0 4 8 12 24 48 (hr) cleaved caspase-9 caspase-8 100 nm paclitaxel mcf-7 figure s1a
DESCRIPTION
Paclitaxel 100 nM Bad siRNA Scrambled siRNA Bad Actin IP: Bcl-2 IB: Bcl-2 Input IB: Bcl-2 Bcl-2 Actin IP: Bcl-2 IB: Bim BimEL BimL BimS MCF-7 Figure S1cTRANSCRIPT
![Page 1: 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel MCF-7 Figure S1a](https://reader036.vdocuments.site/reader036/viewer/2022062317/5a4d1b1b7f8b9ab05999365d/html5/thumbnails/1.jpg)
0 4 8 12 24 48 (hr)
Cleaved Caspase-9
Caspase-8
100 nM Paclitaxel
0
2
4
6
8
Control
Paclita
xel 1
00 nM
RFU
(Arb
itrar
y U
nits
)
Caspase-9 activity Caspase-8 activity
MCF-7
0
10
20
30
40
50
60
70
80
90
100
Control
Paclita
xel
Z-VAD-FMK + Paclita
xel
Z-IETD-FMK + Pac
litaxe
l
Z-LEHD-FMK + Pac
litaxe
l
% A
nnex
in V
-pos
itive
cel
ls
Figure S1a
![Page 2: 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel MCF-7 Figure S1a](https://reader036.vdocuments.site/reader036/viewer/2022062317/5a4d1b1b7f8b9ab05999365d/html5/thumbnails/2.jpg)
MCF-7
Figure S1b
0102030405060708090
100
Control
Paclita
xel
Bim si
RNA
Paclita
xel +
Bim
siRNA
Paclita
xel +
ABT-73
7+ B
im siR
NA
Bmf siR
NA
Paclita
xel +
Bmf s
iRNA
Paclita
xel +
ABT-73
7+ B
mf siR
NA
ABT-737
ABT-737 +
Bim
siRNA
ABT-737 +
Bmf s
iRNA
Paclita
xel +
ABT-73
7
% A
nnex
in V
-pos
itive
cel
ls
***
![Page 3: 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel MCF-7 Figure S1a](https://reader036.vdocuments.site/reader036/viewer/2022062317/5a4d1b1b7f8b9ab05999365d/html5/thumbnails/3.jpg)
- - + + - -
- + - + - +Paclitaxel 100 nM
Bad siRNA
Scrambled siRNA - - - - + +
Bad
Actin
IP: Bcl-2
IB: Bcl-2
Input
IB: Bcl-2
Bcl-2
Bcl-2
Actin
IP: Bcl-2
IB: Bim
BimELBimLBimS
MCF-7
Figure S1c
![Page 4: 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel MCF-7 Figure S1a](https://reader036.vdocuments.site/reader036/viewer/2022062317/5a4d1b1b7f8b9ab05999365d/html5/thumbnails/4.jpg)
0 4 8 12 24 48 (hr)
Cleaved Caspase-9
Caspase-8
100 nM Paclitaxel
Cleaved Caspase-3
0
2
4
6
8
RFU
(Arb
itrar
y U
nits
)
Caspase-9 activity Caspase-8 activity Caspase-3 activity
T47D
0
10
20
30
40
50
60
70
80
90
100
Control
Paclita
xel
Z-VAD-FMK + Paclita
xel
Z-IETD-FMK + Pac
litaxe
l
Z-LEHD-FMK + Pac
litaxe
l
% A
nnex
in V
-pos
itive
cel
ls
Figure S1d
![Page 5: 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel MCF-7 Figure S1a](https://reader036.vdocuments.site/reader036/viewer/2022062317/5a4d1b1b7f8b9ab05999365d/html5/thumbnails/5.jpg)
0 4 8 12 24 48 (hr)
Cleaved Caspase-9
Caspase-8
100 nM Paclitaxel
Cleaved Caspase-3
0
2
4
6
8
RFU
(Arb
itrar
y U
nits
)
Caspase-9 activity Caspase-8 activity Caspase-3 activity
MDA-MB-468
0
10
20
30
40
50
60
70
80
90
100
Control
Paclita
xel
Z-VAD-FMK + Paclita
xel
Z-IETD-FMK + Pac
litaxe
l
Z-LEHD-FMK + Pac
litaxe
l
% A
nnex
in V
-pos
itive
cel
ls
Figure S1e
![Page 6: 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel MCF-7 Figure S1a](https://reader036.vdocuments.site/reader036/viewer/2022062317/5a4d1b1b7f8b9ab05999365d/html5/thumbnails/6.jpg)
0 4 8 12 24 48 (hr)
Cleaved Caspase-9
Caspase-8
100 nM Paclitaxel
Cleaved Caspase-3
0
2
4
6
8
RFU
(Arb
itrar
y U
nits
)
Caspase-9 activity Caspase-8 activity Caspase-3 activity
BT20
0
10
20
30
40
50
60
70
80
90
100
Control
Paclita
xel
Z-VAD-FMK + Paclita
xel
Z-IETD-FMK + Pac
litaxe
l
Z-LEHD-FMK +
Paclita
xel
% A
nnex
in V
-pos
itive
cel
ls
Figure S1f
![Page 7: 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel MCF-7 Figure S1a](https://reader036.vdocuments.site/reader036/viewer/2022062317/5a4d1b1b7f8b9ab05999365d/html5/thumbnails/7.jpg)
IP: Bcl-xL
IB: Bcl-xLBcl-xL
Bim ELBim LBim S
IP: Bcl-xL
IB: Bim
Mcl-1
Paclitaxel 100 nM
0 4 8 12 [h]
Bim ELBim LBim S
IP: Mcl-1
IB: Bim
Paclitaxel 100 nM
0 4 8 12 [h]
IP: Mcl-1
IB: Mcl-1
Figure S2a
MCF-7
![Page 8: 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel MCF-7 Figure S1a](https://reader036.vdocuments.site/reader036/viewer/2022062317/5a4d1b1b7f8b9ab05999365d/html5/thumbnails/8.jpg)
Figure S2b
Pre-
IP
Post
-IP
BimELBimLBimS
HM LM S-100
Untreated Taxol 12 h
CoxIV
BiP/Grp78
BimELBimL
BimS
1 0.43
Actin
Ratio
Paclitaxel 100 nM
- +
Pre-
IP
Post
-IP
Actin
BimELBimLBimS
Untreated
1 1.39Ratio
HM LM S-100
MCF-7 MCF-7MCF-7
![Page 9: 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel MCF-7 Figure S1a](https://reader036.vdocuments.site/reader036/viewer/2022062317/5a4d1b1b7f8b9ab05999365d/html5/thumbnails/9.jpg)
- + - +
Paclitaxel 100 nM
MCF-7 MCF-7 Bcl-2+
FLAG-Bcl-2
Bcl-2FLAG-Bcl-2
BimEL
BimL BimS
IP: FLAG
IB: Bcl-2
Input
IB: Bcl-2
IP: FLAG
IB: Bim
Bmf
Bmf
IP: FLAG
IB: Bmf
Input
IB: Bmf
BimELBimL
BimSInput
IB: Bim
Actin
IP: FLAG
IB: Bax
IP: FLAG
IB: Puma
*
IP: FLAG
IB: Bak
IP: FLAG
IB: Bad
- + - +
Paclitaxel 100 nM
MCF-7 MCF-7 Bcl-2+
Bad
Puma
Bax
Bak
Figure S2c
![Page 10: 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel MCF-7 Figure S1a](https://reader036.vdocuments.site/reader036/viewer/2022062317/5a4d1b1b7f8b9ab05999365d/html5/thumbnails/10.jpg)
Figure S2d
IP: Bcl-2
IB: BaxBax
IP: Bcl-2
IB: Bak
Paclitaxel 100 nM
- +
IP: Bcl-xL
IB: Bax
IP: Bcl-xL
IB: Bak
Paclitaxel 100 nM
- +
IP: Mcl-1
IB: Bax
IP: Mcl-1
IB: Bak
Paclitaxel 100 nM
- +
Bak
Bax
Bak
Bax
Bak
MCF-7
![Page 11: 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel MCF-7 Figure S1a](https://reader036.vdocuments.site/reader036/viewer/2022062317/5a4d1b1b7f8b9ab05999365d/html5/thumbnails/11.jpg)
0 4 8 12 24 48 0 4 8 12 24 48 (hr)
MitoCyto
Cytochrome c
CoxIV
T47D
Figure S3a
![Page 12: 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel MCF-7 Figure S1a](https://reader036.vdocuments.site/reader036/viewer/2022062317/5a4d1b1b7f8b9ab05999365d/html5/thumbnails/12.jpg)
0 4 8 12 24 48 0 4 8 12 24 48 (hr)
MitoCyto
MDA-MB-468
Figure S3b
Cytochrome c
CoxIV
![Page 13: 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel MCF-7 Figure S1a](https://reader036.vdocuments.site/reader036/viewer/2022062317/5a4d1b1b7f8b9ab05999365d/html5/thumbnails/13.jpg)
0 4 8 12 24 48 0 4 8 12 24 48 (hr)
MitoCyto
BT20
Figure S3c
Cytochrome c
CoxIV
![Page 14: 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel MCF-7 Figure S1a](https://reader036.vdocuments.site/reader036/viewer/2022062317/5a4d1b1b7f8b9ab05999365d/html5/thumbnails/14.jpg)
0 4 8 12 24 48 (hr)
Bax
Bak
IP: Bax 6a7
IB: Bax N20
IP: Bak Ab-2
IB: Bak G23
100 nM Paclitaxel
T47D
Input
IB: Bax N20
Input
IB: Bak G23
Bax
Bak
Actin
Figure S4a
![Page 15: 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel MCF-7 Figure S1a](https://reader036.vdocuments.site/reader036/viewer/2022062317/5a4d1b1b7f8b9ab05999365d/html5/thumbnails/15.jpg)
0 4 8 12 24 48 (hr)
Bax
Bak
IP: Bax 6a7
IB: Bax N20
IP: Bak Ab-2
IB: Bak G23
100 nM Paclitaxel
MDA-MB-468
Input
IB: Bax N20
Input
IB: Bak G23
Bax
Bak
Actin
Figure S4b
![Page 16: 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel MCF-7 Figure S1a](https://reader036.vdocuments.site/reader036/viewer/2022062317/5a4d1b1b7f8b9ab05999365d/html5/thumbnails/16.jpg)
0 4 8 12 24 48 (hr)
Bax
Bak
IP: Bax 6a7
IB: Bax N20
IP: Bak Ab-2
IB: Bak G23
100 nM Paclitaxel
BT20
Input
IB: Bax N20
Input
IB: Bak G23
Bax
Bak
Actin
Figure S4c
![Page 17: 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel MCF-7 Figure S1a](https://reader036.vdocuments.site/reader036/viewer/2022062317/5a4d1b1b7f8b9ab05999365d/html5/thumbnails/17.jpg)
Puma
Actin
- - + + - -
- + - + - +Paclitaxel 100 nM
Puma siRNA
Scrambled siRNA - - - - + +
MDA-MB-468
0
10
20
30
40
50
60
70
80
90
100
Control
Paclita
xel
Puma siR
NA
Paclita
xel +
Pum
a siR
NA
Scram
bled s
iRNA
Paclita
xel +
Scra
mbled si
RNA
% A
nnex
in V
-pos
itive
cel
ls
Figure S5a
![Page 18: 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel MCF-7 Figure S1a](https://reader036.vdocuments.site/reader036/viewer/2022062317/5a4d1b1b7f8b9ab05999365d/html5/thumbnails/18.jpg)
Puma
Actin
- - + + - -
- + - + - +Paclitaxel 100 nM
Puma siRNA
Scrambled siRNA - - - - + +
BT20
0
10
20
30
40
50
60
70
80
90
100
Control
Paclita
xel
Puma siR
NA
Paclita
xel +
Pum
a siR
NA
Scram
bled s
iRNA
Paclita
xel +
Scra
mbled si
RNA
% A
nnex
in V
-pos
itive
cel
ls
Figure S5b
![Page 19: 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel MCF-7 Figure S1a](https://reader036.vdocuments.site/reader036/viewer/2022062317/5a4d1b1b7f8b9ab05999365d/html5/thumbnails/19.jpg)
Puma
Actin
- - + + - -
- + - + - +Paclitaxel 100 nM
Puma siRNA
Scrambled siRNA - - - - + +
MCF-7
0
10
20
30
40
50
60
70
80
90
100
Control
Paclita
xel
Puma siR
NA
Paclita
xel +
Pum
a siR
NA
Scram
bled s
iRNA
Paclita
xel +
Scra
mbled si
RNA
% A
nnex
in V
-pos
itive
cel
ls
Figure S5c