acquired immunodeficiency syndrome presented by : deepti awasthi
TRANSCRIPT
CONTENTS• Introduction• History• Epidemiology• HIV Virus• Routes of
transmission• Pathogenesis
• Clinical signs and manifestations
• Oral manifestations
• Lab diagnosis• Prophylaxis• Treatment• Universal
precautions• References
INTRODUCTION
• Since, the initial recognition of AIDS in the US in 1981,tremendous advances have taken place in the understanding of this dreaded disease in the last decade as regards its epidemiology, etiology, immunology, pathogenesis, clinical features & morphologic changes in various tissue and organs of the body.
• .• 1st DEC - world AIDS day by the WHO.
EPIDEMIOLOGY
• Acc. To WHO, in nov 2003
40 million - infected worldwide
5 million – newly infected
3 million – died
2.2 million children - <15 yr • In india,
2nd largest after south africa
In 2003, 5 million infected
Status of HIV epidemic in India
High Prevalent states
Maharashtra
Manipur
Andhra
Pradesh
Nagaland Tamil
Nadu Karnataka
HISTORY
• In monkeys – for over 100,000 years• In 1956 – in central africa : “ gay fever ”• In 1981 – first indication came from new
york & los angeles• In 1983 – Luc Montagnier & colleagues
from pasteur institute ,paris , isolated a retrovirus – LAV
• In 1984 – Robert gallo & colleagues , USA : HTLV- III
• In 1985, serological tests available for anti-HIV antibodies.
• In 1986, international committee decided on the generic name - HIV
• In india –
1st case reported in chennai
HIV virus
• Lentivirus subgroup, family retroviridae.• 2 forms :
HIV-1 : US & central africa
HIV-2 : west africa & india
ROUTES OF TRANSMISSION
• 1. SEXUAL CONTACT
- 75% of all cases
- male to male & male to female is more potent route than female to male.
• 2. PARENTERAL
- 25%
1) I.V. Drug abusers
2) hemophiliacs
3) blood recipients
• 3. PERINATAL• Vertical transmission • Several risk factors:
- pre term delivery
- low maternal antenatal CD4 count
- illicit drugs during pregnancy.
- elective cesarean delivery – by 87% +
ZVT in the mother & infant.
• Besides blood ,HIV has been isolated from no. of body fluids & tissues :
semen, vaginal secretions , cervical secretions, breast milk, CSF, synovial, pleural, peritoneal, pericardial & amniotic fluid.
• Salivary protein – secretory leucocyte PI
anti –HIV activity
• STERILIZATION & DISINFECTION
• HEAT : Virus is very fragile & can be eliminated easily by hot water at 56% for 30 mins.
• CHEMICALS : NaOCl – 0.1%
Ethanol – 70%
Formaldehyde – 5%
Glutaraldehyde – 2%
H2O2 – 0.3%
PATHOGENESIS Interaction of gp120 of HIV to CD4+T cell
internalisation of virion
uncoating of virion
reverse transcriptase
proviral DNA
unintegrated, integrated
Activated CD4+T cell inactivated CD4+T cell
budding,syncytia LATENT PHASE
CYTOPATHIC PHASE
Quantitative depletion qualitative failure to respond
• HIV infection of nervous system :
• Out of non-lymphoid organ involvement, HIV inf of nervous system is the most serious.
• Some presenting features include :
acute aseptic meningitis
subacute encephalitis
vacuolar myelopathy
peripheral neuropathy
STAGES
1) ACUTE HIV INFECTION
• 3-6 wks – 50% persons experience low grade fever, malaise, headache, lymphadenopathy. Resolves within wks
• Tests for HIV antibodies are –ve at onset & becomes +ve during its course- “SEROCONVERSION ILLNESS” .
• P 24 antigen
2) ASYMPTOMATIC OR LATENT INFECTION
• All persons pass through this phase which may last upto several years.
• +ve HIV antibody tests • This period of clinical latency , does not
mean microbiological latency as virus replication goes on throughout.
• The CD4+T cell count decreases
< 200 = clinical AIDS sets in
3) PERSISTENT GENERALIZED
LYMPHADENOPATHY
4) AIDS RELATED COMPLEX (ARC)
• Includes various constitutional symptom : unexplained fever > 1mth weight loss > 10% chronic diarrhoea > 1mth• Oppurtunistic infections
• 5) AIDS
• End stage disease• Irreversible breakdown of immune
defence mechanism• Progressive oppurtunistic infection &
malignancies.
OPPURTUNISTIC INFECTIONS & MALIGNANCIES
• MALIGNANCIES:• Kaposi sarcoma• Lymphoma• BCC• Melanoma
• BACTERIAL
• TB• Salmonellosis• Campylobacter inf• Nocardia & actinomycetes• Legionellosis
• PARASITIC
• PNEUMOCYSTITIS
CARINII PNEUMONIA
• TOXOPLASMOSIS
• MYCOTIC
• Candidiasis• Cryptococcosis• Aspergillosis• Histoplasmosis
ADVANCED HIV/AIDS DISEASE DEFINITIONS FOR
SURVEILLANCE FOR ADULTS
Any clinical stage 3 or stage 4 disease
or,
where CD4 is available, any clinical stage and CD4 <350/mm3
• Diff. b/w adult & pediatric AIDS:
• Children develop humoral immunodef. early, leading to recurrent bacterial inf.
• Failure to thrive, chronic diarrhoea ,lymphadenopathy, TB – common manifestation.
• Lymphocytic interstitial pneumonia- seen mostly in children.
LAB DIAGNOSIS OF AIDS
• A. IMMUNOLOGICALTESTS
• Leucopenia• Lymphopenia• Thrombocytopenia• CD4+T cell < 200 / mm3• T4 : T8 is reversed.• Lymph node biopsy
• B. SPECIFIC TESTS :
1. Antigen detection• The major core antigen , P24, earliest
virus marker to appear in blood.• IgM antibodies appear in 4-6 wks,
followed by IgG antibodies.
2. Virus isolation & culture• From peripheral lymphocytes• Viral replication can be detected by-
reverse transcriptase activity
3. POLYMERASE CHAIN REACTION• Most sensitive & specific test• Gold standard for diagnosis in all stages• 2 forms : DNA & RNA
4. ANTIBODY DETECTION• Simplest & most widely employed technique.• 2-8 wks to months for antibodies to appear• Highly infectious• Seronegative infective – “window period”• 2 types : screening & confirmatory tests
• Screening tests – high sensitivity
not highly specific
false + ve results
The most widely used screening test is ELISA.
• Confirmatory tests – WESTERN BLOT.
In this HIV proteins are seperated acc. to their electrophoretic mobility by poly- acramide gel electrophoresis are blotted onto strips of nitrocellulose paper.
PROPHYLAXIS• The prevention aims at –
Health education
Identification of sources
Elimination of high risk activities• No specific vaccine is available.• Several possible strategies have been
explored for vaccine production. These include immunisation with –
a) Modified whole virus
b) Subunits
c)Target cell protection by anti-CD4 antibody
TREATMENT1. Treatment & prophylaxis of infections &
tumours
2. General management
3. Immunorestorative measures-
Administration of IL-2, thymic factors ,leucocyte transfusion & bone marrow transplantation.
4. Specific anti – HIV agents
NRTI • Zidovudine , stavudine , lamivudine
Didanosine , abacavir. NNRTI• Nevirapine, delavirdine PI• Saquinavir , ritonavir , indinavir FI• Enfuvirtide Integrase I• Raltegravir CCR5 antagonist• Maraviroc
PREVENTION• Safer sex methods• Screening of blood donors• Disposable syringes, needles etc• Infected women – advised against
pregnancy• For interruption of perinatal transmission
ZVT 200 mg TID to the women & continued during delivery
decreases rate to < 8 %.
IRIS• When a pt. starts ART, his immune
deficiencies improve. This sometimes results in uncontrolled inflammatory responses. Hence, pt. may show worsening of clinical features or lab parameters inspite of improving CD4 counts & decrease viral load.
• TREATMENT
- Symptomatic - NSAIDS
- severe: prednisolone - life threatening: stop ART
PEP
• ART should be started within the first few hours & no later than 72 hrs .
• HIV testing should be done initially & following 3 & 6 months.
• EXPOSURE
Less severe - 2 drug PEP
more severe - 3 drug PEP
• Biohazard to Dental workers :
• Larger quantity of blood loss• Repeated blood to blood contact• Longer• Length of surgical procedure• Needle prick injuries
UNIVERSAL PRECAUTIONS
• Alert all the time.• Single chair room• Gloves – examination• Dental units covered with water proof
sheets.• Impervious surgical gown, cap & mask. • For procedure – double gloves• Airotor use – avoided
• In suction bottle – 2% glutaraldehyde 30 ml
2% NaOCl 60 ml
• Needles discarded immediately
• Bag containing waste – incineration• Instruments – reautoclaved twice by double
sterilization
Spillage of blood & body fluids , area saturated with 1% NaOCl for 30 mins. Then mopped with an old linen towel.
References :• Ananthanarayan and paniker’s textbook of
microbiology ; 8th ed.• Kliegman, behrman, jenson,stanton. Nelson
textbook of pediatrics ; vol.1• Harsh mohan . Essential pathology ; 3rd ed• Mehta PJ. Practical medicine ;19th ed• Chandra S, chandra S. Textbook of
pedodontics. 2002• Davidson S. principles & practice of
medicine;19th ed.• www.google.com
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