adventures in hrp
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Adventures in HRP. Ellyn Daugherty San Mateo Biotech Career Pathwaywww.SMBiotech.com [email protected] EMC/Paradigm Publishingwww.emcp.com/biotech VWR/Sargent-Welchwww.sargentwelch.com. San Mateo Biotechnology Career Pathway www.SMBiotech.com. - PowerPoint PPT PresentationTRANSCRIPT
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Adventures in HRP
Ellyn DaughertySan Mateo Biotech Career Pathway [email protected] www.BiotechEd.comEMC/Paradigm Publishing www.emcp.com/biotechVWR/Sargent-Welch www.sargentwelch.com
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San Mateo Biotechnology Career Pathway www.SMBiotech.com
Giving motivated teenagers and adults the skills to pursue several career and academic options.
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Partnerships with Industry and Academia
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Biotechnology is an opportunity to teach science in a way that results in science literacy & research skills & career awareness for virtually every student.• Students learn the skills and gain the
confidence and self-directedness that are necessary for independent research(science fair projects) and higher-level science courses.
• Students see meaning and application in their math, biology and chemistry.
• Students learn the science and business of biotech so that they are better prepared to make decisions about their futures and society.
QuickTime™ and aTIFF (LZW) decompressor
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• Text - Chapter 6 and 14• Lab Manual - Labs 6f-6h, 14a• Student CD Tutorials for basic skills• Course Planner/Instructor’s Guide
The HRP Activities are found in Chapter 6
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Biotech 3 HRP labs:• Peroxidase Assay
• HRP extraction from HR Root
• HRP assay using PAGE
• HRP assay w/ TMB indicator
• HRP used in ELISAs
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Hydrogen Peroxidase Activity, in Different Samples, Measured by Bubbling
Sample Amount of Bubbling (5-->0)
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How Do You Make the Phosphate Buffer?
You need 100 mL of a 25 mM NaH2PO4 * H20, pH 7 buffer.You need 100 mL of a 25 mM NaH2PO4 * H20, pH 7 buffer.
0.345 g of NaH2PO4 * H20 is measured measured on a balance and put into an appropriate vessel. Then, solvent is added up to the 100 mL graduation.
0.345 g NaH2PO4 * H20
100 mL
add solvent (dH20) to 75 mLAdjust pH to 7 and add dH20 to 100 mL
conc. desired X volume desired X molecular weight = amount of solute in mol/L in L in g/mol in g
0.025 mol/L X 0.1 L X 138 g/mol = 0.345 g NaH2PO4 * H20
Convert 25 mM = 0.025 mM and convert 100 mL = 0.1 L
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50 kD
35 kD
HRP is 43 kD
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My Professional Goals
• Biotechnology programs in every high school, CTE center, and community college, tailored to the student population and industry and community needs
• Aligned and articulated programs in HS and CC so that teenagers and adults have the same platform from which to enter specialized programs
• To make it as easy as possible for educators to do the other 2
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www.BiotechEd.com
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www.BiotechEd.com
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Get help starting or improving a biotech program !
Ellyn DaughertySM Biotech Career Pathway
www.SMBiotech.com
www.BiotechEd.com
www.emcp.com/biotech
www.sargentwelch.com/biotech
JEFF GANDY 800.848.9033 [email protected]
Holly Ahern512-789-3155holly_ahern @vwreducation.comSandy Duffey
for preview copy [email protected]