Agilent AdvanceBio Desalting-RP cartridges

• Perform MS detection with traditionally non-MS methodologies—such as IEX, SEC, and affinity chromatography

• Save time by desalting your samples online

• Reduce downtime and maintenance by eliminating salt in your MS

• Improve data quality and confidence in your results

ADVANCE YOUR CONFIDENCEFOR BIOTHERAPEUTICS

Agilent AdvanceBio Desalting-RP cartridges

LEARN MORE: www.agilent.com/chem/advancebio

Perform reversed-phase removal of salt ions—online—before MS detection

Characterizing complex proteins—including monoclonal antibodies—requires multiple techniques, such as affinity, ion exchange, and size exclusion chromatography. However, the mobile phases used for these techniques are aqueous-based and contain nonvolatile salts, which can cause signal suppression during MS detection.

AdvanceBio Desalting-RP cartridges enable fast, efficient online removal of salt ions before MS detection. You can use these cartridge-style columns on their own with any LC system to desalt collected fractions. They can also be used on an Agilent 1290 Infinity II 2D-LC system to perform a second-dimension desalt after an affinity, ion exchange, or size exclusion first-dimension separation.

AdvanceBio Desalting-RP cartridge features

AdvanceBio Desalting-RP cartridges are ideally suited for reversed-phase desalting before MS detection. They feature:

• A 10 µm polymeric reversed-phase particle

• Large pore size (1000 Å) for large biomolecules

• Stability from pH 2–13

• Compatibility with all commonly used reversed-phase mobile phases

• Convenient cartridge style (use the Agilent standard reusable holder, P/N 820999-901)

AdvanceBio Desalting-RP cartridges improve the quality of MS data

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Counts vs. Mass-to-Charge (m/z)

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High quality MS

signal suppression

Method Conditions

Mobile phase A: 0.1% formic acid in water

Mobile phase B: 0.1% formic acid in acetonitrile

Flow rate: 0.4 mL/min

Gradient: Time (min) %B 0 5 0.5 5 3.0 80 4.0 80 4.1 5

AGILENT ADVANCEBIO DESALTING-RP CARTRIDGES

Consider Agilent AdvanceBio SEC, Agilent Bio IEX, or Agilent Bio-Monolith Protein A columns for your first dimension.

TIP

REVERSED-PHASE DESALTING FOR ONLINE REMOVAL OF SALT IONS, BEFORE MS DETECTION

Proof of reproducible cartridge performance

2D LC/MS of mAb1 profiles. Injection-to-injection repeatability confirms consistent AdvanceBio Desalting-RP cartridge performance. 1D column: IEX; 2D column: AdvanceBioDesalting-RP cartridges. Second dimension EIC (left) and m/z (right) profiles.

Typical 2D LC/MS system configuration

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Counts vs. Acquisition Time (min)2 3 4 5 6 7 8 9 101112131415161718192021222324252627282930

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Counts vs. Mass -to-Charge (m/z)2400 2600 2800 3000 3200 3400 3600 3800

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2nd Dimension: MS (EIC, 48+) 2nd Dimension: m/z spectrum

Pump 1 Injector 1D column

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QTOF MS

Salt to Waste IEX/SEC/ProteinAcolumn

AdvanceBioDesalting-RP cartridge

Part number Description

PL1612-1102 AdvanceBio Desalting-RP, 3/pk2.1 x 12.5 mm

820999-901 Cartridge holder

For Research Use Only. Not for use in diagnostic procedures.

© Agilent Technologies, Inc. 2016Published in USA, August 1, 2016

5991-7067EN

To learn more, or to order now, contact your Agilent sales representative at

www.agilent.com/chem/contactus

Advance your confidence for biotherapeutics with our full AdvanceBio portfolio

• AdvanceBio SEC mAb: Aggregation analysis with increased speed, resolution, reproducibility and sensitivity

• AdvanceBio Glycan Mapping: For preparation and analysis of 2-AB labeled N-linked glycans

• AdvanceBio RP-mAb: The only reversed-phase column designed specifically for mAbs

• AdvanceBio Oligonucleotide: For accurate and reliable characterization of impurities

• AdvanceBio Peptide Mapping: Quickly resolve and identify amino acid modifications in primary structure

• Agilent Bio-Monolith Protein A and G: For titer determination and purification via affinity chromatography

• Agilent Bio mAb and IEX: For charge variant analysis via ion exchange chromatography

www.agilent.com/chem/advancebio