2014 iowa evaluation of insecticides and plant ...performance of registered and pre-commercial...

IOWA STATE UNIVERSITY OF SCIENCE AND TECHNOLOGY

2014 IOWA EVALUATION OF INSECTICIDES

AND

PLANT-INCORPORATED PROTECTANTS

CORN PESTS RESEARCH PROJECT

DEPARTMENT OF ENTOMOLOGY

AMES, IOWA 50011-3140

SUE BLODGETT, CHAIR

INSECT INVESTIGATED

Corn Rootworm

Diabrotica virgifera virgifera and Diabrotica barberi

Report Authors February 16, 2015

Aaron Gassmann File number: 297-14

Patrick Weber

2014 IOWA EVALUATION OF INSECTICIDES AND

PLANT INCORPORATED PROTECTANTS

TABLE OF CONTENTS Page 1. Research Personnel .......................................... 1 2. Introduction and Objective ............................... 2 3. Testing Procedures and Evaluations ............... 2 4. Results and Discussion ................................... 5 5. Studies Conducted ............................................ 8 6. Corn Rootworm Evaluation Tables Ames (Johnson Farm) Comparison among Multiple Products Root Injury/Percent Consistency ............. 9 Stand Count ............................................ 9 Percent Lodging .................................... 10 Yield ...................................................... 10 Valent Belay 50WDG Efficacy and Yield Root Injury/Percent Consistency ........... 11 Stand Count .......................................... 11 Yield ...................................................... 12 Dow AgroSciences Smartstax study Root Injury/Percent Consistency ........... 13 Stand Count .......................................... 13 Yield ...................................................... 13

Table of Contents (Continued) Page Boone (Bruner Farm) Valent Belay 50WDG Efficacy and Yield Root Injury/Percent Consistency ........... 14 Stand Count .......................................... 14 Yield ...................................................... 15 Nashua Comparison among Multiple Products Root Injury/Percent Consistency ........... 16 Stand Count .......................................... 17 Percent Lodging .................................... 18 Yield ...................................................... 19 Monsanto Traits and Insecticide Study Root Injury/Percent Consistency ........... 20 Stand Count .......................................... 20 Percent Lodging .................................... 21 Yield ...................................................... 21 North Central Iowa (1) Comparison among Bt Rootworm Traits Root Injury/Percent Consistency ........... 22 Stand Count .......................................... 22 Percent Lodging .................................... 23 Yield ...................................................... 23 Syngenta Comparison among Tefluthrin Formulations Root Injury/Percent Consistency ........... 24 Stand Count .......................................... 24 Yield ...................................................... 25 Dow AgroSciences Smartstax Study Root Injury/Percent Consistency ........... 25 Stand Count .......................................... 26 Percent Lodging .................................... 26 Yield ...................................................... 26

Table of Contents (Continued) Page North Central Iowa (2) Comparison among Bt Rootworm Traits Root Injury/Percent Consistency ........... 27 Stand Count .......................................... 27 Percent Lodging .................................... 28 Yield ...................................................... 28 North Central Iowa (3) Comparison among Bt Rootworm Traits Root Injury/Percent Consistency ........... 29 Stand Count .......................................... 29 Yield ...................................................... 30 Dow AgroSciences Smartstax Study Root Injury/Percent Consistency ........... 31 Stand Count .......................................... 31 Yield ...................................................... 31 Monsanto Traits and Insecticide Study Root Injury/Percent Consistency ........... 32 Stand Count .......................................... 32 Yield ...................................................... 33 North Eastern Iowa Comparison among Bt Rootworm Traits Root Injury/Percent Consistency ........... 33 Stand Count .......................................... 34 Percent Lodging .................................... 34 Yield ...................................................... 35 Dow AgroSciences Smartstax Study Root Injury/Percent Consistency ........... 35 Stand Count .......................................... 36 Percent Lodging .................................... 36 Yield ...................................................... 36

Table of Contents (Continued) Page 7. Appendix I - Agronomic Information ............. 37 8. Appendix II – Weather Data ............................ 42 9. Appendix III - Materials Tested ...................... 49

10. Appendix IV - Research Pictures .................. 51

2014 IOWA EVALUATION OF INSECTICIDES AND

PLANT INCORPORATED PROTECTANTS

CORN PESTS RESEARCH PROJECT

DEPARTMENT OF ENTOMOLOGY

AMES, IOWA 50011-3140

Project Leader: Aaron Gassmann

Agricultural Specialist: Patrick Weber

Postdoctoral Research Associate: John Doudna Siva Jakka Ram Shrestha Graduate Research Assistants: Eric Clifton Mike Dunbar David Ingber Kenneth Masloski Aubrey Paolino Coy St. Clair Research Associate: Sean Bradley Technical Assistants: Alex Abdallah Anthony Blake Andrew Guiness Cory Hermsen Andrea Raygor Eric Stuart University Research Farm Superintendents: Kent Berns Mike Fiscus Bernard Havlovic Kenneth Pecinovsky Myron Rees Joshua Sievers

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INTRODUCTION

In Iowa, the species of corn rootworm (CRW) include western corn rootworm Diabrotica virgifera virfigera LeConte and northern corn rootworm D. barberi Smith and Lawrence, and these two species are the most damaging pests of corn Zea mays in the Unites States Corn Belt. Eggs are laid in the soil during the fall and hatch the following spring. Larval feeding on corn roots in June may diminish yield by reducing plant growth and drought tolerance and by imposing harvest losses due to plant lodging. Adult emergence from the soil is underway by early July and continues through late summer. Additional crop losses may be caused by adult beetles feeding on the corn silk and on soft doughy kernels. In Iowa, crop rotation, where it fits cropping practices, remains the preferred method of management. However, it is also economically feasible to protect corn roots with soil–applied insecticides and plant-incorporated protectants, which are transgenic corn hybrids that produce insecticidal toxins derived from Bacillus thuringiensis (Bt). In additional to corn rootworm, several aboveground lepidopteran pests feed on corn in Iowa. These include the European corn borer Ostrinia nubilalis, black cutworm Agrotis ipsilon, fall armyworm Spodoptera frugiperda, and corn earworm Helicoverpa zea. Over the last decade, Bt corn varieties have been made commercially available to protect corn from these pests.

OBJECTIVE

The goal of this research program is to serve the agricultural community of Iowa by monitoring and evaluating the performance of registered and pre-

commercial insecticides and transgenic corn hybrids. To achieve this goal, we maintain a viable, proactive, progressive and scientifically sound product evaluation program.

TESTING PROCEDURES AND EVALUATIONS

Field Sites: Research plots were established at seven Iowa locations in 2014. Three research locations were on Iowa State University farms: 1) Johnson Farm (Ames), 2) Bruner Farm (Boone), 3) Northeast Research and Demonstration Farm (Nashua). Four research locations were commercial fields in north central Iowa (3 locations) and northeast Iowa (1 location). At the Johnson Farm, Northeast Iowa Research and Demonstration Farm, two sections are rotated annually as either research location or late-planted trap crop. The seed planted for the trap crop is a mixed maturity blend with a greater proportion of late-maturing varieties. This trap crop constitutes a favorable environment for adult female CRW late in the season when other fields are maturing. All other research locations had a history of continuous corn production, which is conducive to a high abundance of CRW. Table 1 lists the studies conducted at each location.

METHODS

Field plot design: Unless stated otherwise, the experimental design was a randomized complete block with four replications. Treatments in studies comparing multiple products at the ISU Johnson Farm, Ames Iowa (Tables 2-5) and the Northeast Iowa Research and Demonstration Farm, Nashua Iowa (Tables 15-18) were paired rows 75 feet

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in length. Treatments in the Monsanto Traits and Insecticides studies at the Northeast Iowa Research and Demonstration Farm, Nashua Iowa (Tables 19-22) and North Central Iowa (3) (Tables 44-46) were paired rows 25 feet in length. All other studies were four row treatments with 25 feet per row.

Planting: Seeds were pre-bagged and planted with a four-row John Deere Max EmergeTM 7100 integral planter that had 30 inch row spacings. The standard planter fiberglass seed hoppers with attached finger-pickup mechanism were replaced with modified units. On the new units, the metal plate that covered the fingers had been replaced with a 7/8-inch clear Plexiglas plate. Inserted through the Plexiglas was a small stainless steel cylinder. The cylinder was positioned to deliver seed to the pickup fingers. At the beginning of each replication, pre-bagged seeds were dumped into the steel cylinder. At the beginning of each replication, a hydraulic motor (attached to the planter’s drive shaft) was activated to deliver seed immediately into the ground. At the end of each replication, this same hydraulic motor was activated to expel any unplanted seed, and the finger pickup mechanism was visually inspected through the clear Plexiglas plate to ensure that no seed was present.

Plant-incorporated protectants: Plant-incorporated protectants were evaluated in corn hybrids producing insecticidal toxins derived from the bacterium Bacillus thuringiensis (Bt), and these are described in Appendix III. Seed treatments: All the DeKalb hybrids were treated with Poncho 500.

All the Mycogen hybrids were treated with Cruiser Extreme® 250. All the Syngenta hybrids were treated with Cruiser 250 or 1250. All the Pioneer hybrids were treated with either Cruiser 250 + Maxim Quattro + Poncho1250/Votivo + PPCT12/13 or Cruiser 250 + Maxim Quattro + PPCT2013. These seed treatments target secondary pests, such as wireworm and seed corn maggot but may have some efficacy against CRW at levels higher than 250 mg active ingredient per kernel. Granular soil-applied insecticides: The granular insecticides Aztec 2.1G, Belay 50WDG and Force 3.0G were applied with modified Noble® metering units mounted on the planter. The Noble units were calibrated in the laboratory to accurately deliver material at a tractor speed of 4 mph. The Aztec 2.1G and Belay 50WDG insecticides were applied with in-furrow placement and the Force 3.0G insecticide was applied with T-band placement.

Liquid soil-applied insecticides: The liquid products Capture LFR 1.5FL was applied in-furrow and Force 250CS insecticide was applied T-Band at planting with a compressed-air system built directly into the planter by Almaco manufacturing (Nevada, IA). Capture LFR and Force CS were applied as ounces per 1000 row feet using Teejet XR80015EVS spray nozzles at 21 psi to deliver 5 GPA of finished spray at a tractor speed of 4 mph. The liquid product Capture LFR 1.5FL was mixed with Starter Fertilizer (10-34-0 NPK) while Force CS used water as the carrier.

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SmartBoxTM application of soil-applied insecticides: The Aztec-SB 4.67G, Counter-SB 20G, and SmartChoice-SB 5G, insecticide treatments were applied with modified SmartBoxTM metering units mounted on the planter. The commercial SmartBoxTM units were removed from their large-base containers and sandwiched between a flat metal plate on the bottom and a custom-made, threaded plastic cap on the top. The bottom plate had been fabricated so that it could slide in and out of the same planter mounting brackets used for the noble units. An inverted 1-liter Nalgene bottle attached to the top provided a secure and sealed container for insecticide for the SmartBoxTM units. Clear plastic tubes directed the granular insecticides to both the in-furrow and T-band placement. One controller mounted in the tractor cab was used to operate all the SmartBoxTM metering units. These units were set-up for proper applications and managed using the AMVAC SmartBox System Manager. All treatments were applied at 4 mph using the “fixed speed mode” on the SmartBoxTM controllers. These SmartBoxTM metering units were accurately calibrated before going to the field to deliver ounces of product per 1000 row feet.

General methodology: For all applications of soil-applied insecticides, 11-inch poly-bristle skirts were attached to a frame and positioned so the bristle tips touched the ground. Each row was constantly monitored to ensure that insecticides were applied correctly. Final incorporation was accomplished with drag chains mounted behind the closing wheels.

Evaluations

Stand counts: The number of plants in 17.5 row-ft was recorded. This was done early and late in the growing season and then averaged. Root injury: After the majority of corn rootworms had finished feeding, roots were dug to assess feeding injury. This took place from July 24, 2014 through August 20, 2014. Prior to leaving the field, excess soil was removed from roots and all roots were labeled with study name, plot number and row number using a permanent marker. Roots were transported to the Insectary Building at Iowa State University where they were soaked in water and then washed with a pressurized hose to remove any remaining soil. Roots were evaluated for rootworm feeding injury following the Iowa State Node-Injury Scale (0-3): Node-Injury Scale (0-3): 0.00 - No feeding damage (lowest rating

that can be given). 1.0 - One node (circle of roots), or the

equivalent of an entire node, pruned to within 1.5 inches of the stalk or soil line.

2.00 - Two nodes pruned.

3.00 - Three or more nodes pruned. (Highest rating that can be given)

Damage in between complete nodes pruned is noted as the percentage of the node missing (e.g., 1.50 = one and a half nodes pruned and 0.25 = one quarter of one node pruned).

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The linear node-injury scale allows injury to be expressed intuitively and has proved useful in evaluating minor injury. For an interactive guide to the Node-injury scale, see the Iowa State University Entomology web site at: http://www.ent.iastate.edu/dept/faculty/gassmann/rootworm

Product consistency: Percent product consistency was calculated as the percentage of times a treatment limited feeding injury to 0.25 node or less (greater injury can result in economic yield loss, especially when plants are moisture stressed).

Lodging counts: A plant was considered lodged if it was leaning at least 30 degrees from vertical. These data were taken at harvest time along with final stand count numbers.

Yields: Studies that were taken to yield were machine harvested with either a modified John Deere 9450 plot combine (Iowa State University research farms only), Kincade (Massey Ferguson) #8xp, or Gleaner plot combine. Weight (pounds) and percent moisture were recorded from either a HarvestMaster brand or Shivvers brand 5010 moisture meter on harvest data collection system. These measurements were converted to bushels per acre of No. 2 shelled corn

(56 lbs. per bushel) at 15.5% moisture in Microsoft Excel.

All data were analyzed with analysis of variance (ANOVA) procedures using SAS 9.3. When a significant treatment effect was present pairwise comparisons made among means with an experimentwise error rate of P < 0.05.

CALIBRATION INFORMATION

All Noble® units were laboratory calibrated and units were spot-checked in the field prior to planting. SmartBoxTM units were calibrated on the planter in accordance with instructions in the SmartBox Operator’s Manual. During calibration and planting, the flowability of each formulation was noted, as well as any other calibration problems. There were no calibration or delivery problems with any treatment.

RESULTS AND DISCUSSION

Because of below average winter temperatures and wet soils early in the growing season, mortality of rootworm eggs and larvae was greater than normal, and this resulted in low pest pressure at many study locations.

Rootworm pressure was very light at Johnson Research Farm and Bruner Research Farm, and few differences existed among treatments. However, a significant reduction in injury was found for SmartStax compared to the untreated check (Tables 6, 9 and 12). Additionally, pest pressure was very low at North Central Iowa (3) and no meaningful differences were observed among treatments (Tables 38, 41 and 44).

Rootworm pressure at the Northeast Iowa Research and

http://www.ent.iastate.edu/dept/faculty/gassmann/rootworm

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Demonstration Farm was moderate to heavy, with the untreated checks displaying between 1.6 to 1.9 nodes of root injury in one study (Table 15). Past research at this farm has found resistance by western corn rootworm to Bt toxin Cry3Bb1, which is the rootworm active Bt toxin in YieldGard VT3. Past research has also found cross-resistance between Cry3Bb1 and mCry3A, which is the rootworm active Bt toxin in Agrisure 3000GT. The presence of resistance to Cry3Bb1 and mCry3A is reflected in the root injury observed in this study. Injury to 3000GT was 1.6 nodes and did not differ from the untreated checks (Table 15). Similarly, injury to VT3 was significantly greater than all other rootworm treatments (Table 15). To date, no resistance to Cry34/35Ab1 has been found at the Northeast Iowa Research and Demonstration Farm and this Bt toxin is present in Pioneer OAM1 and Agrisure 3122. In general, similar performance was observed among non-rootworm Bt corn with Aztec, OAM1 and 3122. In some cases, combining soil-applied insecticide with Bt corn reduced injury, but in other instances, this was not observed. Among rootworm treatments, yields were lowest for YieldGard VT3 and Agrisure 3000GT and these did not differ from the untreated checks (Table 18). These reductions in yield were likely the result of high levels of root injury caused by the presence of resistance to mCry3A and Cry3Bb1.

Similarly, in a second study at the Northeast Iowa Research and Demonstration Farm, no differences were observed between VT3 and untreated checks for root injury, with all hybrids displaying greater than 0.75

nodes of injury (Table 19). By contrast, all other rootworm treatments including rootworm Bt corn (e.g., SmartStax and Agrisure 3122), non-rootworm Bt corn with soil-applied insecticide, and rootworm Bt corn with soil-applied insecticide did not differ significantly, and all displayed less than 0.25 nodes of root injury.

North Central Iowa (1) and North Central Iowa (2) were selected as research sites because of high levels of injury to Cry3Bb1 corn during the previous growing season. Subsequent laboratory bioassays confirmed the presence of Cry3Bb1 resistance in these fields.

North Central Iowa (1) had modest to heavy pressure, with all untreated checks displaying 1.5 or greater nodes of root injury. With the exception of VT3, all products reduced injury to below 0.50 nodes (Table 23). In general either non-rootworm Bt corn with Aztec or Bt corn containing Cry34/35Ab1 (found in both SmarStax and Herculex) provided adequate protection of roots (Table 23 and 30). In another study at this location, we found similar levels of root protection among several soil-applied insecticides on non-rootworm Bt corn, and all products significantly reduced injury compared to the untreated check (Table 27).

A similar pattern was observed at North Central Iowa (2) (Table 34), with no difference in root injury observed between VT3 and the untreated check. However, all other rootworm treatments significantly reduced feeding injury. Both HXX and SmartStax reduced injury below 0.25 nodes (Table 34).

Rootworm pressure was modest at the North Eastern Iowa location, with untreated checks ranging from 0.60 to

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0.85 (Table 47). All corn rootworm treatments reduced injury below 0.25 nodes and all were significantly different from the untreated check, although not from each other (Table 47).

AGRONOMIC INFORMATION, WEATHER DATA AND MATERIALS

TESTED AND PICTURES OF RESEARCH ACTIVITIES

Agronomic information and field history are listed in Appendix I. Weather data from the test site or the nearest Iowa climatological station are listed in Appendix II. Information on materials tested is listed in Appendix III. Pictures of various research activities are in Appendix IV.

RESEARCH SUPPORT

Support for this research was provided by the Iowa Agriculture and Home Economics Experiment Station and by the following companies: AMVAC, Dow AgroSciences, DuPont-Pioneer, FMC, Monsanto, Syngenta and Valent.

WAIVER OF ENDORSEMENT

This report deals with the relative ability of each treatment to protect corn from damage by soil insects. This information is not presented to endorse the use of any product and the name of Iowa State University should not appear in any advertising without prior written consent. Iowa State University, their respective officers, agents, or employees, have not made, and do not hereby make, any representation, warranty or covenant with respect to the use of these test results, nor will they be liable for any damages, losses, or claims, including those of an incidental or consequential

nature, arising out of the use of these test results.

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Table 1. Iowa evaluation of insecticides and plant-incorporated protectants.

1 All studies were conducted in either a continuous cornfield or following corn rootworm trap crop, to ensure the presence of rootworm larvae in the study.

Experimental Unit Size

Table Entries/ Row # Test Location Type of Studies1 Numbers Test Length (ft) Reps

Ames-Johnson Farm 1)Comparison among

Multiple Products. 2)Valet Belay 50WDG

Efficacy and Yield 3)Dow AgroSciences

Smartstax Study

2-5 6-8

9-11

10

10

2

75 25

25

4 4 4

Boone-Bruner Farm 1)Valent Belay 50WDG Efficacy and Yield

12-14 10 25 4

Nashua 1)Comparison among Multiple Products 2)Monsanto Traits and

Insecticides

15-18 19-22

15

12

75

25

4

4

North Central Iowa (1)

1)Comparison among Bt Rootworm Traits

2)Syngenta Comparison among Tefluthrin Formulations

3)Dow AgroSciences Smartstax

23-26 27-29

30-33

10

7

2

25

25

25

6 4

4

North Central Iowa (2) 1)Comparison among Bt Rootworm Traits

34-37 10 25 6

North Central Iowa (3)


2)Dow AgroSciences Smartstax Study

3)Monsanto Traits and Insecticides

38-40 41-43

44-46

10

2

12

25 25 25

6

4

4

North Eastern Iowa


2)Dow AgroSciences Smartstax

47-50

51-54

10

2

25

25

6 4

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Table 2. Node injury and product consistency for Comparison among Multiple Products: Johnson Farm, Ames1

Node- Product

Treatment2 Form. Rate3 Placement4 Injury 5,6,7 Consistency8,9

Pioneer OAM1 ------- ------- ------- 0.01a 100a DeKalb VT3 + Aztec-SB 4.67G 0.14 SB/Furrow 0.02a 100a Agrisure 3122 RIB + Counter-SB 20G 0.90 SB/Furrow 0.02a 100a Pioneer OAM1 + SmartChoice-SB 5G 0.25 SB/Furrow 0.03a 100a Agrisure 3122 RIB ------- ------- ------- 0.05a 100a DeKalb non-RW Bt + Aztec 2.1G 0.14 Furrow 0.20ab 85ab DeKalb VT3 ------- ------- ------- 0.20ab 75ab DeKalb non-RW Bt ------- ------- ------- 0.24ab 80ab Agrisure non-RW Bt ------- ------- ------- 0.57 b 60ab Pioneer non-RW Bt ------- ------- ------- 1.15 c 35 b

1 Planted May 19, 2014; evaluated August 5, 2014 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb VT3 = YieldGard VT Triple (DKC58-83); DeKalb-non-RW Bt = DeKalb brand VT2PRO Isoline (DKC 59-89); Pioneer OAM1 = Pioneer Optimum AcreMax1 (P0533AM1); Pioneer non-RW Bt = Pioneer Herculex 1 (P0533HR); Agrisure non-RW Bt = Syngenta Agrisure GT (Agrisure N53-W3, Glyphosate Tolerant); Agrisure 3122 RIB = Syngenta Agrisure RIB (Agrisure N52W-3122 RIB)

3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow = insecticide applied at planting time; SB = SmartBox application at planting time 5 Chemical and check means based on 20 observations (5 roots/2 rows x 4 replications) 6 Iowa State Node-Injury scale (0-3). Number of full or partial nodes completely eaten 7 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 8 Product consistency = Percentage of times nodal injury was 0.25 (¼ node eaten) or less

9 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) Table 3. Stand counts for Comparison among Multiple Products: Johnson Farm, Ames1

Stand

Treatment2 Form. Rate3 Placement4 Count5,6

DeKalb VT3 ------- ------- ------- 38.00a DeKalb VT3 + Aztec-SB 4.67G 0.14 SB/Furrow 37.25ab DeKalb non-RW Bt + Aztec 2.1G 0.14 Furrow 36.75abc DeKalb non-RW Bt ------- ------- ------- 36.50abc Pioneer OAM1 + SmartChoice-SB 5G 0.25 SB/Furrow 36.00abc Pioneer non-RW Bt ------- ------- ------- 36.00abc Pioneer OAM1 ------- ------- ------- 35.75abc Agrisure 3122 RIB ------- ------- ------- 34.75abc Agrisure non-RW Bt ------- ------- ------- 34.50 bc Agrisure 3122 RIB + Counter-SB 20G 0.90 SB/Furrow 33.75 c

1 Planted May 19, 2014; evaluated June 6 and September 23, 2014 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb VT3 = YieldGard VT Triple (DKC58-83); DeKalb-non-RW Bt = DeKalb brand VT2PRO Isoline (DKC 59-89); Pioneer OAM1 = Pioneer Optimum AcreMax1 (P0533AM1); Pioneer non-RW Bt = Pioneer Herculex 1 (P0533HR); Agrisure non-RW Bt = Syngenta Agrisure GT (Agrisure N53-W3, Glyphosate Tolerant); Agrisure 3122 RIB = Syngenta Agrisure RIB (Agrisure N52W-3122 RIB)

3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow = insecticide applied at planting time; SB = SmartBox application at planting time 5 Chemical and check means based on 16 observations (2-row treatment x 17.5 row-feet/treatment x 4 replications x 2 evaluation dates) 6 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)

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Table 4. Lodging for Comparison among Multiple Products: Johnson Farm, Ames1

Treatment2 Form. Rate3 Placement4 Lodging5,6

DeKalb non-RW Bt ------- ------- ------- 0 DeKalb non-RW Bt + Aztec 2.1G 0.14 Furrow 0 DeKalb VT3 ------- ------- ------- 0 DeKalb VT3 + Aztec-SB 4.67G 0.14 SB/Furrow 0 Agrisure 3122 RIB ------- ------- ------- 0 Agrisure 3122 RIB + Counter-SB 20G 0.90 SB/Furrow 0 Pioneer OAM1 ------- ------- ------- 0 Pioneer OAM1 + SmartChoice-SB 5G 0.25 SB/Furrow 0 Pioneer non-RW Bt ------- ------- ------- 6 Agrisure non-RW Bt ------- ------- ------- 11

1 Planted May 19, 2014; evaluated September 23, 2014 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb VT3 = YieldGard VT Triple (DKC58-83); DeKalb-non-RW Bt = DeKalb brand VT2PRO Isoline (DKC 59-89); Pioneer OAM1 = Pioneer Optimum AcreMax1 (P0533AM1); Pioneer non-RW Bt = Pioneer Herculex 1 (P0533HR); Agrisure non-RW Bt = Syngenta Agrisure GT (Agrisure N53-W3, Glyphosate Tolerant); Agrisure 3122 RIB = Syngenta Agrisure RIB (Agrisure N52W-3122 RIB)

3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow = insecticide applied at planting time; SB = SmartBox application at planting time 5 Chemical and check means based on 8 observations (2-row treatment x 17.5 row-feet/treatment x 4 replications) 6 No significant differences between means (ANOVA, P < 0.05) Table 5. Yield for Comparison among Multiple Products: Johnson Farm, Ames1

Bushels/

Treatment2 Form. Rate3 Placement4 Acre5,6,7

DeKalb non-RW Bt + Aztec 2.1G 0.14 Furrow 203a DeKalb VT3 ------- ------- ------- 189a DeKalb non-RW Bt ------- ------- ------- 174ab Agrisure 3122 RIB ------- ------- ------- 172ab Pioneer OAM1 + SmartChoice-SB 5G 0.25 SB/Furrow 168ab DeKalb VT3 + Aztec-SB 4.67G 0.14 SB/Furrow 146ab Pioneer non-RW Bt ------- ------- ------- 139ab Agrisure non-RW Bt ------- ------- ------- 133ab Agrisure 3122 RIB + Counter-SB 20G 0.90 SB/Furrow 124ab Pioneer OAM1 ------- ------- ------- 105 b

1 Planted May 19, 2014; machine harvested October 28, 2014 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb VT3 = YieldGard VT Triple (DKC58-83); DeKalb-non-RW Bt = DeKalb brand VT2PRO Isoline (DKC 59-89); Pioneer OAM1 = Pioneer Optimum AcreMax1 (P0533AM1); Pioneer non-RW Bt = Pioneer Herculex 1 (P0533HR); Agrisure non-RW Bt = Syngenta Agrisure GT (Agrisure N53-W3, Glyphosate Tolerant); Agrisure 3122 RIB = Syngenta Agrisure RIB (Agrisure N52W-3122 RIB)

3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow = insecticide applied at planting time; SB = SmartBox application at planting time 5 Chemical and check means based on 4 observations (2-row treatment x 68 row-feet/treatment x 4 replications) 6 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 7 Yields converted to15.5% Moisture

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Table 6. Average root injury and product consistency for the Valent Belay 50WDG Efficacy and Yield: Johnson Farm,

Ames1

Node- Product

Treatment2 Form. Rate3 Placement4 Injury5,6,7 Consistency8,9

DeKalb SSTX RIB + Belay 50WDG 0.15 Furrow 0.01a 100 DeKalb SSTX RIB + Belay 50WDG 0.20 Furrow 0.01a 100 DeKalb SSTX RIB + Force 250CS 0.57 Furrow 0.01a 100 DeKalb SSTX ------ ------ ------ 0.02a 100 DeKalb non-RW Bt + Force 250CS 0.57 Furrow 0.02ab 100 DeKalb SSTX RIB + Belay 50WDG 0.10 Furrow 0.02ab 100 DeKalb non-RW Bt + Belay 50WDG 0.15 Furrow 0.04ab 95 DeKalb non-RW Bt + Belay 50WDG 0.20 Furrow 0.04ab 95 DeKalb non-RW Bt + Belay 50WDG 0.10 Furrow 0.08 bc 95 DeKalb non-RW Bt ------ ------ ------ 0.10 c 95

1 Planted May 30, 2014; evaluated August 19, 2014

2 Non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-RW Bt = DeKalb brand RR2 Isoline (DKC 62-95); DeKalb SSTX RIB = DeKalb brand Smartstax RIB (DKC 62-08) 3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow = Insecticide applied at planting time

5 Chemical and check means based on 20 observations (5 roots/2 rows x 4 replications) 6 Iowa State Node-Injury Scale (0-3). Number of full or partial nodes completely eaten 7 Chemical and check means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 8 Product consistency = Percentage of times nodal injury was 0.25 (¼ node eaten) or less 9. No significant differences between means (ANOVA, P < 0.05) Table 7. Stand counts for the Valent Belay 50WDG Efficacy and Yield: Johnson Farm, Ames1

Stand

Treatment2 Form. Rate3 Placement4 Counts5,6

DeKalb SSTX ------ ------ ------ 32.25a DeKalb non-RW Bt + Belay 50WDG 0.10 Furrow 31.50a DeKalb non-RW Bt + Belay 50WDG 0.20 Furrow 31.50a DeKalb non-RW Bt + Belay 50WDG 0.15 Furrow 31.00a DeKalb SSTX RIB + Force 250CS 0.57 Furrow 30.25ab DeKalb non-RW Bt + Force 250CS 0.57 Furrow 30.00ab DeKalb SSTX RIB + Belay 50WDG 0.10 Furrow 29.75ab DeKalb SSTX RIB + Belay 50WDG 0.15 Furrow 29.75ab DeKalb SSTX RIB + Belay 50WDG 0.20 Furrow 28.25ab

DeKalb non-RW Bt ------ ------ ------ 26.50 b

1 Planted May 30, 2014; evaluated June 10 and September 25, 2014 2 Non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-RW Bt = DeKalb brand RR2 Isoline (DKC 62-95); DeKalb SSTX RIB = DeKalb brand Smartstax RIB (DKC 62-08) 3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow = Insecticide applied at planting time

5 Chemical and check means based on 16 observations (2 rows/ treatment x 17.5 row-ft/treatment x 4 replications x 2 evaluations)

6 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)

12

Table 8. Average yields for the Valet Belay 50WDG Efficacy and Yield: Johnson Farm, Ames1

Bushels/


DeKalb SSTX ------- ------- ------- 214 DeKalb non-RW Bt ------- ------- ------- 2128

DeKalb non-RW Bt + Force 250CS 0.57 Furrow 198 DeKalb non-RW Bt + Belay 50WDG 0.10 Furrow 194 DeKalb SSTX RIB + Belay 50WDG 0.20 Furrow 193 DeKalb SSTX RIB + Belay 50WDG 0.10 Furrow 189 DeKalb SSTX RIB + Belay 50WDG 0.15 Furrow 188 DeKalb SSTX RIB + Force 250CS 0.57 Furrow 186 DeKalb non-RW Bt + Belay 50WDG 0.15 Furrow 176 DeKalb non-RW Bt + Belay 50WDG 0.20 Furrow 168

1 Planted May 30, 2014; machine harvested October 28, 2014 2 Non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-RW Bt = DeKalb brand RR2 Isoline (DKC 62-95); DeKalb SSTX RIB = DeKalb brand Smartstax RIB (DKC 62-08) 3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow = Insecticide applied at planting time

5 Chemical and check means based on 4 observations (4-rows/ treatment x 19 row-feet/treatment x 4replications) 6 No significant differences between means (ANOVA, P < 0.05) 7 Yields converted to 15.5% Moisture 8 Mean based on 3 observations (4-rows/ treatment x 19 row-feet/treatment x 3 replications)

13

Table 9. Average root injury and product consistency for the Dow AgroSciences Smartstax Study: Johnson Farm, Ames1

Node- Product

Treatment2 Injury3,4,5 Consistency6,7

Mycogen Smartstax 0.03a 100a Mycogen non-RW Bt 1.05 b 25 b


2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; Mycogen non-RW Bt = Mycogen brand RR (Mycogen 2K591); Mycogen Smartstax = Mycogen brand Smartstax (Mycogen 2K594)

3 Chemical and check means based on 20 observations (5 roots/2 rows x 4 replications) 4 Iowa State Node-Injury Scale (0-3). Number of full or partial nodes completely eaten 5 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 6 Product consistency = Percentage of times nodal injury was 0.25 (¼ node eaten) or less 7 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) Table 10. Stand counts for the Dow AgroSciences Smartstax Study: Johnson Farm, Ames1

Stand

Treatment2 . Counts3,4

Mycogen non-RW Bt 32.50 Mycogen Smartstax 31.50

1 Planted May 20, 2014; evaluated June 9 and September 23, 2014 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; Mycogen non-RW Bt = Mycogen brand RR (Mycogen 2K591); Mycogen Smartstax = Mycogen brand Smartstax (Mycogen 2K594)

3 Means based on 16 observations (2 rows/ treatment x 17.5 row-ft/treatment x 4 replications x 2 evaluation dates) 4 No significant differences between means (ANOVA, P < 0.05) Table 11. Average yield for the Dow AgroSciences Smartstax Study: Johnson Farm, Ames1

Bushels/

Treatment2 Acre3,4,5

Mycogen Smartstax 218 Mycogen non-RW Bt 204

1 Planted May 20, 2014; machine harvested October 28, 2014 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; Mycogen non-RW Bt = Mycogen brand RR (Mycogen 2K591); Mycogen Smartstax = Mycogen brand Smartstax (Mycogen 2K594)

3 Means based on 4 observations (4 rows/ treatment x 19 row-feet/treatment x 4 replications) 4 No significant differences between means (ANOVA, P < 0.05) 5 Yields converted to 15.5% Moisture

14

Table 12. Average root injury and product consistency for the Valent Belay 50WDG Efficacy and Yield: Bruner Farm, Boone.1

Node- Product


DeKalb SSTX RIB + Belay 50WDG 0.15 Furrow 0.03a 100 DeKalb SSTX RIB + Force 250CS 0.57 Furrow 0.04a 95 DeKalb SSTX RIB + Belay 50WDG 0.10 Furrow 0.05a 95 DeKalb SSTX ------- ------- ------- 0.08ab 95 DeKalb non-RW Bt + Belay 50WDG 0.20 Furrow 0.11ab 75 DeKalb SSTX RIB + Belay 50WDG 0.20 Furrow 0.14ab 90 DeKalb non-RW Bt + Force 250CS 0.57 Furrow 0.17ab 90 DeKalb non-RW Bt + Belay 50WDG 0.15 Furrow 0.21ab 65 DeKalb non-RW Bt + Belay 50WDG 0.10 Furrow 0.25 b 65 DeKalb non-RW Bt ------- ------- ------- 0.56 c 45


2 Non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-RW Bt = DeKalb brand RR2 Isoline (DKC 62-95); DeKalb SSTX RIB = DeKalb brand Smartstax RIB (DKC 62-08) 3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow = Insecticide applied at planting time

5 Chemical and check means based on 20 observations (5 roots/2 rows x 4 replications) 6 Iowa State Node-Injury Scale (0-3). Number of full or partial nodes completely eaten 7 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 8 Product consistency = Percentage of times nodal injury was 0.25 (¼ node eaten) or less 9. No significant differences between means (ANOVA, P < 0.05) Table 13. Stand counts for the Valent Belay 50WDG Efficacy and Yield: Bruner Farm, Boone.1

Stand


DeKalb non-RW Bt + Belay 50WDG 0.20 Furrow 33.50a DeKalb SSTX RIB + Belay 50WDG 0.10 Furrow 32.75a DeKalb SSTX RIB + Belay 50WDG 0.15 Furrow 32.50ab DeKalb non-RW Bt + Force 250CS 0.57 Furrow 32.25ab DeKalb non-RW Bt + Belay 50WDG 0.10 Furrow 32.00ab DeKalb non-RW Bt + Belay 50WDG 0.15 Furrow 31.50ab DeKalb SSTX ------ ------ ------ 31.25ab DeKalb SSTX RIB + Belay 50WDG 0.20 Furrow 31.25ab DeKalb SSTX RIB + Force 250CS 0.57 Furrow 27.75 bc DeKalb non-RW Bt ------ ------ ------ 25.00 c

1 Planted May 29, 2014; evaluated June 10 and September 29, 2014 2 Non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-RW Bt = DeKalb brand RR2 Isoline (DKC 62-95); DeKalb SSTX RIB = DeKalb brand Smartstax RIB (DKC 62-08) 3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow = Insecticide applied at planting time

5 Chemical and check means based on 16 observations (2 rows/ treatment x 17.5 row-ft/treatment x 4 replications x 2 evaluations) 6 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)

15

Table 14. Average yields for the Valent Belay 50WDG Efficacy and Yield: Bruner Farm, Boone.1

Bushels/


DeKalb SSTX ------ ------ ------ 196 DeKalb SSTX RIB + Belay 50WDG 0.20 Furrow 195 DeKalb SSTX RIB + Belay 50WDG 0.10 Furrow 185 DeKalb SSTX RIB + Belay 50WDG 0.15 Furrow 182 DeKalb non-RW Bt + Belay 50WDG 0.15 Furrow 181 DeKalb non-RW Bt + Force 250CS 0.57 Furrow 179 DeKalb non-RW Bt + Belay 50WDG 0.20 Furrow 171 DeKalb non-RW Bt + Belay 50WDG 0.10 Furrow 168 DeKalb non-RW Bt ------ ------ ------ 164 DeKalb SSTX RIB + Force 250CS 0.57 Furrow 152

1 Planted May 29, 2014; machine harvested October 31, 2014 2 Non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-RW Bt = DeKalb brand RR2 Isoline (DKC 62-95); DeKalb SSTX RIB = DeKalb brand Smartstax RIB (DKC 62-08) 3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow = Insecticide applied at planting time

5 Chemical and check means based on 4 observations (4-rows/ treatment x 19 row-feet/treatment x 4 replications) 6 No significant differences between means (ANOVA, P < 0.05) 7 Yields converted to 15.5% Moisture

16

Table 15. Root injury and product consistency for Comparison among Multiple Products: Northeast Iowa Research and Demonstration Farm, Nashua1

Node- Product

Treatment2 Form. Rate3 Placement4 Injury 5,6,7 Consistency8,9

Pioneer OAM1 + SmartChoice-SB 5G 0.25 SB/Furrow 0.05a 95a Agrisure 3122 RIB + Force 250CS 0.57 T-Band 0.06a 100a Agrisure 3122 RIB + Counter-SB 20G 0.90 SB/Furrow 0.07ab 95a DeKalb VT3 + Aztec-SB 4.67G 0.14 SB/Furrow 0.21ab 80ab Agrisure 3000GT + Force 250CS 0.57 T-Band 0.32 bc 75ab Agrisure 3000GT + Capture LFR 1.5SC 0.10 Furrow 0.33 c 55ab DeKalb non-RW Bt + Aztec 2.1G 0.14 Furrow 0.38 c 50ab Agrisure 3122 RIB ------- ------- ------- 0.40 c 73ab Pioneer OAM1 ------- ------- ------- 0.48 c 45ab DeKalb VT3 ------- ------- ------- 0.77 d 30 bc Pioneer non-RW Bt ------- ------- ------- 1.66 e 0 c Agrisure 3000GT ------- ------- ------- 1.67 e 0 c DeKalb non-RW Bt ------- ------- ------- 1.77 e 0 c Agrisure non-RW Bt ------- ------- ------- 1.90 e 0 c

1 Planted May 7, 2014; evaluated July 29, 2014 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb VT3 = YieldGard VT Triple (DKC58-83); DeKalb-non-RW Bt = DeKalb brand VT2PRO Isoline (DKC 59-89); Pioneer OAM1 = Pioneer Optimum AcreMax1 (P0533AM1); Pioneer non-RW Bt = Pioneer Herculex 1 (P0533HR); Agrisure non-RW Bt = Syngenta Agrisure GT (Agrisure N53-W3, Glyphosate Tolerant); Agrisure 3122 RIB = Syngenta Agrisure RIB (Agrisure N52W-3122 RIB);

Agrisure 3000GT = Syngenta Agrisure Viptera (Agrisure N53W-3000GT) 3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow & T-Band = insecticide applied at planting time; SB = SmartBox application at planting time 5 Chemical and check means based on 20 observations (5 roots/2 rows x 4 replications) 6 Iowa State Node-Injury scale (0-3). Number of full or partial nodes completely eaten 7 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 8 Product consistency = Percentage of times nodal injury was 0.25 (¼ node eaten) or less 9 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)

17

Table 16. Stand counts for Comparison among Multiple Products: Northeast Iowa Research and Demonstration

Farm, Nashua1

Stand

Treatment2 Form. Rate3 Placement4 Count5,6

Agrisure 3122 RIB + Force 250CS 0.57 T-Band 34.00a Agrisure 3122 RIB ------- ------- ------- 33.00ab Agrisure 3122 RIB + Counter-SB 20G 0.90 SB/Furrow 32.50ab Agrisure 3000GT ------- ------- ------- 32.50ab Agrisure 3000GT + Force 250CS 0.57 T-Band 32.00ab DeKalb non-RW Bt ------- ------- ------- 31.75qb DeKalb non-RW Bt + Aztec 2.1G 0.14 Furrow 31.75ab DeKalb VT3 ------- ------- ------- 31.50ab Pioneer OAM1 ------- ------- ------- 31.25ab Agrisure 3000GT + Capture LFR 1.5SC 0.10 Furrow 31.25ab DeKalb VT3 + Aztec-SB 4.67G 0.14 SB/Furrow 30.75ab Pioneer OAM1 + SmartChoice-SB 5G 0.25 SB/Furrow 30.50ab Agrisure non-RW Bt ------- ------- ------- 29.75ab Pioneer non-RW Bt ------- ------- ------- 29.50ab

1 Planted May 7, 2014; evaluated June 6 and October 9, 2014 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb VT3 = YieldGard VT Triple (DKC58-83); DeKalb-non-RW Bt = DeKalb brand VT2PRO Isoline (DKC 59-89); Pioneer OAM1 = Pioneer Optimum AcreMax1 (P0533AM1); Pioneer non-RW Bt = Pioneer Herculex 1 (P0533HR); Agrisure non-RW Bt = Syngenta Agrisure GT (Agrisure N53-W3, Glyphosate Tolerant); Agrisure 3122 RIB = Syngenta Agrisure RIB (Agrisure N52W-3122 RIB);

Agrisure 3000GT = Syngenta Agrisure Viptera (Agrisure N53W-3000GT) 3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow & T-Band = insecticide applied at planting time; SB = SmartBox application at planting time 5 Chemical and check means based on 16 observations (2-row treatment x 17.5 row-feet/treatment x 4 replications x 2 evaluation dates) 6 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)

18

Table 17. Lodging for Comparison among multiple products: Northeast Iowa Research and Demonstration Farm,

Nashua1


Pioneer OAM1 + SmartChoice-SB 5G 0.25 SB/Furrow 1a Agrisure 3122 RIB + Force 250CS 0.57 T-Band 3a Agrisure 3122 RIB + Counter-SB 20G 0.90 SB/Furrow 3a DeKalb VT3 + Aztec-SB 4.67G 0.14 SB/Furrow 4a DeKalb non-RW Bt + Aztec 2.1G 0.14 Furrow 7a Agrisure 3122 RIB ------- ------- ------- 9ab Pioneer OAM1 ------- ------- ------- 9ab Agrisure 3000GT + Force 250CS 0.57 T-Band 13abc Agrisure 3000GT + Capture LFR 1.5SC 0.10 Furrow 17abcd DeKalb VT3 ------- ------- ------- 30abcd Agrisure non-RW Bt ------- ------- ------- 44 bcd Pioneer non-RW Bt ------- ------- ------- 51 cd Agrisure 3000GT ------- ------- ------- 52 cd DeKalb non-RW Bt ------- ------- ------- 58 d

1 Planted May 7, 2014; evaluated October 9, 2014 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb VT3 = YieldGard VT Triple (DKC58-83); DeKalb-non-RW Bt = DeKalb brand VT2PRO Isoline (DKC 59-89); Pioneer OAM1 = Pioneer Optimum AcreMax1 (P0533AM1); Pioneer non-RW Bt = Pioneer Herculex 1 (P0533HR); Agrisure non-RW Bt = Syngenta Agrisure GT (Agrisure N53-W3, Glyphosate Tolerant); Agrisure 3122 RIB = Syngenta Agrisure RIB (Agrisure N52W-3122 RIB);

Agrisure 3000GT = Syngenta Agrisure Viptera (Agrisure N53W-3000GT) 3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow & T-Band = insecticide applied at planting time; SB = SmartBox application at planting time 5 Chemical and check means based on 8 observations (2-row treatment x 17.5 row-feet/treatment x 4 replications) 6 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)

19

Table 18. Yield for Comparison among Multiple Products: Northeast Iowa Research and Demonstration Farm,

Nashua1

Bushels/


DeKalb non-RW Bt + Aztec 2.1G 0.14 Furrow 193a Agrisure 3000GT + Force 250CS 0.57 T-Band 191a Pioneer OAM1 + SmartChoice-SB 5G 0.25 SB/Furrow 188ab Agrisure 3000GT + Capture LFR 1.5SC 0.10 Furrow 180abc Agrisure 3122 RIB ------- ------- ------- 176abc Agrisure 3122 RIB + Counter-SB 20G 0.90 SB/Furrow 176abc Agrisure 3122 RIB + Force 250CS 0.57 T-Band 176abc DeKalb VT3 + Aztec-SB 4.67G 0.14 SB/Furrow 173abc Pioneer OAM1 ------- ------- ------- 172abcd DeKalb non-RW Bt ------- ------- ------- 153 bcde DeKalb VT3 ------- ------- ------- 153 bcde Agrisure 3000GT ------- ------- ------- 147 cde Pioneer non-RW Bt ------- ------- ------- 140 de Agrisure non-RW Bt ------- ------- ------- 139 e

1 Planted May 7, 2014; machine harvested October 21, 2014 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb VT3 = YieldGard VT Triple (DKC58-83); DeKalb-non-RW Bt = DeKalb brand VT2PRO Isoline (DKC 59-89); Pioneer OAM1 = Pioneer Optimum AcreMax1 (P0533AM1); Pioneer non-RW Bt = Pioneer Herculex 1 (P0533HR); Agrisure non-RW Bt = Syngenta Agrisure GT (Agrisure N53-W3, Glyphosate Tolerant); Agrisure 3122 RIB = Syngenta Agrisure RIB (Agrisure N52W-3122 RIB);

Agrisure 3000GT = Syngenta Agrisure Viptera (Agrisure N53W-3000GT) 3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow & T-Band = insecticide applied at planting time; SB = SmartBox application at planting time 5 Chemical and check means based on 4 observations (2-row treatment x 68 row-feet/treatment x 4 replications) 6 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 7 Yields converted to15.5% Moisture

20

Table 19. Average root injury and product consistency for Monsanto Traits and Insecticides: Northeast Iowa Research and Demonstration Farm, Nashua1

Node- Product


Agrisure 3122 ------ ------ ------ 0.03a 100a Mycogen HXX + Aztec 2.1G 0.14 T-band 0.03a 95a Agrisure 3122 + Aztec 2.1G 0.14 T-band 0.04a 95a DeKalb Smartstax RIB + Aztec 2.1G 0.14 T-band 0.05a 90a DeKalb non-RW Bt + Aztec 2.1G 0.14 T-band 0.12a 80ab DeKalb VT3 + Aztec 2.1G 0.14 T-band 0.13a 95a Mycogen HXX ------ ------ ------ 0.14a 75ab DeKalb Smartstax RIB ------ ------ ------ 0.20a 74ab DeKalb non-RW Bt ------ ------ ------ 0.88 b 35ab DeKalb VT3 ------ ------ ------ 0.92 b 30ab Mycogen non-RW Bt ------ ------ ------ 1.10 b 40ab Agrisure non-RW Bt ------ ------ ------ 1.25 b 20 b

1 Planted May 7, 2014; evaluated July 28, 2014

2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb Smartstax RIB= DeKalb Smartstax RIB (DKC 52-30); DeKalb VT3 = DeKalb VT3 (DKC 52-59); DeKalb non-RW Bt = DeKalb brand RR Isoline (DKC 52-62); Mycogen HXX = Mycogen Herculex XTRA (Mycogen 2K592); Mycogen non-RW Bt = Mycogen brand RR Isoline (Mycogen 2K591); Agrisure 3122 = Syngenta Agrisure (Agrisure N53W-3122); Agrisure non-RW Bt = Syngenta Agrisure (Agrisure N53-W3 GT, Glyphosate Tolerant).

3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 T-band = insecticide applied at planting time 5 Chemical and check means based on 20 observations (5 roots/2 rows x 4 replications) 6 Iowa State Node-Injury Scale (0-3). Number of full or partial nodes completely eaten 7 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 8 Product consistency = Percentage of times nodal injury was 0.25 (¼ node eaten) or less 9 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)

Table 20. Average stand counts for Monsanto Traits and Insecticides: Northeast Iowa Research and Demonstration

Farm, Nashua1

Stand

Treatment2 Form. Form.

Rate3 Placement4 Counts5,6

DeKalb Smartstax RIB + Aztec 2.1G 0.14 T-band 30.00 Mycogen HXX + Aztec 2.1G 0.14 T-band 30.00 Mycogen non-RW Bt ------ ------ ------ 29.50 DeKalb Smartstax RIB ------ ------ ------ 29.50 DeKalb non-RW Bt + Aztec 2.1G 0.14 T-band 29.50 Mycogen HXX ------ ------ ------ 29.50 Agrisure 3122 ------ ------ ------ 29.00 DeKalb non-RW Bt ------ ------ ------ 28.75 DeKalb VT3 + Aztec 2.1G 0.14 T-band 28.50 Agrisure 3122 + Aztec 2.1G 0.14 T-band 28.50 Agrisure non-RW Bt ------ ------ ------ 28.25 DeKalb VT3 ------ ------ ------ 27.25

1 Planted May 7, 2014; evaluated June 6 and October 9, 2014 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb Smartstax RIB= DeKalb Smartstax RIB (DKC 52-30); DeKalb VT3 = DeKalb VT3 (DKC 52-59); DeKalb non-RW Bt = DeKalb brand RR Isoline (DKC 52-62); Mycogen HXX = Mycogen Herculex XTRA (Mycogen 2K592); Mycogen non-RW Bt = Mycogen brand RR Isoline (Mycogen 2K591); Agrisure 3122 = Syngenta Agrisure (Agrisure N53W-3122); Agrisure non-RW Bt = Syngenta Agrisure (Agrisure N53-W3 GT, Glyphosate Tolerant).

3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 T-band = insecticide applied at planting time 5 Chemical and check means based on 16 observations (2 rows/ treatment x 17.5 row-feet/treatment x 4 replications x 2 evaluation dates) 6 No significant differences between means (ANOVA, P < 0.05)

21

Table 21. Average lodging for Monsanto Traits and Insecticides: Northeast Iowa Research and Demonstration Farm, Nashua1


DeKalb Smartstax RIB + Aztec 2.1G 0.14 T-band 1a Mycogen HXX + Aztec 2.1G 0.14 T-band 1a Agrisure 3122 + Aztec 2.1G 0.14 T-band 1a Agrisure 3122 ------ ------ ------ 3a DeKalb VT3 + Aztec 2.1G 0.14 T-band 3a DeKalb Smartstax RIB ------ ------ ------ 4ab Mycogen HXX ------ ------ ------ 4ab DeKalb non-RW Bt + Aztec 2.1G 0.14 T-band 5ab DeKalb VT3 ------ ------ ------ 11ab DeKalb non-RW Bt ------ ------ ------ 18ab Mycogen non-RW Bt ------ ------ ------ 27ab Agrisure non-RW Bt ------ ------ ------ 40 b

1 Planted May 7, 2014; evaluated October 9, 2014 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb Smartstax RIB= DeKalb Smartstax RIB (DKC 52-30); DeKalb VT3 = DeKalb VT3 (DKC 52-59); DeKalb non-RW Bt = DeKalb brand RR Isoline (DKC 52-62); Mycogen HXX = Mycogen Herculex XTRA (Mycogen 2K592); Mycogen non-RW Bt = Mycogen brand RR Isoline (Mycogen 2K591); Agrisure 3122 = Syngenta Agrisure (Agrisure N53W-3122); Agrisure non-RW Bt = Syngenta Agrisure (Agrisure N53-W3 GT, Glyphosate Tolerant).

3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 T-band = insecticide applied at planting time 5 Chemical and check means based on 8 observations (2 rows/ treatment x 17.5 row-feet/treatment x 4 replications) 6 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) Table 22. Average yield for Monsanto Traits and Insecticides: Northeast Iowa Research and Demonstration Farm,

Nashua1

Bushels/


Agrisure 3122 + Aztec 2.1G 0.14 T-band 236 DeKalb Smartstax RIB + Aztec 2.1G 0.14 T-band 230 Agrisure 3122 ------ ------ ------ 221 Mycogen HXX + Aztec 2.1G 0.14 T-band 208 Agrisure non-RW Bt ------ ------ ------ 204 DeKalb non-RW Bt + Aztec 2.1G 0.14 T-band 204 Mycogen HXX ------ ------ ------ 200 DeKalb VT3 + Aztec 2.1G 0.14 T-band 199 DeKalb Smartstax RIB ------ ------ ------ 196 Mycogen non-RW Bt ------ ------ ------ 195 DeKalb VT3 ------ ------ ------ 193 DeKalb non-RW Bt ------ ------ ------ 190

1 Planted May 7, 2014; machine harvested October 21, 2014 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb Smartstax RIB= DeKalb Smartstax RIB (DKC 52-30); DeKalb VT3 = DeKalb VT3 (DKC 52-59); DeKalb non-RW Bt = DeKalb brand RR Isoline (DKC 52-62); Mycogen HXX = Mycogen Herculex XTRA (Mycogen 2K592); Mycogen non-RW Bt = Mycogen brand RR Isoline (Mycogen 2K591); Agrisure 3122 = Syngenta Agrisure (Agrisure N53W-3122); Agrisure non-RW Bt = Syngenta Agrisure (Agrisure N53-W3 GT, Glyphosate Tolerant).

3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 T-band = insecticide applied at planting time 5 Chemical and check means based on 4 observations (2-row treatment x 19 row-feet/treatment x 4 replications) 6 No significant differences between means (ANOVA, P < 0.05) 7 Yields converted to 15.5% Moisture

22

Table 23. Average root-injury and product consistency for Comparison among Bt Rootworm Traits: North Central

Iowa (1) 1

Node- Product


DeKalb Smartstax + Aztec 2.1G 0.14 Furrow 0.03a 100a Mycogen-HXX + Aztec 2.1G 0.14 Furrow 0.04a 100a DeKalb Smartstax ------ ------ ------ 0.05a 97ab DeKalb VT3 + Aztec 2.1G 0.14 Furrow 0.11ab 97ab DeKalb non-RW Bt + Aztec 2.1G 0.14 Furrow 0.14ab 83ab Mycogen-HXX ------ ------ ------ 0.15ab 90ab Mycogen non-RW Bt + Aztec 2.1G 0.14 Furrow 0.30 bc 77ab DeKalb VT3 ------ ------ ------ 0.57 c 53 b Mycogen non-RW Bt ------ ------ ------ 1.53 d 20 c DeKalb non-RW Bt ------ ------ ------ 1.63 d 17 c


2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-RW Bt = DeKalb brand RR (DKC 59-89); DeKalb VT3 = DeKalb brand YieldGard VT Triple (DKC58-83); DeKalb Smartstax = DeKalb brand Smartstax (DKC58-87);

Mycogen non-RW Bt = Mycogen brand RR (Mycogen 2K591); Mycogen-HXX = Mycogen brand Herculex XTRA (Mycogen 2K592)

3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow = insecticide applied at planting time 5 Chemical and check means based on 20 observations (5 roots/2 rows x 4 replications) 6 Iowa State Node-Injury Scale (0-3). Number of full or partial nodes completely eaten 7 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 8 Product consistency = Percentage of times nodal injury was 0.25 (¼ node eaten) or less Table 24. Average stand count for Comparison among Bt Rootworm Traits: North Central Iowa (1) 1

Stand


DeKalb VT3 ------ ------ ------ 34.75a DeKalb VT3 + Aztec 2.1G 0.14 Furrow 34.25ab DeKalb non-RW Bt + Aztec 2.1G 0.14 Furrow 33.25abc Mycogen-HXX + Aztec 2.1G 0.14 Furrow 33.25abc Mycogen non-RW Bt + Aztec 2.1G 0.14 Furrow 32.25abc DeKalb non-RW Bt ------ ------ ------ 33.00abc Mycogen-HXX ------ ------ ------ 32.00abc DeKalb Smartstax ------ ------ ------ 32.00abc DeKalb Smartstax + Aztec 2.1G 0.14 Furrow 31.50 bc Mycogen non-RW Bt ------ ------ ------ 31.50 bc

1 Planted May 22, 2014; evaluated June 10 and October 7, 2014



3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow = Insecticide applied at planting time

5 Chemical and check means based on 16 observations (2 rows/ treatment x 17.5 row-ft/treatment x 4 replications x 2 evaluation dates) 6 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)

23

Table 25. Average lodging for Comparison among Bt Rootworm Traits: North Central Iowa (1) 1


DeKalb non-RW Bt + Aztec 2.1G 0.14 Furrow 1a Mycogen non-RW Bt + Aztec 2.1G 0.14 Furrow 1a Mycogen-HXX + Aztec 2.1G 0.14 Furrow 1a DeKalb VT3 + Aztec 2.1G 0.14 Furrow 2ab Mycogen-HXX ------ ------ ------ 4ab DeKalb VT3 ------ ------ ------ 5abc DeKalb Smartstax + Aztec 2.1G 0.14 Furrow 13abc DeKalb Smartstax ------ ------ ------ 16abc DeKalb non-RW Bt ------ ------ ------ 19 bc Mycogen non-RW Bt ------ ------ ------ 23 c

1 Planted May 22, 2014; evaluated October 7, 2014




5 Chemical and check means based on 8 observations (2 rows/ treatment x 17.5 row-ft/treatment x 4 replications) 6 No significant differences between means (ANOVA, P < 0.05)

Table 26. Average yield for Comparison among Bt Rootworm Traits: North Central Iowa (1) 1

Bushels/


DeKalb VT3 ------ ------ ------ 214a DeKalb Smartstax + Aztec 2.1G 0.14 Furrow 212a DeKalb non-RW Bt + Aztec 2.1G 0.14 Furrow 206ab DeKalb Smartstax ------ ------ ------ 202ab Mycogen-HXX + Aztec 2.1G 0.14 Furrow 195ab DeKalb VT3 + Aztec 2.1G 0.14 Furrow 194ab Mycogen-HXX ------ ------ ------ 188abc DeKalb non-RW Bt ------ ------ ------ 184 bc Mycogen non-RW Bt + Aztec 2.1G 0.14 Furrow 182 bc Mycogen non-RW Bt ------ ------ ------ 171 c

1 Planted May 22, 2014; machine harvested October 31, 2014 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-RW Bt = DeKalb brand RR (DKC 59-89); DeKalb VT3 = DeKalb brand YieldGard VT Triple (DKC58-83); DeKalb Smartstax = DeKalb brand Smartstax (DKC58-87);


3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow = insecticide applied at planting time 5 Chemical and check means based on 4 observations (4-row treatment x 19 row-feet/treatment x 4 replications) 6 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 7 Yields converted to15.5% Moisture

24

Table 27. Average root-injury and product consistency for Syngenta Comparison among Tefluthrin Formulations: North Central Iowa (1) 1

Node- Product


Agrisure non-RW Bt + Force 250CS 0.58 Furrow 0.15a 78a Agrisure non-RW Bt + Force 250CS 0.46 Furrow 0.19a 68a Agrisure non-RW Bt + Aztec 2.1G 0.14 Furrow 0.22a 65a Agrisure non-RW Bt + Capture LFR 1.5SC 0.10 Furrow 0.24a 46a Agrisure non-RW Bt + Force 3G 0.12 Furrow 0.31a 55a Agrisure non-RW Bt + Force 3G 0.14 Furrow 0.33a 50a Agrisure non-RW Bt ------ ------ ------ 1.50 b 13 b


2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; Agrisure non-RW Bt = Syngenta (Golden Harvest brand) glyphosate tolerant hybrid (Agrisure G07V88-GT)

3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow = insecticide applied at planting time 5 Chemical and check means based on 40 observations (10 roots/2 rows x 4 replications) 6 Iowa State Node-Injury Scale (0-3). Number of full or partial nodes completely eaten 7 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 8 Product consistency = Percentage of times nodal injury was 0.25 (¼ node eaten) or less Table 28. Average stand count for Syngenta Comparison among Tefluthrin Formulations: North Central Iowa (1) 1

Stand


Agrisure non-RW Bt ------ ------ ------ 36.00 Agrisure non-RW Bt + Force 250CS 0.58 Furrow 35.00 Agrisure non-RW Bt + Force 3G 0.12 Furrow 34.75 Agrisure non-RW Bt + Force 3G 0.14 Furrow 34.50 Agrisure non-RW Bt + Capture LFR 1.5SC 0.10 Furrow 34.25 Agrisure non-RW Bt + Aztec 2.1G 0.14 Furrow 34.25 Agrisure non-RW Bt + Force 250CS 0.46 Furrow 33.75

1 Planted May 22, 2014; evaluated June 10, 2014

2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; Agrisure non-RW Bt = Syngenta (Golden Harvest brand) glyphosate tolerant hybrid (Agrisure G07V88-GT)


5 Chemical and check means based on 8 observations (2 rows/ treatment x 17.5 row-ft/treatment x 4 replications) 6 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)

25

Table 29. Average yield for Syngenta Comparison among Tefluthrin Formulations: North Central Iowa (1) 1

Bushels/


Agrisure non-RW Bt + Force 250CS 0.46 Furrow 199 Agrisure non-RW Bt ------ ------ ------ 197 Agrisure non-RW Bt + Force 3G 0.14 Furrow 194 Agrisure non-RW Bt + Capture LFR 1.5SC 0.10 Furrow 191 Agrisure non-RW Bt + Aztec 2.1G 0.14 Furrow 191 Agrisure non-RW Bt + Force 250CS 0.58 Furrow 190 Agrisure non-RW Bt + Force 3G 0.12 Furrow 188

1 Planted May 22, 2014; machine harvested October 30, 2014 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; Agrisure non-RW Bt = Syngenta (Golden Harvest brand) glyphosate tolerant hybrid (Agrisure G07V88-GT)

3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow = insecticide applied at planting time 5 Chemical and check means based on 4 observations (4-row treatment x 19 row-feet/treatment x 4 replications) 6 No significant differences between means (ANOVA, P < 0.05) 7 Yields converted to15.5% Moisture Table 30. Average root injury and product consistency for the Dow AgroSciences Smartstax Study: North Central Iowa (1) 1

Node- Product





5 Means based on 20 observations (5 roots/2 rows x 4 replications) 6 Iowa State Node-Injury Scale (0-3). Number of full or partial nodes completely eaten 7 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 8 Product consistency = Percentage of times nodal injury was 0.25 (¼ node eaten) or less 9 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)

26

Table 31. Stand counts for the Dow AgroSciences Smartstax Study: North Central Iowa (1) 1

Stand


Mycogen Smartstax 30.00 Mycogen non-RW Bt 30.00

1 Planted May 22, 2014; evaluated June 26 and October 24, 2014 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; Mycogen non-RW Bt = Mycogen brand RR (Mycogen 2K591); Mycogen Smartstax = Mycogen brand Smartstax (Mycogen 2K594)

3 Means based on 16 observations (2 rows/ treatment x 17.5 row-ft/treatment x 4 replications x 2 evaluation dates) 4 No significant differences between means (ANOVA, P < 0.05) Table 32. Average lodging for the Dow AgroSciences Smartstax Study: North Central Iowa (1) 1

Treatment2 . Lodging3,4

Mycogen Smartstax 10a Mycogen non-RW Bt 45 b

1 Planted May 22, 2014; evaluated October 9, 2014 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; Mycogen non-RW Bt = Mycogen brand RR (Mycogen 2K591); Mycogen Smartstax = Mycogen brand Smartstax (Mycogen 2K594)

3 Means based on 8 observations (2 rows/ treatment x 17.5 row-ft/treatment x 4 replications) 4 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)

Table 33. Average yield for the Dow AgroSciences Smartstax Study: North Central Iowa (1) 1

Bushels/


Mycogen Smartstax 182a Mycogen non-RW Bt 152 b


3 Means based on 4 observations (2 rows/ treatment x 19 row-feet/treatment x 4 replications) 4 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 5 Yields converted to 15.5% Moisture

27

Table 34. Average root-injury and product consistency for Comparison among Bt Rootworm Traits: North Central Iowa (2) 1

Node- Product


DeKalb Smartstax + Aztec 2.1G 0.14 Furrow 0.04a 100a DeKalb Smartstax ------ ------ ------ 0.05a 100a Mycogen-HXX + Aztec 2.1G 0.14 Furrow 0.06a 97a Mycogen-HXX ------ ------ ------ 0.19ab 77a DeKalb VT3 + Aztec 2.1G 0.14 Furrow 0.29 bc 62ab DeKalb non-RW Bt + Aztec 2.1G 0.14 Furrow 0.39 cd 50ab Mycogen non-RW Bt + Aztec 2.1G 0.14 Furrow 0.44 cd 30 bc DeKalb VT3 ------ ------ ------ 0.59 de 37 bc DeKalb non-RW Bt ------ ------ ------ 0.80 ef 13 c Mycogen non-RW Bt ------ ------ ------ 0.88 f 7 c





Stand


Mycogen-HXX ------ ------ ------ 33.75 Mycogen-HXX + Aztec 2.1G 0.14 Furrow 33.50 DeKalb VT3 + Aztec 2.1G 0.14 Furrow 33.25 DeKalb Smartstax + Aztec 2.1G 0.14 Furrow 33.25 DeKalb VT3 ------ ------ ------ 33.00 DeKalb Smartstax ------ ------ ------ 33.00 Mycogen non-RW Bt ------ ------ ------ 32.50 Mycogen non-RW Bt + Aztec 2.1G 0.14 Furrow 32.25 DeKalb non-RW Bt ------ ------ ------ 32.25 DeKalb non-RW Bt + Aztec 2.1G 0.14 Furrow 31.75





5 Chemical and check means based on 16 observations (2 rows/ treatment x 17.5 row-ft/treatment x 4 replications x 2 evaluation dates) 6 No significant differences between means (ANOVA, P < 0.05)

28

Table 36. Average lodging for Comparison among Bt Rootworm Traits: North Central Iowa (2) 1


DeKalb VT3 + Aztec 2.1G 0.14 Furrow 0 DeKalb Smartstax ------ ------ ------ 0 DeKalb Smartstax + Aztec 2.1G 0.14 Furrow 0 Mycogen non-RW Bt ------ ------ ------ 0 Mycogen non-RW Bt + Aztec 2.1G 0.14 Furrow 0 Mycogen-HXX ------ ------ ------ 0 Mycogen-HXX + Aztec 2.1G 0.14 Furrow 0 DeKalb non-RW Bt + Aztec 2.1G 0.14 Furrow 1 DeKalb non-RW Bt ------ ------ ------ 4 DeKalb VT3 ------ ------ ------ 5







Bushels/


DeKalb Smartstax + Aztec 2.1G 0.14 Furrow 200a DeKalb Smartstax ------ ------ ------ 197ab Mycogen-HXX + Aztec 2.1G 0.14 Furrow 191ab DeKalb non-RW Bt ------ ------ ------ 188ab DeKalb non-RW Bt + Aztec 2.1G 0.14 Furrow 187ab Mycogen-HXX ------ ------ ------ 187ab Mycogen non-RW Bt ------ ------ ------ 182ab Mycogen non-RW Bt + Aztec 2.1G 0.14 Furrow 180ab DeKalb VT3 ------ ------ ------ 173ab DeKalb VT3 + Aztec 2.1G 0.14 Furrow 169 b



3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow = insecticide applied at planting time 5 Chemical and check means based on 4 observations (4-row treatment x 19 row-feet/treatment x 4 replications) 6 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 7 Yields converted to15.5% Moisture

29

Table 38. Average root-injury and product consistency for Comparison among Bt Rootworm Traits: North Central

Iowa (3) 1

Node- Product


DeKalb VT3 + Aztec 2.1G 0.14 Furrow 0.00a 100a DeKalb non-RW Bt ------ ------ ------ 0.01ab 100a DeKalb non-RW Bt + Aztec 2.1G 0.14 Furrow 0.01ab 100a DeKalb VT3 ------ ------ ------ 0.01ab 100a DeKalb Smartstax ------ ------ ------ 0.01ab 100a DeKalb Smartstax + Aztec 2.1G 0.14 Furrow 0.01ab 100a Mycogen non-RW Bt + Aztec 2.1G 0.14 Furrow 0.01ab 100a Mycogen-HXX ------ ------ ------ 0.01ab 100a Mycogen-HXX + Aztec 2.1G 0.14 Furrow 0.01ab 100a Mycogen non-RW Bt ------ ------ ------ 0.03 b 93 b





Stand


DeKalb VT3 ------ ------ ------ 33.75 Mycogen-HXX + Aztec 2.1G 0.14 Furrow 33.25 DeKalb Smartstax ------ ------ ------ 33.25 DeKalb non-RW Bt ------ ------ ------ 33.00 DeKalb VT3 + Aztec 2.1G 0.14 Furrow 33.00 Mycogen-HXX ------ ------ ------ 32.75 DeKalb non-RW Bt + Aztec 2.1G 0.14 Furrow 32.75 DeKalb Smartstax + Aztec 2.1G 0.14 Furrow 32.50 Mycogen non-RW Bt + Aztec 2.1G 0.14 Furrow 32.50 Mycogen non-RW Bt ------ ------ ------ 32.25






30


Bushels/


DeKalb non-RW Bt ------ ------ ------ 166 DeKalb Smartstax ------ ------ ------ 159 DeKalb non-RW Bt + Aztec 2.1G 0.14 Furrow 152 DeKalb VT3 ------ ------ ------ 152 Mycogen non-RW Bt + Aztec 2.1G 0.14 Furrow 149 DeKalb Smartstax + Aztec 2.1G 0.14 Furrow 149 Mycogen-HXX ------ ------ ------ 147 Mycogen-HXX + Aztec 2.1G 0.14 Furrow 145 Mycogen non-RW Bt ------ ------ ------ 142 DeKalb VT3 + Aztec 2.1G 0.14 Furrow 125



3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow = insecticide applied at planting time 5 Chemical and check means based on 4 observations (4-row treatment x 19 row-feet/treatment x 4 replications) 6 No significant differences between means (ANOVA, P < 0.05) 7 Yields converted to15.5% Moisture

31

Table 41. Average root injury and product consistency for the Dow AgroSciences Smartstax Study: North Central Iowa (3) 1

Node- Product


Mycogen non-RW Bt 0.02 100 Mycogen Smartstax 0.03 95



3 Means based on 20 observations (5 roots/2 rows x 4 replications)

4 Iowa State Node-Injury Scale (0-3). Number of full or partial nodes completely eaten 5 No significant differences between means (ANOVA, P < 0.05) 6 Product consistency = Percentage of times nodal injury was 0.25 (¼ node eaten) or less 7 No significant differences between means (ANOVA, P < 0.05) Table 42. Stand counts for the Dow AgroSciences Smartstax Study: North Central Iowa (3) 1

Stand




3 Means based on 16 observations (2 rows/ treatment x 17.5 row-ft/treatment x 4 replications x 2 evaluation dates) 4 No significant differences between means (ANOVA, P < 0.05) Table 43. Average yield for the Dow AgroSciences Smartstax Study: North Central Iowa (3) 1

Bushels/





32

Table 44. Average root injury and product consistency for Monsanto Traits and Insecticides: North Central Iowa (3) 1

Node- Product


Agrisure 3122 ------ ------ ------ 0.01ab 100 DeKalb non-RW Bt + Aztec 2.1G 0.14 T-band 0.01ab 100 Agrisure 3122 + Aztec 2.1G 0.14 T-band 0.01ab 100 Agrisure non-RW Bt ------ ------ ------ 0.02ab 100 DeKalb Smartstax RIB ------ ------ ------ 0.02a 100 DeKalb VT3 ------ ------ ------ 0.02ab 100 DeKalb non-RW Bt ------ ------ ------ 0.02ab 100 DeKalb Smartstax RIB + Aztec 2.1G 0.14 T-band 0.03ab 100 DeKalb VT3 + Aztec 2.1G 0.14 T-band 0.03ab 100 Mycogen HXX ------ ------ ------ 0.03ab 100 Mycogen non-RW Bt ------ ------ ------ 0.03ab 100 Mycogen HXX + Aztec 2.1G 0.14 T-band 0.04 b 100


2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb Smartstax RIB= DeKalb Smartstax RIB (DKC 52-30); DeKalb VT3 = DeKalb VT3 (DKC 52-59); DeKalb non-RW Bt = DeKalb brand RR Isoline (DKC 52-62); Mycogen HXX = Mycogen Herculex XTRA (Mycogen 2K592); Mycogen non-RW Bt = Mycogen brand RR Isoline (Mycogen 2K591); Agrisure 3122 = Syngenta Agrisure (Agrisure N53W-3122); Agrisure non-RW Bt = Syngenta Agrisure (Agrisure N53-W3 GT, Glyphosate Tolerant).

3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 T-band = insecticide applied at planting time 5 Chemical and check means based on 20 observations (5 roots/2 rows x 4 replications) 6 Iowa State Node-Injury Scale (0-3). Number of full or partial nodes completely eaten 7 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 8 Product consistency = Percentage of times nodal injury was 0.25 (¼ node eaten) or less 9 No significant differences between means (ANOVA, P < 0.05) Table 45. Average stand counts for Monsanto Traits and Insecticides: North Central Iowa (3) 1

Stand

Treatment2 Form. Form.

Rate3 Placement4 Counts5,6

Mycogen HXX ------ ------ ------ 34.00a DeKalb non-RW Bt + Aztec 2.1G 0.14 T-band 33.50ab Mycogen HXX + Aztec 2.1G 0.14 T-band 33.50ab DeKalb Smartstax RIB ------ ------ ------ 33.00ab DeKalb Smartstax RIB + Aztec 2.1G 0.14 T-band 32.75abc DeKalb non-RW Bt ------ ------ ------ 32.50abc Agrisure non-RW Bt ------ ------ ------ 32.00abc Mycogen non-RW Bt ------ ------ ------ 31.75abc Agrisure 3122 ------ ------ ------ 31.75abc Agrisure 3122 + Aztec 2.1G 0.14 T-band 31.75abc DeKalb VT3 ------ ------ ------ 31.00 bc DeKalb VT3 + Aztec 2.1G 0.14 T-band 30.25 c

1 Planted May 22, 2014; evaluated June 10 and October 7, 2014 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb Smartstax RIB= DeKalb Smartstax RIB (DKC 52-30); DeKalb VT3 = DeKalb VT3 (DKC 52-59); DeKalb non-RW Bt = DeKalb brand RR Isoline (DKC 52-62); Mycogen HXX = Mycogen Herculex XTRA (Mycogen 2K592); Mycogen non-RW Bt = Mycogen brand RR Isoline (Mycogen 2K591); Agrisure 3122 = Syngenta Agrisure (Agrisure N53W-3122); Agrisure non-RW Bt = Syngenta Agrisure (Agrisure N53-W3 GT, Glyphosate Tolerant).

3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 T-band = insecticide applied at planting time 5 Chemical and check means based on 16 observations (2 rows/ treatment x 17.5 row-feet/treatment x 4 replications x 2 evaluation dates) 6 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)

33

Table 46. Average yield for Monsanto Traits and Insecticides: North Central Iowa (3) 1

Bushels/


Agrisure 3122 ------ ------ ------ 235 DeKalb non-RW Bt ------ ------ ------ 230 Mycogen HXX ------ ------ ------ 226 DeKalb Smartstax RIB + Aztec 2.1G 0.14 T-band 222 Mycogen HXX + Aztec 2.1G 0.14 T-band 221 DeKalb Smartstax RIB ------ ------ ------ 221 DeKalb VT3 ------ ------ ------ 212 Mycogen non-RW Bt ------ ------ ------ 212 Agrisure 3122 + Aztec 2.1G 0.14 T-band 212 Agrisure non-RW Bt ------ ------ ------ 208 DeKalb non-RW Bt + Aztec 2.1G 0.14 T-band 205 DeKalb VT3 + Aztec 2.1G 0.14 T-band 203

1 Planted May 22, 2014; machine harvested October 30, 2014 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; DeKalb Smartstax RIB= DeKalb Smartstax RIB (DKC 52-30); DeKalb VT3 = DeKalb VT3 (DKC 52-59); DeKalb non-RW Bt = DeKalb brand RR Isoline (DKC 52-62); Mycogen HXX = Mycogen Herculex XTRA (Mycogen 2K592); Mycogen non-RW Bt = Mycogen brand RR Isoline (Mycogen 2K591); Agrisure 3122 = Syngenta Agrisure (Agrisure N53W-3122); Agrisure non-RW Bt = Syngenta Agrisure (Agrisure N53-W3 GT, Glyphosate Tolerant).

3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 T-band = insecticide applied at planting time 5 Chemical and check means based on 4 observations (2-row treatment x 19 row-feet/treatment x 4 replications) 6 No significant differences between means (ANOVA, P < 0.05) 7 Yields converted to 15.5% Moisture Table 47. Average root-injury and product consistency for Comparison among Bt Rootworm Traits: North Eastern

Iowa 1

Node- Product


Mycogen-HXX + Aztec 2.1G 0.14 Furrow 0.04a 100a DeKalb Smartstax ------ ------ ------ 0.04a 100a DeKalb Smartstax + Aztec 2.1G 0.14 Furrow 0.08a 97a DeKalb non-RW Bt + Aztec 2.1G 0.14 Furrow 0.06a 97a Mycogen non-RW Bt + Aztec 2.1G 0.14 Furrow 0.07a 100a DeKalb VT3 + Aztec 2.1G 0.14 Furrow 0.10a 93a DeKalb VT3 ------ ------ ------ 0.11a 87a Mycogen-HXX ------ ------ ------ 0.13a 93a DeKalb non-RW Bt ------ ------ ------ 0.60 b 47 b Mycogen non-RW Bt ------ ------ ------ 0.85 b 40 b




3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow = insecticide applied at planting time 5 Chemical and check means based on 20 observations (5 roots/2 rows x 4 replications) 6 Iowa State Node-Injury Scale (0-3). Number of full or partial nodes completely eaten 7 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 8 Product consistency = Percentage of times nodal injury was 0.25 (¼ node eaten) or less

34

Table 48. Average stand count for Comparison among Bt Rootworm Traits: North Eastern Iowa 1

Stand


DeKalb non-RW Bt + Aztec 2.1G 0.14 Furrow 33.25 Mycogen-HXX ------ ------ ------ 33.25 Mycogen non-RW Bt ------ ------ ------ 33.25 DeKalb Smartstax + Aztec 2.1G 0.14 Furrow 33.00 Mycogen non-RW Bt + Aztec 2.1G 0.14 Furrow 33.00 Mycogen-HXX + Aztec 2.1G 0.14 Furrow 32.75 DeKalb VT3 ------ ------ ------ 32.25 DeKalb Smartstax ------ ------ ------ 32.25 DeKalb VT3 + Aztec 2.1G 0.14 Furrow 31.50 DeKalb non-RW Bt ------ ------ ------ 31.25






Table 49. Average lodging for Comparison among Bt Rootworm Traits: North Eastern Iowa 1


DeKalb non-RW Bt + Aztec 2.1G 0.14 Furrow 0 DeKalb VT3 ------ ------ ------ 0 DeKalb Smartstax ------ ------ ------ 0 Mycogen non-RW Bt + Aztec 2.1G 0.14 Furrow 0 Mycogen-HXX + Aztec 2.1G 0.14 Furrow 0 DeKalb Smartstax + Aztec 2.1G 0.14 Furrow 1 Mycogen non-RW Bt ------ ------ ------ 1 Mycogen-HXX ------ ------ ------ 1 DeKalb VT3 + Aztec 2.1G 0.14 Furrow 1 DeKalb non-RW Bt ------ ------ ------ 2






35

Table 50. Average yield for Comparison among Bt Rootworm Traits: North Eastern Iowa 1

Bushels/


Mycogen-HXX + Aztec 2.1G 0.14 Furrow 167a DeKalb non-RW Bt + Aztec 2.1G 0.14 Furrow 158a DeKalb Smartstax + Aztec 2.1G 0.14 Furrow 155ab Mycogen non-RW Bt + Aztec 2.1G 0.14 Furrow 154ab DeKalb Smartstax ------ ------ ------ 148ab Mycogen non-RW Bt ------ ------ ------ 144ab Mycogen-HXX ------ ------ ------ 139ab DeKalb non-RW Bt ------ ------ ------ 134ab DeKalb VT3 ------ ------ ------ 130ab DeKalb VT3 + Aztec 2.1G 0.14 Furrow 118 b



3 Insecticide listed as ounces a.i. per 1,000 row-feet 4 Furrow = insecticide applied at planting time 5 Chemical and check means based on 4 observations (4-row treatment x 19 row-feet/treatment x 4 replications) 6 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 7 Yields converted to15.5% Moisture Table 51. Average root injury and product consistency for the Dow AgroSciences Smartstax Study: North Eastern Iowa1

Node- Product





3 Means based on 20 observations (5 roots/2 rows x 4 replications) 4 Iowa State Node-Injury Scale (0-3). Number of full or partial nodes completely eaten 5 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05) 6 Product consistency = Percentage of times nodal injury was 0.25 (¼ node eaten) or less 7 Means sharing a common letter do not differ significantly according to Ryan’s Q Test (P < 0.05)

36

Table 52. Stand counts for the Dow AgroSciences Smartstax Study: North Eastern Iowa 1

Stand




3 Means based on 16 observations (2 rows/ treatment x 17.5 row-ft/treatment x 4 replications x 2 evaluation dates) 4 No significant differences between means (ANOVA, P < 0.05) Table 53. Lodging for the Dow AgroSciences Smartstax Study: North Eastern Iowa 1

Treatment2 Lodging3,4


1 Planted May 28, 2014; evaluated October 9, 2014 2 non-RW Bt = an absence of any Bt trait targeting corn rootworm; Mycogen non-RW Bt = Mycogen brand RR (Mycogen 2K591); Mycogen Smartstax = Mycogen brand Smartstax (Mycogen 2K594)

3 Means based on 8 observations (2 rows/ treatment x 17.5 row-ft/treatment x 4 replications) 4 No significant differences between means (ANOVA, P < 0.05) Table 54. Average yield for the Dow AgroSciences Smartstax Study: North Eastern Iowa1

Bushels/





37

APPENDIX I

Agronomic Information

38

2014 Field History Data

Ames, IA-ISU Johnson Farm Boone, IA-ISU Bruner Farm

Corn Rootworm studies Corn Rootworm studies (following trap crop) (following continuous corn)

Insecticide History

2013 No Insecticide (trap crop) Corn (Insecticide Test Plot)

2012 Insecticide Test Plot Corn

2011 No Insecticide (trap crop) Corn 2010 Insecticide Test Plot Corn

Tillage Fall 2013-chopped stalks & disk ripper Spring 2014-field cultivation (2X)

Fall 2013-chopped stalks & disk ripper Spring 2014-field cultivation (2X)

Variety Mycogen 2K591 RR2, Mycogen 2K594 SSTX, DeKalb DKC 58-89 VT2PRO, DeKalb DKC 58-83 VT3, Agrisure N53W-3122 RIB, Agrisure N53-W3 GT, Pioneer P0533AM1, Pioneer P0533HR, DeKalb DKC 62-95 RR2, DeKalb 62-08 SSTX

DeKalb DKC 62-95 RR2, DeKalb 62-08 Smartstax

Planting Date

May 19, 20 & May 30

May 29

Planting Rate 35,600 seeds/A 35,600 seeds/A

Herbicides1 2.5 pts. Harness-May 7 10.18 oz. Synergize-June 6 3 oz. Laudis-June 13

Fertilizer2

N P K S Z

N P K

Fall Applied 12 40 0 10 1 --- --- ---

Preplant 200 --- --- 186 --- ---

Dates

Stand Count June 9 & 10, September 23 & 25 June 10; September 29

Root Digging August 4 & 5 August 19

Lodging September 23, 25 September 29 Harvest

October 28 October 31

Soil Type Clay loam & loam Silty clay loam

Soil Organic Matter % 3.7 3.5 Soil pH 6.2 6.1

1 Expressed as formulation per acre. 2 Expressed as pounds per acre. Spring-preplant 200 lbs., 32% UAN applied on May 7 at ISU Johnson Farm (Ames); Fall applied- 22 lbs. Nitrogen, 74 lbs. Phosphorus, 111 lbs. Potassium, 18.5 lbs. Sulfur, 1.8 lbs. Zinc applied on November 12, 2013 at ISU Johnson Farm (Ames); Spring-preplant 186 lbs., UAN applied on May 20 at ISU Bruner Farm (Boone);

39


1 Expressed as formulation per acre. 2 Expressed as pounds per acre. Spring-Preplant 200 lbs. per acre actual nitrogen applied as anhydrous

ammonia (82.5-0- 0) on April 19 at Nashua (Both fields); Fall applied- spread 360 lbs per acre of 2 ton DAP/ 4

ton of K = 55.2 lbs P205/ac and 144 lbs K20/ac on November 10, 2014 at Nashua (Both fields)

Nashua, IA Nashua, IA Corn Rootworm studies Corn Rootworm studies (following trap crop) (following continuous corn)

Insecticide History 2013 No Insecticide (trap crop) VT3 Hybrid Test Plot (Aztec) 2012 Insecticide Test Plot VT3 Hybrid Test Plot 2011 No Insecticide (trap crop) VT3 Hybrid Test Plot 2010 Insecticide Test Plot YIELDGARD + Hybrid Test Plot Tillage

Fall 2013-chisel plow; Spring 2014-field cultivation; Fall 2014-chisel plow

Fall 2013-chisel plow; Spring 2014-field cultivation; Fall 2014-chisel plow

Variety DeKalb DKC 58-89 VT2PRO,

DeKalb DKC 58-83 VT3, Agrisure N53W-3122 RIB, Agrisure N53W-3000GT, Agrisure N53-W3 GT, Pioneer P0533AM1, Pioneer P0533HR

DeKalb DKC 52-30 Smartstax RIB, DeKalb DKC 52-59 VT3, DeKalb DKC 52-62 RR2, Mycogen 2K591 RR2, Mycogen 2K592 HXX, Agrisure N53W-3122 NOT RIB, Agrisure N53-W3 GT

Planting Date(s)

May 7

May 7


Herbicides1 3.5 pts. G Max Lite-May 8; 6 oz. Clarity-June 3

3.5 pts. G Max Lite-May 8; 6 oz. Clarity-June 3

Fertilizer2

N P K

N P K

Fall Applied (2014) 360 55.2 144 --- --- --- Preplant 200 --- --- 200 --- --- Dates

Stand Count June 6 & October 9 June 6 & October 9 Root Digging July 25 July 24 Lodging October 9 October 9 Harvest


Soil Type Loam Loam Soil Organic Matter % 3.9 3.9 Soil pH 7.6 7.6

40


1 Expressed as formulation per acre. 2 Expressed as pounds per acre. Spring-Preplant 200 lbs. 32% UAN on April 26 at North Central Iowa (1) location; Spring-Preplant 140 lbs. actual nitrogen applied as anhydrous ammonia (82.5-0- 0) on May 13 at

North Central Iowa (2) location

North Central Iowa (1) North Central Iowa (2) Corn Rootworm studies Corn Rootworm studies (following continuous corn) (following continuous corn)

Insecticide History 2013 Corn-VT3 PRO-RIB (No Insecticide) Corn-VT3 RIB (No Insecticide) 2012 Corn-VT3-Block (No Insecticide) Corn-VT3 Block (No Insecticide) 2011 Corn (No Insecticide) Corn-VT3 Block (No Insecticide)

2010 Corn (No Insecticide) Corn (No Insecticide) Tillage Fall 2013-disk ripper;

Spring 2014-field cultivation Fall 2013-disk ripper; Spring 2014-field cultivation

Variety Mycogen 2K591 RR2, Mycogen

2K592 HXX, Mycogen 2K594 SSTX, DeKalb DKC 58-89 VT2PRO, DeKalb DKC 58-83 VT3, DeKalb DKC 58-87 Smartstax, Syngenta Agrisure GO7V88-GT (Golden Harvest brand)

Mycogen 2K591 RR2, Mycogen 2K592 HXX, DeKalb DKC 58-89 VT2PRO, DeKalb DKC 58-83 VT3, DeKalb DKC 58-87 Smartstax

Planting Date(s)

May 22

May 23


Herbicide1 2.5 pts. Volley (acetochlor) + 0.75 lbs. Atrazine (PPI)-April 26 0.75 oz. Impact + 24 oz. Duramax (POST)-April 26

4 oz Balance Flexx + 32% UAN (PRE)-May 20 3 oz Capreno + 0.5 lbs. Atrazine (POST)-June 11

Fertilizer2

N P K

N P K

Fall Applied --- --- --- --- --- --- Preplant

200 --- --- 140 --- ---

Dates Stand Count June 10 and October 7 June 10 and October 7 Root Digging August 13 August 13 Lodging October 7 October 7 Harvest October 30 October 30 Soil Type Clay loam Silty clay loam Soil Organic Matter % 3.29 4.33 Soil pH 6.1 7.0

41


1 Expressed as formulation per acre. 2 Expressed as pounds per acre. Spring-preplant 150 lbs. 32% UAN on May 6 at North Central Iowa (3) location; Fall applied-27-70-70 (27 lbs. N, 70 lbs. P, 70 lbs K) dry fertilizer was applied on November 16, 2013

at North Central Iowa (3) location; Spring-preplant 100 lbs. 32% UAN applied on May 21 with pre-

emergence herbicide at North Eastern Iowa location; Spring/Summer-side dressed 100 lbs. 32% UAN

applied on June 11 at North Eastern Iowa location

North Central Iowa (3) North Eastern Iowa Corn Rootworm studies Corn Rootworm studies (following continuous corn) (following continuous corn)

Insecticide History 2013 Corn-VT3 PRO RIB (No

Insecticide) Corn-VT3 PRO RIB (No Insecticide)

2012 Corn-Smartstax RIB (No Insecticide)

Corn-VT3 PRO RIB (No Insecticide)

2011 Corn-VT3 PRO/Smartstax-Block (No Insecticide)

Corn-VT3 PRO RIB (No Insecticide)

2010 Corn-VT3-Block (No Insecticide) Corn-VT3 PRO RIB (No Insecticide) Tillage Fall 2013-disk ripper;

Spring 2014-field cultivation Fall 2013-disk; Spring 2014-field cultivation

Variety Mycogen 2K591 RR2, Mycogen

2K592 HXX, Mycogen 2K594 Smartstax, DeKalb DKC 58-89 VT2PRO, DeKalb DKC 58-83 VT3, DeKalb DKC 58-87 Smartstax, DeKalb DKC 52-30 Smartstax RIB, DeKalb DKC 52-59 VT3, DeKalb DKC 52-62 RR2, Agrisure N53W-3122 NOT RIB, Agrisure N53-W3 GT

Mycogen 2K591 RR2, Mycogen 2K592 HXX, Mycogen 2K295 Smartstax, DeKalb DKC 58-89 VT2PRO, DeKalb DKC 58-83 VT3, DeKalb DKC 58-87 Smartstax

Planting Date(s)

May 22

May 28


Herbicides1 14 oz. Verdict (PPI)-May 6 2 oz. Callisto + 32 oz. Roundup WeatherMax (POST)-May 6

2 pts. Sure Start-May 21 32 oz. Roundup PowerMax-June 15

Fertilizer2

N P K

N P K

Fall Applied 27 70 70 --- --- --- Preplant 150 --- --- 200 --- --- Dates

Stand Count June 10 and October 7 June 12 and October 7 Root Digging August 13 August 15 Lodging October 7 October 7 Harvest


Soil Type loam loam Soil Organic Matter % 3.88 5.1 Soil pH 6.9 7.0

42

APPENDIX II

Weather Data

43

Ames & Boone1

2014 Rainfall and Temperature

May June July

Temp (F) Rainfall Temp (F) Rainfall Temp (F) Rainfall

Day Low High Inches Low High Inches Low High Inches

1 40 47 0.01 65 89 0.80 57 76 0.04 2 38 66 63 82 57 70 3 42 71 0.01 56 77 0.94 53 77 4 44 62 61 76 0.07 57 79 5 44 69 58 74 62 76 0.85 6 46 75 59 86 68 89 7 54 89 59 72 0.48 67 86 0.01 8 54 81 0.01 57 74 63 80 9 49 69 56 74 57 80 10 47 81 59 74 59 82 11 51 75 0.79 57 83 64 82 12 48 73 0.46 56 74 66 82 1.10 13 45 58 50 77 63 84 0.05 14 40 62 0.15 56 82 55 72 15 38 49 62 82 52 72 16 43 56 60 88 0.55 54 77 17 38 67 62 85 0.69 55 78 18 44 70 67 87 0.02 57 78 19 56 74 0.03 65 79 1.08 62 81 20 55 71 1.67 61 88 62 83 21 55 85 66 85 68 89 22 50 81 65 76 0.98 68 88 23 49 77 63 83 0.01 61 82 24 52 78 63 82 59 77 0.04 25 62 77 0.04 63 86 63 80 0.16 26 63 79 0.72 67 79 1.25 68 85 27 60 77 0.10 67 79 0.75 62 75 28 58 81 63 85 0.28 59 75 29 59 80 61 81 0.01 56 78 30 63 83 64 77 1.29 57 81 31 64 86 57 81

Mean/Total 63.0 3.99 71.0 9.20 68.0 2.25

Normal 59.6 4.81 69.4 5.23 73.2 4.84 D.F.N. -3.4 -0.82 1.6 3.97 -5.2 -2.59

1Weather station located 5 miles WSW of Ames at ISU AEA/Agronomy Farm.

44

Ames & Boone1


August September October



1 57 85 61 78 54 71 0.89 2 63 83 58 78 0.05 50 65 0.26 3 62 85 58 83 37 52 4 63 87 73 92 33 53 5 67 80 53 74 0.70 40 65 6 64 69 1.06 49 76 0.01 42 65 7 67 71 0.06 51 74 47 68 8 63 79 52 70 0.04 48 70 0.06 9 64 79 64 73 1.32 44 63 0.10 10 63 79 49 68 0.08 36 55 11 61 78 47 56 35 60 12 55 77 37 53 0.29 37 56 0.01 13 56 81 35 60 51 53 1.33 14 58 81 46 70 49 63 0.21 15 66 70 0.31 46 61 43 68 16 65 80 40 68 39 75 17 63 85 48 77 47 60 18 66 86 49 72 38 59 19 67 84 53 80 35 67 20 64 77 0.27 56 83 0.31 44 70 21 73 86 0.13 50 68 40 65 22 70 87 0.15 45 75 40 65 0.04 23 71 84 0.34 48 70 47 71 0.51 24 71 90 56 69 0.90 45 72 0.01 25 67 87 57 79 50 70 26 63 80 0.90 58 81 42 68 27 63 76 0.01 59 82 49 67 28 65 78 1.16 58 82 39 54 29 65 83 1.10 55 82 37 54 30 64 80 53 67 35 60 31 64 81 26 61

Mean/Total 75.0 5.49 63.0 4.02 54.0 3.42 Noal 71.1 4.78 63.0 3.28 50.5 2.57 D.F.N. 3.9 0.71 0.0 0.74 3.5 0.85

1Weather station located 5 miles WSW of Ames at ISU AEA/Agronomy Farm.

45

North Central Iowa1


May June July



1 35 40 0.67 65 84 0.04 59 68 1.45 2 35 46 0.01 62 85 0.78 54 74 0.0001 3 34 66 52 79 47 70 4 40 70 0.06 55 75 0.65 50 73 5 41 59 55 75 56 77 0.12 6 44 68 57 74 62 74 7 45 72 60 84 61 88 8 53 85 0.02 52 73 60 83 0.01 9 47 78 0.85 57 75 52 78

10 47 79 54 75 53 78 11 49 76 0.05 51 74 62 83 12 49 72 2.50 53 82 65 76 0.21 13 44 71 0.23 43 73 57 79 14 34 56 51 75 55 82 15 38 62 0.0001 60 76 0.20 49 71 16 30 48 0.14 57 79 47 71 17 30 58 60 83 2.82 49 75 18 31 66 61 82 0.22 51 73 19 45 69 65 84 0.02 56 76 20 54 69 0.02 63 78 0.10 62 77 21 55 86 0.0001 65 84 0.05 63 82 22 47 78 64 84 0.30 67 86 23 49 78 60 75 0.81 56 86 24 49 78 57 81 55 81 25 57 80 58 82 61 79 1.87 26 59 77 0.0001 61 83 0.14 64 78 27 59 79 0.43 64 80 1.01 64 84 28 60 79 0.03 66 79 0.52 56 72 29 59 78 58 82 0.85 51 72 0.0001 30 61 85 62 82 2.07 52 77 31 64 85 0.01 51 79

Mean/Total 58.7 5.02 66.5 10.58 66.9 3.66 Normal 59.7 4.69 69.6 5.36 73.1 4.42 D.F.N. -1.0 0.33 -3.1 5.22 -6.2 -0.76

1Weather station located approximately 3 miles from studies location

46

North Central Iowa1





1 52 81 59 85 1.20 52 69 1.04 2 55 80 57 77 0.26 53 69 0.11 3 60 80 55 77 43 63 0.21 4 62 83 58 82 32 45 5 63 84 57 88 0.43 32 52 6 63 81 0.15 44 71 0.14 37 61 7 64 67 0.66 46 74 40 69 8 63 73 0.0001 48 75 34 66 9 62 77 0.0001 52 72 0.22 37 66 0.0001

10 62 77 63 71 0.63 32 70 11 62 77 45 65 0.0001 27 56 12 51 77 42 57 0.23 30 67 13 49 76 31 54 0.19 34 57 0.0001 14 50 81 34 59 48 53 1.22 15 55 79 0.0001 42 70 37 62 16 65 68 0.31 34 59 34 67 17 62 79 34 66 34 75 18 61 84 43 75 38 57 19 63 85 43 72 39 55 20 59 84 0.0001 52 79 0.49 36 67 21 65 77 51 80 0.0001 34 70 22 72 88 41 67 35 66 23 71 88 0.20 42 72 41 62 0.42 24 70 82 1.40 48 71 0.57 43 69 25 67 88 0.02 53 67 0.21 47 72 26 62 82 0.20 58 79 38 67 27 61 76 54 80 39 68 28 61 74 0.19 54 77 40 67 29 64 74 0.79 57 82 33 53 30 64 79 0.45 50 73 33 54 31 57 78 24 60

Mean/Total 70.3 4.37 58.4 4.57 50.2 3.00 Normal 70.8 3.82 62.5 3.17 50.1 2.26 D.F.N. -0.5 0.55 -4.1 1.40 0.1 0.74

1Weather station located approximately 3 miles from studies location

47

Nashua1 & North Eastern Iowa3 location


May June July



1 37 49 0.04 66 90 0.45 58 77 0.10 2 40 66 60 83 0.05 53 70 T 3 38 66 54 78 0.01 48 77 4 42 57 0.13 61 77 0.01 49 79 5 47 67 53 81 59 74 6 45 70 55 88 64 88 7 49 84 61 75 0.09 59 84 8 60 80 0.40 52 75 61 79 9 48 64 55 76 52 81

10 39 70 0.05 57 77 57 84 11 49 72 0.17 52 86 62 78 0.14 12 57 74 1.69 57 73 65 80 0.69 13 43 55 46 80 58 81 14 35 61 54 81 0.10 55 71 15 41 48 0.24 67 85 51 71 16 33 53 T 57 86 46 77 17 33 67 61 85 3.66 49 79 18 39 73 62 84 0.58 55 79 19 56 87 0.03 65 72 1.38 62 81 20 56 78 0.01 63 89 64 84 21 54 78 67 88 67 89 22 46 77 63 79 0.53 73 91 T 23 46 79 61 82 T 58 83 24 51 79 59 84 54 82 25 53 78 60 85 61 82 0.44 26 61 84 0.06 61 85 0.02 63 84 T 27 58 87 T 65 78 0.92 61 75 0.04 28 60 80 T 67 85 0.78 64 77 29 55 87 59 81 0.862 51 78 30 61 91 63 81 0.91 51 81 31 66 89 0.05 53 83

Mean/Total 60.2 2.87 70.5 10.35 68.6 1.41 Normal 59.4 4.44 68.9 5.11 72.0 4.69

D.F.N. 0.8 -1.57 1.6 5.24 -3.4 -3.28

1Weather station located at test site. 2 Hail was reported at this field site on June 29 3 Weather station located approximately 5 miles northwest of field location

48

Nashua1 & North Eastern Iowa2 locations1




Day High Low Inches High Low Inches High Low Inches

1 53 84 60 86 1.31 49 70 0.15 2 56 86 55 79 0.04 56 68 0.54 3 60 85 0.20 53 84 41 52 4 62 85 0.63 68 89 33 53 0.02 5 59 79 58 69 0.10 30 54 6 60 77 45 77 34 62 7 59 77 47 77 44 65 0.03 8 63 82 50 73 34 69 9 58 84 61 75 0.19 36 69

10 57 83 62 69 30 59 11 63 74 43 61 28 62 12 53 78 41 51 0.33 32 59 0.04 13 51 83 32 60 48 59 0.75 14 55 81 40 69 47 55 0.59 15 58 78 48 62 0.18 39 69 16 60 85 35 68 33 73 17 59 86 62 75 46 54 18 64 88 42 75 38 60 19 61 82 0.09 49 78 31 66 20 58 82 61 82 0.43 41 68 21 67 85 0.45 50 67 0.12 35 62 22 67 88 40 74 34 62 23 69 81 0.75 44 75 45 61 0.38 24 67 91 54 64 0.08 42 62 0.03 25 67 89 0.01 54 79 42 68 0.02 26 59 78 0.05 52 83 32 64 27 55 78 55 83 46 71 28 59 70 0.23 51 84 35 53 29 64 86 0.90 51 81 34 50 30 63 83 0.51 46 69 31 55 31 58 86 24 43

Mean/Total 71.2 3.82 62.0 2.78 49.2 2.53 Normal 69.6 4.26 61.9 2.79 49.4 2.61 D.F.N. 1.6 -0.44 0.1 -0.01 0.2 -0.08

1Weather station located at test site. 3 Weather station located approximately 5 miles northwest of field location

49

APPENDIX III

Materials Tested

50

Table 1. Conventional Insecticides used in studies in 2014.

Table 2. Rootworm active Bt Traits

Product name

Rate

(ounces a.i. per 1000

row-feet) Active ingredient Company

Aztec 2.1G 0.14 tebupirimphos & cyfluthrin

AMVAC Chem.

Corp.

Aztec-SB 4.67G 0.14 tebupirimphos & cyfluthrin

AMVAC Chem.

Corp.

Belay 50WDG 0.10, 0.15 & 0.20 clothianidin Valent Corporation

Capture LFR 1.5SC 0.10 Bifenthrin FMC Corporation

Counter-SB 20G 0.90 Terbufos

AMVAC Chem.

Corp.

Force 3G 0.12 & 0.15 tefluthrin Syngenta

Force 250CS 0.12 tefluthrin Syngenta

SmartChoice-SB 5G 0.25 Chlorethoxyfos + bifenthrin

AMVAC Chem.

Corp.

Product name Event Toxin Company

Agrisure 3000GT

Agrisure 3111 MIR604 mCry3A Syngenta

Agrisure 3122

DAS-59122-7 +

MIR604 Cry34/35Ab1 + mCry3A Syngenta

Herculex DAS-59122-7 Cry34/35Ab1

Pioneer & Dow

AgroSciences

Smartstax

DAS-59122-7 +

MON 88017 Cry34/35Ab1 + Cry3Bb1

Dow AgroSciences

& Monsanto

VT Triple Pro MON 88017 Cry3Bb1 Monsanto

51

APPENDIX IV

Research Pictures

52

From left to right, summer 2014 undergraduate technical assistants, Anthony Blake, Eric Stuart, Cory Hermsen, Andrea D. Raygor, Andrew Guinness, Patrick Weber –supervisor (Agricultural Specialist-ISU Dept. of Entomology) and Alex Abdallah

Patrick Weber installing a Corn Rootworm emergence cage trap at a grower field location in north central Iowa

53

Jeff Erb (Combine operator) and Patrick Weber harvesting corn plots at the ISU Bruner farm location, Boone, Iowa

Dr. Siva Jakka and Patrick Weber checking the growth stage of the corn plants at a grower field in North Central Iowa

2014 iowa evaluation of insecticides and plant ...performance of registered and pre-commercial...

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