your new alternative to lc-ms - 908 devices...there’s no labeling or derivatization. small polar...

Your new alternative to LC-MS

MASS SPEC COMPATIBLE CHARGE VARIANT ANALYSIS

Many current mAb characterization techniques either provide high-resolution charge variant separations

or mass spec data. Not both. This makes identification of mAb variants difficult.

No more either/orZipChip® can perform high-resolution charge variant separations of intact antibodies backed by the power

of Native-MS characterization. In a single run you can get mass information, assess charge heterogeneity,

characterize glycoforms, identify basic and acidic variants, and more. The added layer of mass spec

information means you can finally pinpoint the identity of each peak.

Get the unfair advantageWhen compared to traditional methods for charge variant analysis of antibodies, ZipChip separates more

and allows mass spec identification for each charge variant.

Charge Variant Separation

MS CompatibleCharge Variant

MassesCharge Variant ID

ZipChip HHHHH HHHHH HHHHH HHHHH

Isoelectric focusing HHHHH HH

CE (UV) HHHHH HH

RPLC-MS H HHHHH HH HH

APPLICATION SNAPSHOT

Charge Variant Analysis

Z I P C H I P I S F O R R E S E A R C H U S E O N LY ( R U O )

Charge variant analysis of NIST mAb

Charge variant analysis of NIST mAb stressed at pH 8 for 72 hours

Kit Type: Native Antibody

Chip Type: ZipChip HRN

Sample Prep: Dilution from formulation. Buffer exchange may be necessary

MS Type: Thermo Scientific Orbitraps with Biopharma or EMR options

Native Mass Spectrum

+26 Charge State

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Charge Variant Analysis

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Z I P C H I P I S F O R R E S E A R C H U S E O N LY Zip Chip is subject to export controls including those of the Export Administration Regulations of the U.S. Department of Commerce, which may restrict or require licenses for the export of product from the United States and their re-export to and from other countries. Patented technology www.908devices/patents © 2019 908 Devices

WHIZ THROUGH INTACT MASS


Intact Mass


Intact mass analysis is routinely used to characterize proteins and provide sequence confirmation. The

system used for this analysis should be high-throughput, simple, and tolerant of many different sample

matrices so that sample prep is easy, and data is generated quickly.

Accelerate intact analysisZipChip® provides a fast, accurate, and flexible approach to intact mass analysis. Rapidly screen for the

ultimate in high-throughput analysis, or tailor the method to suit a flexible mAb characterization approach

without having to change conditions or instrumentation. Queue it all up, ignore your salt and polymeric

buffer components, and hit go.

Get the unfair advantageWhen compared to traditional methods for intact mass determination, ZipChip exploits the advantages

of microfluidic CE-MS to provide a sensitive, very simple, highly flexible approach to getting the answer

you need in less time with less hassle.

Speed of Sample Prep

Speed of Analysis

Detergents

Allowed

Compatible w/ Formulation Buffer

Compounds

ZipChip MS HHHHH HHHHH HHHHH HHHHH

MALDI-TOF H HHHHH HH H

RP-LC/MS HHHH HHH H HHHH

CE-MS HH HH — HH

Intact Mass

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High Throughput Intact Mass

Run Times: Less than 1 minute

MS Types: Thermo QE-HF and Fusion Lumos

Chip Type: ZipChip HS

Kit Type: ZipChip Peptide Kit

High Res Data

NIST mAbZipChip HS - 1000 V/cm Peptide BGE

NIST mAbZipChip HR - 500 V/cm

Peptide BGE

Standard Intact Mass

Run Times: Less than 3 minutes

MS Types: All mass spectrometers capable of intact mass analysis

Chip Type: ZipChip HR

Kit Type: ZipChip Peptide Kit

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ACCELERATE CHARGE VARIANT ANALYSIS


High Throughput Charge Variant Analysis


Charge variant analysis is routinely used to characterize proteins and provide critical information

on the charge heterogeneity of recombinant protein. The system used for this analysis should be high

throughput, simple, and able to tolerant many different sample matrices so that sample prep is easy, and

data is generated quickly.

Accelerate charge variant analysisZipChip® provides a fast, accurate, and flexible approach to charge variant analysis. Rapidly screen for

the ultimate in high-throughput analysis, or tailor the method to suit a flexible mAb characterization

approach without having to change conditions or instrumentation. Queue it all up, ignore your salt and

polymeric buffer components, and hit go.

Get the unfair advantageCurrent methodologies for charge variant analysis are typically done with conventional capillary-based

CE systems using UV detection. ZipChip can not only perform native charge variant analysis coupled to

high resolution mass spectrometry, but now has the added capability of choosing between high resolution

OR high throughput approaches.

Intact mAb ChargeVariant Separations

Native ESI-MS Spectra

Superior SpeedSuperior Separation

Resolution

HSN

HRN


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Run Times: As fast as 2 minutes

Chip Type: ZipChip HSN

Kit Type: Native Antibodies Kit

Standard Charge Variant Analysis

Run Times: About 10 minutes (molecule dependent)

Chip Type: ZipChip HRN

Kit Type: Native Antibodies Kit

ZipChip® can be used to perform an intact charge variant assay under native-ESI conditions. These

conditions generate an intact mass spectrum suitable for characterizing the intact mass and major

(charged) modifications to the protein, including deamidation. By changing the type of chip used, the

speed of the analysis can be easily tailored to the best suit the experiment and MS instrument used.

NIST mAbZipChip HSN – 1000 V/cm

NIST mAbZipChip HRN – 500 V/cm

GIVE YOUR WORKFLOW SOME ZIP

Analysis of amino acids during spent media analysis can provide important metabolic information about

the cell culture’s health, but it is difficult to do using conventional techniques. Current methods for cell

culture media analysis are slow, involve complicated sample preparation (e.g., derivatization), and can be

expensive.

Give your workflow some zipCell culture analysis is easy and fast with ZipChip®. Samples are prepared through a simple dilution and

there’s no labeling or derivatization. Small polar analyte assays take as little as 2 minutes. Add internal

standards for full quantitation on a variety of analytes.

Get the unfair advantageWhen compared to traditional methods for cell culture analysis of antibodies, ZipChip provides the only

dilute-and-shoot, label-free analysis that is fast and simple to use.

Simple Sample Prep Label Free Fast Analysis

ZipChip HHHHH HHHHH HHHHH

HPLC/UPLC H H HH

LC-MS HHH HHH HHH

GC H H H



Cell Culture Analysis

ZipChip separation of spent growth media

The amino acids migrate through the chip in less than 3 minutes. Isomers, such as isoleucine and leucine, are resolved and detected as separate peaks for confident identification and quantitation.

RT :0.00 - 3.00

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Isoleucine

LeucineRT :1.33 - 1.62

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Characterize the components in your growth media, or collect timepoints during cell culture to track nutrient metabolism and optimize growth.

Cell Culture Analysis

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SPECIFICATIONS

Your mass spec’s sidekick.

H A R D W A R E S P E C S H E E T

Put Your Mass Into It

Specifications

Sample Format Vials or standard well plates (47 mm max height)

Sample Capacity Two sample trays that hold 48 vial racks or 96-well plates

Sample Tray Cooling• Integrated Peltier Cooling • Range: 4 ºC to ambient – 3 ºC (at 80% relative humidity and 25 ºC ambient temperature)

Dimensions 14 in (h) x 12 in (w) x 22 in (d)

Weight 46.3 lbs

Power Requirements 110/240V

Specifications

Software• Standalone ZipChip acquisition software • Integrates with your MS software

Dimensions 8 in (h) x 8 in (w) x 11 in (d)

Weight 16 lbs

Power Requirements 110/240V

Laser Type Class 1 Laser Product

MS Compatibility

Thermo Scientific

• Orbitrap Exactive and Q Exactive™ Series • Orbitrap Fusion Lumos Tribrid• Orbitrap Exploris 480• Orbitrap Eclipse Tribrid LTQ and Orbitrap Elite Series• TSQ2 Series Triple Quads

SCIEX• QTRAP® 6500+, 6500, 5500, 4500 • Triple Quad 6500+, 6500, 5500, 4500, 3500 • TripleTOF 6600+, 6600, 5600

BRUKER

• compact™

• impact™

• maXis™

• timsTOF (pro)

The Interface

The compact interface seamlessly integrates all fluidics and electronics. Just clip it on your mass spec and off you go!

Optional Autosampler

Achieve unattended sample runs from sample vials or 96-well plates. The platform allows you to choreograph sample runs using ZipChip software.

ZipChip is for Research Use Only (RUO)

H A R D WA R E S P E C S H E E T

ZipChip is subject to export controls including those of the Export Administration Regulations of the U.S. Department of Commerce, which may restrict or require licenses for the export of product from the United States and their re-export to and from other countries. Patented technology www.908devices/patents. © 2020 908 Devices.

ZipChip [email protected] | www.908devices.com

Put Your Mass Into It

Consumables to simplify your workflow.

C O N S U M A B L E S S P E C S H E E T

ACTUAL SIZE

C O N S U M A B L E S S P E C S H E E T

Capacity 625 samples per kit

Contents5x 125 mL bottles of background electrolyte 5x 15 mL bottles of sample diluent

UsageCompatible with manual and autosampler ZipChip interface types.

Storage Conditions Room temperature, 10-40 ºC

The Kits

Premixed background electrolyte and sample diluent reduce preparation time for your samples. With ZipChip you just dilute and shoot!

Available Kits

Native Antibodies: Excellent charge variant separation of antibodies and other constructs to generate native spectra.

Charge Variant TOF: Formulated to provide excellent charge variant separations and MS sensitivity for antibodies and other constructs on time-of-flight mass spectrometers.

Intact Antibody: Fast, high sensitivity separations of intact antibodies without full denaturation.

Peptides: Fast, efficient separations of protein digests and peptides utilizing low pH.

Metabolites: Streamlined sample prep and high-speed analysis for any size molecule in denaturing conditions at low pH.

The Chips

Choose from these chip options to optimize your workflow. Each chip can be used for up to 125 injections. Available Chips

HR: Our standard High Resolution chip for applications demanding maximum separation performance for analytes such as large molecules and many more. To be paired with the Intact Antibody Kit, Peptides Kit, or Metabolites Kit.

HS: Our standard High Speed chip to give you the fastest separations for applications such as small molecules, metabolites and other applications where throughput is critical. To be paired with the Intact Antibody Kit, Peptides Kit, or Metabolites Kit.

HRN: A twist on our HR chip that provides ultimate performance for native protein analysis. To be paired with the Native Antibodies Kit and Charge Variant TOF Kit.

HSN: The High Speed version of our native chip for high throughput analysis of proteins analyzed under native conditions. To be paired with the Native Antibodies Kit and Charge Variant TOF Kit.

HRX and HSX: Double the run count of our standard HR and HS chips. Use these chips for up to 250 injections.

ZipChip is subject to export controls including those of the Export Administration Regulations of the U.S. Department of Commerce, which may restrict or require licenses for the export of product from the United States and their re-export to and from other countries. Patented technology www.908devices/patents. © 2020 908 Devices.

ZipChip [email protected] | www.908devices.com

ZipChip is for Research Use Only (RUO)

your new alternative to lc-ms - 908 devices...there’s no labeling or derivatization. small polar...

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