year: first year semester: i course: microbial diversity and ......year: first year course:...
TRANSCRIPT
Year: First Year
Course: Microbial Diversity and Taxonomy
Teaching
Scheme
(Hrs/Week)
Continuous Internal Assessment
L T P C CIA-1 CIA-2
4 0 - 4 10 20
Max. Time, End Semester Exam (Theory)
Prerequisite • Introduction
Objectives
1 To expand knowledge on Evolution and microbial diversity
2 To understand bacterial taxonomy
3 To study various classes of fungi in terms of their characteristic features.
4 To assimilate the concepts of unculturable bacterial diversity
5 To expand knowledge on Evolution and microbial diversity
Unit
Number
1
Differences in concept
Definition of species in prokaryotes.
Types of ‘species’
Evolution of species and concepts of speciation (in sexual
organisms)
Types of evolution (neutral, co
k selection; molecular clocks; phylogeny
2
The expanse of microbial diversity
Estimates of total number of species
Species Divergence and the measurement of microbial diversity.
Measures and indices of diversity
3
Introduction to Bacterial Taxonomy
The 5-Kingdom classification system
The 3-Domain classification system
Bergey’s Manuals and the classification of prokaryotes.
Determinative Bacteriology (Phenetic Approach)
Systematic Bacteriology (Phylogenetic
Polyphasic Approach
School of Science M.Sc. Microbiology
Semester: I
Microbial Diversity and Taxonomy Course Code:
Continuous Internal Assessment (CIA) End Semester
Examination
2 CIA-3 CIA-4 Lab Theory Lab
10 10 - 50 -
End Semester Exam (Theory) - 3Hrs.
Introduction to microbiological concepts, terms etc.
To expand knowledge on Evolution and microbial diversity.
To understand bacterial taxonomy.
study various classes of fungi in terms of their characteristic features.
To assimilate the concepts of unculturable bacterial diversity.
To expand knowledge on Evolution and microbial diversity.
Details
Differences in concept of ‘species’ in eukaryotes and prokaryotes.
Definition of species in prokaryotes.
Evolution of species and concepts of speciation (in sexual and asexual
Types of evolution (neutral, co-evolution); Types and levels of selectio
k selection; molecular clocks; phylogeny and molecular distances
The expanse of microbial diversity
Estimates of total number of species
Species Divergence and the measurement of microbial diversity.
Measures and indices of diversity
Introduction to Bacterial Taxonomy
Kingdom classification system
Domain classification system
Bergey’s Manuals and the classification of prokaryotes.
Determinative Bacteriology (Phenetic Approach)
Systematic Bacteriology (Phylogenetic Approach
Pa
ge1
Semester: I
Course Code: PMI101
Total
100
Hours
and asexual
of selection; r and
12 L
12 L
12 L
Course Outcome
Students should able to
CO1 Student will be able to understand the concepts
nomenclature.
CO2 Student will be able to
microorganisms from variety of
CO3 Student will be able to
methods.
CO4 Student will be able to
microbial species from different sources.
CO5 Student will be able to understand
4
The 6 Classes of Fungi.
The differentiating characters among different Classes of fungi.
The importance of morphological characters in fungal differentiation and
classification.
5
Concept of ‘unculturable’
Strategies for culture of ‘unculturable’ bacteria.
Culture independent molecular methods for identifying unculturable bacteria.
Methods of extracting total bacterial DNA from a habitat and metagenome
analysis.
Resources
Recommended
Books
1. Jacquelyn G. Black (2013) Microbiology: Principles and
6th Edition, John Wiley & Sons, Inc.,
2. Microbial Diversity: Form and Function in
Online: 30 NOV 2007.
3. Ridley Mark (2004). Evolution. Blackwell Science Ltd.
4. Breed and Buchanan. Bergey’s Manual of Determinative
8th Edition, 1974.
5. Breed and Buchanan. Bergey’s Manual of
9th Edition, 1982.
6. Breed and Buchanan. Bergey’s Manual of Systematic
Edition, (Volumes. 1
Reference
Books
1. Sykes, G. and F. A. Skinner (Eds). Actinomycetales:
Practical
2. Bacteriology Symposium Series No. 2, Academic Press. 1973.
3. Jacquelyn G. Black (2013) Microbiology: Principles and
6th Edition, John Wiley & Sons, Inc.,
4. Lodder J. (1974). The Yeasts: A Taxonomic Study, North
Publishing Co. Amsterdam.
Student will be able to understand the concepts microbial taxonomy and methods of
Student will be able to use the knowledge for isolation and identification of
microorganisms from variety of sources.
Student will be able to classify the microorganisms based on classical and advanced
Student will be able to understand microbial diversity, species and estimation of various
microbial species from different sources.
Student will be able to understand evolution of microbial species.
The 6 Classes of Fungi.
The differentiating characters among different Classes of fungi.
The importance of morphological characters in fungal differentiation and
Concept of ‘unculturable’ bacterial diversity.
Strategies for culture of ‘unculturable’ bacteria.
Culture independent molecular methods for identifying unculturable bacteria.
Methods of extracting total bacterial DNA from a habitat and metagenome
Jacquelyn G. Black (2013) Microbiology: Principles and
6th Edition, John Wiley & Sons, Inc.,
Microbial Diversity: Form and Function in Prokaryotes,
Online: 30 NOV 2007. DOI: 10.1002/9780470750490.ch1
Ridley Mark (2004). Evolution. Blackwell Science Ltd.
Breed and Buchanan. Bergey’s Manual of Determinative
8th Edition, 1974.
Breed and Buchanan. Bergey’s Manual of Determinative
9th Edition, 1982.
Breed and Buchanan. Bergey’s Manual of Systematic
Edition, (Volumes. 1 – 5) (2001 – 2003).
Sykes, G. and F. A. Skinner (Eds). Actinomycetales: Characteristics and
Practical Importance. Society for Applied
Bacteriology Symposium Series No. 2, Academic Press. 1973.
Jacquelyn G. Black (2013) Microbiology: Principles and
6th Edition, John Wiley & Sons, Inc.,
Lodder J. (1974). The Yeasts: A Taxonomic Study, North
Publishing Co. Amsterdam.
Pa
ge2
microbial taxonomy and methods of
use the knowledge for isolation and identification of
classify the microorganisms based on classical and advanced
understand microbial diversity, species and estimation of various
The importance of morphological characters in fungal differentiation and
12 L
Culture independent molecular methods for identifying unculturable bacteria.
Methods of extracting total bacterial DNA from a habitat and metagenome
12 L
Total 60
Jacquelyn G. Black (2013) Microbiology: Principles and Explorations,
Prokaryotes, Published
DOI: 10.1002/9780470750490.ch1
Ridley Mark (2004). Evolution. Blackwell Science Ltd.
Breed and Buchanan. Bergey’s Manual of Determinative Bacteriology.
Determinative Bacteriology.
Breed and Buchanan. Bergey’s Manual of Systematic Bacteriology. 2nd
Characteristics and
Bacteriology Symposium Series No. 2, Academic Press. 1973.
Jacquelyn G. Black (2013) Microbiology: Principles and Explorations,
Lodder J. (1974). The Yeasts: A Taxonomic Study, North Holland
Year: First Year
Course: Instrumentation and Molecular Biophysics
Teaching
Scheme
(Hrs/Week)
Continuous Internal Assessment (CIA)
L T P C CIA-1 CIA-2
4 0 - 4 10 20
Max. Time, End Semester Exam (Theory)
Prerequisite • Introduction and basic concepts of
• Basic concept
Objectives
1 To create general understanding of
2 To familiarize the students with principles of bioinstrumentation used in qualitative and
quantitative analysis.
3 To develop the concepts and train the students to increase their competency
4 To develop analytical skills of students
5 To orient students towards biological research with the help of bioinstrumentation
techniques.
Unit
Number
1 Laboratory Instruments:
meter, Laminar air flow, Centrifuge machine types and Centrifugation:
Differential, Rate Zonal, Isopycnic, Density gradient, Rotor types and Ultra
centrifugation. Phase Contrast Microscope; Fluorescent Microscope; Scanning
and Transmission Electron Microscopy.
2 Chromatography Techniques
of Paper Chromatography,TLC, HPTLC, Gel Filtration Chromatography, Ion
Exchange Chromatography, Affinity Chromatography, Gas Chromatography,
and HPLC.
Electrophoretic Techniques
Electrophoresis, Poly Acrylamide Gel Electrophoresis (PAGE), Agarose Gel
Electrophoresis. Principle and Applications of: Iso
3
Radio-isotopic Techniques
Biological Applications, Radioactive Decay
Principles and Applications of GM (Geiger Muller) Counter, Solid and Liquid
Scintillation Counter, Autoradiography, Radioimmunoassay (RIA), Radiation
Dosimeters.
School of Science M.Sc. Microbiology
Semester: I
Instrumentation and Molecular Biophysics Course Code:
Continuous Internal Assessment (CIA) End Semester
Examination
2 CIA-3 CIA-4 Lab Theory Lab
10 10 - 50 -
End Semester Exam (Theory) - 3Hrs.
Introduction and basic concepts of instrumentation, analytical techniques
Basic concept related to biochemical and biophysical methods
understanding of various bioanalytical methods.
To familiarize the students with principles of bioinstrumentation used in qualitative and
To develop the concepts and train the students to increase their competency
develop analytical skills of students.
To orient students towards biological research with the help of bioinstrumentation
Details
Laboratory Instruments: Theory, Principle, Working and applications of: pH
Laminar air flow, Centrifuge machine types and Centrifugation:
Differential, Rate Zonal, Isopycnic, Density gradient, Rotor types and Ultra
centrifugation. Phase Contrast Microscope; Fluorescent Microscope; Scanning
and Transmission Electron Microscopy.
Chromatography Techniques: Theory, principle, operation and applications
of Paper Chromatography,TLC, HPTLC, Gel Filtration Chromatography, Ion
Exchange Chromatography, Affinity Chromatography, Gas Chromatography,
Electrophoretic Techniques: Theory, Principle and Applications of Paper
Electrophoresis, Poly Acrylamide Gel Electrophoresis (PAGE), Agarose Gel
Electrophoresis. Principle and Applications of: Iso-electric Focusing
isotopic Techniques : Introduction to Radioisotopes and th
Biological Applications, Radioactive Decay–Types and Measurement,
Principles and Applications of GM (Geiger Muller) Counter, Solid and Liquid
Scintillation Counter, Autoradiography, Radioimmunoassay (RIA), Radiation
Pa
ge1
Semester: I
Course Code: PMI102
Total
100
instrumentation, analytical techniques
related to biochemical and biophysical methods
To familiarize the students with principles of bioinstrumentation used in qualitative and
To develop the concepts and train the students to increase their competency.
To orient students towards biological research with the help of bioinstrumentation
Hours
Theory, Principle, Working and applications of: pH
Laminar air flow, Centrifuge machine types and Centrifugation:
Differential, Rate Zonal, Isopycnic, Density gradient, Rotor types and Ultra
centrifugation. Phase Contrast Microscope; Fluorescent Microscope; Scanning
1
: Theory, principle, operation and applications
of Paper Chromatography,TLC, HPTLC, Gel Filtration Chromatography, Ion
Exchange Chromatography, Affinity Chromatography, Gas Chromatography,
heory, Principle and Applications of Paper
Electrophoresis, Poly Acrylamide Gel Electrophoresis (PAGE), Agarose Gel
2
: Introduction to Radioisotopes and their
Types and Measurement,
Principles and Applications of GM (Geiger Muller) Counter, Solid and Liquid
Scintillation Counter, Autoradiography, Radioimmunoassay (RIA), Radiation
3
4 Molecular Biophysics:
of size of proteins, Physical nature of non
Conformational properties of proteins, Ramachandran plot, secondary, super
secondary, tertiary and quaternary structures of
dimensional structures of proteins (motifs and fold domains) Protein
structure/properties determination.
5 Spectroscopies of Biomolecules and Biophysical Techniques
1. UV/Visible spectroscopy
Lamberts Law, Bathochromic and hypsochromic shifts.
2. Fluorescence spectroscopy
FRET, Binding and Folding studies.
3. Infrared spectroscopy
and its advantages
4. Circular Dichroism (CD)
absorbance, Cotton Effect.
5. Mass spectroscopy: Principles of operation and types of spectrometers,
ionization, Ion fragmentation, Mass analyzers, GC
MALDI-TOF
6. X-ray crystallography: Isolation and purification of proteins, crystallization
of proteins, instrumentation, acquisition of the diffraction pattern, basic
principles of x-ray diffraction, Phase determination
7. NMR spectroscopy: Basic Principles of NMR, Chemical shift, Intensity, Line
width, Relaxation parameters, Spin
NMR Applications in Biology
Course Outcome
Students should able to
CO1 Student will be able to demonstrate an understanding
required in biological processes.
CO2 Student will be able to demonstrate the basic
bilogical studies.
CO3 Student will be able to demonstrate the basic idea and applications of
techniques
CO4 Student will be able to introduce
these concepts and ideas in technology
CO5 Student will be able to
approach.
Resources
Recommended
Books
• Clive Dennison (2002)
Publishers
• Pattabhi, V. and Gautham, N. (2002)
Publishers, New York and NarosaPublishingHouse, Delhi.
Biophysics:Theoretical and experimental methods for determination
of size of proteins, Physical nature of non-covalent interactions,
Conformational properties of proteins, Ramachandran plot, secondary, super
secondary, tertiary and quaternary structures of proteins, Classification of three
dimensional structures of proteins (motifs and fold domains) Protein
structure/properties determination.
Spectroscopies of Biomolecules and Biophysical Techniques
UV/Visible spectroscopy- Instrumentation, Molar Absorptivities, Beer and
Lamberts Law, Bathochromic and hypsochromic shifts.
2. Fluorescence spectroscopy- Instrumentation, Quantum Yield, Quenching,
FRET, Binding and Folding studies.
Infrared spectroscopy- Principle , Instrumentation, Absorption bands
4. Circular Dichroism (CD) – Instrumentation, Circular polarization, Delta
absorbance, Cotton Effect.
5. Mass spectroscopy: Principles of operation and types of spectrometers,
ionization, Ion fragmentation, Mass analyzers, GC-MS, Biological applications,
ray crystallography: Isolation and purification of proteins, crystallization
of proteins, instrumentation, acquisition of the diffraction pattern, basic
ray diffraction, Phase determination
spectroscopy: Basic Principles of NMR, Chemical shift, Intensity, Line
width, Relaxation parameters, Spin-spin coupling, Nuclear Overhauser Effect,
NMR Applications in Biology
Student will be able to demonstrate an understanding of various instruments that are
required in biological processes.
Student will be able to demonstrate the basic understanding of the instruments used for
to demonstrate the basic idea and applications of
Student will be able to introduce principle of basic techniques and train them to apply
these concepts and ideas in technology .
Student will be able touse the knowlegde of techniques and develop the problem
Clive Dennison (2002) A guide to protein isolation, KluwerAcademic
Pattabhi, V. and Gautham, N. (2002) Biophysics.
New York and NarosaPublishingHouse, Delhi.
Pa
ge2
Theoretical and experimental methods for determination
covalent interactions,
Conformational properties of proteins, Ramachandran plot, secondary, super-
proteins, Classification of three
dimensional structures of proteins (motifs and fold domains) Protein
4
Absorptivities, Beer and
Instrumentation, Quantum Yield, Quenching,
Principle , Instrumentation, Absorption bands, FTIR
Instrumentation, Circular polarization, Delta
5. Mass spectroscopy: Principles of operation and types of spectrometers,
Biological applications,
ray crystallography: Isolation and purification of proteins, crystallization
of proteins, instrumentation, acquisition of the diffraction pattern, basic
spectroscopy: Basic Principles of NMR, Chemical shift, Intensity, Line
spin coupling, Nuclear Overhauser Effect,
5
Total 60
of various instruments that are
of the instruments used for
to demonstrate the basic idea and applications of the instruments or
basic techniques and train them to apply
knowlegde of techniques and develop the problem-solving
, KluwerAcademic
Biophysics. KluwerAcademic
• David J Holme, Hazel Peck (1998)
Prentice Hall, Pearson Education Limited, HarlowEngland.
• Rodney F. Boyer (2000
edition,Benjamin Cummings.
• Nölting, B. (2006)
Germany.
• Wilson Keith and Walker John (2005)
Biochemistry and Molecular Biology,
New York.
• Pattabhi, V. and Gautha
Publishers, New York and NarosaPublishingHouse, Delhi.
• Rolf Ekman, Jerzy Silberring, Ann Westman
(2009) Mass spectrometry: instrumentation,interpretation, and applications
John Wiley & Sons
• Irwin H. Segel (1976)
SolveMathematical Problems in General Biochemistry,
Wiley & Sons.
• Nölting, B. (2006)
Germany.
• Pattabhi, V. an
Publishers, New York and NarosaPublishingHouse, Delhi.
• Cavanagh John
Practice, Academic Press.
• Keeler, J. (2002)
England.
• Drenth, J. (2007)
Springer, Germany.
• Nölting, B. (2006)
Springer,Germany.
• Cotterill, R. M. J. (2002)
England.
Reference Books
• David J Holme, Hazel Peck (1998)
Prentice Hall, Pearson Education Limited, HarlowEngland.
• Berg, J. M., Tymoczko, J. L. and Stryer, L. (2006)
Edition. Freeman, New York.
• Garrett, R. H. and Grisham, C. M. (2004)
Cole, Publishing Company, California
• Cotterill, R. M. J. (2002)
England.
• Christof M. Niemeyer and Chad A. Mirkin
Wiley & Sons.
• Daniel L. Feldheim and Colby A. Foss, Jr.
synthesis and characterization and applications
• Mahendra Rai and Nelson Duran (2011)
inMicrobiology
David J Holme, Hazel Peck (1998) Analytical Biochemistry
Prentice Hall, Pearson Education Limited, HarlowEngland.
Rodney F. Boyer (2000) Modern Experimental Biochemistry
edition,Benjamin Cummings.
Nölting, B. (2006) Methods in modern biophysics. SecondEdition. Springer,
Wilson Keith and Walker John (2005) Principles andTechniques of
Biochemistry and Molecular Biology, 6th Ed.Cambridge University Press,
Pattabhi, V. and Gautham, N. (2002) Biophysics.
Publishers, New York and NarosaPublishingHouse, Delhi.
Rolf Ekman, Jerzy Silberring, Ann Westman-Brinkmalm,AgnieszkaKraj
Mass spectrometry: instrumentation,interpretation, and applications
John Wiley & Sons,Inc.,Canada.
Irwin H. Segel (1976) Biochemical Calculations: How to
SolveMathematical Problems in General Biochemistry,
Wiley & Sons.
Nölting, B. (2006) Methods in modern biophysics. SecondEdition. Springer,
Pattabhi, V. and Gautham, N. (2002) Biophysics.
Publishers, New York and NarosaPublishingHouse, Delhi.
Cavanagh John et.al. (1995) Proteins NMR Spectroscopy:Principles and
, Academic Press.
Keeler, J. (2002) Understanding NMR Spectroscopy. JohnWiley& Sons,
Drenth, J. (2007) Principles of protein X-ray crystallography.
Springer, Germany.
Nölting, B. (2006) Methods in modern biophysics.
Springer,Germany.
Cotterill, R. M. J. (2002) Biophysics: An Introduction. JohnWiley& Sons,
David J Holme, Hazel Peck (1998) Analytical Biochemistry
Prentice Hall, Pearson Education Limited, HarlowEngland.
Berg, J. M., Tymoczko, J. L. and Stryer, L. (2006)
Freeman, New York.
Garrett, R. H. and Grisham, C. M. (2004) Biochemistry.
Cole, Publishing Company, California
Cotterill, R. M. J. (2002) Biophysics: An Introduction. JohnWiley& Sons,
Christof M. Niemeyer and Chad A. Mirkin (2006)Nanobiotechnology
Wiley & Sons.
Daniel L. Feldheim and Colby A. Foss, Jr. (2002) Metalnanoparticles
synthesis and characterization and applicationsMarcel Dekker, Inc.
Mahendra Rai and Nelson Duran (2011) Metal nanoparticles
inMicrobiology, Springer Verlag Berlin Heidelberg.
Pa
ge3
Analytical Biochemistry,3rded .,
) Modern Experimental Biochemistry3d
SecondEdition. Springer,
Principles andTechniques of
6th Ed.Cambridge University Press,
Biophysics. KluwerAcademic
Brinkmalm,AgnieszkaKraj
Mass spectrometry: instrumentation,interpretation, and applications,
Biochemical Calculations: How to
SolveMathematical Problems in General Biochemistry, 2nd Edition.John
SecondEdition. Springer,
Biophysics. KluwerAcademic
Proteins NMR Spectroscopy:Principles and
JohnWiley& Sons,
ray crystallography.3rd Ed.
Methods in modern biophysics. SecondEdition.
JohnWiley& Sons,
Analytical Biochemistry,3rd Ed.,
Berg, J. M., Tymoczko, J. L. and Stryer, L. (2006)Biochemistry. 6th
Biochemistry. 3rd
Ed. Brooks/
JohnWiley& Sons,
Nanobiotechnology, John
(2002) Metalnanoparticles
Marcel Dekker, Inc.
Metal nanoparticles
Year: First Year
Course: Cell and Molecular Biology
Teaching
Scheme
(Hrs/Week)
Continuous Internal Assessment (CIA)
L T P C CIA-1 CIA-2
4 0 - 4 10 20
Max. Time, End Semester Exam (Theory)
Prerequisite • Introduction and basic concepts of
Objectives
1 To introduce the fundamental
molecular level.
2 To familiarize the students with the importance of cellular and molecular process for
encouragement towards research and develop scientific thinking
3 To develop proficiency and technical skills of students.
4 To apply the knowledge
various problems.
5 To develop research driven approach for better understanding of
biological processes.
Unit
Number
1
Structural organization Membrane and Communication of cell
1.1- Introduction, History and General organization of Prokaryotic cell
Mycoplasma, Viruses, Viroids and Bacteria (
1.2 General organization of Eukaryotic cell (Plant cell)
1.3 Composition of cell membrane
1.4 Molecular modelsmosaic Model.
1.5 Functions of cell membrane
1.6General principles of cell signaling.
1.7 G-protein linked receptors.
1.8 Enzyme linked receptors.
1.9 Mitosis and Meiosis
Structural organization, functions of cell organelles and Cytoskeleton
2.1 Nucleus-Ultrastructure, Nuclear envelop, Pore complex
School of Science
M.Sc. Microbiology
Semester: I
Cell and Molecular Biology Course Code:
Continuous Internal Assessment (CIA) End Semester
Examination
2 CIA-3 CIA-4 Lab Theory Lab
10 10 - 50 -
End Semester Exam (Theory) - 3Hrs.
Introduction and basic concepts of living cell, cellular system etc
fundamental concepts and processes of living organisms at cellular
To familiarize the students with the importance of cellular and molecular process for
encouragement towards research and develop scientific thinking.
To develop proficiency and technical skills of students.
pply the knowledge cellular and molecular mechanism of biological system
develop research driven approach for better understanding of
Details
Structural organization Membrane and Communication of cell
Introduction, History and General organization of Prokaryotic cell
Mycoplasma, Viruses, Viroids and Bacteria (E.coli).
1.2 General organization of Eukaryotic cell (Plant cell)
1.3 Composition of cell membrane
Molecular models-Unit membrane, Daniely and Dowson͛s Model
1.5 Functions of cell membrane
1.6General principles of cell signaling.
protein linked receptors.
1.8 Enzyme linked receptors.
1.9 Mitosis and Meiosis
Structural organization, functions of cell organelles and Cytoskeleton
Ultrastructure, Nuclear envelop, Pore complex Pa
ge1
Semester: I
Course Code: PMI103
Total
100
living cell, cellular system etc.
and processes of living organisms at cellular and
To familiarize the students with the importance of cellular and molecular process for
cellular and molecular mechanism of biological system to address
develop research driven approach for better understanding of the applications of
Hours
Introduction, History and General organization of Prokaryotic cell-
Daniely and Dowson͛s Model, Fluid
12 L
Structural organization, functions of cell organelles and Cytoskeleton 12 L
2 2.2 Mitochondria-Morphology, molecular structure, membranes, molecular
organization, Biogene
Genetic code.
2.3 Chloroplast-Morphology, Ultra structure, molecular organization of
thylakoids, Biogenesis, DNA, Semi
2.4 Golgi complex- Morphology, cytochemistry
2.5 Endoplasmic reticulum
3
Structural organization, functions of cell organelles and:
Cytoskeleton: 3.1Ribosomes
3.2Structure and functions of Lysosomes, Vacuoles, Peroxisomes.
3.3Microtubules-cillia, flagella, & centrioles.
3.4Intermediate filaments.
3.5Microfilaments and cell motility.
4
Deoxyribose Nucleic Acid
4.1 DNA-chemical composition, bases, sugar, phosphate, Nucleosides,
Nucleotides, Polynucleotides, Bonding systems, Watson and Crick double
helix model of DNA, Types
4.2 DNA Replication
Origin of replication (OriC in
synthesis, Leading strand, Lagging strand, Okazaki fragments, prokaryotic
and Eukaryotic enzymes involved in DNA
Eukaryotic enzymes involved in DNA
bidirectional DNA- replication,
4.3-Replication in closed circular DNA
replication.
4.4-DNA-damages and Repair:
4.6 Significance of DNA
5
Ribose Nucleic acid, Gene Regulation and Transposons
5.1-Structure and functions of tRNA, rRNA, mRNA
5.2-Transcription-Capping, elongation, and termination, Role of RNA
polymerases.
5.3-Processing of RNA
5.4 Constitutive and regulated genes, Positive and negative con
transcriptional control, translational control, and post
5.5 Gene regulation in Prokaryotes
operon-trp operon.
Course Outcome
Students should able to
CO1 Student will be able to d
CO2 Student will be able to e
Morphology, molecular structure, membranes, molecular
organization, Biogenesis, DNA, Symbiotic hypothesis Termination codon,
Morphology, Ultra structure, molecular organization of
thylakoids, Biogenesis, DNA, Semi-autonomous nature.
Morphology, cytochemistry
reticulum-Morphology, Biogenesis, protein segregation
Structural organization, functions of cell organelles and:
3.1Ribosomes-types, structure.
ctions of Lysosomes, Vacuoles, Peroxisomes.
a, flagella, & centrioles.
3.4Intermediate filaments.
3.5Microfilaments and cell motility.
Deoxyribose Nucleic Acid
chemical composition, bases, sugar, phosphate, Nucleosides,
Nucleotides, Polynucleotides, Bonding systems, Watson and Crick double
helix model of DNA, Types-A, B, and Z DNA.
4.2 DNA Replication-Replicon, Replisome, Primosome, Replication fork,
Origin of replication (OriC in E. coli) RNA-primer, Semidiscontinuos
nthesis, Leading strand, Lagging strand, Okazaki fragments, prokaryotic
and Eukaryotic enzymes involved in DNA- fragments, prokaryotic and
Eukaryotic enzymes involved in DNA- replication, Unidirectional and
replication,
in closed circular DNA- θ-mode, σ-mode and D
damages and Repair:-Damages-Dimer formations, mismatch pair,
4.6 Significance of DNA.
Ribose Nucleic acid, Gene Regulation and Transposons
Structure and functions of tRNA, rRNA, mRNA
Capping, elongation, and termination, Role of RNA
Processing of RNA- RNA-editing, splicing, polyadenylation
5.4 Constitutive and regulated genes, Positive and negative con
transcriptional control, translational control, and post-translational control
5.5 Gene regulation in Prokaryotes- Inducible operon-lac operon, Represible
be able to describe fundamental processes of cell.
Student will be able to explain the cellular processes of biological system
Pa
ge2
Morphology, molecular structure, membranes, molecular
sis, DNA, Symbiotic hypothesis Termination codon,
Morphology, Ultra structure, molecular organization of
Morphology, Biogenesis, protein segregation.
12 L
chemical composition, bases, sugar, phosphate, Nucleosides,
Nucleotides, Polynucleotides, Bonding systems, Watson and Crick double
Replicon, Replisome, Primosome, Replication fork,
primer, Semidiscontinuos
nthesis, Leading strand, Lagging strand, Okazaki fragments, prokaryotic
fragments, prokaryotic and
replication, Unidirectional and
mode and D-loop
Dimer formations, mismatch pair,
12 L
Capping, elongation, and termination, Role of RNA-
5.4 Constitutive and regulated genes, Positive and negative control,
translational control
lac operon, Represible
12 L
Total 60
cellular processes of biological system
CO3 Student will be able to
system.
CO4 Student will be able to
sciences
CO5 Student will be able to
of biological challenges
Resources
Recommended
Books
1. Strickberger. Genetics. 3rd Ed. 1990.
2. A. K. Sharma and A. Sharma. 1990. Chromosome techniques
3. P.K. Gupta. 1990. Genetics.
4. Principles & Methods in Plant Molecular
Biology,Genetics&Biochemistry,Agrobios. Prathibha Devi
5. A. K. Sharma and A. Sharma. 1990. Chromosome techniques.
Reference Books
1. E.D.P. De Robertis and E. M. F. De Robertis. 1987. Cell and Molecular
biology8th Ed(Indian Ed
2. G. M. Cooper. 1997. The Cell and Molecular approach. ASM Press. Ed.
3. Snustad and Simmons. 1997. Principles of Genetics. Ed.
4.Benjamin Lew
5. Daniel Hartl. 1994. Basic Genetics. Ed
6.Winter, Hicky and Fletcher. 1999. Instant notes in Genetics. Ed.
7.A.V.S.S. Sambamurthy. 1999. Genetics.
8.P.K. Gupta. 1990. Genetics.
9. Twyman. 1998. Advanced Molecular Biology.
10.Turner,
MolecularBiology.
11.Primrose. 1999. Molecular Biotechnology.
12.Stansfield. 1996. Theory & Problems in Genetics. Schaum’s Series.
McGraw & Hill.
Student will be able to understand the molecular mechanism involved in
Student will be able to apply the knowledge to solve problems related to biological
Student will be able to understand gene regulation mechanism to address number
of biological challenges
1. Strickberger. Genetics. 3rd Ed. 1990. Ed.
A. K. Sharma and A. Sharma. 1990. Chromosome techniques
P.K. Gupta. 1990. Genetics.
Principles & Methods in Plant Molecular
Biology,Genetics&Biochemistry,Agrobios. Prathibha Devi
5. A. K. Sharma and A. Sharma. 1990. Chromosome techniques.
E.D.P. De Robertis and E. M. F. De Robertis. 1987. Cell and Molecular
biology8th Ed(Indian Ed
2. G. M. Cooper. 1997. The Cell and Molecular approach. ASM Press. Ed.
3. Snustad and Simmons. 1997. Principles of Genetics. Ed.
4.Benjamin Lewis. 1999. Genes VII.
5. Daniel Hartl. 1994. Basic Genetics. Ed
6.Winter, Hicky and Fletcher. 1999. Instant notes in Genetics. Ed.
7.A.V.S.S. Sambamurthy. 1999. Genetics.
8.P.K. Gupta. 1990. Genetics.
9. Twyman. 1998. Advanced Molecular Biology.
10.Turner, Mclennon, Bates and White. 1999. Instant notes in
MolecularBiology.
11.Primrose. 1999. Molecular Biotechnology.
12.Stansfield. 1996. Theory & Problems in Genetics. Schaum’s Series.
McGraw & Hill.
Pa
ge3
understand the molecular mechanism involved in biological
related to biological
understand gene regulation mechanism to address number
A. K. Sharma and A. Sharma. 1990. Chromosome techniques
Principles & Methods in Plant Molecular
5. A. K. Sharma and A. Sharma. 1990. Chromosome techniques.
E.D.P. De Robertis and E. M. F. De Robertis. 1987. Cell and Molecular
2. G. M. Cooper. 1997. The Cell and Molecular approach. ASM Press. Ed.
6.Winter, Hicky and Fletcher. 1999. Instant notes in Genetics. Ed.
Mclennon, Bates and White. 1999. Instant notes in
12.Stansfield. 1996. Theory & Problems in Genetics. Schaum’s Series.
Year: First Year
Course: Enzymes and Microbial Metabolism
Teaching
Scheme
(Hrs/Week)
Continuous Internal Assessment (CIA)
L T P C CIA-1 CIA-2
4 0 - 4 10 20
Max. Time, End Semester Exam (Theory)
Prerequisite 1. Introduction and basic concepts of
2. Basic concept
Objectives
1 To impart the knowledge of microbial physiology and biochemistry that
develop the concepts and skills related to subject.
2 To understand and critically evaluate research that addresses physiological and
biochemical processes of microbes.
3 To encourage application of the theories related to microbial metabolism
bio-products and to address current problems.
4 To impart the knowledge of
5 To develop an understanding of metabolic processes carried out in all kind of living
systems
Unit
Number
1 Enzyme Kinetics
Purifications of enzyme, purification chart, kinetics of single substrate
enzyme catalyzed reaction.
Kinetics of reversible inhibitions enzyme catalyzed reactions, King Altman
approach to derive –
substrate enzyme catalyzed reactions, concept of allosterism, positive and
negative co-operatively, models of allosteric enzymes (Monod, Wyamann
and Changuaxmodel,Koshland, Nemethy and Filmer model), kinetics of
allosteric
enzyme, Hill plot, examples of allosteric enzymes and their significance in
allosteric regulation
2 Bio-energetic
Laws of thermodynamics, entropy, enthalpy, free energy, freeenergy and
equilibrium constant, Gibbs free energy equation,determination o
School of Science
M.Sc. Microbiology
Semester: I
Microbial Metabolism Course Code:
Continuous Internal Assessment (CIA) End Semester
Examination
2 CIA-3 CIA-4 Lab Theory Lab
10 10 - 50 -
End Semester Exam (Theory) - 3Hrs.
Introduction and basic concepts of enzymes
Basic concept of microbial physiology
To impart the knowledge of microbial physiology and biochemistry that
develop the concepts and skills related to subject.
To understand and critically evaluate research that addresses physiological and
biochemical processes of microbes.
To encourage application of the theories related to microbial metabolism
products and to address current problems.
To impart the knowledge of enzymes, its kinetics, extraction and applications.
develop an understanding of metabolic processes carried out in all kind of living
Details
Purifications of enzyme, purification chart, kinetics of single substrate
enzyme catalyzed reaction.
Kinetics of reversible inhibitions enzyme catalyzed reactions, King Altman
two substrate enzyme catalyzed reactions, types of two
substrate enzyme catalyzed reactions, concept of allosterism, positive and
operatively, models of allosteric enzymes (Monod, Wyamann
and Changuaxmodel,Koshland, Nemethy and Filmer model), kinetics of
enzyme, Hill plot, examples of allosteric enzymes and their significance in
Laws of thermodynamics, entropy, enthalpy, free energy, freeenergy and
equilibrium constant, Gibbs free energy equation,determination o
Semester: I
Course Code: PMI104
Total
100
To impart the knowledge of microbial physiology and biochemistry that needed to
To understand and critically evaluate research that addresses physiological and
To encourage application of the theories related to microbial metabolism for production of
enzymes, its kinetics, extraction and applications.
develop an understanding of metabolic processes carried out in all kind of living
Hours
Purifications of enzyme, purification chart, kinetics of single substrate
Kinetics of reversible inhibitions enzyme catalyzed reactions, King Altman
catalyzed reactions, types of two
substrate enzyme catalyzed reactions, concept of allosterism, positive and
operatively, models of allosteric enzymes (Monod, Wyamann
and Changuaxmodel,Koshland, Nemethy and Filmer model), kinetics of
enzyme, Hill plot, examples of allosteric enzymes and their significance in
12 L
Laws of thermodynamics, entropy, enthalpy, free energy, freeenergy and
equilibrium constant, Gibbs free energy equation,determination of free
12 L
energy of hydrolytic and biological oxidation reduction reactions, under
standard and non-standardconditions, high energy compounds, coupled
reactions,determination of feasibility of reactions, Atkinson’s energy charge,
phosphorylation potential and
3 Membrane Transport
Aerobic and anaerobic respirationmitochondrial electron transport chain,
structure and function of ATPase, generation and maintenance of proton
motive force,oxidative phosphorylation, inhibitors and un
electrontransport chain and oxidative phosphorylation.
Concept of anaerobic respiration, components of electrontransfer system and
energy generation of bacteria where nitrate,sulfate and carbonate acts as
terminal electron acceptors
4 Nitrogen metabolism
Biochemistry of biological nitrogen fixation, properties of nitrogenase and
its regulation, ammonia assimilation with respect to glutamine synthetase,
glutamate dehydrogenase, glutamate synthetase, their properties and
regulation,Biosynthesis of
Biosynthesis of purine and pyrimidine bases
Photosynthesis
Structure of chloroplast, energy consideration in photosynthesis, light and
dark reaction, electron carriers in photosynthesis, Organization of
photosystem I and II, cyclic and non
reaction, photolysis of water, C
Regulation of photosynthesis, Bacterial photosynthesis: scope, electron
carriers, Photosynthetic reaction center, cycl
photophosphorylation in various groups of phototrophic bacteria, electron
donors other than water in anoxygenic photosynthetic bacteria
5 Biosynthesis of carbohydrates in plants and bacteria
Calvin cycle and its
membrane, Synthesis of starch and sucrose,Photorespiration, C
pathways, synthesis of celluloseand peptidoglycan, integration of
carbohydrate metabolism inplant cell.
Lipid Biosynthesis
Synthesis of storage lipids: Fatty acids and triacylglycerols,Synthesis of
membrane lipids: Glycerophospholipids, sphingolipids, sterols, Lipids as
signal molecules such asphosphatidyl inositol, eicosanoids, Vitamins, A, D,
K, and E,Dolichols.
Course Outcome
Students should able to
CO1 Student will be able to understand the concepts of microbial physiology
CO2 Student will be able to understand the metabolic processes carried out in microorganisms
energy of hydrolytic and biological oxidation reduction reactions, under
standardconditions, high energy compounds, coupled
reactions,determination of feasibility of reactions, Atkinson’s energy charge,
phosphorylation potential and its significance
Membrane Transport
Aerobic and anaerobic respirationmitochondrial electron transport chain,
structure and function of ATPase, generation and maintenance of proton
motive force,oxidative phosphorylation, inhibitors and un-couplers
electrontransport chain and oxidative phosphorylation.
Concept of anaerobic respiration, components of electrontransfer system and
energy generation of bacteria where nitrate,sulfate and carbonate acts as
terminal electron acceptors
metabolism
Biochemistry of biological nitrogen fixation, properties of nitrogenase and
its regulation, ammonia assimilation with respect to glutamine synthetase,
glutamate dehydrogenase, glutamate synthetase, their properties and
regulation,Biosynthesis of five families of amino acids and histidine,
Biosynthesis of purine and pyrimidine bases
Structure of chloroplast, energy consideration in photosynthesis, light and
dark reaction, electron carriers in photosynthesis, Organization of
tem I and II, cyclic and non-cyclic flow of electrons, Z scheme, Hill
reaction, photolysis of water, C3, C4 CAM plants, Photorespiration,
Regulation of photosynthesis, Bacterial photosynthesis: scope, electron
carriers, Photosynthetic reaction center, cyclic flow of electrons, bacterial
photophosphorylation in various groups of phototrophic bacteria, electron
donors other than water in anoxygenic photosynthetic bacteria
Biosynthesis of carbohydrates in plants and bacteria
Calvin cycle and its regulation, Transport of solute acrosschloroplast
membrane, Synthesis of starch and sucrose,Photorespiration, C4 and CAM
pathways, synthesis of celluloseand peptidoglycan, integration of
carbohydrate metabolism inplant cell.
storage lipids: Fatty acids and triacylglycerols,Synthesis of
membrane lipids: Glycerophospholipids, sphingolipids, sterols, Lipids as
signal molecules such asphosphatidyl inositol, eicosanoids, Vitamins, A, D,
understand the concepts of microbial physiology
understand the metabolic processes carried out in microorganisms
energy of hydrolytic and biological oxidation reduction reactions, under
standardconditions, high energy compounds, coupled
reactions,determination of feasibility of reactions, Atkinson’s energy charge,
Aerobic and anaerobic respirationmitochondrial electron transport chain,
structure and function of ATPase, generation and maintenance of proton
couplers of
Concept of anaerobic respiration, components of electrontransfer system and
energy generation of bacteria where nitrate,sulfate and carbonate acts as
12 L
Biochemistry of biological nitrogen fixation, properties of nitrogenase and
its regulation, ammonia assimilation with respect to glutamine synthetase,
glutamate dehydrogenase, glutamate synthetase, their properties and
five families of amino acids and histidine,
Structure of chloroplast, energy consideration in photosynthesis, light and
dark reaction, electron carriers in photosynthesis, Organization of
cyclic flow of electrons, Z scheme, Hill
CAM plants, Photorespiration,
Regulation of photosynthesis, Bacterial photosynthesis: scope, electron
ic flow of electrons, bacterial
photophosphorylation in various groups of phototrophic bacteria, electron
12 L
regulation, Transport of solute acrosschloroplast
and CAM
pathways, synthesis of celluloseand peptidoglycan, integration of
storage lipids: Fatty acids and triacylglycerols,Synthesis of
membrane lipids: Glycerophospholipids, sphingolipids, sterols, Lipids as
signal molecules such asphosphatidyl inositol, eicosanoids, Vitamins, A, D,
12 L
Total 60
understand the metabolic processes carried out in microorganisms
CO3 Student will be able to
microbial metabolism
CO4 Student will be able to
enzymes from biological systems
CO5 Student will be able to integrate
Resources
Recommended
Books
1. Cox M. M., Nelson D. L., (2008) Lehninger Principles ofBiochemistry,
Fifth edition, W. H. Frreman and CompanyNew York
2. Garrett, R. H. and Grisham, C. M. (2004)
Brooks/Cole, Publishing Company, California.
3. Hall D. D. and Rao K. K. (1996)
University Press
of Bacterial Growth
4. Michael T. Madigan, John M. Martinko, David A. Stahl,David P. Clark
(2012) Brock Biology of Microorganisms
Cummings, San Francisco.
5. Moat Albert G. and Foster John W. (1988)
John Wileyand Sons New York.
Reference Books
6. Nelson D. L. and Cox M. M. (2005)
Fourth edition, W. H. Freeman & Co. NewYork.
7. Palmer Trevor (2001)
chemistry, Horwood Pub.
8. White David (2000)
Oxford University Press, New York.
Student will be able to understand the role of enzymes in biological systems including
Student will be able to study enzyme kinetics, extraction and purification methods of
enzymes from biological systems
to integrate the fundamental processes with their applications
Cox M. M., Nelson D. L., (2008) Lehninger Principles ofBiochemistry,
Fifth edition, W. H. Frreman and CompanyNew York
Garrett, R. H. and Grisham, C. M. (2004) Biochemistry.
Brooks/Cole, Publishing Company, California.
Hall D. D. and Rao K. K. (1996) Photosynthesis 5th Ed.,Cambridge
University PressMandelstam Joel and McQuillen Kenneth (1976)
of Bacterial Growth, Blackwell ScientificPublication London.
Michael T. Madigan, John M. Martinko, David A. Stahl,David P. Clark
Brock Biology of Microorganisms,Thirteenth edition, Benjamin
Cummings, San Francisco.
Moat Albert G. and Foster John W. (1988) MicrobialPhysiology
yand Sons New York.
Nelson D. L. and Cox M. M. (2005) Lehninger’s Principles ofBiochemistry
Fourth edition, W. H. Freeman & Co. NewYork.
Palmer Trevor (2001) Enzymes: Biochemistry, Biotechnologyand Clinical
, Horwood Pub. Co. Chinchester,England.
White David (2000) Physiology and Biochemistry ofProkaryotes.
Oxford University Press, New York.
understand the role of enzymes in biological systems including
study enzyme kinetics, extraction and purification methods of
the fundamental processes with their applications
Cox M. M., Nelson D. L., (2008) Lehninger Principles ofBiochemistry,
Biochemistry. 3rd
Ed.
5th Ed.,Cambridge
Mandelstam Joel and McQuillen Kenneth (1976)Biochemistry
ion London.
Michael T. Madigan, John M. Martinko, David A. Stahl,David P. Clark
,Thirteenth edition, Benjamin
MicrobialPhysiology2nd Ed.
Lehninger’s Principles ofBiochemistry,
Enzymes: Biochemistry, Biotechnologyand Clinical
Physiology and Biochemistry ofProkaryotes. 2nd Ed.
Year: First Year
Course: Microbial Diversity and Taxonomy A
and Molecular Biophysics Laboratory
Teaching
Scheme
(Hrs/Week)
Continuous Internal Assessment (CIA)
L T P C CIA-1 CIA-2
0 0 4 2 - -
Max. Time, End Semester Exam (Theory)
Objectives
1 To acquire proficiency in identification of mi
2 To train the students in advance microbial techniques.
3 To impart the knowledge of analytical methods in biological sciences
Sr.
No.
1 Isolation of the following types of bacteria from natural sampl
Identification of the bacteria to at least the Genus level using the Bergey’s Manuals:
The identification key must be designed for each isolated and identified bacterium. 2 Isolation of the following types of fungi from natural samples.
Identification of the fungi. Molds (Saprophytic); Yeasts
The identification key must be designed for each isolated and identified fungus. 3 Isolation and identification of any one type of cyanobacterium from a natural sample.
The identification key must be designed4 Preparation of buffers.
5 Chromatography: separation of sugar and amino acids by paper and thin layer
chromatography. 6 Colorimetry and spectrophotometry: Estimation of sugar and total carbohydrates,
estimation of protein by lowry. Bradford and UV spectrophotometry. 7 Electrophoresis: Agarose gel electrophoresis,
8 Electrophoresis: SDS PAGE of proteins.
Term Work:
Term Work assessment shall be conducted for the Project, Tutorials and Seminar. Ter
continuous assessment based on
journal/record book, oral/viva, respectively
At the end of the semester, the final grade for
performance of the student and is to be submitted to the University.
School of Science
M.Sc. Microbiology
Semester: I
ial Diversity and Taxonomy And Instrumentation
Laboratory
Course Code:
Continuous Internal Assessment (CIA) End Semester
Examination
2 CIA-3 CIA-4 Lab Theory Lab
- - 50 - 50
End Semester Exam (Theory) - 3Hrs.
To acquire proficiency in identification of microorganisms using Bergey’s manual
To train the students in advance microbial techniques.
To impart the knowledge of analytical methods in biological sciences.
Description
Isolation of the following types of bacteria from natural samples.
Identification of the bacteria to at least the Genus level using the Bergey’s Manuals:
The identification key must be designed for each isolated and identified bacterium.
Isolation of the following types of fungi from natural samples.
ion of the fungi. Molds (Saprophytic); Yeasts
The identification key must be designed for each isolated and identified fungus.
Isolation and identification of any one type of cyanobacterium from a natural sample.
The identification key must be designed for each isolated and identified cyanobacterium.
Chromatography: separation of sugar and amino acids by paper and thin layer
Colorimetry and spectrophotometry: Estimation of sugar and total carbohydrates,
estimation of protein by lowry. Bradford and UV spectrophotometry.
Electrophoresis: Agarose gel electrophoresis,
Electrophoresis: SDS PAGE of proteins.
Term Work assessment shall be conducted for the Project, Tutorials and Seminar. Ter
continuous assessment based on attendance, good laboratory practice (GPL), timely completion,
journal/record book, oral/viva, respectively. It should be assessed by course teacher of the institute.
At the end of the semester, the final grade for a Term Work shall be assigned based on the
performance of the student and is to be submitted to the University.
Semester: I
Course Code: PMI111
Total
100
croorganisms using Bergey’s manual.
Identification of the bacteria to at least the Genus level using the Bergey’s Manuals:
The identification key must be designed for each isolated and identified bacterium.
The identification key must be designed for each isolated and identified fungus.
Isolation and identification of any one type of cyanobacterium from a natural sample.
for each isolated and identified cyanobacterium.
Chromatography: separation of sugar and amino acids by paper and thin layer
Colorimetry and spectrophotometry: Estimation of sugar and total carbohydrates,
Term Work assessment shall be conducted for the Project, Tutorials and Seminar. Term work is
attendance, good laboratory practice (GPL), timely completion,
teacher of the institute.
a Term Work shall be assigned based on the
Notes
1 The regular attendance of the
marks will be given accordingly (
2 Good Laboratory Practices (
3 Timely Completion (10 Marks)
4 Journal / Record Book (10 Marks)
5 Oral / Viva (10 Marks)
Practical/Oral/Presentation:
Practical/Oral/Presentation shall be conducted and assessed jointly by at least
appointed as internal and external
mark/grade sheet in the format as specified by the University, authenticate and seal it. Sealed envelope
shall be submitted to the head of th
Notes
1 One experiment from the regular practical syllabus will be conducted (40 Marks).
2 Oral/Viva-voce (10 Marks).
the students during semester for practical course will be monitored and
rks will be given accordingly (10 Marks).
Good Laboratory Practices (10 Marks)
Timely Completion (10 Marks)
Journal / Record Book (10 Marks)
Practical/Oral/Presentation shall be conducted and assessed jointly by at least
internal and external examiners by the University. The examiners will prepare the
mark/grade sheet in the format as specified by the University, authenticate and seal it. Sealed envelope
shall be submitted to the head of the department or authorized person.
One experiment from the regular practical syllabus will be conducted (40 Marks).
practical course will be monitored and
Practical/Oral/Presentation shall be conducted and assessed jointly by at least a pair of examiners
The examiners will prepare the
mark/grade sheet in the format as specified by the University, authenticate and seal it. Sealed envelope
One experiment from the regular practical syllabus will be conducted (40 Marks).
Year: First Year
Course: Cell and Molecular Biology and Enzyme & Microbial
Metabolism Laboratory
Teaching
Scheme
(Hrs/Week)
Continuous Internal Assessment (CIA)
L T P C CIA-1 CIA-2
0 0 4 2 - -
Max. Time, End Semester Exam (Theory)
Objectives
1 To understand various cell activities using microscopic techniques.
2 To create the general understanding among students related to production of bio
3 To give the overview for development of scientific methods of screening, characterizing
microbes and their use in production of microbial products.
Sr. No.
1 Studying the stages mitosis in growing tip of onion root cells
2 Demonstration of mounting of embryo
stages on permanent slides
3 Isolation and characterization of bacterial pigment
4 Isolation and estimation of chromosomal DNA of bacteria
5 Isolation and characterization of (as nitrogen fixers) Rhizob
6 Detection of siderophore production by
7 Production and extraction of bacterial enzyme. Purification of enzyme by ammonium
sulphate precipitation and dialysis.
8 Isolation and characterization of biodeg
Term Work:
Term Work assessment shall be conducted for the Project, Tutorials and Seminar. Term work is
continuous assessment based on Attendance, G
Journal/Record book and Oral. It
the semester, the final grade for a Term Work shall be assigned based on the performance of the
student and is to be submitted to the University.
Notes
1 The regular attendance of the
marks will be given accordingly (10
School of Science
M.Sc. Microbiology
Semester: I
and Molecular Biology and Enzyme & Microbial Course Code:
Continuous Internal Assessment (CIA) End Semester
Examination
2 CIA-3 CIA-4 Lab Theory Lab
- - 50 - 50
End Semester Exam (Theory) - 3Hrs.
To understand various cell activities using microscopic techniques.
To create the general understanding among students related to production of bio
for development of scientific methods of screening, characterizing
microbes and their use in production of microbial products.
Description
Studying the stages mitosis in growing tip of onion root cells.
Demonstration of mounting of embryos (frog and fruit fly) at various developmental
stages on permanent slides.
Isolation and characterization of bacterial pigment.
Isolation and estimation of chromosomal DNA of bacteria.
Isolation and characterization of (as nitrogen fixers) Rhizobium or
Detection of siderophore production by Azospirillum and Pseudomonas
Production and extraction of bacterial enzyme. Purification of enzyme by ammonium
sulphate precipitation and dialysis.
Isolation and characterization of biodegradable bacteria.
Term Work assessment shall be conducted for the Project, Tutorials and Seminar. Term work is
based on Attendance, Good Laboratory Practice (GLP)
Journal/Record book and Oral. It should be assessed by subject teacher of the institute. At the end of
the semester, the final grade for a Term Work shall be assigned based on the performance of the
student and is to be submitted to the University.
the students during semester for practical course will be monitored and
rks will be given accordingly (10 Marks).
Semester: I
Course Code: PMI112
Total
100
To create the general understanding among students related to production of bio-products.
for development of scientific methods of screening, characterizing
s (frog and fruit fly) at various developmental
ium or Azospirillum
Pseudomonas
Production and extraction of bacterial enzyme. Purification of enzyme by ammonium
Term Work assessment shall be conducted for the Project, Tutorials and Seminar. Term work is
(GLP), Timely Completion,
d be assessed by subject teacher of the institute. At the end of
the semester, the final grade for a Term Work shall be assigned based on the performance of the
practical course will be monitored and
2 Good Laboratory Practices (
3 Timely Completion (10 Marks)
4 Journal / Record Book (10 Marks)
5 Oral / Viva (10 Marks)
Practical/Oral/Presentation:
Practical/Oral/Presentation shall be conducted and assessed jointly by at least a pair of examiners
appointed as internal and external
mark/grade sheet in the format as specified by the University, authenticate and seal it. Sealed envelope
shall be submitted to the head of the department or authorized person.
Notes
1 One experiment from the regular practical syllabus will be conducted (40 Marks).
2 Oral/Viva-voce (10 Marks).
Good Laboratory Practices (10 Marks)
Timely Completion (10 Marks)
Journal / Record Book (10 Marks)
Practical/Oral/Presentation shall be conducted and assessed jointly by at least a pair of examiners
internal and external examiners by the University. The examiners will prepare the
as specified by the University, authenticate and seal it. Sealed envelope
shall be submitted to the head of the department or authorized person.
One experiment from the regular practical syllabus will be conducted (40 Marks).
Practical/Oral/Presentation shall be conducted and assessed jointly by at least a pair of examiners
The examiners will prepare the
as specified by the University, authenticate and seal it. Sealed envelope
One experiment from the regular practical syllabus will be conducted (40 Marks).