6.huong dan pha che va kscl moi truong vs

5/24/2018 6.Huong Dan Pha Che Va KSCL Moi Truong Vs

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HNG DN PHA CH V KIMSOT CHT LNG MI TRNG

NUI CY VI SINH VT

ISO/ST 11133 -1&2: Microbiology of food and animal

feeding stuffsGuidelines on preparation and productionof culture madia


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NI DUNG

Hng dn pha ch v m bo cht lng cami trng nui cy vi sinh vt

Hng dn th nghim mi trng nui cy visinhvt

Part 1: General guidelines on quanlity assurance for preparartion of culture madia in the

laboratory

Part 2: Practical guidelines on performance testing of culture madia


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Hng dn pha ch v m bo chtlng mi trng nui cy vi sinh vtdng cho phng th nghim


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Cc thut ng

L mi trng: l n v ng nht, c th truy

nguyn y s lng t nguyn liu, bn thnhphm, thnh phm m sn phm ny c snxut trong iu kin c kim sot v t chtlng


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Thut ng

Mi trng sn sng s dng (ready to use)

Mi trng hon chnh sn sng s dng Mi trng hon chnh phi un tan, a hay phn

phi vo n v s dng

un tan, b sung cc mt vi thnh phn khc


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Thut ng

Mi trng bt kh tng hp dng

tng hp han chnh: vd. PCA, XLD Cha hon chnh:vd: Baird Parker agar, Fraser broth

Mi trng tng hp t cc thnh phn theo cng

thc


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Kim sot cht lng mi trng

H s lu tr ti PTN H s t nh sn xut

Tn v m s, cng thc thnh phn v cht b sung ca nhsn xut

S l (batch No) pH khi pha ch hon chnh iu kin lu tr, hn dng Chng nhn cht lng, chng VSV kim sot Kt qu kim tra ca nh sn xut Cnh bo an ton (nu c)


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H s lu tr

Cc thng tin cn kim sot khi nhn sn phm

M s sn phm Tnh nguyn vn ca bao gi

Ngy ht hn ca sn phm

Cc h s km theo


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Lu tr

Khi s dng mi trng thng mi phi tun th

theo hng dn ca nh sn xut v iu kin lu tr Ngy ht hn s dng

Hng dn s dng


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Mi trng tng hp dng bt kh

Tun th nguyn tc vo trc ra trc (first in - firstout)

Kim tra trc khi m hp Nim phong

Ghi ngy m u tin ln chai

nh gi tnh trng mi trng bn trong

S bin i cht lng sau khi m hp Thay i do iu kin lu tr

Biu hin thay i: kt cu vt l, mu sc


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Bo qun mi trng sau pha ch

Bo qun trnh s bin i cht lng

Trnh nh sng Bo qun trong t mt 2-8oC

Mi trng c cht khng bn: s dung trongngy

nh gi thi hn bo qun thng qua vic kimsot cht lng


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Pha ch mi trng ti PTN

Pha ch mi trng ng l bc u quan trng trongvic cho kt qu phn tch chnh xc

Tun th theo hng dn m bo cht lng ca PTN vca nh sn xut

Lu h s tt c cc d liu lin quan:khi lng/th tch,pH, ngy, iu kin thanh trng

Mi trng pha ch t cc thnh phn ring l: thc hinchnh xc theo cng thc, lu HS tt c cc d liu linquan, nh s xc nhn


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Pha ch ti PTN

Nc dn in: 25S/cm (0,4Mcm)

Mt VSV: khng qu 103CFU/ml, tt hn


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Pha ch ti PTN

Ha tan

Ha tan cn nhanh cn tt

Mi trng agar: ngm trc khi un tan o v chnh pH

Chnh pH trc khi thanh trng

o kim tra sau khi thanh trng v han chnh mi trng

o pH sau khi lm ngui n 25oC

Chnh pH bng NaOH 1N (40g/lt); hoc HCl 1N (36,5g/l)

Phn phi Khng cha qu 80% th bnh cha


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Kh trng mi trng

Hp kh trng

Hp 121oC/15 pht Nu th tch cha cng ln, kh nng qu nhit cng

cao

H nhit ngay khi thi gian kh trng

Th tch bnh cha > 1lt Cha cc thnh phn nhy nhit


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Cc thnh phn nhy nhit

Khng c hp cc mi trng c thnh phn

nhy nhit Brilliant green Mui mt

Mui st

Vd: XLD agar, TCBS agar, VRB/VRBD agar


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Kh trng mi trng

Lc kh trng Dng kh trng cc cht/mi trng nhy nhit

Thit b lc c l 0,2m


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Kim tra sau khi hon chnh

Mi trng sau khi pha ch han chnh phi kim

tra pH

Mu

v trng

Biu hin vt l bn ngoi


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Chun b cht b sung

Cht b sung thng l cc cht c c tnh cao

Cn hat tnh khi trong hn s dng Khng ti ng lnh nhiu ln


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un chy mi trng

un chy mi trng agar bng cch un cchthy

Ngng un ngay khi mi trng tan chy Lm ngui ngay trong b iu nhit 45-50oC Nhng mi trng trc tip vo mu phi

c gi 44-47o

C Khng nn gi mi trng qu 4h sau khi tan

chy


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Kh khng kh trong mi trng

un cch thy trong nc si khong 15 pht, ni

lng np ng Vn cht np v lm lnh nhanh n nhit s

dng


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Thm cht b sung

Ch thm cht b sung khi mi trng ngui n

45-50

o

C Cht b sung phi duy tr nhit phng trc

khi cho vo mi trng

Thao tc nhanh trnh MT agar vn cc


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Chun b v bo qun mi trng trn a

Petri

dy mi trng: t nht 3mm

Phi dy hn nu a c nui 48h hay 40oC

Dn nhn v ghi tn MT, ngy pha ch/ht hn Trong qu trnh c th lm thay i m mi trngthnh

phn mi trng thay i, nh hng n s pht trin ca VSV.Cc yu t nh hng n m mi trng nh: loi t m,

m khng kh, p sut trong t, s lng a


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Bo qun a mi trng

Bo qun trong t mt 2-8oC

Trnh nh sng Bo qun trong cc ti nylon s lu hn

Khng lm kh mt a trc khi bo qun trongt lnh


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Thi hn bo qun mi trng sau pha ch

Ty thuc loi mi trng,thnh phn cc hatcht trong mi trng

Thi hn s dung ca tng mi trng phi cnh gi bng cc chng VSV


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Thi hn bo qun mi trng sau pha ch

Loimi trng Dngccha iukinboqun ThihnsdngMi trng rn: Chai Tlnh280c 6 thng

ngnghim T lnh 2 80c 2 thng

a T lnh 2 80c 1 tun hay th hai utun sau

Mi trng lng

v dung dchphalong:

Chai 400ml Tlnh280c 2 thng

Chai 1000ml Nhit phng 1 thng

ngnghim T lnh 2 80c 1 tun


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Chng VSV trong kim sot cht lng mi

trng

Mi mi trng phi s dng cc chng VSV sau Chng c biu hin dng tnh

Chng c biu hin dng tnh yu

Chng c biu hin m tnh

Chng b c ch hon ton hay mt phn


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Theo yu cu ISO 11133

Mi trng ready to use: Khng cn th nghim cht lng C chng nhn cht lng ca NSX

Bo qun v s dng ng hng dn

Pha ch t MT kh tng hp Mi trng phn lp hay nh lng: th nghim nh lng

hay bn nh lng

Mi trng th nghim sinh ha: th nghim nh tnh


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Pha ch t MT kh tng hp (tt)

MT khng cha cht ch th hay nhn t chn lc: sdng 1 chng VSV dng tnh

MT cha cht ch th hay nhn t chn lc: s dng tnht 1 chng c biu hin dng tnh v 1 chng c

biu hin m tnh


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Mt s biu hin bt thng ca MTMT agar khng ng -Qu nhit

-pH qu thp

-Khi lng agar khng

-Agar khng tan-Khng c lc k khi un tan

pH thay i -Qu nhit

-Nc khng t cht lng

-Pha tp cc thnh phn khc

-

o pH khng ng nhit -My o pH khng c hiu chun

Mu MT thay i -Qu nhit

-Nc khng t cht lng

-Mi trng kh/thnh phn khng t CL

-Khng ng pH

-Pha tp cc thnh phn khc


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Mt s biu hin bt thng ca MT

Kt ta -Qu nhit-Nc khng t cht lng


-Khng ng pH

MT b c ch/VSVpht trin km

-Qu nhit

-Nc khng t cht lng, cha nhiu cht c


-Pha/tng hp khng ng cng thc

-Bnh cha/ng a khng sch

Tnh chn lc km -Qu nhit-Mi trng kh/thnh phn khng t CL

-Pha/tng hp khng ng cng thc

-Cht b sung cho vo khi qu nng hay khng

ng nng -Cht b sung b nhim


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Mt s biu hin bt thng ca MT

B nhim VSV Kh trng khng ngcch

K thut kh trng khngt yu cu

Cht b sung b nhim

6.huong dan pha che va kscl moi truong vs

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