biobench contract research organization - stlcc.edu :: users
TRANSCRIPT
Located within new St. Louis Community College Campus
Center for Plant and Life Sciences
BioBench Contract Research Organization
Do you have time-consuming laboratory tasks that occupy your
personnel’s time?
BioBench Contract Research Organization
Do you lack access to necessary instrumentation for certain types of experiments?
BioBench Contract Research Organization
BioBench Contract Research Organization
We are the new
BioBench Contract Research Organization (CRO)
Located within the new St. Louis Community College campus
At the Center for Plant and Life Sciences
BioBench Contract Research Organization
Located within new St. Louis Community College CampusCenter for Plant and Life Sciences
• Cloning – Fragment isolation– Ligation
• Isolation (large or small scale)– Genomic DNA– Plasmid DNA– RNA
• Agilent 2100– DNA, RNA and protein analysis
Nucleic Acid
• Sequencing– DNA isolation
– Sample preparation
– Sequence analysis
– Primer design
• Short Fragment Analysis– Genotyping
– Data interpretation
Capillary Electrophoresis
• Experienced with a large variety of cells– Adherent cell types– Suspension cell types
• Transient transfections– Lipid-based and peptide-based reagents– Electroporation and nucleofection
• Stable cell line generation
• Cryopreservation of cultures
Cell Culture
• Fluorescence Assisted Cell Sorting (FACS)
– For analysis and culture enrichment
• Labeling of cells in culture
• Fixing and Fluorescent Staining
• Staining of live cell cultures (direct immunofluorescence)
• Recording staining results by photography
Advanced Cell Culture Work
• Experimental design
• Screening of large number of siRNAs
• Detection of knockdown
• Data interpretation
RNA Interference
• SYBR Green and probe-based expertise
• Singleplex assay design (primer design)
• Multiplex assay design (primer/probe design)
• Assay optimization
• Extensive troubleshooting skills available– Can help with problematic existing assay (s)
• Data Analysis– Absolute quantitation– Comparative quantitation
Quantitative PCR
• Genotype Assay Development
• Screening of animal colonies
– Genomic DNA isolation from tail snips
– Transgenic or knockout animal identification
• Founder animal screening
Colony Work
• Various assays
– Luciferase
– Other activity-based assays
• Quantitation
• Immunoprecipitation (IP)
• SDS-PAGE
• Western Blotting
• ELISA
Protein
• If you have an idea, and you do not see it addressed here, please contact us….
• We are happy to work with you to
develop a project that will fit your needs
Don’t see what you need?
• Senior Research Scientist, CRO Coordinator
– Elizabeth (Betsy) Boedeker
• 314 513 4966
• Director, Plant and Life Sciences Center
– Richard Norris, PhD
• 314 513 4951
Contact Information