yong huang department of mechanical engineering clemson university, clemson, sc scaffold-free...
TRANSCRIPT
Yong HuangDepartment of Mechanical
Engineering
Clemson University, Clemson, SC
Scaffold-free Alginate Tube Fabrication using Inkjet and Laser printing
Table of Contents Introduction Background Objective Research Tasks
Tube Inkjetting Tube Laser Printing
Conclusions Future Work
2
Table of Contents Introduction Background Objective Research Tasks
Tube Inkjetting Tube Laser Printing
Conclusions Future Work
3
Background Inkjet-based
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[Boland, 2007]
Inkjetting of CaCl2 into 2% alginic acid to make a branched structure
[Nakamura, 2008]
Inkjetting 0.8% sodium alginate into
2% CaCl2 to make a tube with the help of gravity
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[Skardal, 2010]
Printing of agarose and 3T3 cell-containing PEGTA filaments to make a tube
[Norotte, 2009]
Printing of agarose and smooth muscle cell (SMC) cylinders to make a tube
Background Filament-based
Background Laser-based
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Laser printing of alternating layers of fibroblasts (green) and keratinocytes (red) to form a grid structure (Scale bar = 500 µm)
[Koch, 2012]
Challenges
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How to scale-up scaffold-free bioprint complex structures such as overhang structures?
How to evaluate and mitigate the post-printing cell damage?
Table of Contents Introduction Background Objective Research Tasks
Tube Inkjetting Tube Laser Printing
Conclusions Future Work
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Objective
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Needs in cellular tube bioprintingVascularization as a main technological barrier for building
3D organs
Tube printing - a logical initial step towards vascularization
Objective
To scaffold-free fabricate viable cellular tubes using inkjet and laser printing
Table of Contents Introduction Background Objective Research Tasks
Tube Inkjetting Tube Laser Printing
Conclusions Future Work
10
Table of Contents Introduction Background Objective Research Tasks
Tube Inkjetting Tube Laser Printing
Conclusions Future Work
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Liquid levelFirst layerSecond layer
3T3 cell suspension
Tube Inkjetting Experimental setup
Tube Inkjetting - Zig-zag tube
Fabrication process: bottom part, overhang part and top part
Wall thickness: 150~200 µm
Tube diameter: 3 mm and length: 10 mm
Zigzag tube fabrication
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Tube Inkjetting - Cell viability test
(a) Printed tube, (b) tube surface and (c) cell viability test after liquefying
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(a)
Cells
(b)
Live Cell
Dead Cell
After liquefying
(c)
Cell viability immediately after printing: > 85% Cell viability after 3 days: > 80% Cell viability comparable to that of the control
Tube Inkjetting - Cell viability
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0 24 48 7270
75
80
85
90
95
Cel
l via
bilit
y %
Post-printingControl
(Hours)
Tube Inkjetting - Other structures
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Pyramid Cone
Table of Contents Introduction Background Objective Research Tasks
Tube Inkjetting Tube Laser Printing
Conclusions Future Work
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Tube laser printing - Experimental setup
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ArF laser
Pulsed laser beam
Ribbon
Forming jet/droplet
Quartz support
Coating
Container
Optical table
Laser-induced vapor/plasma
3-axis stages
Moving platform
Tube laser printing - Experimental setup
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Direct-writing height = 1 mm
ArF laser
Pulsed laser beam
Ribbon
Forming jet/droplet
Quartz support
Coating
Container
Optical table
Laser-induced vapor/plasma
3-axis stages
Moving platform
Tube laser printing - results
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Top viewSide view
Φ=3 mm
Table of Contents Introduction Background Objective Research Tasks
Tube Inkjetting Tube Laser Printing
Conclusions Future Work
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Overhang structures can be scaffold-free fabricated
Viable cellular tubes (3T3) can be printed
ConclusionsInkjetting
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Tubes can be scaffold-free fabricated using LIFT-based laser printing technology
Highly viscous materials (alginate) can be laser printed into well-defined tubular structures
Laser printing
Table of Contents Introduction Background Objective Research Tasks
Tube Inkjetting Tube Laser Printing
Conclusions Future Work
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Laser printing of 3D cellular tubes
Printing of adipose-derived stem cell tubes
Future WorkFabrication
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Apoptosis/necrosis test, cell proliferation capacity, and phenotype variation
Post-printing fusion of tubular tissue
Mechanical property measurement of fabricated tubes
Post-printing evaluation
Thanks
and questions?
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