http://www.youtube.com/watch?v=bk7PW1FKMTI&feature=player_embedded

真核生物の転写と翻訳の動画 youtubeから

FIGURE 2 | Promoter-proximal pausing and escape to productive elongation.From the following article:

Breaking barriers to transcription elongationAbbie Saunders, Leighton J. Core & John T. LisNature Reviews Molecular Cell Biology 7, 557-567 (August 2006)

Polyclonal Antibodies

http://www.gelifesciences.co.jp/newsletter/biodirect_mail/technical_tips/tips63.asp

Monoclonal Antibodies

- - Screening the logic:

Unfused myeloma cells cannot grow because they lack HGPRT. Unfused normal spleen cells cannot grow indefinitely because of their limited life span. However, Hybridoma cells (produced by successful fusions) are able to grow indefinitely because the spleen cell partner supplies HGPRT and the myeloma partner is immorta

(http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/M/Monoclonals.html)

脾臓

ミエローマ

Western Blotting

1st Ab

2nd Ab

http://www.motifolio.com/6111115.html

2nd Abの例AP-labeledgoat anti-rabbit IgG

IR Dye 800-labeledgoat anti-rabbit IgG(Figure 2)

組換えタンパク質の TagTagによる検出

Tagによる精製

Tagによる相互作用解析

http://www.act-nonpareil.com/products/pro2.html

免疫沈降法 (immunoprecipitation： IP)

PF0099

LSU ribosomal protein L12A

50S ribosomal protein L31e

50S ribosomal protein L21e

50S ribosomal protein L14e

50S ribosomal protein L7Ae

30S ribosomal protein S6e

50S ribosomal protein L37e

30S ribosomal protein S8P

50S ribosomal protein L24e

PF0027と相互作用解析するタンパク質とMSによる分子同定（ feat. Mr. Masuda)

クロマチン免疫沈降法