(WSD)

Litopenaeus vannamei

(WSD)Litopenaeus vannamei

MINISTRY OF JIHAD - E - AGRICULTURE

AGRICULTURAL RESEARCH, EDUCATION & EXTENTION ORGANIZATION

IRANIAN FISHERIES RESEARCH ORGANIZATION- Iran Shrimp Research Center

Title:

Investigation on Effects of ergosan and vibromax

in control of White spot disease(WSD) and Vibriosis in

shrimp Litopenaeus vannamei

Executor :

Aghil Dashtian Nasab

Registration Number

2009.1214

Ministry of Jihad e Agriculture AGRICULTURAL RESEARCH, EDUCATION & EXTENTION ORGANIZATION IRANIAN FISHERIES RESEARCH ORGANIZATION Iran Shrimp Research Center

Title : Investigation on Effects of ergosan and vibromax in control of White spot disease(WSD) and Vibriosis in shrimp Litopenaeus vannamei Apprpved Number: 4-80-12-88006 Author: Aghil Dashtian Nasab Executor : Aghil Dashtian Nasab Collaborator:B.Ghaednia,M.M,Mehrabi,M.Mirbakhsh,V.Yeganeh,I.Keshtkar, O.farsi,H.Sharifi,A.Haghighi Advisor(s) : M.Sharifrohani,M.Afsharnasab Location of execution : Bushehr province Date of Beginning : 2009 Period of execution : 5 Months Publisher : Iranian Fisheries Research Organization Circulation : 15 Date of publishing : 2009 All Right Reserved . No Part of this Publication May be Reproduced or Reference

(WSD)Litopenaeus vannamei

(WSD) Litopenaeus vannamei

Laminaria digitata & Ascophylum nodossum

(V.anguillarum1,2,V.parahemolyticus,V.harvey ,V.vulnificus)

((WSV Z1-PL1

PL1 PL10

.

)

WSV .

(P<0.05)

(P<0.05)

(Rosenbery, 2003)

(Lightner, 2003)

(WSSV)

(Wang et al.,1999; Tapay et al.,1999)

(Jimenez et al.,2000)

(Lightner,1996; chang et al.,1998;Nunan et al.,1998; Soto et al.,2001;

Rajendran et al.,1999; Hossain et al.,2001) (Chang et al.,1998; ;

Rajendran et al.,1999;Chen et al.2000;Hameed et al.,2003)

(Rajendran et al.,1999; Huang et al.,2001)

DNA

(Van Hulten,2001)

DNA (Wispoviridae)

.(Van Hulten and vlak, 2001)

(Soto et al.,2001)(Perez et al.,2005)

1502001-2

1992

(Boyad and Clay 98)

(Skjermo & Vadstein 1999)

.(Aguirre & Ascenico 2000)

(Lightner 1996)

Mursopenaeus japonicus

Vibrio flavialis

Penaeus monodon

V.harveyiiV.splendidus

(Lavilla et al.,1990)

.( Dierberg and Kiatisimkul ,1996)

(Rosenberry ,96)

(Hopkins & Villalon 1992)

(Itami et al.,1992)

(Raa et al., 1992; Sung et al.,1994; Itami et al.,1996)

(SPF) (SPR) (Pruder et al.,1995)

(Vidal et

al.,2001; Guan et al.,2003; Jiravanichpaical et al.,2004)

(Wittveldt et al.,2004)

(Kim et al.,2004)

Penaeus japonicus(Itami et al.,1998; Takashi et al.,2000)

Penaeus monodon(Song et al., 1997)

(Curdlan)

1,3

(zymosan)

MDP

(Laminaria digitata and Ascophylum nodossum)

Tumor Necrosis Factor alfa(TNF-a)(IL-8)(Peddie et al.,2002)

(RTFS)

.(Anon , undated)

Seebass .(Bagni et al., 2004)

Litopenaeus vannamei

.( Montero et al.,2005)

Laminaria digitata,

Ascophyllum nodossum

V.parahemolytius ,V.vulnifcus, V.harvey,

V.anguillarum I,II

IPL12

(WSSV)

(SPF)Liopenaeues vanammei

35ppt

Artemia franciscanaINVE

ppt pH

(1)2 34

Z1-PL1 Z1-PL1 Z1-PL1 Z1-PL1

M1-PL1M1-PL1M1-PL1M1-PL1

-Z1-PL11-Z1-PL11

PL1-PL12PL1-PL12PL1-PL12PL1-PL12

--PL1-PL10PL1-PL10

Z

M

PL

(1)

(2)

(3)

(4)

1 N6 02Z1 0/033Z20/034Z30/03 5Z3/M10/066M10/067M20/068M30/069PL10/08

10PL20/0811PL30/0812PL40/0113PL50/0114PL60/0115PL70/1216PL80/1217PL90/1518PL100/1519PL110/15

(Bio-encapsulation)

II

Instar II

25

PL1-PL10

One way ANOVATukey Honest Significant Difference (HSD)P<0.05

Litopenaeus vannamei

A

B

(Vibrio harveyii)

Z1-PL1PL1 PL10 PL12

A

B

(Challenge)

TSA

(Lee et al.,1999)

PL52(A , B)

(A1,A2,B1,B2)

CFU/ml3×10-9

Vibrio harveyii

A1 +_

A2 ++B1

__

B2_+

One way ANOVA

(HSD)

Tukey Honest Significant DifferenceP<0.05

Litopenaeus vannamei

A

B

(WSSV)

Z1-PL1PL1 PL10 PL12

A

B

Fenneropenaeus indicus

(WSD)PCR

WSSV(Challenge)(Prez et al.,2005)

PL52(A , B)

(A1,A2,B1,B2)

pH

IHHNV,TSV,WSSV

PCR

A1B1(0.5gr/1shrimp) A2

B2(0.5gr/1shrimp)(Wang et al.,1998)

WSSV

A1 +_

A2 ++

B1 __

B2_+

One way ANOVA

(HSD)

Tukey Honest Significant DifferenceP<0.05

0.00%

10.00%

20.00%

30.00%

40.00%

50.00%

60.00%

%

00.0010.0020.0030.0040.0050.0060.0070.0080.0090.01

.

(P<0.01

0%

10%

20%30%

40%

50%

60%

70%80%

90%

100%

1 2 3 4 5 6 7 8 9 10

%

A1B1

A2B2

A2

(P<0.05)

A1

A2

B1

B2

A1B1A2B2

(P<0.01)

0 %

1 0 %

2 0 %

3 0 %

4 0 %

5 0 %

6 0 %

7 0 %

8 0 %

9 0 %

1 0 0 %

1 3 5 7 9 1 1 1 3

WSSV

% C u m u la t iv e _ m o r ta l i ty C o n t ro l

% C u m u la t iv e m o r ta l i ty E r g + V ib .

A1B1 A2B2

A2

(P<0.05)

A1

A2

B1

B2

A1B1A2B2

(P<0.01)

WSSV

Litopenaeus vannamei

WSSV

Bifidobacterium termophylum

Marsupenaeus japonicusWSSV(Itami et. al.,1998)

CidofovirLitopenaeus vannameiWSSV

(Rahman et al.,2005)

(Montero et al.,2005)

(WSD)

(Biosecurity)

PCR(Nested PCR)WSSV

V.penaeicida

(Sung et al., 1994)

(Itami et al., 1994)

(WSV)

(WSD)

(Witteveldt 2006)

(WSD)

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Abstract: Complementary feedstuff extract from Laminaria digitata and Ascophylum nodosum that containing 1% alginic acids as shrimps stimulating of immune system for control of WSSV is used in this study. The test shrimps Litopenaeus vannamei in larvae stage (Z1-PL1), post larvae stage (PL1-PL10) and juvenile (from 30th day to 40th

day) fed by complimentary feedstuff comprised control group. The other condition in test and control group was the same manner. Both groups after 40 days exposed to WSSV by oral inoculation. The clinical sign and mortality recorded for 10 days. The results showed the survival rate of exposed group was higher than the control group and it was significant (p<0/05). The result also show the mortality observed in exposed group 48 hours later than the control group. This study can led us to new methods for control of White spot disease. Key words: See weed, Laminaria digitata, Aschophylum nodossum, Litopenaeus vannamei, Control, White spot disease