Tropical Soil Bacteria Isolation & Characterization

Tornés-Blanco Anibal Alicea-Pauneto Abneil D. Colón-Rivera Ramón B. González-Piñeiro Ann

Results

Fieldwork Data I

Sample

Extraction

Date & Time

Collected

Site

FeaturesHumidity Temperature pH

Sample A -

Centro Médico

01/28/15

5:42 pmUrban Moist 302 K 7.33

Sample B-

Spout

01/29/15

6:45 amSemi Rural Moist 296 K 6.50

Fieldwork Data II

Sample ExtractionDate & Time

CollectedSite Features Humidity Temperature pH

Sample C -

Asomante,

Aibonito

01/29/15

7:30 a.m.Semi Rural Moist 293 K 5.00

Sample D- Toita,

Cayey

01/28/15

5:30 pmRural Moist 298 K 5.50

Soil Recollection w/Coordinates I

Sample A - 18°23'45.3"N 66°04'27.0"W Sample B - 18°12'26.9"N 66°03'10.4"W

Soil Recollection w/Coordinates II

Sample C - 18°8'3"N 66°16'23"W Sample D - 18°14'42.2"N 66°20'20.6"W

Characterization Results I

Bacteria Designator Code Samples PCR & Electrophoresis Bioinformatic Analysis

ATB 30R01A Sample A + N/A

ADAP 30R01A

Sample B

+ N/A

ADAP 30R01B + N/A

Characterization Results II

Bacteria Designator Code Samples PCR & Electrophoresis Bioinformatic Analysis

RBCR 30P01A Sample C - N/A

AMGP 30P01A Sample D - N/A

R2A Purifications ISample A

Sample B

ADAP30R01A

ADAP30R01B

ATB30R01A

Purifications IIISP4 (Sample C) LB Medium (Sample D)*

f*Experimental Variation*

Gram Stain I - Gram PositiveSample A

Sample B

ADAP30R01A

ATB30R01A ADAP30R01B

Gram Stain II - Gram PositiveSample C Sample D

RBCR 30P01A AMGP 30P01A

Morphology I

Sample A:

- Form: Circular - Elevation: Raised - Margin: Entire

Sample B:

- Form: Circular - Elevation: Convex - Margin: Entire

Morphology IISample C: - Form: Circular - Elevation: Flat - Margin: Curled

Sample D: - Form: Circular - Elevation: Convex - Margin: Entire

Antibiotic Production ISample A Sample B

Antibiotic Production II Sample D

Sample C

M. Lutheus (-) E. Coli (-)M. Lutheus (-) E. Coli (-)

Electrophoresis #1 - Samples A-DSlots 1-12

B

C

A

D

Slots 13-24

Acknowledgments ● Howard Hughes and RISE Program at

UPR Cayey ● Dr. Michael Rubin ● Mr. Giovanni Cruz ● National Institute of Health