魏炽炬 epithelial–mesenchymal transition. origin of epithelium and mesenchyme there are two...
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魏炽炬
Epithelial–Mesenchymal Transition
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Origin of Epithelium and Mesenchyme
• There are two major types of cell arrangements in the embryo:
• epithelial cells, which are tightly connected to one another in sheets or tubes,
• and mesenchymal cells, which are unconnected to one other and operate as independent units.
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Origin of Epithelium and Mesenchyme
• Epithelia are sheets or tubes of connected cells; they can originate from any germ layer.
• Meschymal cells are loosely packed unconnected cells; mesenchyme contains collagen bundles and fibroblasts; they are derived from the mesoderm or neural crest.
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In the Adult
• Epithelium is a tissue composed of cells that line the cavities and surfaces of structures throughout the body.
• Epithelium is classified as a primary body tissue, the other ones being connective tissue, muscle tissue and nervous tissue.
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In the Adult
• Mesenchyme later differentiates into blood vessels, blood-related organs, and connective tissues.
• All organs consist of an epithelium and an associated mesenchyme.
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Epithelial–Mesenchymal Transition
Hartmut Beug
Actin/
DAPI
E-cadherin
Vimentin
Desmosomes
Vimentin
Epithelial
Mesenchymal
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Epithelial–Mesenchymal TransitionJean Paul Thiery
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EMT in Tissue RemodellingJean Paul Thiery
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The generation of three germ layers
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下胚层
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The migration of neural crest
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Somitogenesis
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The generation of glomerulus
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EMT During Tumor ProgressionHartmut Beug
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Paper One
Epithelial-to Mesenchymal Transition Generates Proliferative Human Islet Prec
ursor Cells
• Marvin Gershengorn, Anandwardhan Hardikar, Chiju Wei, Elizabeth Geras-Raaka, Bernice Marcus- Samuels, Bruce M. Raaka• SCIENCE VOL 306, 2004
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Embryonic StemEmbryonic StemCellCell
Bone MarrowBone MarrowStem CellStem Cell
TransdifferentiationTransdifferentiation
Pancreatic stem Pancreatic stem cellcell
Cadaveric hCadaveric human isletsuman islets
Potential Sources of Islet Precursor CellsPotential Sources of Islet Precursor Cells
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hIPC Generation and ICA Formation hIPC Generation and ICA Formation
A B C
1 hr 24 hr 6 day
D E F
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Transition From Insulin+ to VimentiTransition From Insulin+ to Vimentin+ Cells n+ Cells
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Redifferentiation From Vimentin+ to Redifferentiation From Vimentin+ to Insulin+ Cells Insulin+ Cells
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Islet Precursors Generated by EMT Islet Precursors Generated by EMT Marvin Gershengorn
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EMT in Progenitor Cells of The Developing Endocrine Pancreas
Joel F. Habener
EpitheliumEpithelium
Basel membraneBasel membrane
ECMECM
Acinar cellAcinar cell
Islet
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Paper Two
Islet-Derived Fibroblast-Like Cells Are Not Derived via Epithelial-Mesenchymal Transition From Pdx-1 or Insulin-Positive
Cells
• Lucas G. Chase, Fernando Ulloa-Montoya, Benjamin L. Kidder, and Catherine M. Verfaillie• Diabetes 56:3–7, 2007
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Lineage Tracing on Proliferative Fibroblast-like Cell Populations
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Fibroblast-like Cells Are Not Generated From Insulin+/PDX1+ Cells
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About 25% of All Insulin-Expressing Cells Coexpressed EGFP
Nadya L. Lumelsky
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Paper Three
In Vitro Proliferation of Cells Derived From Adult Human -Cells Revealed By Cell-
Lineage Tracing
• Holger A. Russ, Yael Bar, Philippe Ravassard, and Shimon Efrat• Diabetes 57, 2008
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In Vitro Lineage Tracing of Islet Beta Cells
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Labeling of human islet cells with the two-virus system