A NOVEL LOW-COST

MINIATURE PROBE FOR ORAL DIAGNOSIS

Thair Takesh, Afarin Anbarani, Jessica Ho, Vania Firmalino,

Petra Wilder-Smith

Beckman Laser Institute University of California, Irvine

Ron Liang: College of Optical Sciences, University of Arizona, Tucson

Background• More than 526,481 incident cases annually.

• 6th most lethal form of cancer.

• 150,000 new cases and >10,000 deaths annually in US.

• Advanced lesions outnumbered localized lesions more than 2:1 of all

oral cancers

• Five-year survival rate is 75%, 16% for those with metastasis.

• Clinical problems: Specialist, Invasive with functional and esthetic

implications, sampling, Field cancerization, Poor patient compliance

• Gold standard treatment is surgical biopsy.

• New techniques are required.

Goals

(1) Design and construct a low cost mini-probe for smart phones that combines high resolutionpolarized white light images (pWLI) and auto fluorescence imaging (AFI).

(2) Test probe design, ergonomics and functionality .

Materials and MethodsImaging Probe

Optical concept of the proposed mobile phone imaging device for oral cancer screening. All components in black are built-in components in mobile phone, others inside the red box are components in the intra oral imaging probe.

Materials and Methods• 15 human subjects in full compliance with UCI IRB-

approved protocol 2002-2805.

• 10 subjects with healthy oral mucosa and 5 subjects with leukoplakia.

• 6 standard oral sites imaged in each subject.

• 3 dual-modality images recorded for each site.

Materials and Methods • Clinicians and subjects evaluated the device’s

– ease and comfort of use

– size and shape-compatibility with the mouth

– weight

– imaging speed

– clarity and color of images

– resolution and magnification

– detection of differences between healthy and diseased oral mucosa

ResultsHigh-resolution pWLI mapped mucosal color, texture and vascularity at levels of high resolution.

ResultsAFI images of healthy oral tissues showed a characteristic strong green AFI signal.

ResultsAFI images of pathological oral mucosa showed areas of dark reddish-brown signal.

Results

• AFI signal differed minimally between subjects for any 1 specific imaging location.

• AFI signal differed in intensity and variability between each of the 6 imaging locations in the same subject and

between subjects.

Conclusion• Effective design and appropriate for clinical use.

• recorded data in subjects with healthy and pathological oral mucosa.

• Observed site specific AFI characteristics.

• Healthy tissues were differentiated from pathological ones.

• Next steps:

- Re optimize intra-oral imaging probe.

- Developing image processing software.

- Clinical validation studies.

• We gratefully acknowledge funding from NIH: award # 1UH2EB022623-01

Thank you