13 dioxin detection:

(dioxin): 210 75 polychlorinated dibenzo--dioxinsPCDDs135 polychlorinated dibenzofuransPCDFs 210172,3,7,8-(2,3,7,8-TCDD)

209(PCB)12, (dioxin-like congener PCB)

2,3,7,8-(2,3,7,8-TCDD)

toxic equivalent quantitiesTEQ

2,3,7,8-TCDD toxic equivalent quantities

toxic equivalent factorTEFPCDDs 2,3,7,8-TCDD PCDDs PCDFs TEF TEQTEQ

Toxic Equivalent Factors

/HRGC/HRMS2~3

Chemically Activated Luciferase Expression CALUX

CALUX

H4IIE rat-hepatomaH4IIEluciferase geneXRE

CALUX 3~4

Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence.One famous example is the firefly luciferase.In luminescent reactions, light is produced by the oxidation of a luciferin

luciferin + O2 oxyluciferin + light

luciferin + ATP luciferyl adenylate + PPi luciferyl adenylate + O2 oxyluciferin + AMP + light

Enzyme-linked immunosorbentassayELISA Electrochemical biosensor Surface plasmon resonance immunosensor Piezoelectric quartz crystal microbalancebiosensor2,3,7,8-TCDD AhR-ARNT

dioxin binding peptidepeptide library Combinatorial Chemistry

Combinatorial ChemistryMerrifield: the solid-phase synthesis of peptides.

Furka, 1988 : divide-couple-recombine, mixing-divide-coupleHoughten, 1991 : mesh packets (Nature), positional scanning