a contract research and services organization. ideas to life! a contract research and services...
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Ideas to Life!
A Contract Research and Services Organization
Xcelris is a Specialty Contract Research and Services Organization
Delivering the best end to end solutions to global Genomics, Pharma and Bio-Pharma Community
Built by team of Scientists and Management Professionals
Outstanding infrastructure facilities to support Cutting Edge Research
Ideas to Life!
Our Vision
“To lead the way to a healthier world by partnering with Pharma & Biotech Community across the globe and delivering high quality value added healthcare solutions.”
Ideas to Life!
Mission
“Xcelris endeavors to be a world class Lifesciences based innovative service organization built towards achieving excellence in;
Research & Technology
Service Delivery
Management Capability”
Ideas to Life!
Infrastructure
cGLP and cGMP compliant Laboratory designed in accordance with global standards
Spread across 50,000 square ft. area
Fully equipped Labs conforming to global norms
State–of–the–art equipments
Dedicated LIMS
Dedicated lab for Genomics and Bioinformatics.
Ideas to Life!
People
Committed & enthusiastic high performing team of 50 members
More than 100 man-years of experience in Molecular Biology, Plant Molecular Biology, Genome Sequencing, Animal Genetics, Virology, Bioinformatics, Biotech and analytics
Alliance partner having experienced people from premier institutions like Sanger Labs, Baylor College which were part of HGP.
Xcelris Scientific Board with a strong experience in the field of Genomics & Biopharmaceuticals.
Ideas to Life!
Whole Genome Sequencing
Next Generation Sequencing Technology Platforms using ABI & Roche
Whole Transcriptome Analysis and SNP Discovery
Ideas to Life!
Next Generation DNA Sequencing Technology
SOLiD technology: Two slide per run and up 16 samples /run. 2 Base encoding with higher fidelity. 50-75 base per run. 10-15 GB data per run.
Phase I for ABI SOLiD: Wet Lab
Preparation of high quality DNA.
Qualification of DNA using AGE/Picogreen.
DNA Shearing and End repair.
Fragment Library or Mate Paired Library.
Clonal Emulsion PCR and Bead Enhancement.
Bead Deposition and Ligation Sequencing.
Raw data Generation.
Ideas to Life!
GS FLX technology
Phase I for GS FLX: Wet Lab
Sample Type: Genomic DNA, PCR product, BAC and cDNA.
Qualification of DNA using AGE/Picogreen.
DNA fragmentation 300-800 bp and End repair and adaptor A&B ligation.
Water-in-oil emulsification of beads and PCR reagents.
Clonal amplification in microrector.
DNA Bead Deposition to Picotitre plate and Pyrosequencing.
Raw data Generation.
One slide per run and up 16 samples /run. 300-400 bp per read. 1.2 million reads per run 0.8-1.0 GB data per run.
Ideas to Life!
Roche GS FLX (454) Technology
Sequencing Workflow : Loading of Pico Titer Plate Device
Well diameter: average of 44 µm> 400,000 reads obtained in parallel
A single clonally amplified sstDNA bead is deposited per well
Depositing DNA beads into the Pico Titer Plate device
Quality filtered bases
Amplified sstDNA library beads
Ideas to Life!
Roche GS FLX (454) Technology
A A T C G G C A T G C T A A A A G T C A
C TA
Repeated dNTP flow sequence:
GGTCAGTCAGTTTTCAG GAT CCCGATT
G CT A
Anneal Primer
Process continues until user-defined number of nucleotide flow cycles are completed.
Sequencing-by-synthesis
Ideas to Life!
Xcelris Capability
Phase IV: Bioinformatics
Assembly and Annotation.
Location of genes/promoters
Transcribed Regions
Function of Motifs/other DNA seq
Screening of data against bacteria/virus and vector data bases.
Detection of sequence variations
SNP/CNV/Dupli/In-Del
Phase II: Data processing
Genome Data processing,
Align & Create color space consensus
Convert color space consensus to base space for SOLiD.
Draft Assembly.
Final Assembly and Annotation.Removal/Identification of the gaps.
Phase III: Genome Finishing
Gap closure by PCR-assisted contig extension (PACE) methodology. Contig Gap filling and customized
Ideas to Life!
Deliverables
Alignment Report -A summary of the mapping results, including score distribution.
Filter report (Optional) Score distribution plots MAX file -A MAX file contains all the reads that map to genomic reference.
Does not include mapping to splice junctions. General Feature Format (GFF) file -A GFF file containing all the reads that
were mapped uniquely, including those mapped to splice junctions. Counts file-A file in GFF format, containing information regarding the
number of reads hitting the corresponding region (Figure 2). Annotation wig file-Coverage file in wiggle format for regions contained in
the annotation file, one for each genomic region (chromosome) -- Predicted Transcribed Region (PTR) GFF files
-- Optimization plots-These plots are used in selecting the optimal parameters for detecting Novel Transcribed Regions (NTR).
-- Annotated Transcribed Regions (ATR) plots