PATENT ABSTRACTS

Genetic transformation of a zygote and the em- bryo and mature organism which result there- from is obtained by placing or inserting exogenous genetic material into the nucleus of the zygote or into any genetic material which ul- timately forms at least a part of the nucleus of the zygote. It is preferred that the exogenous genetic material be added to a pronuclei of the zygote and is particularly preferred that it be added to the male pronucleus of the zygote. Thereafter, the zygote is allowed to undergo differentiation and development into the organism. The genotype of the zygote and the organism which results therefrom will include the genotype of the exogenous genetic material and the exogenous genetic material will be phenotypically ex- pressed. The invention can be utilized in a variety of ways including, for example, animal and plant breeding to modify or create new species, it can be used in epigenetics and in the understanding and treatment of genetic diseases.

DNA ENCODING AN ANTIGENIC PROTEIN DERIVED FROM EIMERIA TENELLA AND

VACCINES FOR PREVENTION OF COCCIDIOSIS CAUSED BY

EIMERIA TENELLA

William Andrews, Virginia M Brothers, James G Files, Iren Kuhn, Michael McCaman, Leland Paul, Stacey Sias, Thomas Gore, Karel Z New- man, John L Tedesco assigned to Solvay & Cie S A

A genomic DNA molecule having the nucleic acid sequence set forth in FIG. 1 and encoding an antigenic protein derived from Eimeria tenella has been isolated. The protein has a molecular weight of about 25,000 daltons and is composed of two polypeptides joined by a dis- ulfide bond. One of the polypeptides is charac- terized by a molecular weight of about 17,000 daltons and by a blocked N-terminal amino acid and having the amino acid sequence set forth in FIG. 1. The other polypeptide is characterized by a molecular weight of about 8,000 daltons and has the amino acid sequence set forth in FIG. 1. A cDNA molecule encoding the 25,000 dalton polypeptide with a continuous amino acid sequence has been inserted into expression vectors capable of expressing the 25,000 dalton polypeptide directly or as a fused polypeptide. The polypeptides produced are used in vaccines to immunize chickens against infection by Eimeria tenella.

483

4873195

BLOCK-FORMED BASIDIOMYCETE AND A

METHOD OF CULTIVATION FOR THE SAME

Yoshimasa Kubo, Masao Hondo, Masashi Tanaka, Akihiko Asahi, Suzaka, Japan assigned to Kabushiki Kaisha Akita

The block-formed basidiomycete of the present invention is formed by a block of basidiomy- cetous spawns, and its upper face which is op- posite the side of the root section is a cut face or formed like a surface of cauliflowers or broc- colis. Spawns agglutinate to one another to form a block, preferably, they extend from the root side upward almost in parallel and agglutinate. The method of cultivating the block-formed basidiomycete comprises following steps: (a) Fil- ling media in a cultivation bottle; (b) sterilizing the media; (c) inoculating seed spawns on the media to cultivate spawns; (d) removing part of the seed spawns to form a bed after the cultiva- tion; (e) producing many projected primordia as spawn tissues on the bed by propagating the spawns in a vegetation room after forming the bed; and (f) agglutinating the primorida by plumping them, the primordia being formed by suppressing the production of the fruit bodies under the condition of a shortage of oxygen and a surplus of carbon dioxide gas after producing the primorida.

4874705

4874706

MANUFACTURE AND USE OF TAGETITOXIN

Richard D Durbin, Jean H Lukens, Thomas F Uchytil, Nichols Rhodehamel assigned to Wisconsin Alumni Research Foundation

An exotoxin of the plant pathogen, Pseudo- monas syringae pv. tagetis selectively inhibits the development of chloroplasts in growing plant tissue. The exotoxin, known as tagetitoxin, can be produced efficiently from a mutant high- producing line of the bacteria, designated C42mr2+. The tagetitoxin can also be readily purified from the culture medium of the bacteria.