can be removed with the drawer for a particularly simple and convenient change of burner. The HBO 100 comes as a standard or in a self-adjusting version. In ad-dition to HBO illuminators the HXP 120 provides you with a durable, adjustment-free metal halide light source with integrated fast shutter, which can be controlled simply by a hand or foot switch or from AxioVision.

Inserting the refl ector slider, refl ector turret or ISCP in the infi nity space on the vario stand: just one simple movement of your hand.

Simpler than ever: changing the burner in the newly developed HBO 50 lamp housing.

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Fluorescence LEDCompletely New. Completely Integrated. With This Innovative Light

Source Axio Scope Sheds New Light on Routine Work.

A technological development, available for the fi rst time with Axio Scope:

the integrated LED illumination for routine fl uorescence applications. Now

you can have these extremely durable and economical light sources in both

transmitted-light and refl ected-light.

The new LEDThe advantages of LED illumination speak for themselves: extremely long operating life, inexpensive, easy to handle, and completely adjustment-free. And without expen-sive changes of burner for a very long time – instead of operating constantly, the LEDs turn on only when they are used in the beam path. Now Carl Zeiss offers a new integrated LED solution specially designed for routine applications in fl uorescence. The freely mountable LED turret allows you to you use up to 4 LED modules. Syn-chronized with the refl ector turret*, the correct LED is switched on automatically when a new refl ector module is swung in. The intensity can be regulated individually for any colour. Your settings are preserved even when you switch back and forth – an important plus because that way each of your different fl uorescence channels is optimally illuminated at any time.

* Because of the coupling of refl ector turret and LED module turret, the refl ector turret is not exchangeable with this upper part.

Without a tool, easy, and safe: mounting the LED modules on the LED turret. When the cover is opened, a safety switch ensures that the LED is switched off immediately.

13

Full FlexibilityThe fl exible modular concept of Axio Scope leaves you much room for confi guring your stand to meet your needs. The upper part with the integrated LED fl uores-cence (see page 6/7, upper part III) can be combined with all lower parts. If you wish, you simply assemble your complete LED stand with integrated transmitted-light and refl ected-light LED.

Prepared for ColibriAxio Scope is prepared for Colibri, the high-end LED fl uorescence light source from Carl Zeiss. Thus you have a choice to use a light source, which is fully integrated in the AxioVision software. And so you are able to take au-tomatic Multichannel Fluorescence images – on a purely manual stand!

Easily accessible: on/off switching and intensity control of the LED modules.

Colibri for setting up to four wavelengths simultaneously.

14

ObjectivesNew for Routine Work. Tried and Tested for the Highest Demands. Ob-

jectives from Carl Zeiss: the Economical Solution for Every Requirement.

Carl Zeiss stands for more than 150 years for microscope lenses that are

practically unrivalled. And this in all performance classes. Naturally highest

demands on optical performance were also placed on Axio Scope.

Perfect for routine work: the new N-ACHROPLAN objectivesNewly developed and perfect for many of your routine applications: the N-ACHROPLAN series. A very inexpen-sive all-round objective with good chromatic correction and planarity. Moreover, the entire range of objectives from Carl Zeiss are at your disposal. The decision is up to you; from a low magnifi cation overview to high-resolu-tion imaging of details.

Each class is a class of its own – the objectives

• A-Plan – the A class: Good and inexpensive entry-level objectives for

diverse applications; good optical quality for transmitted-light applications

• N-ACHROPLAN – the all-rounders: Inexpensive, versatile and new for many routine ap-

plications in transmitted-light or refl ected-light

• EC Plan-NEOFLUAR – the universal talents: Perfect when fl exibility and a variety of techniques

are required. Based on the IC2S optics, they are characterized by high-contrast imaging, excellent homogeneity, high resolution, and high transmission.

• FLUAR – the photon collectors: High numerical apertures, good contrast, and out-

standing transmission provide you with enormous optical performance. Ideal for rendering the weakest fl uorescence visible

• Plan-APOCHROMAT – the high-performance series: Best colour correction and highest numerical aper-

tures for brilliant images in all techniques. Convincing: the outstanding point spread function and spherical correction

Unusual in this performance class: the interfaces for DIC. Depending on the upper part, an objective turret with 0, 3 or 6 DIC slide holders is

available with Axio Scope. Six objective positions offer you plenty of scope for mounting them to suit your application.

N-ACHROPLAN objective series: The new inexpensive all-round talent: the N-ACHROPLAN objective series was developed specially for diverse routine applications.

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Imaging system Flexible for the Leading Software and Every Camera Type. The

Simple Step from Stand-alone Stand to Documentation System.

Your Axio Scope Imaging System is more than the sum of microscope, software

and camera: a complete solution from a single source. Integrated fully into the

Carl Zeiss system approach. With security of one hundred percent compatibility –

also for future developments.

Great in all performance classes: the camerasFrom AxioCam ICc1 or AxioCam ICc3, the smallest and most inexpensive digital colour cameras, to the high-speed AxioCam HS: Axio Scope provides a connection for every type of camera. And Carl Zeiss provides the complete range of modern microscope cameras. Each of them carries the Carl Zeiss seal of quality. Axio Scope is also compatible with digital consumer cameras, e.g. from Canon.

Very convenient: AxioVision microscope softwareEasy to operate and functional – with AxioVision you ex-tend your Axio Scope to include digital documentation. The modular microscope software from Carl Zeiss offers you all the important functions in the basic version, from recording and processing to analyzing and archiving your images. AxioVision is one of the leading systems on the market, not least because this software is surprisingly simple to operate despite its wealth of functions. For in-stance, it is possible to adapt the operating interfaces and functions yourself – ideal for simplifying your routine applications through clear processes, thus making them more effi cient. Moreover, you get the AxioVision LE soft-ware version free of charge. A good solution that opens up many options in digital photography.

The free AxioVision LE software provides a variety of high-quality functions for capturing, processing and analyzing images.

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Specimen spaceSpace for Every Specimen. Space for Every Experiment. The Flexibility

of the Specimen Space: a Special Feature That You Will Welcome.

The specimen space can be extended practically as much as you want. A strong

plus point, because there are practically no limits with regard to size of specimen.

Depending on the confi guration the specimen space can be varied continuously:

for specimen thicknesses from 0 to 110 mm.

Where there are no limits to heightThe great scalability of the specimen space is an impor-tant basis for the range of uses of Axio Scope. In addi-tion to the z travel range, it can be infl uenced in several places. 1. You can simply lower the stage carrier at the dovetail. 2. The condenser carrier can also be removed easily, which is practical when you want to lower the stage over the travel range. 3. You can gain space at the in-terface between the upper and lower parts by using a spacer. This comes in 30 or 60 mm versions. Depending on the confi guration, the specimen space can be extend-ed smoothly by up to 110 mm.

The modular interface concept allows easy handling, e.g. when using the spacer (30 mm or 60 mm) to enlarge the specimen space.

Flexible option for further extension of the specimen space in z direction: spacer in 30 mm and 60 mm versions.

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When even more space is neededIf you require even more space for unusually large speci-mens, e.g. in intravital microscopy, Axio Scope offers two vario stands for your refl ected-light and fl uorescence applications. Depending on your requirements, you can couple any upper parts to them. A perfect fi t for your applications.

The specimen space is continuously adjustable for specimen thicknesses from 0 to 110 mm, simply by adjusting the height of the stage carrier.

There is virtually no limit to the size of the specimen space: specimens up to 380 mm in size, such as this tree trunk, can be examined with the vario stand columns.

18

Operating Comfort Convenient to Handle. Effi cient in the Workfl ow. Operating Comfort

and Ergonomics Fulfi ll Almost all Your Needs.

Developed for 24-hour use, Axio Scope will convince you by its quality and stability.

The many intelligent details making your routine work in the laboratory a great deal

simpler and more pleasant to work with.

Well-conceived operating elementsSimply intelligent – the design of Axio Scope makes operat-ing the stand in routine work as comfortable and safe as possible for you. The intensity setting for the transmitted-light illumination is arranged ergonomically around the focus drive. Positioned on both sides close to the focus drive, it is extremely comfortable to operate. You can now adjust the illumination settings without taking your hand off the drive, with your right or left hand, just as you prefer.

New stagesAxio Scope offers a wide range of different stage versions. Two stage innovations are outstanding. 1. The ergo-stage: it enables you to move the stage drive 90 mm along the y axis into the position that is most comfort-able for you. It can be fi xed in that position and easily operated, without any need for you to change the posi-tion of your hand. 2. The new stage with a light special coating: this innovation is ideal for routine applications with a high throughput of specimens. In other words, when many slides are moved on the stage – which used to be the cause of early wear and tear on the stage sur-face. The extremely hard coating of the new stage shows no scratches, even in a hardness test.

Well-conceived operating comfort can be found in many elements in Axio Scope: the stage drive of the new ergo-stage, which can be moved in the y direction and fi xed in place; the focus drive; the 6x fi lter wheel with integrated shutter; and the right/left hand intensity adjustment of the transmitted-light illumination.

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Smart slide guideAn intelligent innovation that experienced clinicians in particular will welcome: the slide guide of Axio Scope alternatively allows you to guide the specimen by hand. Designed as a fl at plate, it is ideal for pushing your speci-men slides back and forth so that you can quickly obtain an overview. If you want to view certain areas at higher magnifi cation, simple let go and use the sensitive stage drive.

Ergonomic tubesDesigned for long laboratory work days, Axio Scope places a special emphasis on ergonomics. The viewing angles of the ergotubes are based on modern studies and are designed for fatigue-free and healthy posture. Two different models are available. The height-adjustable ergotubes with a travel range of 44 mm or the 50-15-50 ergo-phototube with upright correct-side image, which can be adjusted through 50 mm in height and depth at a fi xed viewing angle of 15° so as to be as comfortable as possible for your height. The eyepiece setting can also be varied with both tubes, enabling you to gain an additional 50 mm of height.

Conversion without toolsMore comfortable, faster, and simply practical – the modular interface concept of Axio Scope is designed so that you can simply remove many components, such as the stage carrier or condenser, without any tools. This simplifi es the cleaning process, for example.

Robust constructionWhether pathology, cytology or histology: in clinical rou-tine work, where specimens are often examined in a 24-hour throughput, the material and design of the microscope are important. And here Axio Scope is a true Carl Zeiss microscope: robust stand design and superior mechanical quality.

The extremely durable special coating for toughest continuous use. With the smart slide guide the specimen can be moved around effi -ciently by hand. Alternatively, the more precise x/y stage control can be used at high magnifi vation.

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System Overview

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The New FunctionalityAll the Advantages

N-ACHROPLAN objectives – perfect for routine workThe inexpensive, completely new developed objective series has been calculated for a fi eld of view of 23 mm and is versatile for various applications.

Flexibility of the specimen spaceAxio Scope offers unusually large scalability of the speci-men space – for the most varied applications. Depending on the confi guration, continuously adjustable for speci-men thicknesses from 0 to 110 mm.

Convenient intensity adjustmentThe intensity adjustment of the transmitted-light illumi-nation is arranged on both sides close to the focus drive and extremely comfortable to operate with the right or left hand.

New development: the ergo-stageNewly developed in order to make hand positions indivi-dually adaptable and operation even more comfortable. The stage drive of the new ergo-stage can be pushed 90 mm along the y axis and fi xed in the position that is most comfortable.

Stage for high specimen throughputWith an extremely hard special coating, this new stage remains completely free from scratches even in a hard-ness test – ideal when a lot of specimen slides have to be moved on the stage.

Smart specimen guideAxio Scope specimen guide allows the slide to be moved alternatively by hand – an innovation that experienced pathologists in particular will welcome.

Conversion without toolsMany of the components such as the stage carrier can simply be unscrewed without tools, e.g. for cleaning pur-poses.

23 stand versions – a perfect solutionYou confi gure your Axio Scope from fi ve upper parts, three lower parts and two vario columns. Made to mea-sure for simple routine applications, for demanding research tasks, for every budget.

Countless interfacesAn unusual number of interfaces allows exact adaptation to requirements and budget.

Economical upgradingMany components can be added on by the customer as needed – easily and without additional service costs.

PlasDIC and DIC now in an upright microscopeIn response to many customers a choice of two sophis-ticated contrasting techniques is available in an upright microscope for the fi rst time: Differential Interference Con-trast, DIC, and PlasDIC, the inexpensive relief contrast.

LED in transmitted-light – LED in fl uorescenceAxio Scope offers integrated solutions with these ex-tremely long-lasting and economical light sources, not only in transmitted-light but also in refl ected-light – thus taking into account the growing importance of fl uores-cence as a routine method.

HBO 50 innovationNewly developed for Axio Scope, the burner can now be removed with the drawer – for a particularly easy and comfortable change of burner.

A variety of refl ector insertsThrough its customer interface in the infi nity space, Axio Scope allows users to select the refl ector insert most suitable for the respective application. There is a choice of a 2x slider, a 4x refl ector turret and a 6x refl ector turret.

ISCP – innovative option for transmitted-light applicationsThe interface in the infi nity space can be used not only in refl ected-light (see above, use of refl ectors) but also in transmitted-light: as a fully functioning camera port. Independent of the tube, it can be added on any time.

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Carl Zeiss MicroImaging GmbH07740 Jena, Germany

BioSciences | Göttingen LocationPhone: +49 551 5060 660Telefax: +49 551 5060 464E-Mail: micro@zeiss.de

www.zeiss.de/axioscope