will stem cells finally deliver without controversy? keith gary, ph.d. director of program...
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Will Stem Cells Finally Deliver Without Controversy?
Keith Gary, Ph.D.Director of Program Development
Kansas City Area Life Sciences Institute
Olathe North Life Sciences1 February 2012
What’s the Buzz?
PROMISE
POLITICS
Promise
Politics
Ethical Dilemma
Right To Life Movement
Young Scientific Field – little data
Not Amenable to Sound Bytes
Sexual Reproduction
Stages of Development
The Blastocyst
3-5 days post-fertilization
Inner cell mass = ~30 cells
Germ Layer
Ectoderm
Endoderm
Mesoderm
Fate of Embryonic Tissues
Organs and Tissues in AdultSkin epidermis, epithelium of mouth and rectum, cornea and lens of eye, nervous system, tooth enamel
Epithelium, digestive tract and respiratory system; liver, pancreas, thyroid; lining of urethra, bladder, reproductive system
Skeletal and muscular systems; circulatory & lymphatic systems; reproductive system (except germ cells); dermis of skin, lining of body cavity
Seminal Events
1981 – Mouse embryonic stem cells grown in lab
1998 – First human embryonic stem cell line
1978 – First successful in vitro fertilization
1985 – Drug stimulated superovulation
What is a Stem Cell?
Self-renewing undifferentiated cells with the ability to repair damaged tissue.
Early Stage Stem Cells(Embryonic)
Undifferentiated cells that are
pluripotent and have the potential to
become any type of tissue of the body.
Early Stage Stem Cells are Pluripotent
Produces stem cells genetically identical to donor
Not a new individual
No fertilization
Early StageStem Cells
Somatic Cell Nuclear Transfer (SCNT)
Are The Blastocysts Identical?
IVF SCNT
Adult Stem Cells
Undifferentiated cells found in a tissue or
organ that are multipotent and can
become more than one type of tissue,
but not all types.
Contains > 10 trillion cells with 250 different cell typesSome tissues continually renew themselves from adult stem cells
The Human Body
Adult vs. Embryonic Stem Cells
ES ASPluripotent Multipotent
Limiting differentiation in culture is problematic
More stable, easier to manage
Potentially immortal, unlimited supply
Lose ability to proliferate and differentiate in culture
High ethical burden, uncertain legal status
Less moral ambiguity and controversy
Alzheimer’s DiseaseCancer
Diabetes (Type I) Heart Disease
Macular DegenerationOsteoarthritis
Parkinson’s DiseaseRheumatoid ArthritisSpinal Cord Injuries
Stroke
Potential Disease Targets
Dan Perry, Executive Director of CAMR. Science (2000) 287: 1423.
U.S. Disease & Injury Statistics
Spinal Injuries– Restore function to damaged spinal cords (successful
in tests with paralyzed rats - regained ability to walk).
Examples of Potential Treatments
Type I Diabetes– Replace pancreatic cells to restore normal blood sugar.
Burn Treatment– Regenerate functional skin following severe burns.
Alzheimer’s & Other Neurological Diseases – Regenerate neural cells and restore brain/body
communication to alleviate symptoms of Alzheimer’s, ALS, multiple sclerosis & Parkinson’s.
How Does Stem Cell Research Relate to Human Cloning?
• Multiple examples of successful cloning efforts – Dolly: 1 out of 276 attempts – Mouse: 100 blastocysts transferred to wombs,
seventy-one were able to take, from which between five and sixteen fetuses developed, and eventually two or three live mice were born
• “Therapeutic Cloning”
Cloning Animals
be made illegal with substantive penalties and strict enforcement
Human Cloning Must
HumanCloning
Ethical Alternatives
Altered Nuclear Transfer
• William Hurlbut, Stanford
• Genetic alteration in adult nucleus that represses a protein necessary for trophoblast maintenance.
• Zygote unable to implant in uterus.
Ethical Alternatives
Blastomeres ESC
• Preimplantation Genetic Diagnosis - technique commonly used during IVF to detect genetic disorders.
• Long-term consequences have not been adequately addressed.
• Advanced Cell Technology report
Ethical Alternatives
Induced pluripotent stem cells (iPSCs)
• adult cells genetically reprogrammed to an embryonic stem cell–like state.
• Although iPSCs meet the defining criteria for pluripotent stem cells, it is not known if they differ from embryonic stem cells in clinically significant ways.
• express genes and factors important for maintaining the defining properties of embryonic stem cells.
Induced pluripotent stem cells (iPSCs)
What the scientific community knows about stem cells
• The most “plastic” cells are early stage
• Some adult stem cells have differentiative potential
• Other sources exist (Fetal Cord Blood)
• Current stem cell lines approved for government funded studies are inadequate to drive research forward
Clinical Trials
Geron Initiates Clinical Trial of Human Embryonic Stem Cell-Based Therapy - October, 2010
FDA OKs 1st Embryonic Stem Cell TrialJanuary, 2009
Second human embryonic stem cell clinical trial to startNovember, 2010
FDA Delays Clinical Trial of Embryonic Stem Cells May 2008
Take Home Message
• Stem cells have great potential benefits for agriculture and biomedical sciences.
• Efficiencies are very low and research needs to be done before stem cells will be of benefit to society.
• Its important for scientists to be proactive and educate the general public, media and government.
Additional Information
• National Institutes of Health http://stemcells.nih.gov/info/basics/
• University of Wisconsin http://www.news.wisc.edu/packages/stemcells
• Coalition for the Advancement of Medical Research http://www.camradvocacy.org/stem_cell_news.cfm