598 PATENT

The complete coding sequences and amino acid sequences for both canine and human 32K al- veolar surfaetant proteins (ASP) are disclosed; clones for the 10K protein have also been ob- tained. Methods and vectors for obtaining these proteins in recombinant form are described. The availability of large amounts of these proteins through recombinant techniques permits the use of ASP in suitable pharmaceutical compositions in the treatment of respiratory deficiency syn- dromes.

4912039

M U L T I D R U G R E S I S T A N C E I N M A M M A L I A N C E L L L I N E S A N D I S O L A T I O N O F D E T E R M I N A N T

G L Y C O P R O T E I N D N A

John R Riordan, Toronto, Canada assigned to HSC Research Development Corporation

A method for isolating a eDNA specific for P- glycoprotein is disclosed. The P-glycoprotein is the determinant in the multidrug resistance phenotype exhibited by living cells. The nucleic acid equivalent of the eDNA may be cloned in a recombinant plasmid or phage. The eDNA may also be used as a probe in determining multidrug resistance in cells. The DNA molecules specific for the P-glycoprotein is in the form of a gene family where various members oftbe gene family are amplified to varying degrees in multidrug resistant cells from various sources.

4912040

E U C A R Y O T I C E X P R E S S I O N S Y S T E M

Randal Kaufman, Andre Dorner assigned to Genetics Institute Inc

This invention provides vectors, improved host cells and improved methods for producing a heterologous protein by culturing an improved eucaryotic host cell of this invention trans- formed or transfected with a vector capable of directing the expression of the heterologous pro- tein. The preferred improved host cell of this in- vention is a mammalian host cell containing and capable of expressing an anti-sense GRP78 DNA sequence.

ABSTRACTS

4912044

P R E P A R A T I O N O F M E S O P H I L I C M I C R O O R G A N I S M S W H I C H

C O N T A I N A D - H Y D A N T O I N A S E W H I C H IS A C T I V E A T E L E V A T E D

T E M P E R A T U R E

Elard Jacob, Karsten Henco, Stefa Mar- cinowski, Gerhard Schenk, Eisenberg, Federal Republic Of Germany assigned to BASF Ak- tiengescllschaft

A description is given of a genertic engineering process for the preparation ofmesophilic micro- organisms which contain a hydantoinase active at elevated temperature, and of DNA sequences which code for this enzyme.

4912046

P O R T A B L E I N D U C I B L E C O N T R O L S Y S T E M

Dennis J Henner, Daniel G Yansura assigned to Genentech Inc

Shuttle vector systems are provided for the regulated expression of subject genes in trans- formed hosts. A promoter/operator which does not originate with the transformed host cell con- trols expression of the subject gene and a repressor, which also does not originate with the host cell, regulates the promoter/operator. Ac- cording to this invention, promoter/operator- repressor functionalities from one bacterium are used in other bacteria, thereby obviating the need to locate homologous expression regulatory sequences for each bacterial host.

4912206

C D N A C L O N E E N C O D I N G B R A I N A M Y L O I D O F A L Z H E I M E R ' S

D I S E A S E

Dmitry Y Goldgaber, Michael Lerman, D Car- leton Gajdusek assigned to The United States of America as represented by the Department of Health and Human Services

Four clones have been isolated from an adult human brain cDNA library using an

PATENT ABSTRACTS

oligonucleotide probe corresponding to the first 20 amino acids of the brain amyloid polypeptide of the Alzheimer's disease. The open reading frame of the sequenced clone coded for 97 amino acids including the known amino acid sequence of this polypeptide. The 3.5 kb messenger RNA has been detected in mammalian brains and human thymus. The gene is highly conserved in evolution and been mapped to human chromo- some 21.

599

Hybrid, third generation, plasminogen ac- tivators containing plural, heterologous poly- peptide kringles prepared by recombinant DNA techniques as well as the genes coding for the ac- tivators, Vectors containing those genes and a method for using the plasminogen activators as thrombolytic agents, are disclosed.

4912207

DNA C L O N E O F H U M A N T H R O M B O M O D U L I N AND

P O R T I O N S T H E R E O F

Philip W Majerus, J Evan Sadler assigned to Washington University

A cDNA having a base sequence for human thrombomodulin has been cloned and charac- terized and the amino acid sequence of the human thrombomodulin has been determined.

4915726

B I O L O G I C A L C O N T R O L OF D O D D E R

Thomas A Bewick, Jana S Stewart, Larry K Bi- nning, Walter R Stevenson assigned to Wisconsin Alumni Research Foundation

A mycoherbicidal inoculum is disclosed for con- trolling the growth of dodder (Cuscuta spp.) on field crops. The inoculum is obtained from spores of Fusarium tricinctum, a species of AI- ternaria, and their mutations.

4916071

P O L Y - K R I N G L E P L A S M I N O G E N A C T I V A T O R

Paul Hung, Narender K Kalyan, Shaw-guang L Lee assigned to American Home Products Cor- poration

~ 1 6 0 7 2

P R O C E S S F O R T H E F O R M A T I O N OF H U M A N - H U M A N

H Y B R I D O M A

Kimiyosh Tsuji, Nobuna Ikewaki, Yokohama, Japan assigned to Idemitsu Kosan Co Ltd

A human-human hybridoma is formed by sub- jecting a transformed human cell to a pro- liferaton inhibitory treatment and then fusing the thus-treated human cell with a human anti- body producing cell. A desired clone may be selected from the resultant fused cells by using an anti-HLA antibody.

4916073

CDNA AND GENE F O R H U M A N A N G I O G E N I N ( A N G I O G E N E S I S FACTOR) AND M E T H O D S O F

E X P R F ~ S I O N

Bert L Vallee, Kotoku Kurachi assigned to Pre- sident and Fellows of Harvard College

DNA sequences encoding a protein having angiogenic activity are disclosed. Expression vectors containing these sequences are in- troduced into host cells and direct the produc- tion of the angiogenic protein. Proteins produced according to the invention are useful in the diagnosis of malignancies, for promoting wound healing, and for other diagnostic and therapeutic purposes.