1260 infinity ii lc presentation - agilent.com · marketing director. agenda. 1260 infinity ii...
TRANSCRIPT
The new 1260 Infinity II LC New InfinityLab LC Series Workflow Solutions
AGILENT THE PREMIER LABORATORY PARTNER FOR A BETTER WORLD Dr. Martin Vollmer
Marketing Director
Agenda
1260 Infinity II Automated Multimethod and Method Development
1260 Infinity II Bio-inert LC
1260/1290 Infinity II GPC Solution
Pre-defined personalized methods
InfinityLab LC Solutions
Multiple users
One power user Automated method
development
1260 Infinity II (600 bar) 1290 Infinity II (1300 bar)
Method Development Solution
Multi Method Solution
One Hardware Setup
One Hardware Setup – Two Solutions
Method Scouting Wizard A.02.06 Autom. sequence setup
Runtime optimization
Reporting
…
OpenLab CDS Lab-at-a-glance view
Full compliance with GLP, GMP
Intelligent Reporting
…
Method Development
min 0.5 1 1.5 2 2.5 3 3.5
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Eclipse plus C18
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Eclipse plus PhenylHexyl
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Bonus RP
R = ¼ 𝑁(a-1)(k/[1+k])
Changes of a have a much higher effect than changes of N !
Why different separation conditions?
Hardware and Columns
Choose from our InfinityLab column and consumables repertoire to set up your method development system
Automated Method and
Sequence Setup
Use Method Scouting Wizard for automated sequence setup optimizing time and solvent consumption
Data Acquisition and Run Control
Discover reliable and convenient data acquisition using OpenLab ChemStation
Reporting and Data Processing
Discover the new Method Scouting Wizard reporting functions to create individual reports
InfinityLab Method Development Solutions Solutions that cover your complete workflow
Exploring possibilities in Method Development
Four columns in one MCT Eight columns per MCT, clustering of up to four MCT
DT
Two temperature zones per MCT, up to four MCT
DT
Two independent temperature zones
1260 Infinity II Method Development 1290 Infinity II Method Development
Column Capacity
Temperature Zones
> 600 different applicable conditions > 5400 different applicable conditions
Two solvent selection valves (26 mobile phases)
A B C D
Two solvent selection valves (26 mobile phases)
A B C D
External Solvent Selection Valves
Multicolumn Thermostat – To Serve Multiple Columns
New 1260 Infinity II Multicolumn Thermostat with simplified access, easy and flexible to install Quick Connect heat exchangers and Quick Change Valves.
Superior access to heat exchangers, valves and columns
1260 Infinity II Multicolumn Thermostat
• Usability Easily find column details
• Traceability Always know exactly which columns are installed and in use
• Security Protect against the use of methods incompatible with the column
Understand key details and record usage of the multiple columns with your LC system. Avoid mistakes and plan for exchange when needed
New column tag reader for a wide range of pre-labeled InfinityLab Poroshell columns
InfinityLab Series Improves Usability
The new Quick Change heat exchangers can be installed in
seconds by turning just two clips. There is no capillary attached
that could break.
Technology: Quick Connect heat exchangers
New concept of Multicolumn Thermostat Heat Exchangers
InfinityLab Valve Options
Technology: Quick Change Valves
Column selection valves
1260 Infinity II Method Development system comes with new 4-column selector valve – also supported by Method Scouting Wizard SW
The New Agilent 1260 Infinity II LC!
Analytical Efficiency for ultimate confidence in your data!
Analytical Efficiency – Low Dispersion Connections
Dispersion optimized: InfinityLab Quick Connect and Quick Turn fittings
not only add usability when installing columns, with their spring loaded
mechanism they also help effectively reducing dispersion.
Thus, improving chromatographic results.
Method Development Software Solutions
selected column is shown directly in the instrument control
no need to deal with individual valve positions
Column centric view for more usability
Agilent ChemStation Method Scouting Wizard
• Define project Choose scouting combinations and base method.
• Select columns All installed columns are shown automatically.
• Select solvents Pump types and valves are automatically detected.
• Define gradients Select between different gradients and temperatures.
• Review and select screening methods Check for incompatible combinations.
• Set up samples Define injection volumes and number of repetitions.
Easy and fast setup of sequences !
The easy way to set up even complex screening campaigns
With MSW A.02.06, 1290 Infinity II Method Development can also be combined with
ISET
Cross-System Method Transfer Solution
*
Flexible High Speed
1290 Infinity II LC
MSW and Intelligent System Emulation Technology (ISET)
Fast method transfer: 1) Method development on
1290 Infinity II system without ISET
2) Method translation using ISET, fine tuning on target system
Advanced method transfer: 1) Emulation of target system
using ISET 2) Method Development in
ISET mode 3) Transfer of complete
method to target system
1260 Infinity II Automated Multimethod and Method Development
1260 Infinity II Bio-inert LC
1260/ 1290 Infinity II GPC solution
New Agilent 1260 Infinity II Bio-inert LC
True bio-inertness – 100% metal-free sample flow path
New detectors (improved specifications)
New Column Compartment (ease-install bio-inert heat exchanger, Quick Connect bio-inert fittings)
New bio-inert Multisampler (ideal for microtiter plate based workflows, high capacity, faster, lowest carry-over)
New bio-inert pump (improved usability, full backwards method compatibility)
1260 Infinity II Bio-inert LC
• Universality (U)HPLC system for small- and large-molecule analysis
• Multiple Attribute Analysis Fast switching between different columns and chromatography modes
• Highly Robust Corrosion resistant and widest pH range
• Small scale purification Fraction collection up to 10 mL/min
Stainless steel system
1260 Infinity Bio-inert LC
True bio-inertness – 100% metal-free sample flow path
New Agilent 1260 Infinity II Bio-inert Multisampler
100% Bio-inert
Precious sample does not touch any metal surface
Lowest carryover
Single digit (9 ppm) using Multi-Wash feature active seat backflush
Efficient temperature control
For temperature-sensitive samples avoiding degradation
Highest capacity at lowest bench space
6144 samples within Agilent LC footprint - Single height drawers - Dual height drawers - Triple height drawers
Highest flexibility, ultralow carry-over and 100% metal free - for your bio applications
New Agilent 1260 Infinity II Bio-inert Heat Exchangers
100% Bio-inert Metal-free heat exchangers
New 1260 MCT for Multimethod Multi-Attribute Analysis
New bio-inert Quick Change 4-column selection valve Multi-attribute analysis and automated Method Development
Maximum application flexibility
Peltier cooling and heating with two independent temperature zones from 10 °C below ambient up to 85°C
Ease of use Prepared for InfinityLab Quick Connect fittings; flexible flap positions: open door to 90° (desk function), to 180° or even remove door for best accessibility
Designed to fit in new MCT
Agilent BioColumns Portfolio
Agilent Bio-LC Columns
Affinity
Bio-Monolith Protein A
Bio-Monolith Protein G
Reversed Phase
AdvanceBio Peptide Mapping ZORBAX RRHD
300A 1.8µm
Poroshell 300
AdvanceBio RP mAb
ZORBAX 300SB
ZORBAX Amino Acid Analysis
PLRP-S
AdvanceBio Oligonucleotide
HILIC
AdvanceBio Glycan Mapping ZORBAX RRHD
300-HILIC
Size Exclusion
AdvanceBio SEC
Bio SEC-3
Bio SEC-5
ProSEC 300S
ZORBAX GF-250
ZORBAX GF-450
Ion Exchange
Bio-Monolith (QA, DEAE, SO3)
Bio mAb
Bio IEX (SAX, SCX, WAX, WCX)
PL-SAX
PL-SCX
AdvanceBio SEC Delivers High Resolution of mAb Impurity Profile for Better Accuracy
AdvanceBio SEC 2.7 um 300A, 7.8 x 300 mm
Competitor A: 4um, 7.8 x 300 mm
min 5 10 15
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AdvanceBio SEC Delivers Improved Peak Shape for an ADC with New Bonded Phase
AdvanceBio SEC 2.7 um 300A
Competitor 5um,
7.8 x 300 mm
Competitor 4um, 7.8 x 300 mm
Competitor 3um, 7.8 x 300 mm
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Quaternary Buffer Mixing with Buffer Advisor Ion Exchange Chromatography
Ease of use to setup ion-exchange chromatography
What is Buffer Advisor?
• Buffer Advisor is an independent utility software to calculate pump time tables for ion-exchange chromatography with the 1260 Infinity II Bio-inert pump
• Provides the foundation for automated mixing of acid, base, water and salt in IEX
• Suggests for the most suitable stock solutions and provides recipes
• Has included more than 50 different buffers and more than 20 were experimentally validated
• Is capable to correct automatically for pH shifts during salt gradients
Ease of use to setup ion-exchange chromatography
1260 Infinity II Automated Multimethod and Method Development
1260 Infinity II Bio-inert LC
1260/ 1290 Infinity II GPC solution
The new Agilent 1260 Infinity II LC!
Significantly improved workflow solution!
New Refractive Index Detectors (higher data rate, micro-flow cell available) New Column Compartment (no problem to place long GPC columns, even couple up to four)
New Vialsampler (faster, lower carry-over)
New Isocratic Pump (solvent selection and degassing option)
1260 Infinity II GPC/SEC Solution
Instrument Control
Data Collection
GPC/SEC Calculations
• Same look-and-feel as the OpenLAB CDS ChemStation for HPLC
• Easy to move from one system to another when deploying both techniques
Powerful Agilent GPC Software
• The Agilent 1260 Infinity II MCT accommodates up to four 30 cm GPC columns
• The 1260 Infinity II Multicolumn Thermostat allows precise column thermostatting over a broad temperature independent of surroundings
• A shift in retention times related to poor temperature control could result in a change in molecular weight of many thousands of daltons
High Precision Temperature Control 1260 Infinity II Multicolumn Thermostat (MCT)
Superior temperature stability and more space to couple up to 4 long columns
Two New Refractive Index Detectors: Comparison
G7162A: Agilent 1260 Infinity II Refractive Index Detector (RID)
G7162B: Agilent 1290 Infinity II Refractive Index Detector (RID)
Replaces the current model, G1362A with higher data rate and “RoHS compliant”
A new product introduction with a small volume cell and higher data rate
Increase GPC Productivity with the 1290 Infinity II RID
Epoxy resin
Achieve shorter run times:-
1.2ml/min
0.3ml/min
0.6ml/min
2.5 µL low volume flow cell Maximum data rate of 148 Hz Narrow internal diameter columns (4.6 x 250mm)
Resolution maintained at a fourth of run time
Case Study – Customer Sample “Kraton”
Sta
50min
Standard GPC columns & Conventional RI Customer wanted to reduce
runtime and maintain resolution
Narrow-bore GPC columns & Conventional RI
Loss of resolution with shorter run time & conventional RID
Resolution achieved using standard GPC columns & micro RID
Narrow-bore GPC columns & Micro RID
15min
4 columns individual pore
sizes 7.5mmx300mm
2 columns ResiPore
4.6mmx250mm
2 columns ResiPore
4.6mmx250mm
µRID
Standard RID
15min
Standard RID
More resolution for polymer samples
A comprehensive GPC solution, the complete workflow for polymer characterization supplied and supported by Agilent
New Agilent 1260 Infinity II GPC!
Thanks for your attention!!!!