13 dioxin detection 戴奧辛 : 世紀之毒 主要由燃燒行為產生 包括廢棄物焚化等
Post on 20-Dec-2015
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TRANSCRIPT
- Slide 1
- 13 dioxin detection :
- Slide 2
- (dioxin) : 210 75 polychlorinated dibenzo- -dioxins PCDDs 135 polychlorinated dibenzofurans PCDFs 210 17 2,3,7,8- (2,3,7,8-TCDD) 209 (PCB) 12 , (dioxin-like congener PCB)
- Slide 3
- 2,3,7,8- (2,3,7,8-TCDD)
- Slide 4
- toxic equivalent quantities TEQ 2,3,7,8-TCDD toxic equivalent quantities toxic equivalent factor TEF PCDDs 2,3,7,8-TCDD PCDDs PCDFs TEF TEQ TEQ
- Slide 5
- Toxic Equivalent Factors
- Slide 6
- / HRGC/HRMS 2~3
- Slide 7
- Slide 8
- Chemically Activated Luciferase Expression CALUX CALUX H4IIE rat-hepatoma H4IIE luciferase gene XRE CALUX 3~4
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- Slide 10
- Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence. One famous example is the firefly luciferase. In luminescent reactions, light is produced by the oxidation of a luciferin luciferin + O2 oxyluciferin + light luciferin + ATP luciferyl adenylate + PPi luciferyl adenylate + O2 oxyluciferin + AMP + light
- Slide 11
- Slide 12
- Enzyme-linked immunosorbentassay ELISA Electrochemical biosensor Surface plasmon resonance immunosensor Piezoelectric quartz crystal microbalance biosensor 2,3,7,8-TCDD AhR- ARNT
- Slide 13
- dioxin binding peptide peptide library Combinatorial Chemistry
- Slide 14
- Combinatorial Chemistry Merrifield: the solid-phase synthesis of peptides. Furka, 1988 : divide- couple-recombine, mixing- divide-couple Houghten, 1991 : mesh packets (Nature), positional scanning