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worm timing paper

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Gene transcription is coordinated with, but not dependent on, cell divisions during C. elegans embryonic fate specification Gautham Nair, Travis Walton, John Isaac Murray, and Arjun Raj University of Pennsylvania Department of Bioengineering University of Pennsylvania Department of GeneDcs

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Page 1: Worm timing paper

Gene transcription is coordinated with, but not dependent on, cell divisions during C. elegans embryonic fate specification!

Gautham  Nair,  Travis  Walton,  John  Isaac  Murray,  and  Arjun  Raj  

University  of  Pennsylvania  Department  of  Bioengineering  University  of  Pennsylvania  Department  of  GeneDcs  

Page 2: Worm timing paper

RNA-FISH!

10µm!

RNA!

Page 3: Worm timing paper

Automated Lineaging!

Page 4: Worm timing paper

Gut genes!Wild type 20C!

Page 5: Worm timing paper
Page 6: Worm timing paper
Page 7: Worm timing paper

Transcription is slowed LESS?

Page 8: Worm timing paper

Transcription is slowed MORE?

Page 9: Worm timing paper

Transcription is slowed THE SAME?

Page 10: Worm timing paper

Transcription is slowed THE SAME

Page 11: Worm timing paper

Transcription is slowed THE SAME

- many other genes - clk-1 mutant slowdown

Page 12: Worm timing paper

Transcription is slowed THE SAME

- many other genes - clk-1 mutant slowdown

Page 13: Worm timing paper

Transcription is slowed THE SAME

Total Number of Cells in Embryo

Page 14: Worm timing paper

Total Number of Cells in Embryo

Page 15: Worm timing paper

Total Number of Cells in Embryo

Transcription is DEPENDENT on cell divisions?

Page 16: Worm timing paper

emb-29(g52) mutant

Total Number of Cells in Embryo

Transcription is DEPENDENT on cell divisions? NO

Page 17: Worm timing paper

Transcription is DEPENDENT on cell divisions? NO - elt-2 in E lineage - hlh-1 and unc-120 in muscle pathway

Page 18: Worm timing paper

Transcription is DEPENDENT on cell divisions? NO - elt-2 in E lineage - hlh-1 and unc-120 in muscle pathway

Page 19: Worm timing paper

Transcription is DEPENDENT on cell divisions? NO - elt-2 in E lineage - hlh-1 and unc-120 in muscle pathway

Page 20: Worm timing paper

Gene transcription is coordinated with, but not dependent on, cell divisions during C. elegans embryonic fate specification!

Page 21: Worm timing paper

Gene transcription is coordinated with, but not dependent on, cell divisions during C. elegans embryonic fate specification!

Funding:    HHMI  Life  Sciences  Research  FoundaDon  Fellowship  Burroughs-­‐Wellcome  Fund  NIH  New  Innovator  Award  Penn  Genome  FronDers  InsDtute  Pennsylvania  Department  of  Health  


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